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Adenoviral Vector Mediates High Expression Levels of Human Lactoferrin in the Milk of Rabbits
( Zeng Sheng Han ),( Qing Wang Li ),( Zhi Ying Zhang ),( Yong Sheng Yu ),( Bo Xiao ),( Shu Yun Wu ),( Zhong Liang Jiang ),( Hong Wei Zhao ),( Rui Zhao ),( Jian Li ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.1
The limitations in current technology for generating transgenic animals, such as the time and the expense, hampered its extensive use in recombinant protein production for therapeutic purpose. In this report, we present a simple and less expensive alternative by directly infusing a recombinant adenovirus vector carrying human lactoferrin cDNA into rabbit mammary glands. The milk serum was collected from the infected mammary gland 48 h post-infection and subjected to a 10% SDS-PAGE and Western blotting. An 80-kDa protein was visualized after viral vector infection. With this method, we obtained a high level of expressed human lactoferrin of up to 2.3 mg/ml in the milk. Taken together, the method is useful for the transient high-level expression recombinant proteins, and the approach established here is probably one of the most economical and efficient ways for large-scale production of recombinant proteins of biopharmaceutical interest.
Yu-Ping Hua,Hong-Liang Chai,Si-Yuan Yang,Xiang-Wei Zeng,Ying Sun 대한수의학회 2005 Journal of Veterinary Science Vol.6 No.4
Two hundred thirty specimens of wild birds were collected from some areas in Heilongjiang Province during the period of 2003~2004, including two batches of specimens collected randomly from a same flock of mallards in Zhalong Natural Reserve in August and December, 2004, respectively. Primary virus isolation and identification for avian influenza virus (AIV) and Newcastle disease virus (NDV) were performed. The results showed that only two specimens of young mallards collected from Zhalong Natural Reserve in August, 2004 were positive to AIV (isolation rate 0.9%), and one strain (D57) of these two virus isolates was identified to be H9 subtype by hemagglutination inhibition test. Meanwhile, the two batches of blood serum samples of mallards from Zhalong were also examined for antibodies against AIV and NDV. Among 38 blood serum samples collected in August, antibodies against the hemagglutinin of H1, H3, H5, H6 and H9 subtypes of AIV were found in 1, 0, 2, 0 and 8 samples, respectively; and 11 samples were found with antibody against NDV. Whereas the NDV isolation in both two batches of specimens of mallard was negative, all of the 32 blood serum samples collected in December were negative for antibodies against AIV and NDV.
Zeng-Hui Yue,Xin-Qun He,Xiao-Rong Chang,Jian-Ling Yuan,Bao-Sheng Yu,Mi Liu,Ling Fu,Liang Zhang,Li-Chao Shang 사단법인약침학회 2012 Journal of Acupuncture & Meridian Studies Vol.5 No.2
Objective: To explore the mechanism of Toll-like receptor (TLR4) inhibition in the delay of formation of atherosclerosis by herb-partition moxibustion. Method: Seventy-five rabbits were randomly assigned to one of five groups: blank, atherosclerosis (AS) model, direct moxibustion, herb-partition moxibustion, and drug treatment. With the exception of the blank group, all rabbits were given a high-fat diet in addition to immunologic injury to create the AS model. The experiments were carried out for 16 weeks, at which time the aorta was removed from each rabbit. Immunohistochemical methods were used to detect the gray level of the aortic TLR4 to observe the immunologic competence of its antigens. Fluorescent quantitative polymerase chain reaction was used to detect the expression of TLR4 messenger RNA (mRNA) in the aorta. Results: The gray-scale value of TLR4 and the TLR4 mRNA expression significantly decreased (p < 0.05) in the direct moxibustion, herb-partition moxibustion, and drug treatment groups. Furthermore, the effects of the herb-partition moxibustion and drug treatment were superior to those of the direct moxibustion. Conclusion: Herb-partition moxibustion inhibits aortic TLR4 activity and mRNA expression,showing that herb-partition moxibustion delays the formation of atherosclerosis through the inhibition of TLR4 expression.
A Novel 3-D Imaging Configuration Exploiting Synthetic Aperture Ladar
Liang Guo,Yinli Huang,Xiaozhen Li,Xiaodong Zeng,Yu Tang,Mengdao Xing 한국광학회 2017 Current Optics and Photonics Vol.1 No.6
Traditional three-dimensional (3-D) laser imaging systems are based on real aperture imaging technology,whose resolution decreases as the range increases. In this paper, we develop a novel 3-D imaging techniquebased on the synthetic aperture technology in which the imaging resolution is significantly improved anddoes not degrade with the increase of the range. We consider an imaging laser radar (ladar) system usingthe floodlight transmitting mode and multi-beam receiving mode. High 3-D imaging resolutions areachieved by matched filtering the linear frequency modulated (LFM) signals respectively in range, syntheticaperture along-track, and the real aperture across-track. In this paper, a novel 3-D imaging signal modelis given first. Because of the motion during the transmission of a sweep, the Doppler shift induced bythe continuous motion is taken into account. And then, a proper algorithm for the 3-D imaging geometryis given. Finally, simulation results validate the effectiveness of the proposed technique.
