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Baculovirus Vector System에 의해 발현된 재조합 Pseudorabies Virus Major Capsid Protein의 면역원성
전무형,안동준,장경수,조용성,박종현,송재영,현방훈,안수환 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-
The recombinant pseudorabies virus major capsid protein (rMCP) was produced by expression of the MCP gene in Sf-9 cell using baculovirus transfer vector system. Following evaluation of the immunochemical properties of the rMCP, the immunogenicity of the recombinant subunit protiens were investigated in guinea pig and swine to obtain the preliminary guide line for the subunit vaccine using rMCP and gP50. It was proved that ultrasonication and 30% ammonium sulfate was most efficient to concentrate and purify the protein. The rMCP was safe in mice, guinea pigs and piglets. In guinea pigs, rMCP mixed with various adjuvants induced substantial degree of serum neutralizing antibody titers, but revealed incomplete protecivity against challenge. In swine, the combination of rMCP and gP50 showed the higher serum neutralizing antibody titers and cellular immune responses than rMCP alone. However, the protectivity was lower in comparison with the commercial gI-deleted inactivated vaccine. We expect these results to contribute to characterization of MCP gene of Korean isolate of PRV and to ultilize as preliminary information for prodution and evaluation of PRV recombinant subunit vaccines.
國內에서 分離한 Aujeszky's Disease Virus의 家兎에 대한 病原性
全茂炯,趙聲煥,金敎準,朴性國,鄭炳鐸 충남대학교 농업과학연구소 1988 農業技術硏究報告 Vol.15 No.-
To evaluate pathogenicity in rabbits for Aujeszky's disease virus, NYJ-1 strain, isolated from pigs in Korea, the virus at 10^5.7 TCID_50/0.2㎖ was inoculated subcutaneously to rabbits, and virological and histopathological studies were carried out. All of the rabbits infected with 0.25㎖, 0.5㎖, l.0㎖ and 2.0㎖ of virus at 10^5.7TCID_50/0.2㎖ were died at 36 to 72 hours post-infection, manifesting the clinical signs of depression, frensy, scratching, biting and pruritus. The virus was recovered from the tissues of tonsil, spleen, brain, lung, liver, kidney and blood of the succumbed rabbits, showing the highest virus titers of 10^3.5 to 10^5.6TCID_50/0.2㎖ in tonsil and comparatively lower virus titers of 10^2.4 to 10^3.5TCID_50/0.2㎖ in brain. At autopsy no specific lesions were observed in parenchymal organs. By histopathological studies. congestion, hemorrhage and necrosis were recognized in the tissues of tonsil, liver, spleen, lung, kidney, adrenal gland and jejunum. Intranuclear inclusion body was observed in the epithelial cells of tonsil and jejunum.
전무형,이헌준,박정우,안수환 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-
Aujeszky's disease virus(ADV), NYJ-1 strain, isolated from the diseased piglets in Korea was inactivated with binary ethyleneimine or formalin and absorbed in aluminum hydroxide gel. Safety and humoral and cellular immunogenicity of the antigens were investigated in mouse, guinea pig and piglets. In mouse inoculation test, the antigens showed 100% of safety and induced a significant level of ADV antibody. The protective potency following intracerebral challenge with the virulent ADV at 10^7.5TCID_50/0.2㎖ were 60% to 64.3%. In guinea pig inoculation test, it was found that the antigens were quite safe, inducing 63.2 to 124.9 of virus neutralization(VN) titers at the 5th week post inoculation(pi). The protectivity of the antigens against the challenge of the virulent ADV were 62.5% to 75.0%. In piglet inoculation test, the antigens were quite safe after the primary and booster injections, and induced 28.7 to 39.1 of VN titers at the 5th week pi. The high levels of ADV antibodies were also recognized by agar gel precipitation test, latex agglutination test and immunoenzyme assays. Leucocyte adherence inhibition(LAI) test for the piglets revealed 22.3±3.8% to 32.8±6.9% of LAI index at the 4th week pi, and the evident reactions of delayed type hypersensitivity was observed in subcutaneous inoculation of the antigen to the immunized piglets. Following the challenge, the immunized piglets showed none of specific clinical signs of AD and revealed the higher gain of body weight, and the lower secretion of ADV from the nasal cavity as compared with the control group.