길항세균(拮抗細菌) Pseudomonas gladioli를 이용(利用)한 딸기 시들음병(病)의 생물적(生物的) 방제(防除)

문병주,로성환,조종담 한국식물병리학회 1990 Plant Pathology Journal Vol.6 No.4

Bacillus megaterium N4에 의한 들깨 균핵병 (Sclerotinia sclerotiorum)의 생물학적 방제

문병주,김현주,송주희,이광열,백정우,정순재 한국생명과학회 2004 생명과학회지 Vol.14 No.5

This study was investigated the occurrence of sclerotinia rot caused by Sclerotinia sclerotiorum at the major perilla cultivating area, Gangdong-dong, Gangseo-gu, Busan in 1998. The incidence of this disease ranged from 8.1 to 28.3% at Gangdong-dong area during the growing seasons. Symptoms of the disease initially appeared damping-off of infected stems and soft-rot on the leaves of perilla. Under the relatively high humidity, abundant white mycelia of the pathogen formed on the lesion developed into black sclerotia later and the infected leaves were finally fell down. Sixteen isolates, Sl-S16, isolated from diseased lesions showing typical symptoms, and pathogenicity was tested using mycerlial disks. Among them, S2 isolate showing the most strong pathogenicity was selected and identified as Sclerotinia sclerotiorum on the basis of morphological and cultural characteristics. For biological control, an antagonistic bacteria, N4 isolate which effectively inhibited not only mycelial growth of S2 isolate but also suppress sclerotinia rot on the pot assay, was selected and identified as Bacillus megaterium according to Bergey's manual and API system., Wettable powder type, N4 formulation using B. megaterium N4 isolate was developed and estimated its control effect on perilla crops in a plastic house. As a results, N4 formulation which applied before 3 days inoculation of pathogen was effectually controlled Sclerotinia rot as the control value of 98.0%, was more effective than chemical fungicide, benomyl showing the control value of 78.0%. This is the first report of wettable powder formulation as a biocontrol agent using B. megaterium N4 against Sclerotinia rot caused by S. sclerotiorum on perilla.

딸기 겹무늬 病菌, Dendrophoma obscurans(Ell. & Ev.)Anderson의 柄子殼 및 柄胞子 形成에 미치는 몇가지 發育因子의 영향

曺鐘澤,文炳周 東亞大學校 1981 東亞論叢 Vol.18 No.1

몇 가지 發育因子가 딸기 겹무늬病菌, Dendrophoma obscurans(Ell. & Ev.) Anderson 의 柄子殼과 柄胞子 形成에 미치는 영향에 대하여 비교 검토한 結果를 要約하면 다음과 같다. 1. 本 菌의 柄子殼과 柄胞子는 순수 合成 培地에서는 전연 形成되지 않았다. 2. 供試한 7種의 發育因子 中에서 柄子殼 形成에 가장 效果的인 것은 malt extract 이었으며 다음은 yeast extract, thiamine-HCI 順이었으나 柄胞子는 thiamine-HCI 添加에 의하여 가장 많이 形成되었다. 그 적정 濃度는 malt extract가 1∼5%, yeast extract는 1%,, thiamine은 400r/1 이었다. 3. 柄子殼과 柄胞子의 多量 形成에 적합한 培地 조성은 Czapek's Agar의 炭素源을 gluose로 대치하고 여기에 thiamine 100r/1와 malt extract 0.5%를 混合 添加할 경우 가장 效果的이었다. 4. 柄子殼 및 柄胞子 形成에 대한 發育因子의 促進效果는 S-2 菌株가 他菌株에 비하여 有意的으로 컸다. The experiments were conducted to clear the effects of growth factors on the production of pycnidia and pycniospores of Dendrophoma obscurans (Ell. & Ev.) Anderson. The results obtained are summarized as follows: 1. The fungus failed to form mature pycnidia on synthetic media such as Elliot's Agar, Czapek's Agar and Richara's Agar. 2. Of the 7 growth factors tested, malt extract was the best for pycnidia formation, and followed by yeast extract and thiamine-HCI, while thiamine-HCI was the most effective for formation of pycniospores. The proper concentration of malt extract, yeast extract and thiamine-HCI were 1∼5%, 1% and 400r/1, respectively. 3. Malt extract 5g/1 with thiamine-HCI 100r/1 was the most effective in increasing the amount of formation of pycnidia and pycniospores, when the sugar source in Czapek's Agar as the basal medium was replaced with glucose. 4. Of 4 isolate used, S-2 isolate produced more pycnidia and pycniospores than did the other isolates, when the growth factors were added in the basal medium.

