한국산 호도의 단백질 및 지질의 조성에 관한 연구

최청,성태수,차원섭,손규목 한국농화학회 1986 Applied Biological Chemistry (Appl Biol Chem) Vol.29 No.3

We employed gel filtration, polyacrylamide gel electrophoresis, amino acid autoanalyzer, thin layer chromatography for determining protein and lipid composition in walnut. The walnut contained 22.18% of crude protein and 64.23% of crude lipid. Glutamic acid (38.60%) was the major amino acid in soluble protein, followed by arginine and aspartic acid. The sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis showed 12 band in soluble protein of walnut, and collection rate of main protein fraction purified by Sephadex G-150 was 60.67%. The molecular weight for t he main protein was estimated to be 43,000. The lipid fraction obtained by silicic acid column chromatography were mainly composed of about 93.05% neutral lipid, whereas compound lipid was only 7.0% level. Among the neutral lipid by thin layer chromatography, triglyceride was 82.05%, sterol ester and free fatty acid were 3.86% and 4.80%, respectively. The predominant fatty acids of total and neutral lipids were linoleic acid (64.48∼69.98%) and oleic acid (13.89∼15.36%). The major fatty acids of triglyceride separated from neutral lipid were linolenic acid (69.98%).

대학입시와 시장 기능

최청일 대한사립학교장회(구 대한사립중고등학교장회) 1998 私學 Vol.83 No.-

고상법에 의한 Bradykinin 유사물의 합성 (II)

최청,이재성,배만종,윤상홍,Choi, Cheong,Lee, Jae-Sung,Bae, Man-Jong,Yoon, Sang-Hong 생화학분자생물학회 1982 한국생화학회지 Vol.15 No.4

Bradykinin(BK)의 receptor $B_1$ 상에서 nonapeptide의 구조 및 활성에 대한 연구 목적으로 bradykinin유도체 ($Leu^1$, $Leu^2$)BK, ($Leu^2$, $Leu^3$)BK, ($Ala^7$, $Ala^9$)BK 및 ($Ala^8$, $Ala^9$)BK을 고상법으로 합성하였다. coupling은 N,N'-dicyclohexylcarbodiimide로 행하였으며 HBr응액으로 cleavage한 후 peptides는 Sephadex G-25와 carboxymethyl cellulose칼럼 크로마토 그라피로 정제하였다. 이들 peptides의 순도측청은 paper chromatography, thin layer chromatography, paper electrophoresis, slab gel electrophoresis. melting point 및 아미노산 분석에 따랐다. endopeptidase인 ${\alpha}$-chymotrypsin과 trypsin, exopeptidase인 carboxypeptidase A와 leucine aminopeptidase를 사용하여 이들 peptides의 분해실험을 하였다. 즉 carboxypeptidase A와 ${\alpha}$-chymotrypsin은 이들을 빠르게 분해하였고 trypsin은 느리게 분해 하였으나, ($Ala^7$, $Ala^9$) 및 ($Ala_8$, $Ala^9$)BK은 N-말단에 모두 proline의 imino와 결합되었기 때문에 leucine aminopeptidase의 작응을 받지 않았고 ($Leu^1$, $Leu^2$)BK은 1번 위치에 arginine과 같은 방향족 아미노산이 치환되지 않았으므로 trypsin의 작응을 받지 않았다. We have synthesized, by solid phase method, a series of 4 analogues of the nonapeptide, bradykinin(BK) in order to conduct a structure-activity study of these peptides on the newly discovered bradykinin $B_1$ receptor. Coupling were performed by dicyclohexylcarbodimide. After cleavage with liquid HBr, peptides were purified by gel fitration on Sephadex G-25 and ion-exchange chromatography on carboxymethyl cellulose. The purity of the nonapeptide was then checked by paper chromatography, thin layer chromatography, paper electrophresis slab gel electrophoresis, melting point and amino acid analysis. ($Leu^1$, $Leu^2$), ($Leu^2$, $Leu^3$), ($Leu^7$, $Leu^9$) and ($Leu^8$, $Leu^9$) analogues of bradykinin were incubated in vitro with endopeptidase ($\alpha$-chymotrypsin, trypsin) and exopeptidase (carboxypeptidase A, leucine aminopeptidase) in order to study the degradation patterns of peptides. ($Leu^1$, $Leu^2$), ($Leu^2$, $Leu^3$), ($Leu^7$, $Leu^9$) and ($Leu^8$, $Leu^9$) analogues of bradykinin were rapidly degraded by $\alpha$-chymotrypsin and carboxypeptidase A, while the degradation by trypsin was slow. But ($Ala^7$, $Ala^9$) BK and ($Ala^8$, $Ala^9$)BK contain imino peptide bond from proline at N-terminal and therefore they were not attacked by leucine aminopeptidase.

