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- 저자 : 朴魯東 (검색결과 6 건)
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水分障碍 및 鹽藏碍하에서 水稻體중 酵素水準 및 有機代謝産物과 無機이온 함량의 변화
朴魯東 한국응용생명화학회 1982 Applied Biological Chemistry (Appl Biol Chem) Vol.25 No.3
Pot에 栽培한 水稻 진주벼와 이리 348號에 수분 결핍과 염장애를 유도한 다음 몇가지 有機代謝産物과 無機이온의 含量을 測定하고 관계되는 몇가지 酵素活性을 測定하여 比較檢討하였다.非耐監性으로 알려진 이리 348號가 不適環境條件에서 水分障碍區에서만 蓄積되었다. 조단백질에 대한 有機溶質의 比는 진주벼의 不適環境區에서만 증가한 반면 일 348號에서는 변화가 없었다.不適環境區에서 活性이 增加한 酵素로는 protease, ∝-amylase, phosphorylase가 있으며, 특히 조단백질에 대한 총유리아미노산의 比의 增加는 protease에 의한 단백질의 加水分解에 기인했다.Na^+과 CI^-의 함량은 이리 348號에서 진주벼에서 보다 높았다. Na^+/Ca^2+과 양이온의 一價/二價의 값들은 진주벼에서 항상 낮았고, K^+/Na^+은 진주벼에서 높았다. 그러므로 이러한 값이 鑒害와 早麴에 대한 저항성과 어떠한 相關이 있는지 더욱 硏究할 필요가 있다고 본다. Two rice cultivars, Jinju and Iri 348, were used to compare the changes in the contents of some organic metabolites and ions and in some enzyme levels under water-and salt-stressed conditions. The water loss and proline accumulation under water and salt stresses were accelerated more in the salt?sensitive cultivar Iri 348 than in the salt-tolerant Jinju. The contents of crude protein, total free amino acids, proline and polyphenols increased under water-or salt-stressed rice, but that of reducing sugar increased under water stress only. The water-and salt?stresses induced the high ratio of low molecular organic so-lutes to crude protein in Jinju but not in Iri 348. The ratio of total free amino acids to crude protein increased under the stressed conditions was likely due to high protease activity. The contents of Na^(+) and Cl^(-) were higher in Iri 348 than in Jinju. Iri 348 had higher values of Na^(+)/Ca^(2+) and monovalent/divalent of cations, but lower of K^(+)/Na^(+) than Jinju Rice. The further studies should emphasize to set the correlations between these ratios and tolerance to water and salt stresses among rice cultivars
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김 養殖網의 파래 驅除를 위한 有機酸 製劑의 開發 硏究
徐鎔澤,金容雄,鄭美羅,金廣植,朴魯東 全南大學校 農業科學技術硏究所 1996 農業科學技術硏究 Vol.31 No.-
For control of green algae (Enteromorpha linza) from the cultivating laver (Porphyra) nets, this study has been conducted to develop organic acid algicide formula which contain organic acids as main components. 1. Among 50 organic compounds tested, 12 organic acids were most effective in induction of the color chango of the blades of green algae and Porphyra when the blades were immersed in 0.25-3% organic acid seawater solutions. The pH of the 0.25-3% organic acid seawater solutions were measured. 2. The photosynthetic activities of green algae were strongly inhibited in 1.5% organic acid seawater solutions of citric acid, malic acid and oxalic acid, while those of Porphyra were much less affected. 3. Nitric acid, ammonium chloride and phosphoric acid were selected as synergists to strengthen the algicidal effect of the selected organic acids. 4. 10 algicidal formulas were formulated, which contained citric acid, malic acid or oxalic acid as major components, and phosphoric aicd, nitric aicd or ammonium chloride as synergists. Their effectiveness in the induction of color change and the photosynthetic activities of the blades of green algae were tested and compared with those of the commercial formulas. 5. The most effective application procedure was found out the spray of the organic acid formula solutions to the laver nets immediately after exposure from seawater and the returning to seawater 1 hr after.