멀티미디어시대를 향한 위성 개발동향

문병주,Mun, Byeong-Ju 한국전자통신연구원 1996 전자통신동향분석 Vol.11 No.4

멀티미디어는 영상, 음성, 문자, 도형 등 표현 미디어의 통합화, 네트워크화, 인터액티브화라는 특징을 지니고 있다. 위성통신은 이를 지원할 수 있는 광역성, 광대역성, 동보성 등의 특징을 지니고 있어 멀티미디어 서비스를 조기에 실현할 수 있는 통신수단으로 주목되고 있다. 본 고는 이러한 관점에서 위성 개발동향을 살펴보기로 한다.

Sclerotinia sclerotiorum(Lib.) de Bary에 의한 들깨 菌劾病과 그 新寄主

曺鐘澤,文炳周 東亞大學校生命資源科學大學附設 農業資源硏究所 1994 農業生命資援硏究 Vol.3 No.2

최근 부산근교의 하우스재배 지대에서는 채소용으로 재배되는 들깨의 줄기에는 잘룩병이 나타나고 잎에는 갈색∼흑갈색의 병반이 형성되어 습하면 연부현상이 나타나며 ???병부에는 菌核이 형성되는 신병해가 발생하여 그 피해가 막심하다. 본 병균의 形態와 病徵을 조사한 결과 Sclerotinia sclerotiorum (Lib.) de Bary로 同定되었는데 그 특징은 다음과 같다. 자낭내의 자낭포자는 그 크기가 동일하고 자낭포자는 2핵이며 자낭포자의 길이와 나비의 비가 2.0보다 크고 자낭반의 皮層은 prismatica조직으로 구성되어 있었다. 菌核은 그 크기가 평균 2.8mm로서 PDA평면배에서 주로 균총 가장자리에 윤상으로 형성되고, 균핵의 皮層은 암갈색 원형의 세포가 2∼6층으로 구성되고 그 내부는 oblita조직으로 구성되며 皮層외부에는 tomentum hypha가 형성되지 않았다. 人工接種에 의하여 본 병원균에 대한 新寄主 10種과 우리나라 미기록 기주 2종을 발견하였다. A new disease of perilla was recently found in the area of house culture near Pusan and it is causing a serious damage of crops in the area. Symptoms of the disease are damping-off of infected stems and also occur on leaves as showing brown to dark brown spots, which is caused a soft rot in wet conditions. Sclerotia of the pathogen is also formed on the infected stem. The pathogen was identified as Sclerotinia sclerotiorum (Lib.) de Bary due to the morphology and symptoms. The detailed characteristics of the pathogen are summarised as follows. 1. Ascospores are equally binucleate and the ratio of length and width is over 2.0. Ectal excipulum at the margin of apothecium is composed of texture prismatica. 2. The size of sclerotia is approximately 2.8mm in diamter and it is usually produced at the growing margin of colony on PDA medium. Cortex of sclerotia is composed of 2-6 layers of dark browned and cycloid cells. It is composed of hyaline texure obilata within sclerotial medulla and not formed tomentum hyphae on outside of cortex. From the artificial inoculation test, 10 plant species were determined as new host plants and 2 plant species unrecorded in Korea were found.

마멸입자 형태해석에 의한 유압피스톤용 모터의 상태감시

문병주,조연상,박흥식,전태옥 韓國工作機械學會 2000 한국생산제조학회지 Vol.9 No.6

Morphological analysis of wear particles is one of useful methods for machine condition monitoring because it is well reflected in machine driving state. This paper was undertaken to apply to the condition monitoring of hydraulic piston motor. The lubricating wear test was performed under different experimental conditions using the wear test device and wear specimens of the pin on disk type was rubbed in paraffinic base oil by three kinds of lubricating materials, varying applied load, sliding distance. The four shape parameters (50% volumetric diameter, aspect, roundness and reflectivity) arc used for morphological analysis of wear particles. The results showed that the four shape parameters of wear particles depend on a kind of the lubricating materials. It was capable of calculating presumed wear volume for three kinds of mate-rials on driving time to foresee a damage condition of lubricating materials.

