Aloe arborescens 추출물의 Lacticaseibacillus rhamnosus 발효에 따른 항산화 및 자외선 보호 효과

홍영선,박기수 국제차세대융합기술학회 2024 차세대융합기술학회논문지 Vol.8 No.5

본 연구에서는 Aloe arborescens (A. arborescens) 발효 추출물의 항산화능과 자외선 보호 효과를 측정하였다. DPPH와 ABTS assay에서 radical의 양은 A. arborescens 발효 추출물의 농도에 비례하여 감소함을 확인하였으며, EC50을 계산한 결과 발효 전과 비교하여 각각 약 91.3%, 약 92.2%의 항산화능이 증가한 것으로 나타났다. 발효 전과 후의 페놀성 물질과 플라보노이드의 농도를 비교한 결과, 발효 전과 비교하여 발효 후의 페놀성 물질의 경우 28.5%의 농도 증가를 보였으며, 플라보노이드의 경우 22.4%의 농도 증가를 보였다. 세포독성을확인한 결과 A. arborescens 발효 추출물은 CCD-986sk와 HFDPC 모두에서 세포 독성을 나타내지 않았으며, 자외선 조사 결과 자외선에 의한 세포 사멸을 막는 것을 확인하였다. 또한, CCD-986sk의 콜라겐 감소를 농도 비례적으로 막는 것을 확인하였으며, HFDPC의 ROS 생성 역시 농도 비례적으로 막는 것을 확인하였다. 이를 바탕으로 자외선을 가장 직접적으로 받게 되는 머리 및 두피에 대한 보호 화장품의 원료로서 A. arborescens 발효 추출물의 가능성을 확인할 수 있었다. In this study, the antioxidant activity and UV protection effect of fermented A. arborescens extract were determined. In the DPPH and ABTS assay, the amount of radical decreased with increasing concentration of fermented A. arborescens extract. Based on the calculated EC50 of the non-fermented and fermented extract, we found that the antioxidant capacity of fermented extract increased by about 91.3% and 92.2% compared to non-fermented one, respectively. Through the evaluation of the total phenolic contents and flavonoids before and after fermentation, a 28.5% increase in the concentration of total phenolic contents and a 22.4% increase in the concentration of flavonoids was obtained after fermentation. Furthermore, the fermented extract did not exhibit cytotoxicity against both CCD-986sk and HFDPC, and prevented UV-induced cell death in a concentration-dependent manner. Similarly, it prevented not only collagen reduction of CCD-986sk but also ROS generation of HFDPC in a concentration-dependent manner. Based on these findings, we confirmed the effectiveness of the fermented extract of A. arborescens as an ingredient in protective cosmetics for the head and scalp, which are most directly subjected to ultraviolet radiation.

Talking to the patients

홍영선,Hong, Yeong-Seon 한국가톨릭호스피스협회 2003 호스피스 Vol.26 No.-

초대석-아시아 태평양 호스피스 학회(Asia pacific Hospice Palliative Care Network, APHN)

홍영선,Hong, Yeong-Seon 한국가톨릭호스피스협회 2007 호스피스 Vol.40 No.-

A Study of High-Risk Drinking Patterns Among Generations Based on the 2009 Korea National Health and Nutrition Examination Survey

홍영선,천성수,윤미은,Lydia Sarponmaa Asante,주재신 질병관리본부 2014 Osong Public Health and Research Persptectives Vol.5 No.1

Objectives: The aim of this study was to identify how the drinking patterns of a generation on the paternal side affect those of the next generations by estimating the number of high-risk drinkers by generation according to the Alcohol Use Disorder Identification Test. Methods: Data were selected from the 2009 Korea National Health and Nutrition Examination Survey conducted by the Korea Centers for Disease Control and Prevention and were analyzed using SPSS 18.0. Results: Later generations started drinking earlier (62.4%, 71.8% and 91.1%, respectively). Themajority of the second generation consumedmore than 2e4 drinks a month (83.7%), but only a small proportion experienced difficulty in everyday life (9.6%), felt repentance (9.6%), or experienced memory loss (17.9%) after drinking. Unmarried third-generation adults with high-risk-drinking fathers reported more frequent alcohol consumption [odds ratio (OR) 1.441), greater amounts on one occasion (>7 cups for men, OR 1.661; > 5 cups for women, OR 2.078), temperance failure (OR 2.377), and repentance after drinking (OR 1.577). Unmarried thirdgeneration adults with high-risk-drinking grandfathers consumed greater amounts of alcohol on one occasion (OR 3.642), and unmarried third-generation women more frequently consumed large amounts of alcohol (>5 cups, OR 4.091). Unmarried thirdgeneration adultswith high-risk-drinking fathersweremore likely to exhibit high-risk drinking patterns (OR 1.608). Second-generation individuals froma high-risk-drinking first generation were more likely to engage in high-risk drinking (OR 3.705). Conclusion: High-risk drinking by a generation significantly affects the high-risk drinking patterns of subsequent generations.

