老化現象

徐舞圭 서울대학교 의과대학 내과동문회 1970 含春內科 Vol.1 No.1

Etest를 이용한 피부사상균의 항진균제 감수성 검사 (초)

서무규,강교신,황성민,김동민,김재홍,하경임 대한피부과학회 2010 초록집 Vol.48 No.20

심포지엄 : 피부진균 ; 피부사상균증의 최신지견 : 투사백선

서무규 대한피부과학회 2008 초록집 Vol.46 No.20

표재성 피부칸디다증

서무규,안규중,노병인 대한의진균학회 1999 대한의진균학회지 Vol.4 No.2

Candidiasis is an acute or chronic infection caused most commonly by Candida albicans and occasionally by other species of the genus Candida. The superficial cutaneous candidiasis is varied; oral candidiasis, perieche, candidal vulvovaginitis, candidal balanitis, candidal intertrigo, erosio interdigitalis blastomycetica, perianal candidiasis, candidal paronychia, candidal onychia, chronic mucocutaneous candidiasis, congenital cutaneous candidiasis, and candidid. Diagnosis can be made by typical appearance of the clinical lesions and the presence of satellite vesicopustules. This can be confirmed by KOH examination and culture of skin scrapings. Treatment is the correction of predisposing factors, topical therapy of imidazoles and/or systemic administration of itraconazole or fluconazole. [Kor J Med Mycol 4(2): 98-103]

Acetylsalicylic Acid, Dipyridamolel, Nifedipine의 병용치료로 호전된 청피반성 혈관염 1례

서무규,윤기상,성열오 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

청피반성 혈관염은 만성 재발성 피부혈관병증으로 난치성으로 알려져 있다. 최근 혈전치료제인 acetylsalicylic acid 및 dipyridamole과 혈관확장제인 nifedipine의 병용요법으로 만족할만한 치료효과를 보였다는 보고가 있다. 이에 저자들은 19세 여자에서 임상증상, 병리조직학적 및 직접면역형광검사 소견으로 청피반성 혈관염으로 진단하고 상기 3가지 약제를 병용투여하여 병변의 호전을 보인 1예를 경험하고 문헌고찰과 함께 보고한다. We report a case of livedo vasculitis in a 19-year-old female, who showed multiple, recurrent, O.2-2.Ocm sized, erythematous punched-out ulcers on both ankles and dorsa of feet for 3 years. Histopathologic findings showed granulation tissue, hemorrhage, perivascular inflammatory cell infilteration in upper dermis. Direct immunofluorescent studies showed C3 deposit in dermal vessel wall. Therapy was undertaken with acetylsalicylic acid, dipyridamole, and nifedipine daily, resulting in almost complete resolution of the skin lesion for about 1 month after treatment.

Ribosomal DNA의 nontranscribed spacer 부위 반복요소의 증폭에 의한 Trichophyton rubrum의 균주간 동정

서무규,방장석,김정철 東國大學校醫學硏究所 2003 東國醫學 Vol.10 No.1

목적 : Trichophyton(T.) rubrum은 조갑진균증을 포함하여 피부사상균증을 일으키는 중요한 피부사상균이다. T.rubrum의 균주간 동정은 피부사상균증의 역학 및 발병기전에 대한 연구, 특히 조갑백선 치료 후 재발하는 경우 재발과 재감염을 구분할 수 있다. 최근 ribosornal DNA(rDNA)의 nontranscribed spacer(NTS)부위에 반복요소(tandemly repetitive subelements, TRSs)를 중합효소 연쇄반응으로 증폭하여 T.rubrum의 균주간의 동정이 가능하여 본 연구를 시도하였다. 재료 및 방법 : 환자에서 분리된 T. rubrum 18주와 표준 균주 1주를 포함하여 총 19주를 Sabouraud 액체배지에서 배양한 후 glass bead법으로 진균 DNA를 분리하여 진균핵내의 rDNA와 NTS 부위에 반복요소 1 및 2(TRS-1, TRS-2)를 중합요소 연쇄반응으로 증폭하였다. 결과 : TRS-1 반복 부위에 9가지와 TRS-2 반복부위에 11가지의 PCR 산물의 밴드가 나타났으며 PCR형의 조합으로 T.rubrum 19균주를 14가지형으로 구분할 수 있었다. TRS-1 반복 부위의 PCR 산물의 밴드는 PCR형 1+2형이 가장 많았고, TRS-2 반복 부위의 PCR 산물의 밴드는 PCR형 Ⅱ형이 가장 많았다. 결론 : T. rubrum의 NTS 부위의 반복 요소 1 및 2에 대한 중합효소 연쇄반응은 PCR형에 의한 T. rubrum의 균주간의 동정에 유용하였으며, 향후 T. rubrum 감염의 역학과 발병기전을 밝히는데 도움이 될 것이다. Background : Trichophyton (T.) rubrum is an important etiologic agent of dermatophytosis including onychomycosis. Although strain typing is essential for epidemiologic studies, strain typing of T. rubrum is difficult. Recently strain typing of T. rubrum using molecular method in the ribosomal DNA(rDNA) nontranscribed spacer(NTS) region have been successful. We investigated the strain typing of T. rubrum by specific amplification of tandemly repetitive subelements (TRSs) in the rDNA NTS region. Methods : 19 strains of T. rubrum(18 clinical isolates of T. rubrum and 1 strain of T. rubrum IFM 45626) were cultured on Sabouraud dextrose broth and their DNA were extracted by bead-beating method. PCR of specific amplification of TRS-1 and TRS-2 was done. Results : Specific amplification of TRS-1 and TRS-2 produced strain characteristic banding patterns(PCR types), with 9 TRS-1 and 11 TRS-2 PCR types from 19 strains of T. rubrum, and in combination, 14 separate PCR types were recognized by amplification of both TRS-1 and TRS-2. Complex pattern representing 1 plus 2 copies of TRS-1 and simple pattern showing 2 copies of TRS-2 were frequently observed among 19 strains of T. rubrum. Conclusion : The specific amplification of subrepeat element in the rDNA NTS region provided useful information for strain typing of T. rubrum.

