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( Zhenghao Wang ),( Chunying Liu ),( Hongshan Yu ),( Bo Wu ),( Baoyu Huai ),( Ziyu Zhuang ),( Changkai Sun ),( Longquan Xu ),( Fengxie Jin ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.4
In this study, to obtain icaritin with high pharmacological activities from icariin, which has a content ratio of over 58% in the total flavonoids of Epimedium herb, a special Epimedium flavonoid-glycosidase was produced, purified and characterized from Aspergillus sp.y848 strain. The optimal enzyme production was gained in a medium containing 5% (w/v) wheat bran extract and 0.7% (w/v) Epimedium leaf powder as the enzyme inducer, and strain culture at 30℃ for 6-7 days. The molecular weight of the enzyme was approximately 73.2 kDa; the optimal pH and temperature were 5.0 and 40°C. The enzyme Km and Vmax values for icariin were 15.63 mM and 55.56 mM/h. Moreover, the enzyme hydrolyzed the 7-O-glucosides of icariin into icariside II, and finally hydrolyzed 3-Orhamnoside of icariside II into icaritin. The enzyme also hydrolyzed 7-O-glucosides of epimedin B to sagittatoside B, and then further hydrolyzed terminal 3-O-xyloside of sagittatoside B to icarisiede II, before finally hydrolyzing 3-O-rhamnoside of icarisiede II into icaritin. The enzyme only hydrolyzed 7-O-glucoside of epimedin A or epimedin C into sagittatoside A or sagittatoside C. It is possible to prepare icaritin from the high-content icariin in Epimedium herb using this enzyme. When 2.5% icariin was reacted at 40℃ for 18-20 h by the low-cost crude enzyme, 5.04 g icaritin with 98% purity was obtained from 10 g icariin. Also, the icaritin molar yield was 92.5%. Our results showed icaritin was successfully produced via cost-effective and relatively simple methods from icariin by crude enzyme. Our results should be very useful for the development of medicines from Epimedium herb.
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Changhao Wang,Yu Luo,Jinru Luo,Xiao Song,Wei Zhang,Pinghuai Wang,Zhenghao Liu,Maoyin Wang,Xinghui He,Linjiang Chai 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.4
Titanium alloys are one of the most important structural materials for the aerospace applications, which bring active studyon microstructural and mechanical optimization of the alloys. In the present study, commercial TA2 titanium sheets withdifferent initial textures were rolled to a strain of 4% at liquid nitrogen temperature. Microstructures of the rolled sheets werecharacterized using a scanning electron microscope equipped with an EBSD detector and their mechanical properties weretested through quasi-static uniaxial tension tests at room temperature. The influence of the initial texture on the microstructureand mechanical performance of the cryorolled sheet were discussed. Twinning is found to occur in the TA2 sheets after rollingeven at a small strain. Initial texture is an important factor affecting both numbers and types of the twins. {11–22} contractiontwinning is always the dominating type of twins for all three sheets having different initial textures. A lot of {11–22}and {11–24} twins can be found in the sheets with the initial texture of C-axes//ND, while the sheet with an initial texture ofC-axes away from the ND contains few twins, with a high density of dislocations developed instead. Along with contractiontwinning, many {10–12} and {11–21} twins are detected in the sheet with the initial texture of C-axes⊥ND. The cryorolledsheet having the densest twins shows the best mechanical properties, indicating a beneficial effect of a small pre-strain.
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UNDERSTANDING PEDESTRIANS’ CAR-HAILING INTENTION IN TRAFFIC SCENES
Linhui Li,Zhenghao Wang,Jing Lian,Yafu Zhou 한국자동차공학회 2022 International journal of automotive technology Vol.23 No.4
This study aims at the automatic understanding of pedestrians’ car-hailing intention in traffic scenes. Traffic scenes are highly complex, with a completely random spatial distribution of pedestrians. Different pedestrians use different behavior to express car-hailing intention, making it difficult to accurately understand the intention of pedestrians for autonomous taxis in complex scenes. A novel intention recognition algorithm with interpretability is proposed in this paper to solve the above problems. Firstly, we employ OpenPose to obtain skeleton data and the facial region. Then, we input the facial region into a facial attention network to extract the facial attention features and infer whether the pedestrian is paying attention to the ego-vehicle. In addition, the skeleton data are also input into a random forest classifier and GCN to extract both explicit and implicit pose features. Finally, an interpretable fusion rule is proposed to fuse the facial and pose features. The fusion algorithm can accurately and stably infer the pedestrians’ intention and identify pedestrians with car-hailing intentions. In order to evaluate the performance of the proposed method, we collected road videos using experimental cars to obtain suitable datasets, and established the corresponding evaluation benchmarks. The experimental results demonstrate that the proposed algorithm has high accuracy and robustness.
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Zheng, Hao,Zhang, Qing,Kim, Seung-Joo,Jiang, Xueya,Dan, Meiyu,Gao, Hong,Li, Shengbiao,Wang, Shiquan,Feng, Chuanqi American Scientific Publishers 2013 Journal of Nanoscience and Nanotechnology Vol.13 No.4
<P>KMn8O16 nanorods were prepared through a facile hydrothermal method by using KMnO4 and MnSO4 as reactants. The KMn8O16 samples synthesized at different temperatures (100-160 degrees C) were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), and its electrochemical properties were tested by galvanostatic charge/discharge system. The effect of reaction temperature on the morphology and electrochemical properties was investigated. As electrode materials for the lithium ion battery cycled between 1.5 and 4.2 V, the KMn8O16 nanorods synthesized at 160 degrees C show the highest reversible discharge capacity (160.1 mA h/g even after 50 cycles at current density of 50 mA/g) and the best cycling stability. These results indicate that the KMn8O16 nanorods could be a promising cathode material for lithium ion batteries.</P>
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