Wei Liang,Zeqiang Zhang,Tao Yin,Yanqing Zeng,Yu Zhang 한국정밀공학회 2024 International Journal of Precision Engineering and Vol.11 No.1
In most resource recycling enterprises, waste electrical and electronic equipment (WEEE) recycling is performed in a multi-parallel disassembly line layout with mixed-product disassembly mode. Thus, this study constructs a mixed-integer non-linear programming (MINLP) and implements a multi-objective improved ant lion optimizer (MIALO) to minimize four objectives, namely, the number of shared workstations, workstation load balancing, demand, and hazard indices, of the mixed-product multi-parallel disassembly line balancing problem (MPDLBP). For MIALO, the ant population initialization and population interaction operations are improved to enhance the global optimization capability of MIALO. In addition, the Pareto and non-dominated sorting genetic algorithm (NSGA-II) are integrated to maintain the number of population. Furthermore, the MINLP is solved by GUROBI 9.1.1, and the results are compared with those of MIALO to verify the correctness of MIALO. To further demonstrate the performance of MIALO, the MIALO is compared with four existing algorithms. Finally, the MIALO is applied to optimize a multi-parallel disassembly case of WEEE. Then, several productive disassembly schemes are provided to recycle waste TV sets.
Shi, Liang,Wu, Li-Li,Yang, Jian-Rong,Chen, Xiao-Fei,Zhang, Yi,Chen, Zeng-Qiang,Liu, Cun-Li,Chi, Sheng-Ying,Zheng, Jia-Ying,Huang, Hai-Xia,Yu, Fu-Jun,Lin, Xiang-Yang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7
Background: Recently, peroxiredoxin3 (PRDX3) was identified as a novel molecular marker for the progression of hepatocellular carcinoma (HCC). However, its potential clinical application as a serum marker for the early diagnosis and prognosis of HCC has not been investigated. Methods: PRDX3, alpha-fetaprotein (AFP), and other biochemical parameters were measured in serum samples from 297 Chinese patients, including 96 with HCC, 98 with liver cirrhosis (LC), and 103 healthy controls (HCs). Correlations between serum PRDX3 expression and clinicopathological variables and the relationship between serum PRDX3 expression and prognosis were analyzed. Results: Serum PRDX3 was significantly higher in HCC patients than in the LC and HC groups. The sensitivity and specificity of serum PRDX3 for the diagnosis of HCC were 85.9% and 75.3%, respectively, at a cutoff of 153.26 ng/mL, and the area under the curve was 0.865. Moreover, serum PRDX3 expression was strongly associated with AFP level, tumor diameter, TNM stage, and portal vein invasion. Kaplan-Meier curve analysis revealed that HCC patients with high serum PRDX3 expression had a shorter median survival time than those with low PRDX3 expression. Moreover, serum PRDX3 expression was an independent risk factor for overall survival. The inverse correlation between serum PRDX3 and patient survival remained significant in patients with early-stage HCC and in those with normal serum AFP levels. Conclusions: Serum PRDX3 can be used as a noninvasive biomarker for the diagnosis and/or prognosis of HCC.
Effects of β Air Cooling and Subsequent Cold Rolling on Microstructure and Hardness of Zr702 Sheet
Haotian Guan,Lingguo Zeng,Zhijun Li,Linjiang Chai,Yufan Zhu,Yueyuan Wang,Qin Huang,Ke Chen,Liang‑yu Chen,Ning Guo 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.2
In this work, a Zr702 sheet was subjected to β air cooling and then rolled to 15% reduction at room temperature, with theirdetailed microstructural characteristics characterized by electron channeling contrast imaging and electron backscatterdiffraction techniques. Results show that after the β air cooling, the prior equiaxed grains in the as-received material arecompletely transformed into Widmanstätten structures featured by coarse plates with typical phase transformation misorientationsbetween them. The subsequent 15% rolling allows both slip and twinning (especially the {10–12} type) to beactivated readily, leading to significant grain refinement and the appearance of misorientation angle peaks around 3°–5° and85°. Analyses on kernel average misorientations reveal that there exist very low residual strains in the β-air-cooled specimenwhile they are markedly increased after the 15% rolling and preferably distributed near low-angle and twin boundaries. Hardnessmeasurements show that the specimen hardness is evidently decreased from ~ 199 to ~ 170 HV after the β air cooling,which can be attributed to grain coarsening and the scattered orientations associated with the slow β → α transformation. For the 15%-rolled specimen, however, effective grain refinement by twinning and denser low-angle boundaries jointly leadto ~ 35% hardness increment to ~ 228 HV.
Protective effect of adiponectin on paraquat-induced pulmonary fibrosis in mice
Rong Yao,Yarong He,Zhi Zeng,Zongan Liang,Yu Cao 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.2
Pulmonary fibrosis (PF) is the most common complication of paraquat (PQ) toxicity, which lacks an effective treatment. This study aimed to investigate whether exogenous administration of globular adiponectin (APN) isoform provided protection against PQ-induced PF, and examined the possible mechanisms underlying these effects. Eighty BALB/C mice were randomly divided into control, PQ, lowdose APN, and high-dose APN groups. Human lung WI-38 fibroblasts were similarly divided into control, PQ, and APN groups and examined at 24 h, 48 h, and 72 h after PQ exposure. Hematoxylin and eosin (HE) and Masson trichrome staining were used to compare the histopathologic changes in the mouse lung tissues. Western blot and real-time quantitative-PCR (RTPCR) were used to measure the protein and mRNA expression of transforming growth factor β1 (TGF-β1) in mice lung tissues and α-SMA, type III collagen, and NF-κB p65 in lung fibroblasts. Dihydroethidium (DHE) was used to detect intracellular superoxide anion (O2-) in fibroblasts. APN administration significantly ameliorated PQ-mediated fibrosis histologically and reduced the protein and mRNA expression levels of TGF-β1 in mouse lung tissues in a dose-dependent manner (P<0.05). When fibroblasts were pretreated with APN, the expression of α-SMA and NF-κB p65 were down-regulated, and O2- decreased. Expression of the anti-inflammatory factor IL-1Ra was upregulated following PQ exposure (P<0.05). This study revealed the beneficial effects of APN against PQ-induced PF, which may have occurred through suppression of NF- κB dependent inflammatory and TGF-β1 mediated fibrotic events