동결보호제로서 미성숙 송화추출물이 Lactococcus lactis에 미치는 영향

김성,배만종,최청 경산대학교 생명자원개발연구소 2000 생명자원과 산업 Vol.4 No.-

송화추출물 첨가가 Lactococcus lactis 성장에 미치는 영향을 알아보기 위하여 Elliker broth에 1, 3, 5% 첨가한 결과, 3% 첨가에 의해 균성장이 뚜렷이 촉진됨을 알 수 있었다. 동결건조컬쳐 제조시 중화제로 사용되는 Na citrate의 적절한 첨가 농도를 알아보기 위하여 도결건조컬쳐에 Ls. lactis를 1% 접종한 후 Na citrate를 각각 1.5%와 2% 첨가하ㅕ 배양하면서 생균수를 측정한 결과 2% 첨가에 의해 생균수가 가장 높게 나타났다. 또한 동결건조 컬쳐의 세포 활력을 산 생성능으로 측정한 결과, 3% 송화추출물 첨가구의 활력은 76%, β-glycerophosphate 첨가구는 73%, sucrose와 gelatin 첨가구는 각각 69, 68%를 나타내어 송화추출물 첨가구의 활력이 가장 높게 나타나 송화추출물이 동결건조컬쳐 제조시 세포의 효소를 잘 보호하여 산 생산에 우수성을 보이는 것을 알 수 있었다. 동결건조컬쳐 제조후 적당한 저장조건과 온도를 알아보기 위하여 산소와 질소 가스 하에서 -15℃와 25℃로 각각 저장한 후 생균수와 산도를 측정한 결과, 동결보호제 송화추출물을 첨가하여 질소 가스주입하에서 -15? 저장하는 것이 생균수의 가장 높게 나타났고 세포 활력 유지에 가장 적당한 것으로 나타났다. This studies was carried out to abserve the growth and the activity Lactococcus lactis with immature pine pollen extract(IPPE) in Elliker broth, skim milk and the effection of IPPE as cryogenic compound in Lyophilized culture. Growth and activity of Lactococcus lactis Elliker broth with IPPE were better than control at 28? for 20 hrs. Survival rate and activity of Lactococcus lactis freeze dried culture added IPPE as cryogenic compound were higher than control, when the lyophilized culture were tested for survival rate and acid producing activity after storage for three month by rehydration with skim milk to original prelyophilization volum. Survival rate and activity of Lactococcus lactis freeze dried culture sealed with N ₂ gas were higher than O₂ gas, when the lyophilized culture were tested for survival rate and acid producing activity after storage for three month by rehydration with skim milk to original prelyophilization volum. Survival rate and activity of Lactococcus lactis freeze dried culture stored under -15℃ were higher than 25℃, when the lyophilized culture were tested for survival rate and acid producing activity after storage for three month by rehydration with skim milk to original prelyophilization volum.

