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- 저자 : 中野 幸二 (검색결과 1 건)
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Development of Surface Plasmon Resonance Immunosensor for the Determination of Methyl Parathion
田中 真,由美,阪本 一平,中嶋 秀,宗 伸明,中野 幸二,CHUNG, Duck-Hwa,今任 稔彦 The Japan Society for Analytical Chemistry 2007 分析化學 Vol.56 No.9
<P>A surface plasmon resonance (SPR) sensor for the determination of methyl parathion (MP) was developed using an indirect competitive immunoassay. A sensor chip was prepared by immobilizing a MP-BSA conjugate on a gold film on the sensor chip, followed by blocking with BSA to prevent the non-specific adsorption of MP and an anti-MP antibody onto the unmodified surface of the sensor chip. An evaluation of the adsorption equilibrium of MP-BSA onto the sensor chip by measuring the SPR angle shift revealed that the adsorption equilibrium was expressed by a Langmuir-type adsorption isotherm equation. The adsorption constant and the adsorbed amount of MP-BSA onto the sensor chip were found to be 5.0 × 10<SUP>5</SUP> M<SUP>−1</SUP> and 0.95 ng/mm<SUP>2</SUP>, respectively. An MP solution (1∼5000 ppb) containing 60 ppm anti-MP antibody was introduced into the SPR sensor chip, and then the SPR angle shift was measured. The detection limit, defined as the angle shift of 85% for the blank, was found to be 10 ppb. The anti-MP antibody bound to the MP-BSA conjugate on the sensor chip was able to be dissociated by introducing an HCl-glycine solution (pH 2) into the sensor chip. Duplicate measurements with a single sensor chip were possible for at least 20 times.</P>
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