흑색진균에 의한 감염증

서무규 東國大學校醫學硏究所 2004 東國醫學 Vol.11 No.1

흑색진균은 dematiaceous fungi라고 하며 세포막에 멜라닌 색소를 가지고 있으며 최근 국내에서 이들에 의한 피부감염이 증가되고 있어 관심의 대상이 되고 있다. 흑색진균에 의한 감염증으로는 흑색백선, 조갑진균증의 일부분, 흑색 사모증, 각막 사상균증의 일부분, 색소분아진균증, 흑색진균증, 진균종의 일부분이 포함된다. 이 중 특히 색소분아진균증은 조직에 만성육아종성 염증과 경화세포를 보이는 진균질환이며 흑색진균증은 조직에 효모양세포나 균사를 보이는 진균질환으로 현재까지 국내에 각각 9예, 6예만이 보고되어 있다. The dematiaceous fungi, characterized by having melanin in their cell walls, have become significant due to an increasing cause of human skin disease in Korea. The diseases caused by dematiaceous fungi contain pityriasis (tinea) nigra, onychomycosis (in part), black piedra, keratomycosis (in part), chromoblastomycosis, phaeohyphomycosis, and eumycotic mycetorma (in part). Chromoblastomycosis is subcutaneous mycotic disease that contain sclerotic cells and histologically show pseudoepitheliomatous hyperplasia with microabscess, and phaeohyphornycosis is a group of mycotic infections that contain dematiaceous yeast-like cells, pseudohyphae-like elements, hyphae or combination of these forms in tissue.

자가면역성 갑상선질환에서의 백반증 및 원형탈모증

서무규,이재태 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.2

We studied the association of vitiligo, alopecia areata with autoimmune thyroid disease Vitiligo, alopecia areata, vitiligo with alopecia areata were found in 21 out of 283 patients(7.4%) with autoimmune thyroid disease, 6 out of 223 patients(2.7%) with nonautoimmune thyroid disease. These results suggest stron association of vitiligo & alopecia areata, and autoimmune thyroid disease(p<0.01). The prevalence of vitiligo was similar to that of alopecia areata in autoimmune thyroid disease(3.5% vs. 3.2%). Two patients had vitiligo with alopecia areata. Witiligo in autoimmune thyroid disease was most frequently found on leg and alopecia areata in autoimmune thyroid disease was almost found on temporal area of scalp.

진균성 각막염 14예의 임상 및 균학적 관찰

서무규,성열오,윤기성,방장석,조희태,안 영,하경임 대한의진균학회 1998 대한의진균학회지 Vol.3 No.1

Background: Clinical concern and incidence of mycotic keratitis in ophthalmic practice has been increasing. Objective: The purpose of this study was to investigate clinical and mycological feature of mycotic keratitis. Methods: We evaluated the clinical and mycological aspect of mycotic keratitis in 14 patients from October 1993 to March 1997 in Dongguk Unversity Hospital. Results and Conclusion: Mycotic keratitis showed high incidence in fitth (42.9%), sixth(35.7%) and fourth decade (14.3%). The ratio of male to female patient was 1:3.6. The seasonal prevalence was highest in autumn. A scratch or abrasion from vegetation was the most common type of the eye trauma in myeotic keratitis. The positive rate of KOH examination and culture was 92.9%, respectively. The common causative organisms of mycotic keratitis were Fusarium sp. (38.5%) and Alternaria sp. (38.5%), followed by Curvularia sp. (7.7%), Aspergillus flavus (7.7%) and Acremonium sp. (7.7%). [Kor J Med Mycol 3(1): 33∼38]

Chitin Synthase 1 유전자의 염기서열 분석을 통한 피부사상균의 계통발생 및 분류

서무규,김병천,김정철 대한의진균학회 2000 대한의진균학회지 Vol.5 No.2

Background: The species of dermatophytes have been identified and classified by morphological and biochemical characterization as well as by mating experiments. But these techniques are either tiome consuming or lacking specificity. Recently molecular analysis has been introduced to the field of medical mycology. Objective: We investigated the phylogeny and taxonomy of the dermatophytes using sequence analysis of the chitin synthase 1 (CHS1) gene. Methods: 15 species of dermatophytes (6 strains of T, rubrum, 4 strains of T. mentagrophytes subtypes, M. canis, M. gypseum, E. floccosum, T. verrucosum, and T. tonsurans) were cultured on Sabouraud dextrose broth and their DNA were extracted by bead-beating method. Cloning and sequencing of PCR product were done. Results: The size of specific bands among dermatophytes was 615 bp in CHS1 gene. Phylogenetic analysis of sequences revealed that 6 strains of T. rubrum showed genetically identical pattern in intraspecies, but subtypes of T. mentagrophytes were different. The other dermatophytes showed different pattern in interspecies. Conclusion: The phylogenetic analysis of CHS1 gene provided useful information for classification and understanding the evolution of dermatophytes species. [Kor J Med Mycol 5(2): 51-59]