세균성 PROTEASE 의 정제(精製)에 관한 연구

서정훈,우두리 한국농화학회 1969 Applied Biological Chemistry (Appl Biol Chem) Vol.11 No.1

The purification methods of bacterial protease have been published by many workers, especially by the using of ion exchange resins. But in the practical application to obtain a comparatively purified enzyme, the known methods do not give always a satisfiable results. Here we developed an industrially applicable method for purification of bacterial protease with the using of tannin. By the adaptation of the optimal conditions of this method on the purification, a 150000 unit/g.(Fuld Gross unit) of protease sample could obtained.

微生物酵素에 依한 葡萄糖의 果糖轉化에 關한 硏究

서정훈,김종규,기우경,이인구,권태종,우두리 慶北大學校 生産技術硏究所 1969 生産技術 Vol.3 No.-

內容 1. 硏究題目 2. 硏究目的 및 重要性 3. 硏究方法 4. 硏究內用 및 範圍 5. 硏究結果 6. 硏究結果에 關한 實用化 方案및 建議事項 1. 硏究題目 微生物 酵素에 依한 葡萄糖의 果糖轉化에 關한 硏究 2. 硏究目的 및 重要性 現在 國內에서는 澱粉原料를 酸或은 酵素로서 糖化하여 葡萄糖或은 水飴를 製造하여 甘味料로서 使用하고 있으며 이로써 輸入糖에만 依存하였던 甘味料의 一部를 國內製品으로서 代替하고 있으며 또한 澱粉原料 生産者인 農民의 所得을 增加시키고 있다. 그러나 이 Glucose나 Syrup를 庶糖과 比較해볼 때 榮養價에 있어서는 이 兩者가 完全히 同一한 價置를 지니고 있으나 甘味에 있어서는 葡萄糖이 庶糖의 約半(6/10)밖에 되지 않으므로 特히 甘味를 要求하는 製菓類나 飮料等의 製造에는 輸入原糖에 依해 製造된 庶糖을 主로 使用하고 있다. 그러나 萬一 이 葡萄糖을 自然界에 存在하는 消化性 糖類中 가장 甘味가 높은 果糖으로 轉化시킨다면 이 轉化된 果糖과 葡萄糖의 混合物은 甘味度에 있어서나 味覺에 있어서 庶糖에 比해 조금도 損色이 없어질 것이므로 輸入糖에만 依存하고 있는 國內의 甘味料를 自家 生産함으로써 原糖輸入을 抑制하여 國內 經濟에 이바지 할 수 있을 것이며 國家經濟水準이 우리나라 보다도 더 높은 美國, 日本 等地에서도 이 點을 重視하여 硏具한 結果 1966年度부터 이 果糖 生産을 始作한 바 있으니 더구나 우리나라에서는 이의 硏究를 國家事業으로 發展시켜야만 할 程度로 重要視하여도 無妨하다고 보여진다. 3. 硏究方法 本 硏究는 實用化할 수 있는 結果를 얻을 것을 目的으로한 만큼 實驗室的으로 얻은 結果를 가지고 實用化에 對한 活用 可能 與否를 檢討할 수 있도록 硏究計劃을 確立 遂行하였다. 4. 硏究內用 및 範圍 本 硏究의 範圍는 上述한 바와같이 重要한 課題인 葡萄糖의 果糖 轉化를 國內 硏究로서 完成하여 이것을 實用化할 것을 內容으로 하였으며 그 硏究範圍는 工業化에 利用할 수 있을 程度의 酵素能을 가진 微生物을 檢索하여 그 微生物의 性質을 硏究目的에 비추어 調査硏究 하였다. 5. 硏究結果 本 硏究에서 얻어진 結果를 要約하면 다음과 같다. ① 土壤으로부터 葡萄糖轉化酵素(Glucoseisomerase)를 强하게 生成하는 菌株를 分離 하였으며 ② 이 菌의 酵素는 作用에 있어서 有害金屬을 全然要求하지 않으며, ③ 모든 酵素의 作用條件이 工業化에 適當하며, ④ 最高果糖 轉化率이 49.8%로 아주 높으며, ⑤ 高濃度의 葡萄糖 溶液에서도 아주 잘 作用한다. 以上의 結果를 現在 外國에서 利用되고 있는 條件과 比較할 때 酵素學的으로 조금도 損色이 없으며 特히 重要한 條件의 하나인 果糖生成率이 外國의 것에 比해 約 2%가 높은 成績을 얻게 되었다. 6. 硏究結果에 關한 實用化 方案및 建議事項 本 硏究는 實驗室的으로 얻어진 結果이며 이 結果를 實用化 卽 工場作業化 하는데 있어서는 實驗室的인 結果가 그대로 適用되는 面과 中間工業過程을 거쳐 再檢討 되어야 할 面이 있게된다. 이 點을 檢討해 본 結果實用化를 爲해 再檢討되어야 할 面은 菌體의 大量培養에 따르는 諸培養條件과 榮養物質을 調査하여야 할 點이 남아있는 것으로 생각되며 이 點도 現在까지의 硏究結果를 土臺로 하여 生覺할 때 實用化할 수 있는 條件의 設定이 可能할 것으로 생각된다. Contents 1. Project Title 2. Purpose and Importance 3. Methods of Research Work 4. Subject Matter and Range of Project 5. Obtained Results 6. Proposal on the Industrial Application 1. Project Title Isomerization of glucose to fructose with bacterial enzyme. 2. Purpose and Importance The glucose and syrup produced from starch material are the only substitutable materials for cane sugar. Now, in our country, the importing sugar is the only source of sweeting material for the people's every day life, and also this casts an unfavorable problems on the country's economic state. Therefore the government forced to produce starch sugar and planed a conception of reducing the amount of importing cane sugar from abroad. But on the actual problems, the consumption of glucose. These low sweetness and unpleasant taste are the remarkable defects for starch sugar consumption. On these problems, we planed to improve the sweetness and taste of starch sugar: glucose, by conversion this glucose to fructose which gives very suitable taste and sweetness. And we want to be able to substitute the glucose for cane sugar. 