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LPS로 자극한 RAW 264.7 세포에서 중국 연변에 자생하는 약용 식물 에탄올 추출물의 항염증 효과 연구
박예진 ( Yea-jin Park ),서종환 ( Jong-hwan Seo ),길태영 ( Tae-young Gil ),천세윤 ( Se-yun Cheon ),박인철 ( Ren-zhe Piao ),이상우 ( Sang-woo Lee ),차윤엽 ( Yun-yeop Cha ),안효진 ( Hyo-jin An ) 대한본초학회 2018 大韓本草學會誌 Vol.33 No.6
Objectives : This study was fulfilled to investigate nominee materials as anti-inflammatory agent from ethanol extract of Chinese medicinal plants in Yanjin. Among the 20 candidates, we selected most effective one, the ethanol extract of Cicuta virosa L. (CVL). The mechanism underlying the anti-inflammatory effects of CVL is not clearly identified as yet. Accordingly, we clarified the anti-inflammatory effects of CVL and its underlying molecular mechanisms in LPS-stimulated RAW 264.7 macrophages. Methods : RAW264.7 macrophages were incubated with CVL (12.5, 25, or 50 μM) and/or lipopolysaccharide (LPS) (1 ㎍/㎖). Cytotoxicity was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay and the level of nitric oxide (NO) production was measured with Griess reagent. The prostaglandin E2 (PGE2) production was measured with enzyme immunoassay kits and the protein expression of inducible nitric oxide synthase (iNOS) was determined using Western blot analysis. Results : Among the 20 ethanol extract of Chinese medicinal plants of Yanjin tested, CVL significantly reduced the production of NO in a dose-dependent manner via inhibition the protein expressions of iNOS without cytotoxicity on the LPS-stimulated RAW 264.7 macrophages. In addition, CVL also effectively declined the production of PGE2 in LPS-simulated RAW 264.7 macrophages. Conclusions : Taken together, these data presented in this study demonstrate that CVL possesses anti- inflammatory activity by suppressing the production of pro-inflammatory mediators NO and PGE<sub>2</sub>, and pro- inflammatory protein iNOS expression in LPS-stimulated RAW 264.7 macrophages.