위암의 선행화학 요법

홍영선,박조현,Hong, Young-Seon,Park, Cho-Hyun 대한위암학회 2005 대한위암학회지 Vol.5 No.3

위암은 한국에서 가장 발생빈도가 높은 암이며 암 사망원인의 2위를 차지하고 있다. 치료법 중에서 수술이 유일하게 완치의 기회를 제공하나, 진행 암에서는 근치적 절제(R0 resection, R0 절제)수술 후에도 약 50%의 환자에서 재발을 보여, 보다 효과적인 치료법의 개발이 필요하다. 선행화학요법은 병소가 급격하게 성장, 팽창하는 것을 막고 내성을 지닌 세포의 출현을 예방하며 그렇게 함으로써 완치의 기회를 늘리고, 암의 국소 조절을 더 잘 함으로서 수술의 범위를 줄여 수술로 인한 부담을 줄이며, 절제 불가능한 암을 절제 가능한 암으로 만드는 것이 목표이다. 따라서 치유절제가 불가능할 것으로 판단되었던 위암의 병기를 낮추어 R0 절제 후 완치율을 높일 것으로 기대되고 있으며 계속되는 연구에서 수술로 인한 이환율과 사망률을 높이지 않는 것으로 나타나고 있다. 선행 화학요법에 사용할 수 있는 항암제는 여러 가지이나 가장 높은 효과를 보이는 항암제가 결정되지 않았고 또 항암제를 투여하는 시기, 선행 화학요법 후에 재발을 방지하기 위한 항암제의 복강 내 투여나 방사선 치료의 병용 등이 복막 전이와 국소재발을 줄이는 데 도움이 되는지 등이 향후 시행될 연구에서 규명되어야 하겠고, 또 적절한 수술방법에 관하여도 합의가 이루어져야 하겠다. 선행 화학요법과 관련된 여러 가지 의문들을 해결하기 위한 노력은 다기관이 참여하는 전향적 무작위 3상 임상연구를 통하여 이루어져야 할 것으로 생각된다. Gastric cancer is the most prevalent cancer in Korea and comprises the second cause of cancer death. Surgery only can provide chance of cure, but most locally advanced cancers recur after a curative resection, even though important advances in the surgical and nonsurgical treatments of gastric cancer have taken place. Preoperative chemotherapy theoretically can provide the advantages of reducing the bulk of tumor, which might improve the R0 resection rate, and of treating micrometastases early. Also, preoperative chemotherapy is expected to render unresectable tumors resectable without increasing postoperative morbidity and mortality. There are many new chemo-therapeutic agents available for the treatment of advanced gastric cancer, but still the most effective agent, the optimal time and number of cycle for administration are still not known. The addition of postoperative chemotherapy through an intraperitoneal route and/or radiotherapy might affect the outcome of surgery favorably, but that hasn't been proved yet. A multicenter prospective randomized phase III trial should be peformed to answer for those questions and to improve the curability of gastric cancer treatment.

다발성 골수종, 왜 생기나

홍영선,Hong, Yeong-Seon 한국건강관리협회 1995 건강소식 Vol.19 No.8

현장보고-아시아 태평양 호스피스 네트웍 참가 보고

홍영선,Hong, Yeong-Seon 한국가톨릭호스피스협회 1997 호스피스 Vol.8 No.-

영국의 호스피스 기관 방문기

홍영선,Hong, Yeong-Seon 한국가톨릭호스피스협회 1998 호스피스 Vol.10 No.-

위암세포에서 세포유착물질의 발현 및 위암세포의 복막 내피세포에 대한 결합 능에 미치는 retinoicacid의 영향

홍영선,박조현,박진노,이경식,김인철,Hong, Young Seon,Park, Cho Hyun,Park, Jin-No,Lee, Kyung Shik,Kim, In Chul 대한면역학회 2001 Immune Network Vol.1 No.1

Background : Peritoneal metastasis is one of the maj or types of the stomach cancer recurrence and the role of the adhesion molecules is thought to be very much important in this event. Retinoic acid (RA) has been known to induce the growth inhibition and differentiation of various malignancies, and apoptpsis and the change of expression of adhesion molecules have been reported to be involved in the action of RA. Methods : We studied the adhesion abilities of SNU-1, SNU-5, and SNU-6 cells to the peritoneal endothelial cells as well as the expression of the adhesion molecules (CD44, ICAM-1) in Western blot analysis. And also we studied the expression of apoptosis and the change of expression patterns of the various isoforms of CD44 and the change of the adhsion abilities of the cell line cells after RA treatment. Results: CD44 was expressed in SNU-5 and -16, together with an isoform in SNU-16. ICAM-1 was not expressed in any of the cell line cells tested. After the treatment of RA in the concentration range of $1-5{\times}10^{-5}M$ to three stomach cancer cell lines, growth inhibition, apoptosis and the change of expression of the CD44 were noted. After RA treatment, the expression of CD44H was weakly increased in SNU-1, and was markedly increased in SNU-5. In SNU-16, the expression of CD44H was decreased while that of CD44E were markedly increased. The adhesibility of cells to peritoneal cells was increased in relation with the increase of the CD44H expression, which shows the fact that the adhesibility of tumor cells to peritoneal mesothelial cells is mediated by CD44H recognizing hyaluronic acid. Conclusion : RA induces growth inhibition of stomach cancer cell line cells and increase the adhesiblity of stomach cancer cell line cells to peritoneal mesothelium. It is believed that RA decreases the metastatic ability of stomach cancer cells by upregulating the CD44H expression.

한국형호스피스모델

홍영선,Hong, Yeong-Seon 한국가톨릭호스피스협회 1999 호스피스 Vol.15 No.-