敎師의 給與政策 比較硏究

최청일 東亞大學校 學生生活硏究所 1998 學生硏究 Vol.17 No.-

The purpose of this study was to review teachers' salary policy in the United States. The present study gave attention to personnel policies that bore on school financing and provided specific detail and discussion of teachers' salaries. Of concern was the relative financial condition of teachers in the national economy. Teacher policies that addressed both the needs of the children and the welfare of the teachers were necessary for a productive educational system. Such personnel policies were affected by the supply of a quality teaching force as well as by the economic and demographic conditions of the school district. Collective barganining played an important role in the welfare of teachers in over half of the states : but, the evidence was inconclusive as to whether over the long term, teachers' salaries were higher in the states and school districts where collective bargaining was practiced. Teachers had historically been subjected to low levels of compensation. Adam Smith attributed the low wages of teachers to the plentiful supply. The fact that public school teaching was not subject to conventional market forces with regard to both supply and demand prevented teachers' salaries from being competitive with other white-collar employments. Rather than having the characteristics of an open marketplace the public school teaching profession exited as an oligopoly wherein the state legislature could if it chose control supply and demand. The measurement of both the supply and demand of teachers was extremely complex. The supply was generally dependent on the number of teachers continuing in the profession and the number of new entrants. Both variables were dependent on facors such as age of teachers, retention power of the profession, opportunities for other employment, and the like. Further complication was added by the length and number of career breaks and the gender of teachers. Investment in public schools and the economic conditions of teachers was influenced by the general condition of the economy. In periods of revenue shortfalls, teaches tended to personally absorb or cushion the effects of the economy. An objective measurement of how much teachers should be paid to provide a quality education was not likely to be found. If teachers' salaries exceed the rise of inflation it Is thought to be good. If teacher’s salaries in the United States were relatively better than in other countries, there might be reason to assume some relative elevation of teaching status in the economic system. Today, teachers' salaries fall short of most other white-collar prolessions in the United States, but appear to provide greater purchasing power for U.S. teachers than teachers' salaries in other advanced nations.

사학재정의 정책방향

최청일,고영진 東亞大學校 學生生活硏究所 1995 學生硏究 Vol.17 No.-

대학재정, 어떻게 운영(관리, 통제)할 것인가?

최청일 한국교육행정학회 1992 敎育行政學硏究 Vol.9 No.2

2003학년도 재학생 실태조사

최청일,박자연,김주현 東亞大學校 學生生活硏究所 2004 學生硏究 Vol.32 No.-

고상법에 의한 Angiotensin Ⅱ 유사물의 합성(제2보)

崔淸 嶺南大學校附設 基礎科學硏究所 1982 基礎科學硏究 Vol.2 No.-

Synthesis of (5-Gly), (6-Gly) and (8-Gly) analogues of angiotensin Ⅱ by solid phase method using an improved reaction vessel was carried out. I employed gel chromatography, paper chromatograpy, thin layer chromatography, paper electrophoresis, salb-gel electrophoresis and amino acid analysis for purification and for determining the purity of synthesized peptides. (5-Gly), (6-Gly), (7-Gly) and (8-Gly) analogues of angiotensin Ⅱ were incubated in vitro with endopeptidase (α-chymotrypsin, trypsin) and exopeptidase (carboxypeptidase A, leucineamineaminopeptidase) in order to study the degradation patterns of the peptides. (5-Gly), (6-Gly), (7-Gly) and (8-Gly) analogues of angiotensin Ⅱ were rapidly degradated by α-chymotrypsin, trypsin, and carboxypeptidase A while the degradation by leucineaminopeptidase is slow. (7-Gly) angiotensin Ⅱ contain imino position and therefore it was not attacked by leucineaminopeptidase.

부동산공시제도의 개선방안에 관한 연구

최청인 한국지적학회 2002 한국지적학회지 Vol.18 No.2

우리나라의 부동산공시제도는 지적과 등기로 이원화되어 있고, 부동산제도는 토지와 건물로 분리되어 있다. 이들 공부의 등록 내용과 운영 실태를 분석한 결과 행정력 낭비와 민원 처리의 번잡을 초래하고 있다. 이의 개선은 지적과 등기를 단계별로 정보연합, 공부통합, 조직통합 방식으로 추진하는 방안을 마련하고, 토지, 건물의 등록관리 일원화와 건축물 부지의 토지합병정리를 선결과제로 제시하였다.