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시판 청국장중에 함유된 Bacillus subtilis 균 포자의 가열살균 특성
최경호,조미영 대구효성가톨릭대학교 응용과학연구소 1997 응용과학연구논문집 Vol.5 No.-
Thermal sterilizing characteristics of the bacterial spores in Chunkukjang were investigated to control the number of viable spores without distingushable browning of Chungkukjang. A commercially available Chungkukjang(Pulmuwon Co.), contained a large number(1.63×10 exp (9)cells/g. wt.) of microorganisms. Bacterium was the only microorganism detected from the sample. Majority of the bacteria were spore forming, Gram positive, aerobic bacteria. The organism was a strain of Bacillus subtilis. Coli form bacterium was not detected, while a small number of Gram positive, spore forming, anaerobic bacilli, Clostridia, were found from the sample. Bacterial spores occupied 77% of the total cell number. Viable number of the spores showed a logarithmic decrease by autoclaving. However, repeated sterilization at 100℃ with 24 hours of interval was not effective. D-value at 115℃ was 2.1∼2.2 minutes. It was estimated from the value that number of viable spores could be reduced near to 1.0×10 exp (3)cells/g sample by autoclaving for 10 minutes at 115℃. When the samples were atoclaved for 10 minutes at 115℃, 1.54×10 exp (3) spores/g sample were viable.
최경호 전주대학교 자연과학종합연구소 1996 전주대학교 자연과학연구소 학술논문집 Vol.9 No.1
Greenberg et al.(1971) Suggest an extension of the randomized response technique to quantitative characters. Later, new technique is suggested by Dalenius and Vitale(1974) and much work has been reported in the literature on the estimation of c.d.f's and densities(Duffy and Waterton(1984)). In this paper, we studied two stage randomized response technique to quantitative character and compared efficiency with another technique.
최경호 전주대학교 산업경영연구소 1996 産經論叢 Vol.13 No.-
확률화응답기법(randomized response techique)이란 민감한 사안에 대한 조사시 무응답이나 거짓으로 인한 비표본오차(non-sampling error)를 줄이기 위하여 Warner(1965)에 의해서 제안된 간접조사기법이다. 그런데 확률응답기법을 이용한 조사시 우리가 간과해서는 안될 중요한 사항중의 하나는 응답자로부터 많은 정보를 얻어내려고 하다보면 응답자의 신분보호(privacy protection)정도가 떨어지게 된다는 점이다. 이에 본 논문에서는 부차정보(sub-information)를 알 때 이를 이용한 새로운 확률화응답기법을 제안하고, 제안된 기법이 Warner기법에 비하여 효율적일 수 있는 조건과, 또 신분보호정도가 같게 되는 조건을 찾아 확률화응답기법을 신분보호측면에서 고찰해 보고자 한다.
Red光 處理에 의한 麥芽의 α-Amylase 生合成 誘導와 電子顯微鏡的 考察
崔慶浩,金順東,崔奉順 대구효성가톨릭대학교 1983 연구논문집 Vol.26 No.1
六條大麥의 發芳中 α-amylase 生合成에 미치는 赤色光 및 靑色光의 效果를 檢討한 結果는 다음과 같다. 1. 보리發芽 중 α-amylase 活性은 5일째 가장 높았으며, 이때의 α-amylase 活性은 暗所 880, 靑色光 1268 unit(degradated starch ㎕/min/g fresh weight)로 赤色光處理區에서 가장 높았다. 2. α-Amylase 의 比活性은 暗所 7.4 靑色光 7.8 unit 로 有意的인 差異가 없었으나 赤色光處理區에서는 11.0 unit/mg protein으로 對照區인 暗所보다 44%가 增加되었다. 3. 赤色光處理에 의한 α-amylase의 活性은 100Lux로 1日 1回 3時間 處理한 것이 가장 높았다. 4. 5日間 栽培한 보리의 蛋白質을 電氣泳動한 結果 5個의 band가 分離되었으며 α-amylase 의 band는 赤色光處理가 暗所의 比하여 더욱 뚜렷하였다. 5. Aleurone 層과 endosperm 部는 發芽 2日부터 膨潤하기 시작하였고 aleurone 層에 인접된 澱粉粒子부터 溶解되었으며, 溶解程度는 赤色光處理區에서 더욱 促進되었다. 6. Aleurone cell wall은 發芽其間에 따라 膨潤度가 높아졌으며 赤色光處理區에서 顯著하였다. 그리고 暗所에 比하여 崩壤現象이 促進되었다. This experiment was designed to understand the effect of red light irradation on it in the α-Amylase biosynthesis during barley germination. Light treatment was done at 18℃, 50~150Lux for 1~5 hours a day under blue and red filter during germination periods. α-Amylase activity showed the highest degree at 5 days on germination. In this case, α-Amylase was 880.0 unit darkness, 970.4 unit under blue light and 1267.5 unit under red light. The specific activity of α-Amylase was 7.4 unit in darkness, 7.8 unit under blus light and 11.0 unit under red light showing 44% increase compared with the activity in the darkness. α-Amylase activity under red light reached its peak when the malt was placed under 100Lux for three hours a day. The malt protein were separated into five bands by electrophoresis, and α-Amylase band was the more prominent under red light than the darkness. Aleurone layer and endosperm began to expand on the second day of the germination period, and solubilization of starch began to occcur in the vicinity of aleurone layer. Starch solubilization in endosperm was more prompted under red light. Swelling of aleurone cell wall observed by electromicroscope became greater than darkness, and breaking state of cell wall was more prompted under red light. Swelling of aleurone cell wall observed by electromicroscope became greater than darkness, and breaking state of cell wall was observed considerable at last period of germination under red light.
