밤나무 혹벌의 寄生蜂인 남색꼬리좀벌 Torymus beneficus Y. 에 관한 硏究

李海浜,金鎭澤 동국대학교 농림과학연구소 1979 農林科學 論文集 Vol.5 No.-

From 1977 to 1978. the distribution and their parasitism of parasitoid Hymenoptera, Torymus beneficus Y. were studied from ten areas in Korea. The results are summarized as fallows; 1. Torymus beneficus Y. were distibuted in all studied areas. 2. Parasitism (%) of Torymus on Detocosmus had a big difference depend on collection sites from 65.96 % in Seoul to 0.4% in Kwangju. 3. On number of Dry ocosmus per gall, there were no difference between dense plantation area and disperse plantation of chestnut tree. but parasitism of Torymus were difference between two sites from 13.91 % in disperse plantation area to 40.53% in dense plantation area.

아황산가스가 집파리의 산란시기와 우화율에 미치는 영향

이해풍,이복임,최덕일 동국대학교 산업기술환경대학원 1994 산업기술논총 Vol.2 No.-

When Musca domestica L. was exposed to the different levels of SO_2(0.3, 0.4, 1.5 ppm) in an air pollutant fumigation chamber, in the case of 0.4 ppm exposed, their oviposition rate significantly decreased with the increasing exposure period at 0.4 ppm and their oviposition was also delayed. The number of pupae from the house fly exposed to SO_2 distinctly decreased when compared with control group and this trend was more pronounced at F_2 than at F_1 generation. The emergence of pupae from the cohorts exposed to SO_2 showed a distinctly decreasing trend with the increase in exposure period and in the concentration of SO_2.

메기 Parasilurus asotus L. 췌장의 췌도세포에 관한 세포화학적 연구

李海浜,金鎭澤 동국대학교 1986 論文集 Vol.25 No.-

Pancreatic islet cells of Catfish Parasilurus asotus L. were observed under light microscope. The results obtained were as follows; 1. the pancreas of Catfish consists of thronging Parenchymal tissue and pancreatic islets were located in the exocrine portion of the pancreas. 2. The pancreatic islets were surrounded by distinctive connective tissue capsul with the same morphological characteristics as principal islets of other teleosts. 3. The pancreatic islet was composed of B, A_1, A_2 cells. 4. AF-positive B cells were located in the central portion of the pancreatic islet and A_1, A_2 cells in the periphery of the pancreatic islet. 5. A_1 cells have the long cytoplasmic processes.

논에 殺蟲劑 처리가 天敵昆蟲과 거미群集에 미치는 영향

李海浜,金胄弼,全正蘭 동국대학교 산업기술대학원 1993 산업기술논문집 Vol.1 No.-

An arthropod community structure in a rice peddy field ecocystem was investigated. Sampling was conducted using D-vac sucker at the Experimental Station of Dongguk University in Namyangju-Gun, Kyonggi province during the periods from August to October in 1992. During the period of investigation, 112 species, 34 families and 6 orders were represented for the insect cummunity in untreated plots, while 104 species, 41 families and 5 orders in treated plots. And among them, 45 and 31 species of entomophagous insect were listed from the untreated and treated plots with 17.5% vs 5.2% in the occupancy rate, respectively, suggesting that pesticide usage lead the reduced number of immigrating and emigrating natural enemies. The other hand, 25 species in 13 families of the spider were represented in the untreated plots whereas 15 species in 8 families in treated plots. Spiders which collected in untreated plots were twice as high in abundance as those in treated plots. Tetragnatha sp. (Tetragnathidae) was the most abundance species in treated plots. Dominant species of untreated plots, Piratasubpiraticus (Lycosidae), occurred in very low number in treated plots. During the growing season, the temporal pattern of species diversity are measured using species diversity (H'), species richness (S) and species evenness index (J'). The tendency of communities to return to its predisturbed state were observed in treated plots. The positive correlation was obtained for spider species diversity and prey abundance in untreated plots but in treated plots.

