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曺準承,李光烈 慶北大學校 醫科大學 1985 慶北醫大誌 Vol.26 No.1
〔U-^14C〕glucose의 탄소원자가 지방조직의 지질에의 편입에 대하여 절식 및 고당질식이 투여가 어떠한 영향을 미치는가를 쥐를 사용해서 조사해본 결과는 다음과 같았다. 지방조직의 지질에 glucose 탄소원자의 편입속도는 정상섭식상태에 비하여 24시간 절식상태에서 약 3분의 1로 현저하게 감소되었으며, 절식후 고당질식이를 24시간 투여하면 이편입속도는 약 2배로 증가되었다. 그러나, 고당질식이로 장기간 사육하면 이편입률은 오히려 저하되는 경향을 보였다. 이상의 결과는 쥐에서 glucose의 지질합성으로의 이용이 기아상태에서는 현저하게 저하되고 많은 당질을 섭취할때는 뚜렷하게 증가되나 장기간 섭취하면 오히려 저하됨을 뜻하고 있다. The object of present study was to evaluate the effect of high-carbohydrate diet on the rate of lipogenesis from glucose carbon in adipose tissue of rats using 〔U-^14C〕glucose under four different nutritional state. All rats were injected 4 microcuries of 〔U-^14C〕glucose mixed with 10mg of glucose per body weight of 100g, three hours later the amount of lipid extracted from mesenteric adipose tissue of 500mg was checked, and radioactivity of the 〔^14C〕lipid was measured with gas flow counter. The rate of lipogenesis in adipose tissue was significantly low in rats fasted 24 hours. However, the reverse was true in case of high-dietary carbohydrate treatment to 24-hour fasted rats compared to rats fed control diet. Rats previously fed the high-carbohydrate diet for 4 weeks showed general decrease in the rate of lipogenesis although lipid content was more elevated in adipose tissue than that of the control diet rats. These results indicated that the rate of lipogenesis in adipose tissue changes significantly under various nutritional states, especially high-carbohydrate diet leads to elevate the rate of lipogenesis but prolonged administration of the diet might tend to depress the rate of lipogensis with increase of lipid content in adipose tissue.
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조준승,정태호,곽춘식,김중영,Jo, Joon-Seung,Chung, Tai-Ho,Kwak, Chun-Sik,Kim, Choong-Young 생화학분자생물학회 1977 한국생화학회지 Vol.10 No.1
인삼 성분을 메탄올로써 추출하고 이것이 쥐에 있어서 당질의 산화와 당의 생성 즉 glycogenesis 및 glyconeogenesis 그리고 당 신생반응의 첫 단계 반응을 조절하는 효소라고 믿고 있는 간의 phosphoenolpyruvate carboxylkinase의 활성에 어떠한 영향을 미칠것인가를 조사하여 보았다. 인삼 추출물을 쥐 체중 100g당 5mg이 되도록 생리적 식염수 0.5ml에 녹여서 1일 1회 4일간 복강내로 미리 투여하였으며 대조군에는 같은 양의 식염수만 주입하였다. 이것을 다시 계속 섭식시킨 군과 24시간 굶긴 군으로 나누어서 인삼 추출물을 투여한 1시간뒤에 glucose-$^{14}C$ 또는 amino acid mixed-$^{14}C$를 주입하여 호기중애 배출되는 $^{14}CO_2$ 양을 검출하고 또 3시간 후에 간속의 glycogen과 phospho-enolpyruvate caboxykinase에 대해서 검토하였다. 이 실험 결과 인삼은 glucose의 산화를 현저히 증가시켰으며 이 효과는 기아상태에서 더욱 촉진적으로 작용하였다. 그러나 인삼은 간에서의 glycogenesis나 glyconeogenesis에 대해서는 효능이 그리 없는것 같았다. 즉 인삼투여군은 대조군에 비하여 간의 glycogen농도나 $^{14}C$ 화합물의 glycogen에의 편입속도가 다같이 별 차이를 나타내지 않았다. 그리고 간의 phosphoenolpyruvate carboxykinase의 활성에도 인삼의 별 영향을 미치지 않았다. Cultivated dry ginseng root was pulverized and extracted with methanol. The extractive was washed with ether three times and filtered. The filtrate was concentrated under reduced pressure and lipophilized to be dried. Sprague-Dawley rats weighing 200~220g were divided into the ginseng and the saline administered groups. The ginseng group were received daily 5mg per 100g body weight of the ginseng extract in 0.5ml of saline peritoneally for 4 days, and the saline group received the same amount of saline. The ginseng and saline groups were further divided into the fasted group, maintained in fasting state for 24 hours before the last administration of ginseng, and the fed group, which was kept in feeding during the experiment. A hour later the administration of ginseng or saline to the corresponding, 4 uc of glucose-$^{14}C$ with carrier 100mg glucose per 100g of body weight or $4\;{\mu}c$ of amino acid mixed-$^{14}C$ with carrier 20mg L-alanine per 100g of body weight were injected peritoneally for the correspond group and control. Three hours after the administration of glucose or amino acid rats were killed to be determined the content of liver glycogen and phosphoenolpyuvate carboxykinase, To estimate the oxidation rate of glucose, rats were put in a metabolic cage immediately after the injection of glucose. The expired carbon dioxide was collected into barium hydroxide solution and the radioactivity was counter by gas flow counter. It was revealed that the ginseng root extract increased the oxidation of glucose in rat in the early period of the time after a glucose injection. This effect was more prominent in the fasting state. Ginseng, however, does not so much effect on glycogensis and glyconeogenesis when the animal was at the state of feeding or fasting since glycogen content and the rate of glycogen synthesis from glucose-$^{14}C$ or amino acid mixed-$^{14}C$ in ginseng administered groups showed no fructuation compared with that of the control. Activity of phosphoenolpyruvate carboxykinase considered as a regulatory enzyme for glyconeogenesis was not changed by the adminisration of ginseng both the time of feeding and fasting.