딸기 시들음병균에 대한 Trichoderma속 균의 길항작용에 관한 연구 5. 중복기생균 Trichoderma harzianum에 의한 딸기 시들음병의 생물적 방제

문병주,정후섭,박현철 한국식물병리학회 1995 Plant Pathology Journal Vol.11 No.4

거북밀깍지벌레(Cerostegia japonicus)의 發生消長과 그 防除에 관한 硏究

裵泰雄,曺種澤,河祥永,韓熙世,文炳周 東亞大學校 1979 東亞論叢 Vol.16 No.2

거북밀깍지벌레의 效果的인 防除法을 규명하기 위하여 釜山地域에서의 發生消長을 검토하고, 4종류의 寄主別로 기계유유제, 有機燐劑 3종 및 Carbamate劑 3종을 공시하여 殺蟲率을 Bliss의 Probit법으로 분석하고 農藥의 농도에 따른 蟲體의 呼吸代謝를 검토 하였다. 1. 釜山地方에서의 거북밀깍지벌레의 孵化器는 6月15日∼7月12日이였으며 孵化最盛期는 7月 初旬이였다. 2. 防除適期는 8月10日경이었다. 3. 8月3日에 처리한 寄主別의 본 害蟲의 LD?이 가장 낮은 것은 자목련에서는 DEP, 감나무에서는 BUX,사철나무와 치자나무에서는 BUX와 DEP로서 전체적으로 볼 때 BUX 및 DEP가 가장 效果的이고 그 다음이 Diazinon이였다. 4. 8月18日 처리에서 LD?이 가장 낮은 것은 자목련과 감나무에서는 Diazinon이, 사철나무와 치자나무에서는 BUX와 DEP로서 전체적으로 보면 Diazinon, BUX와 DEP등이 感受性이 높았다. 5. 8月3日의 처리에서 LD?이 가장 낮은 농약은 BUX로서 寄主別 농도 범위는 0.011∼0.043%였다. 6. 8月18日의 처리에서 LD?이 가장 낮은 농약은 BUX와 Diazinon으로서 寄主別 농도 범위는 각각 0.011∼0.1076%, 0.028∼0.074%였다. 7. 本 害蟲의 正常 酸素消費量은 9.25∼10.02㎕O₂/㎎ body weight/hour였으며 寄主에 따른 차이를 인정할 수 없었다. 8. Diazinon과 BUX에서는 농도가 높아질수록 산소소비량은 정상호흡량 보다 훨씬 증가하였으나 기계유유제에서는 감소되었다. In order to investigate the effective control of Florida was scale (Cerostegia japonicus), the seasonal occurrences of 1st in star in Busan area was examined, the insecticides tested with the different four hosts were carbamates(NAC,BUX BPMC), organicphosphates(DEP,MEP,Diazinon)and Machine-oil emulson, and the mortality was analyzed by the probit analysis. Also the effects of insecticide's concentration on respiratory metabolism of Florida wax scale was examined. The results obtained were as follows 1. The periods of the larva occurrence was from June 15 to July 12, and the major peak of its occurrence was found to be in the early July. 2. The adequate period for the effective control of Florida wax scale was about August 10. 3. The lowest insecticides of LD 50 values to Florida wax scale with each hosts in August 10 was DEP in the people magnolia, BUX in the persimmon tree, BUX and DEP in the spindle tree and the gardenia. So generally the most effective insecticides for controlling it were BUX and DEP, Diazinon was the next. 4. In the second experiments treated in August 18, the lowest insecticide of LD50 values to it was Diazinon in the purple magnolia and the persimmon tree, BUX and DEP in the spindle tree and the gardenia. So the susceptibility of it to the insecticides was highest in Diazinon, BUX and DEP. 5. The lowest insecticide of LD90 values to it was BUX and the concentration range by the hosts was from. 0.011% to 0.043%. 6. In the second experiment treated in August 18, the lowest insecticides of LD90 values to it were BUX and Diazinon, and the concentration ranges by the hosts were from 0.011 to 0.1076%, 0.028 to 0.074% respectively. 7. The normal oxygen consumption of Florida wax scale was from 9.25 to 10.02㎕O₂/㎎ body weight/hour and wasn't recognized the significance by the hosts. 8. The oxygen consumption of it in Diazinon and BUX was higher with increasing of insecticides concentration than the normal respiratory, but it was decreased in machine oil emulsion.

벼 담수직파재배에서 벼모썩음병에 관련된 Pythium속 균의 병원성

문병주 한국식물병리학회 1996 Plant Pathology Journal Vol.12 No.4

벼 담수직파재배에서 Pythium arrhenomanes에 의한 벼모썩음병의 생물학적 방제

문병주,박현철 한국식물병리학회 1996 Plant Pathology Journal Vol.12 No.4