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Jayant Nirmalkar,Zohaib Ul Hassan,Dongju Park,Seil Kim,Jinsang Jung 한국대기환경학회 2021 한국대기환경학회 학술대회논문집 Vol.2021 No.10
The diversity of the bacterial community and chemical composition in fresh snowfall samples has not yet been studied well, despite its importance for human health and climate change. We performed a comprehensive assessment of microbial community structures and chemical characteristics of fresh snowfall samples collected in Daejeon, Republic of Korea during winter 2020-2021. We utilized a cutting-edge genomic next-generation sequencing (NGS) technology to investigate the bacterial community in fresh snow sample. The microbial community classes of the fresh snowfall samples have consisted of three major phyla: Firmicutes (59%), Actinobacteria (30%), and Proteobacteria (8%). The concentration of water-soluble ions including (SO₄<SUP>2-</SUP> and NO₃-), dust tracers (Mg<SUP>2+</SUP> and Ca<SUP>2+</SUP>) and sea salt (Cland Na+), and water-soluble organic carbon were quantified significantly in fresh snowfall samples. The air masses passing over from the Gobi Desert incorporated halotolerant bacteria which are related to Firmicutes, Actinobacteria, and Proteobacteria strains that were potentially influenced by Asian dust, oceanic and urban emissions. Pseudomonas species, which is known as Ice nucleating bacteria in the atmosphere, was measured in fresh snowfall samples at this site.
Comparison of Digital PCR and Quantitative PCR with Various SARS-CoV-2 Primer-Probe Sets
( Changwoo Park ),( Jina Lee ),( Zohaib Ul Hassan ),( Keun Bon Ku ),( Seong-jun Kim ),( Hong Gi Kim ),( Edmond Changkyun Park ),( Gun-soo Park ),( Daeui Park ),( Seung-hwa Baek ),( Dongju Park ),( Jih 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.3
The World Health Organization (WHO) has declared the coronavirus disease 2019 (COVID-19) as an international health emergency. Current diagnostic tests are based on the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method, which is the gold standard test that involves the amplification of viral RNA. However, the RT-qPCR assay has limitations in terms of sensitivity and quantification. In this study, we tested both qPCR and droplet digital PCR (ddPCR) to detect low amounts of viral RNA. The cycle threshold (CT) of the viral RNA by RT-PCR significantly varied according to the sequences of the primer and probe sets with in vitro transcript (IVT) RNA or viral RNA as templates, whereas the copy number of the viral RNA by ddPCR was effectively quantified with IVT RNA, cultured viral RNA, and RNA from clinical samples. Furthermore, the clinical samples were assayed via both methods, and the sensitivity of the ddPCR was determined to be equal to or more than that of the RT-qPCR. However, the ddPCR assay is more suitable for determining the copy number of reference materials. These findings suggest that the qPCR assay with the ddPCR defined reference materials could be used as a highly sensitive and compatible diagnostic method for viral RNA detection.