3. Methods of Research Work In this paper, as expected to convert glucose to fructose, the experiments were carried out mainly on the following aspects. 1) Isolation of glucose converting enzyme producing bacteria. 2) Investigation of the condition according to enzyme reaction. 3) Detection of the maximum ratio of fructose produced from glucose by the enzyme reaction. 4. Subject Matter and Range of Project It is the subject matter that the domestic research activities could bring the accomplishment of the conversion of glucose to fructose and futher more adapt these results to the industrial field. With these purposes we projected research extent as following. 1) Industrially applicable bacteria should be isolated. 2) All the enzyme reacting conditions should be investigated based on the industrial aspect. 5. Obtained Results The following results were obtained. 1) A strain of bacteria, strongly forming glucose converting enzyme, was isolated from a source of soil samples. 2) On the reaction of this bacterial enzyme, the poisonous metal compound is never required. 3) All the conditions of enzyme reaction are appropriate to the industrial application. 4) Obtained very high glucose conversion rate whose muximum fructose forming ratio was 49.8% 5) The reaction of this enzyme was considerably remarkable, and even high concentration of glucose solution. The above results, compared with the industrial method applied by foreingner's at present, are never worse at all. 6.Proposal on the Industrial Application Because the above results in this project obtained in laboratory scale, we must develop further studies on the problems of the industrial application. On this point, the nutrient requiring properties of the isolated bacteria in the enzyme forming culture are the very important on the industrial application. If we could find out economically suitable nutritional condition for the enzyme formation, the isolated bacteria, we believe, would be directly applicable in the starch sugar manufacturing field.

Wedge filtered narrow beam의 수량계측 응용예

서정훈 대한영상의학회 1971 대한영상의학회지 Vol.7 No.1

Some of the experimental radiation dose plannings for the clinical application has been developed for the treatment of the head and neck at the cancer center of Yonsei Univ. In this paper, it is fully discussed for the typical wedge field combination and with auxiliary devices such as : 1) the use of additional collimation, 2) the settings of isocenter to the wedge, 3) the parallel wedge combination, 4) the plan with the bolus and 5) arrangement of the free angle combinations.