Clostidium sporogenes의 原形質 Adenosine triphosphatase의 分離 및 精製
金景和,崔慶浩 대구효성가톨릭대학교 1987 연구논문집 Vol.34 No.1
對數增植 中期의 C. sporogenes는 乾操菌體 g당 29.7mg의 DAN와 320mg의 蛋白質을 含有하였으며 菌體를 超音波로 破碎하였을 때 約 90%의 DAN와 70%의 蛋白質이 遠心上澄液 劃分에 含有되었다. 菌體는 5.12μmol Pi/mg dry|cell/min의ATPase 活性을 나타내었으며 그중 約 73%의 活性은 原刑質내에 遊離된 狀態로 存在하고 約9%의 活性이 原刑質膜에서 檢出되었다. 原刑質內의 遊離 ATPase를 SephadexG-75로 column chromatography한 結果 31번과 51번 fraction으로 分離된 2個의 活性 peak를 나타내었으며 31번 peak는 蛋白質peak와 一致하였다. 그중 51번 fraction 電定永動하여 單一 band를 確認하였다. 精製한 酵素液 中의 ATPase 活性은 135.3μmol Pi/ml/ min이었고 蛋白質에 對한 比活性은 287.9μmol Pi/mg protein/min로 각각 超音波 上澄液의 16培에 達하였으나 酵素의 最終收率은 19.5% 不過하였다. Actively growing cells of Clostridium sporogenes(IFO 12636) contained 29.7mg of deoxyribonucleic acid(DNA) and 320mg of protein per gram dry cell. They exhibited an activity of adenosine triphosphatase(ATPase) releasing 5.12μmol Pi per mg dry cell per minutes. Supernatant fluid of the cells disruptured by sonication contained more than 90 precent of DNA and 70 percent of protein. The fluid showed 73 percent of ATPase activity of whole cell. When the fluid was dialysed and dluted through Sephadex G-75 column, the enzyme activity formed separated in to two peaks near the fractions of No.31 and 51, while protein formed single peak near the former fraction. The later fraction formed single protein band on polyacrylamide gel by electrophoresis, however, the former fraction formed several band. Lysate of purified ATPase released 135.3 μmol Pi per ml per minutes and showed a specific activity of 287.9μmol Pi per mg protein per minutes. It was estimated that ATPase activity increased for 16 folds of initial fluid by the purification, and that final yield of the enzyme was 19.5 percent.
LPG 액정분사식 대형 버스용 엔진 피스톤의 피로수명 해석과 냉각조건 평가
최경호,이부윤 한국마린엔지니어링학회 2004 한국마린엔지니어링학회지 Vol.28 No.5
Fatigue life of a Piston for large liquid Petroleum liquid injection(LPLi) bus engines is analyzed considering effects of cooling condition parameters : temperature of cooling water, and heat transfer coefficients at oil gallery and bottom surface of piston head. Temperature of the piston is analyzed with varying cooling conditions Stresses of the piston from two load cases of pressure loading. and pressure and thermal loading are analyzed Fatigue life under repeated peak pressure and thermal cycle is analyzed by the strain-life theory. For the two load cases, required loading cycles for engine life are defined, and loading cycles to failure and partial damages are calculated. Based on the resulting accumulated fatigue usage factors, endurance of the piston is evaluated and effects of varying cooling condition Parameters are discussed.