산림토양내의 병, 해충방제를 위한 생태학적 기초연구 : I.소나무림과 활엽수림내의 지표곤충 1.Soil Surface Insects in Pine Plantation and Oak Forest

이해풍,이건형,김상훈 東國大學校 1989 論文集 Vol.28 No.-

본 연구는 경기도 남양주군 조안면 진중리 소재 동국대학교 연습림의 소나무림과 참나무림 내에서 1987년 5월부터 11월까지 실시되었으며 지표토양곤충군집을 대상으로 종의 구성, 먹이식습성의 구성, 우점종의 구성, 균등도 등이 조사되었다. 조사된 종은 소나무림 내에서 41과 94속 128종으로 확인되었으며 종의 수가 많은 것으로는 딱정벌레목, 벌목, 톡톡히목 그리고 파리목이었고 참나무림의 경우는 46과 95속 135종이었으며 딱정벌레목, 벌목, 톡톡히목 그리고 파리목 순으로 종수가 많았다. 식습성에 따른 영양관계구성은 초식성의 것이 두 지역 모두 가장 많아 소나무팀에서는 전체 절지동물중 43.6%이었고 다음으로 검류를 포함한 포식충류가 37.2% 그리고 잡식성인 것이 19.0%이었다. 한편 참나무림에서는 각각 36.8%, 47.8% 그리고 15.3%를 나타내어 전체적으로는 종의 다양도에서 소나무림이 약간 높았을 뿐 뚜렷한 차이를 볼 수 없었다. 계절별 군집의 변화동태에서는 두 지역간 약간의 차이를 보여서 포식자의 발생밀도가 참나무림에서 9월 중순에 대조적으로 높았을 뿐 큰 차이없이 전체적으로 5-7월에 높았고 9-10월에 낮았다. During the period from May, 1987, to November, 1987, (on the basis of pitfall trap capture,) 128 species and 135 species of insects together with unidentified were collected in soil surface of Pine plantation and Oak forest respectively in Kyounggi-Do. Spiders were most abundant species group, comprising 22.6% of the total number of individuals in Pine plantation. the most abundant insect groups were Coleoptera, Hymenoptera, Collembola and Diptera in Pine plantation, and Coleopteram Hymenoptera and Diptera in Oak forest. Oak forest supported more species than Pine plantation which supported only 4 species at very high population levels and the rest at low levels. Many of the species in two study areas were either general plant feeders or predators. Seasonal abundance of the total insects including spiders was high in the periods of May to July and low in Nomber.

누에의 行動半徑에 對한 系統間 差異에 관한 硏究

朴年圭,李海浜,金惠英 동국대학교 자연과학연구소 1986 자연과학연구 논문집 Vol.6 No.-

우리나라 장려 잠품종의 원종 중에서 14품종을 공시하여 주에의 행동반경에 대한 차이와 유전력 및 실용형질과의 관련성을 분석하였다. 행동반응은 유충의 각 시기에 따라서 품종간 차이가 있었으며 대체로 일본계통이 중국 계통에 비하여 민감한 경향이었고 각 품종 공히 행동반응이 가장 민감한 시기는 숙잡기였으며 다음은 개미누에였고 2령부토 5령까지는 상당히 둔한 경향이였다. 행동반경의 추정된 유전력은 유충의 시기별로 차이가 있었으며 h²(%)=52,121~88,411의 범위였으며, 행동발경과 타협질과의 관계에 있어서 행동반경과 생견 1 ㅣ 과수 및 행동반경과 최청부터 발아까지의 기간 사이에서는 "正"의 상관, 행동반경과 고치무게의 시이에서는 "負"의 상관이 인정되었다. The strain differences in larval walking behavior and their heredity in 14 varieties fo silk worm, Bombyx mori, has been investigate. The strain difference of walking response have been responsed in each larval stage and the bahavioral response of Japanse races was more sensitive than that of Chinese races, mostly. During matured silk worm larval stage the walking response was the highest and the next was the larvae hatched newly. Also very low sensitivity appeared from 2nd instar to 5th instar. Heritability of larvar walking distance were different depend on the larval instar the range of heritabilites(%) was 52.121-88.411. There was positive corelation between walking distance and period from incubation to emergence, but negative co relation between walking distance and single cocoon weight.