문화기반 도시재생전략의 통합적 접근법 연구- 영국사례 중심 -

서정훈 한국지방행정학회 2015 한국지방행정학보 (KLAR) Vol.12 No.2

This article starts with a critical view about the cultural consumption-oriented urban regeneration strategies focused on the culture’s consumption and service function. Having a purpose of understanding the integrated approach of the cultural urban regeneration which regards culture as a source of creativity and innovation, the article analyses the case of the UK and draws some suggestions. Based on the analytical method reconstructed by the analysis of the preceding researches concerned with the concept and method of policy integration, this article analyses the key policy mechanism such as policy agenda・implementation・evaluation. As a result, it is found out the development of a new joint policy, the policy coherence through interdepartmental cooperation・connection・coordination, the construction of a knowledge-based system, and the formation of cooperative partnership. Under the circumstances that the role of culture in the integrated approach for urban regeneration mixed with the policy agenda of creative economy has either led the function and structure of cities under the integrated vision, or expanded the extent to social integration and community identity through admitting local cultural diversity, the knowledge-based system must be prepared for transcending cultural copy and playing role of a catalyst of creativity and innovation.

자기효능감이 블로그 운영자의 정보공유에 미치는 영향

서정훈 한국산업경제학회 2008 한국산업경제학회 정기학술발표대회 논문집 Vol.- No.춘계

[토론문] 토론문

서정훈 한국사회복지행정학회 2014 한국사회복지행정학회 학술대회 자료집 Vol.2014 No.10

삽입된 인공수정체가 혼탁하여 시행한 인공수정체 교환술의 임상결과

서정훈,형성민,Jeong Hun Seo,Sungmin Hyung 대한안과학회 2008 대한안과학회지 Vol.49 No.8

Purpose: To evaluate the outcomes of posterior chamber lens (PCL) exchange in patients with opacified foldable PCLs. Methods: This study consisted of 31 patients (35 eyes) who had received phacoemulsification and implantation of foldable intraocular lenses in the bag or sulcus and developed late opacification of the PCL. All patients reported a reduction of visual acuity and deterioration in vision. The PCLs were explanted and replaced with new PMMA lenses. The perioperative complications and the best corrected visual acuities (BCVAs) before and after surgery were evaluated. Results: The mean visual acuities (logMAR value) before and after IOL exchange were 0.59±0.80 and 0.21±0.27, respectively. The difference was statistically significant (p=0.005, paired t-test). Intraoperative complications included posterior capsule rupture in six patients, zonular dehiscence in three patients, and both in one patient. Postoperative complications included intraocular pressure elevation in five patients and cystoid macular edema in two patients. One patient showed hypopyon at 6 days postoperatively, which lasted for 3 months, but she showed good visual acuity. Conclusions: Intraocular lens exchange was a safe and effective treatment for patients whose PCLs were opacified. J Korean Ophthalmol Soc 49(8):1226-1234, 2008

산업미생물학회의 창회를 돌이켜 본다

서정훈 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1993 한국산업미생물학회 20년사 Vol.- No.-