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개구리(Rana nigromaculata)肝細胞內 Phosphoenolpyruvate Carboxykinase의 分布에 關하여
曺準承,申鉉鑽 慶北大學校 1975 論文集 Vol.20 No.-
In the present study the existence and subcellular diststribution of the phosphoenolpyruvate (PEP) carboxykinase in the liver of the frog were investigated and compared with those in the livers of the several animals. The results thus obtained are as follows. 1. The considerable amount of PEP carboxykinase was detected from the liver of the frog, the activity of this enzyme was 50.100 cpm/min /g liver, and this enzyme was contained in the mitochondria and the cytoplasm of the liver cell of the frog. 2. About 74% of PEP carboxykinase in the frog liver cell was contained in the mitochondria, and 26% was in the cytoplasm. The mitochondria in the frog liver cell contained three times as much enzyme as the cytoplasm. 3. The activity of PEP carboxykinase was highest in the liver of the chicken and became lower in the order of the rabbit, dog, cattle, frog and mouse. In the liver cells of the chicken and frog the mitochondria contained more enzyme than cytoplasm. However, in the liver cell of mouse the cytoplasm contained more enzyme than the mitocondria. In the case of the rabbit, dog and cattle, the amount of this enzyme in the mitochondria and cytoplasm was similar.
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조준승,정태호,곽춘식,김중영 ( Joon Seung Jo,Tai Ho Chung,Chun Sik Kwak,Choong Young Kim ) 생화학분자생물학회 1977 BMB Reports Vol.10 No.1
Cultivated dry ginseng root was pulverized and extracted with methanol. Theextractive was washed with ether three times and filtered. The filtrate was concentrated under reduced pressure and lipophilized to be dried. Sprague-Dawley rats weighing 200∼2208 were divided into the ginseng and the saline administered groups. The ginseng group were received daily 5㎎ per 1008 body weight of the ginseng extract in 0.5㎖ of saline peritoneally for 4 days, and the saline group received the same amount of saline. The ginseng and saline groups were further divided into the fasted group, maintained in fasting state for 24 hours before the last administration of ginseng, and the fed group, which was kept in feeding during the experiment. A hour later the administration of ginseng or saline to the corresponding, 4 μc of glucose-^(14)C with carrier 100㎎ glucose per 1008 of body weight or 4 μc of amino acid mixed-^(14)C with carrier 20㎎ L-alanine per 1008 of body weight were injected peritoneally for the correspond group and control.. Three hours after the administration of glucose or amino acid rats were killed to be determined the content of liver glycogen and phosphoenolpyuvate carboxykinase. To estimate the oxidation rate of glucose, rats were put in a metabolic cage immediately alter the injection of glucose. The expired carbon dioxide was collected into barium hydroxide solution and the radioactivity was counter by gas flow counter. It was revealed that the ginseng root extract increased the oxidation of glucose in rat in the early period of the time after a glucose injection. This effect was more prominent in the fasting state. Ginseng, however, does not so much effect on glycogensis and glyconeogenesis when the animal was at the state of feeding or fasting since glycogen content and the rate of glycogen synthesis from glucose-^(14)C or amino acid mixed-^(14)C in ginseng administered groups showed no fructuation compared with that of the control. Activity of phosphoenolpyruvate carboxykinase considered as a regulatory enzyme for glyconeogenesis was not changed by the administration of ginseng both the time of feeding and fasting.
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