긴볼레기말의 항고지혈증 효과에 관한 연구 : Triton WR-1339 주사에 의한 고지혈증 유발 생쥐의 간세포내 지방 축적 감소를 중심으로 Based on the Decreasing Effect of Lipid Accumulation in Hepatocyte of Murine with Hyperlipidemia induced by Triton WR-1339

박인식,안상현,정재만,강윤호,이해풍,서귀문,홍용기,김호현,김진택 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.7 No.2

본 연구는 긴볼레기말 추출물의 항고지혈증 효과를 조사하기 위해 ICR 생쥐에 TritonWR-1339(TX) 복강주사로 인위적인 고지혈증을 유발시킨 후 긴볼레기말 추출물(30㎎/㎏)를 복강주사하여 시간의 경과에 따른 간세포내에서의 지방 축적 변화를 조직화학적으로 관찰하였다. TX 주사후 그물구조의 세포질출 가진 간세포가 간엽 전체에서 관찰되었고, 일부 간소엽에서는 간세포 손상으로 인한 간세포판 소실이 나타났다. 또한 간세포내 지방축척도 증가하여 전체 간소엽의 간세포에서 지방의 과출현을 확인 할 수 있었고, 지방의 크기도 대조군에 비해 증가된 것으로 관찰되었다. 그러나 긴볼레기말 추출물 주사군에서는 그물구조의 세포질을 가진 간세포의 수가 TX 주사군에 비해 감소되었고, 대부분의 간소엽에서 정상적인 간세포판의 배열을 확인할 수 있었다. 간세포내의 지방 축적과 크기도 감소된 경향으로 관찰되었다. 이상의 결과로 볼 때 해조류 긴볼레기말 추출물은 고지혈증이 유발된 생쥐 간세포 내에서의 과도한 지방축적을 감소시키는 항고지혈증 효과를 하는 것으로 사료된다. Hepatic tissues of ICR mouse were intraperitoneally injeced with Colpomenia bullosa(CB) Extract after Triton WR-1339(TX) injection were observed to investigate the antihyperlipermic effect of CB extract for hyperlipidemic hepatic tissue caused by destruction of lipid metabolism. The hepatic tissues were obtained at hour-24, 48, and 72 after TX injection with CB extract treatment. And then these specimen were fixed in 10% neutral buffer solution and were cryocut. The tissue stained by H&E for general morphology and sudan black B for lipid distribution. The increase of hepatocyte having rneshlike cytoplasm were shown in all hepatic lobules after TX injection and the hepatic plates were disappeared in the region of meshlike hepatocyte aggregation, But the hepatocyte having meshlike cytoplasm were disappeared and hapatic plate were rearranged in CB extract injected mouse. The number of blue black colored lipid drop in hepatic cytoplasm of mouse injected with TX were increased and the size of lipid drop were enlarged. But the number of lipid drop in hepatic cytoplasm of mouse treated CB extract were decreased and the size of lipid drop were diminished. As results indicated that the accumulation of lipid drop caused by TX injection were mitigated by the antihyperlipidermic effect of CB extract.

連翹敗毒散加味方이 알러지성 접촉피비염에 미치는 影響 : DNCB 재노출부위의 생쥐 피부손상완화와 비만세포 분포 변화를 중심으로 Based on the mitigation of skin damage and distributive change of mast cell on DNCB re-expasure region of Mice

권오성,김진택,박인식,안상현,이해풍,김호현,강윤호 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.8 No.1