Heterologous plasmid DNA 의 transformation에 있어서의 細胞膜 蛋白質의 기능에 對하여

徐正塤,洪淳德,李麟九,金英浩 慶北大學校 1983 論文集 Vol.36 No.-

Terramycin resistance plasmid DNA and Streptomycin resistance plasmid DNA have been prepared from Streptomyces rimosus and E. coli KPM 105, respectively, by phenol extraction of lysozyme-lysed cells. And the plasmid DNA was introduced into B. subtilis KPM 73, B. subtilis BD 224, B. subtilis 110, B. subtilis RM 125, B. amyloliquef aciens and B. megaterium IAM B425 by transformation. The variable characters affecting heterologous plasmid transformation, and the functions of competence factor and binding factor existing in the recipient cell surface were futhermore studied. Terramycin resistance plasmid was well transformed into Bacillus subtilis KPM 73 and B. subtilis RM 125, and Streptomycin resistance plasmid was well transfomed into B. subtilis BD 224, B. subtilis 110 and B. megaterium IAM B 425 at high frequency. The high frequency of plasmid transformation was obtained at 4 hr of incubationin growth medium. 20 to 50 min in competence medium, and the optimal pH and temperature for competence were 7.0 and 20C to 30C, respectively. The transformation of B. subtilis KPM 73 reached a maximum level after 20 min of exposure to DNA, B. subtilis BD 224 after 30min, and B. subtilis RM 125 after 10 min. Ca, Cu, Fe, Mg and Mn ions increased the transformation frequency of B. subtilis KPM 73 but Hg, Zn and Co ions decreased the transformation frequency. Ca ions were required for transformation of E. coli C600 and tranrsformation frequency was increased 20 times by addition of 75mM CaCl_2. The competence development of recipient cell was enhanced by addition of lysozyme in the competence medium and lysozyme was more affective in the early stage of competence development than the late stage of competence development, in which the spontaneous competence development reached maximum level. In sight of these observations, competence factor would seem to be a kind of cell wall lytic enzyme such as lysozyme. when the protoplasts of B. subtilis KPM 73 were transformed by terramycin resistance plasmid, the protoplasts were successfully transformed and the transformation frequency was 10^-2. The competent cells were treated with small amount of protein-digesting enzyme such as trypsin, and then exposed to transforming plasmid DNA. The recipient cells that were treated with trypsin were transformed 10 to 100 folds lower than non-trypsin-treated control cells. These results show that the binding factor must be a protein or at least a complex containing protein that is essential to it's activity. And external protein such as bovine albumin was effective on transformation of trypsin treated cell, suggesting that the cell surface-located binding factor did not have a specificity and the binding factor could be replaced by external substance such as bovine albumin. Terramycin resistance plasmid extracted from Ter^R transformant of B. subtilis KPM 73 was well transformed into B. subtilis KPM 73. And the electrophorsis pattern of Ter^R plasmid extracted from Ter^R transformant of B. subtilis KPM 73 was equal to that from St. rimosus.

VHL 유전자 배선 돌연변이(Q73X)를 동반한 제1형 von Hippel-Lindau병 1예

서정훈,양재홍,최평락,김유리,최영식,박요한,고지호,강효정,김일진,박재갑 대한내분비학회 2006 Endocrinology and metabolism Vol.21 No.3

Von Hippel-Lindau (VHL) disease is an autosomal dominant neoplasia syndrome that result from a germline mutation in the VHL gene. Germline mutation in the VHL gene lead to the development of hemangioblastomas of the central nervous system and retina, cysts and clear cell carcinoma of the kidney, cyst adenomas of other organs, and pheochromocytoma. VHL is a tumor suppressor gene on the short arm of chromosome 3. VHL disease has been classified into two main clinical subtypes depending on the presence (type 2) or absence (type 1) of pheochromocytoma. Type 2 has been subdivided into three categories depending on the presence (type 2B) or absence (type 2A) of renal cell carcinoma, with type 2C being a rare subtype in which pheochromocytoma is the sole manifestation of VHL disease. Recently we experienced a family with VHL type 1 who carry C to T (Q73X) transition in codon 217 nonsense germline mutation in exon 1 of VHL gene. The authors report this case with literature review. (J Kor Soc Endocrinol 21:239~244, 2006) Von Hippel-Lindau (VHL)병은 VHL유전자의 배선 돌연변이(germline mutation)의 결과로 발생하며, 상염색체 우성으로 유전하는 질환으로 소뇌, 척수, 뇌간의 혈관모세포종과 망막의 혈관종, 신세포암, 갈색세포종 및 췌도종양 등의 여러 장기에 양성 및 악성 종양을 초래하는 질환이다. 저자들은 췌장과 신장에 종양을 가진 환자와 소뇌의 종양을 가진 딸에서 시행한 유전자 검사상 VHL exon1의 codon 217에서 C→T (Q73X)로 무의미돌연변이를 경험하였기에 문헌고찰과 함께 보고하는 바이다.