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金正鳳,安承姬,朴魯東 全南大學校 農漁村開發硏究所 1988 農業科學技術硏究 Vol.23 No.-
식물의 중요한 생장조절물질인 IAA의 대사를 규명하기 위한 연구의 기초작업으로서, 26가지 표준 인돌 화합물의 hRf값과 發色樣狀을 실리카겔 TLC에서 5가지 發色試藥과 8가지 展開溶媒系를 사용하여 調査하였다. Ehmann試藥은 인돌類의 檢索에 예민한 반응을 보였으며, 암모니아를 함유하는 展開溶媒系가 보다 좋은 分離能을 보여주었다. 그러나, 여러 종류의 인돌 化合物을 한가지 展開溶媒系에서 뚜렷하게 서로 분리시키기는 어려운 일이었다. hRf-values and colors of 26 standard simple indorse derivatives were studied on silica gel layers of 250㎛ thickness using 5 spray reagents and 8 solvent systems. Ehmann reagent, a mixture of van Urk and Salkowski reagents. was sensitive and specific for silica gel thin-layer chromatographic detection and identification of indole derivatives. Ammoniacal solvent has proved satisfactory for separation of auxins. But, a sharp resolution of a number of indole derivatives was not possible in the solvent systems tested.
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Carbamate系 殺蟲濟 MIPC(o-Isopropylphenyl-N-methylcarbamate) 粉濟의 安定性에 關한 硏究
孫炫洲,朴魯東,朴昌奎 서울大學校 農科大學 1980 서울대농학연구지 Vol.5 No.2
Effects of moisture, pH, surface acidity and stabilizers on the stability of MIPC in the 2% dust formulations have been studied. MIPC dust formulations containing different levels of moisture and stabilizers were perpared from locally produced carriers, clay, talc and kaolin. The formulated MIPC dust formulation were subjected to thermo-accelerated test. The results are summarized as follows: 1. As regard to MIPC stability to moisture content, clay or kaolin based MIPC dust formulations was most stable when the carriers were used as received. Talc based MIPC dust was, however, most stable when moisture content of the carrier was adjusted to 1%. 2. Effects of stabilizers were best demonstrated when HMT, urea and PAP were added to talc based MIPC formulations. In general, stabilizing effects of varioul stabilizers were meagre in the clay based dust. The PAP alone had pronounced stabilizing effect in the kaolin based formulations. 3. Among three carriers, clay excelled itself over compatibility with MIPC in the dust formulations. 4. concentration of either HMT or urea at 5% showed greater stabilizing effect compared with those at 3%. 5. Among laboratory formulated MIPC dust formulations, clay I+MIPC+urea(5%), clay I+MIPC+urea(3%), clay II+MIPC+HMT(5%) and talc I+MIPC+urea(5%) proved equal or even superior to the commercial MIPC dust by the thermo-accelerated test.
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Polychlorinated Biphenyls(PCBs) 存在下에 有機鹽素系 殺虫劑의 殘留分 分析
朴昌奎,李春寧,朴魯東 한국응용곤충학회 1977 한국응용곤충학회지 Vol.16 No.4
Polychlorinated Biphenyls (PCBs) interfere with gas chromatographic analysis of multiple organochlorine pesticide residues. In the present work, existing Florisil column chromatographic method has been modified as to improve separation of organochlorine pesticides and their metabolites from PCBs. It was amply demonstrated that separation of Heptachlor epoxide Dieldrin, p.p-DDD, p.p'-DDT from PCBs such as Aroclor 1254 is complete and recovery of the pesticides is found quantitative. Aldrin and Heptachlor in the Aroclor eluant can be separately analyzed by comparison of the chromatographic pattern of standard Aroclor 1254 with that of Aroclor 1254 a dmixed with the two pesticides. The Florsil column technique can be utilized in the routine evaluation of the organochlorine pesticide residues by gas chromatography. Polychlorinated Biphenyls (PCBs)는 유기염소계 농약잔류성분의 GLC분석에 간섭하는 물질이다. 본 실험에서는 PCBs의 존재하에 대표적인 유기염소계 농약 및 이들의 주요대사물의 분석을 목저르오 Florisil column상에서 PCBs와 농약성분의 분리를 시도하였다. 활성화한 Florisil column에서 , Heptachlor, epoxide, Dielerin, p.p'-DDD, p.p'-DDT와 Aroclor 1254는 완전히 그리고 정량적으로 분리하여 GLC로 분석할 수 있었다. Heptachlor와 Aldrin은 Aroclor와 함께 elution되나 Chromatogram 상에서 정량이 가능하였다. 본 시험에서 사용한 Florisil column chromatography는 많은 시료를 대상으로 한 잔류농약의 효과적 분석에 사용될 수 있다.
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