본 실험은 連翹敗毒散加味方이 알러지성 접촉피부염 유발시 발생되는 면역과민반응과 염증반응 저해에 미치는 영향을 조사하기위해 행해진 것으로 2,4-dinitrochlorobenzene(DNCB, 5% DNCB 25㎕로 감작, 7일경과 후 2.5% DNCB 5㎕로 유발) 처리로 알러지성 접촉피부염이 유발된 ICR계 암컷 생쥐에 連翹敗毒散加味方抽出物(0.51g/㎏/day)을 음용투여 한 다음 contact hypersensitivity assay, 피부의 일반적인 구조 그리고 비만세포의 분포 변화를 관찰하였다. Contact hypersensitivity assay의 결과, 連翹敗毒散加味方抽出物 투여군의 ear swelling이 DNCB군에 비해 유의성있게 감소한 것으로 나타났다. 알러지성 접촉피부염 유발 후 관찰되는 피부손상은 48시간에 제일 심한 것으로 관찰되었으나, 連翹敗毒散加味方抽出物 투여군에서는 DNCB군 비해 완화된 결과를 보였다. 連翹敗毒散加味方抽出物 투여군의 진피에서 림프구를 비롯한 염증관여세포의 이주와 혈관생성 그리고 표피내로의 림프구의 침윤 등이 감소되었고, 표피 기저층과 가시층세포에서는 세포질내 액포화와 세포사이 공간 확장도 줄어든 것으로 관찰되었다. 한편 알러지성 접촉피부염 유발 후 분포가 증가한 비만세포는 형태가 주로 세포내 함유물이 분비된 degranulated type로 나타났다. 이에 반해 連翹敗毒散加味方抽出物 투여군는 DNCB군에 비해 출현 비만세포'수가 일부 감소했고, 비만세포의 형태는 granulated t-ype였다. 또한 serotonin 면역조직화학적 염색결과로 連翹敗毒散加味方抽出物 투여군에서 se-rotonin 양성반응성이 높은 granulated type의 비만 세포가 주로 관찰되었다. 이상의 결과로 미루어 連翹敗毒散加味方抽出物은 알러지성 접촉피부염으로 유발된 피부 손상과 염증을 완화시키는 효과가 있는 것으로 사료된다. After allergic contact dermatitis elicitated by Dinitrochlorobenzene(DNCB) treatment, ICR female mice administered Yunkyopaedocksangamibang(YPGM) extract were observed to investigate the effect of YPGM on allergic contact dermatitis. This study investigated that contact hypersensitivity assay, abdominal skin morphologic changes including mast cells. At contact hypersensitivity assay, the right ear swelling in YPGM group were probability decrease than DNCB group. At obsevation of abdominal skin morphologic change, the infilteration of lymhocyte, lymphocyte insertion to epithelium, enlarged capillary, angiogenesis, and damages of epithelium as cytoplasmic vacuolation and enlarge of inter cellular space in YPGM were diminshed than DNCB group. The number of mast cell was increased both DNCB and YPGM group. The shape of mast cell in DNCB group was mainly appeared degranulated type, but granulated type in YPGM group. The number of serotonin positive cell was increased both DNCB and YPGM group. The shape of serotonin positive cell in DNCB group was mainly appeared degranuled type, but granulated type in YPGM group. As results indicated that the YPGM exrtact administration work on the mitigation of skin damages in mouse with allergic contact dermatitis.

Lipopolysaccharide로 유발된 생쥐 무릎 관절염 완화에 미치는 진통산의 효과 : 윤활관절막과 섬유관절막의 형태 변화를 중심으로 Based on the Morphological Change of Synovial Membrane and Fibrous Membrane

김진택,최홍식,안상현,박인식,강윤호,김호현,이해풍 동국대학교 경주대학 1999 東國論集 Vol.18 No.-

Knee joint BALB/C mice were administered with JINTONGSAN(JTS) extract after Lipopolysaccaride(LPS) injection were observed to investigate the anti-inflammatiory effect of JTS for arthritis. The JTS extract(3.3 ㎖/㎏/day) were daily administered to mice suffered from arthritis of knee joint induced by LPS injection, as dose of 300㎖/㎏. The specimen were fixed in 4% paraformaldehyde and were decalcificate in EDTA solution for 4 Weeks. In synovial membrane, the hyperplasia of synovial lining cell(fibroblast like synoviocytes) on JTS group were diminished then LPS group. The number of synovial lining cell(synovial phagocytic cells) with filopodia and the infiltrated of lymphocyte on JTS group were decreased. In fibrous membrane, The region of fibrosis on JTS group were mitigated than LPS group and the appearance of fibroblast and migrated cell, as neutrophil leukocytes and lymphocyte, were decreased. As results indicated that JTS was effective in anti-inflammatory reaction for LPS-induced arthritis.

혈부축어탕의 고지혈증 유발 생쥐 신장내 지방 축적 감소효과

김호현,강윤호,방혜정,김진택,박인식,안상현,이해풍 世明대학교 한의학연구소 1998 韓醫學硏究所 論文集 Vol.1 No.-

Renal tissues of ICR mouse were intraperitoneally injected with Hyulboochucketang(HCE;1.28g/kg/day) after Triton WR-1339(TX;600mg/kg) injection were observed to investigate the lipid accumulation suppressive effect of HCE. The renal tissues were obtained at hour 24,48 and 72 after TX injection that were fixed in fromol-calcium solution and were cryocut. The tissue stained by H&E for general morphology, sudan black B for lipid and perchloric acid-naphthoquinone method for cholesterol. The parietal layer of Bowman's capsule is swelled as cuboidal type at hour 48 after TX injection and the lipid blot and cholesterol particle were noticeably increased in glomerulus than control group. But the parietal layer of Bowman's capsule in HCE treated group was shaped as squamous type that was looked like as normal morphology and the lipid blot and cholesterol particle w3ere considerably decreased in glomerulus than TX group. As results indicated that the accumulation of lipid including cholesterol caused by TX injection were mitigated in renal tissues by the antihyperlipidermic effect of HCE.