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김대중,Yuzuru Suzuki,Katsumi Aida 한국수산과학회 2011 Fisheries and Aquatic Sciences Vol.14 No.4
The mechanism by which gonadotropin-releasing hormone (GnRH) and dopamine (DA) control gonadotropin (GTH) release was studied in male and female rainbow trout using cultured pituitary cells obtained at different reproductive stages. The mechanisms of follicle-stimulating hormone (FSH) release by GnRH and DA could not be determined yet. However, basal and salmon-type GnRH (sGnRH)- or chicken-II-type GnRH (cGnRH-II)- induced luteinizing hormone (LH) release increased with gonadal maturation in both sexes. LH release activity was higher after sGnRH stimulation than cGnRH-II stimulation at maturing stages in both sexes. The GnRH antagonist ([Ac-3, 4-dehydro-Pro^1, D-p-F-Phe^2, D-Trp^(3,6)] GnRH) suppressed LH release by sGnRH stimulation in a dose-dependent manner, although the effect was weak in maturing fish. The role of DA as a GTH-release inhibitory factor differs during the reproductive cycle: the inhibition of sGnRH-stimulated LH release by DA was stronger in immature fish than in maturing, ovulating, or spermiated fish. DA did not completely inhibit sGnRH-stimulated LH release, and DA alone did not alter basal LH release. Relatively high doses (10^(-6) or 10^(-5)M) of domperidone (DOM, a DA D2 antagonist) increased LH release, which did not change with reproductive stage in either sex. The potency of DOM to enhance sGnRH-stimulated LH release was higher in maturing and ovulated fish than in immature fish. These data suggest that LH release from the pituitary gland is controlled by dual neuroendocrine mechanisms by GnRH and DA in rainbow trout, as has been reported in other teleosts. The mechanism of control of FSH release, however, remains unknown.
Kim, Dae-Jung,Suzuki, Yuzuru,Aida, Katsumi The Korean Society of Fisheries and Aquatic Scienc 2011 Fisheries and Aquatic Sciences Vol.14 No.4
The mechanism by which gonadotropin-releasing hormone (GnRH) and dopamine (DA) control gonadotropin (GTH) release was studied in male and female rainbow trout using cultured pituitary cells obtained at different reproductive stages. The mechanisms of follicle-stimulating hormone (FSH) release by GnRH and DA could not be determined yet. However, basal and salmon-type GnRH (sGnRH)- or chicken-II-type GnRH (cGnRH-II)- induced luteinizing hormone (LH) release increased with gonadal maturation in both sexes. LH release activity was higher after sGnRH stimulation than cGnRH-II stimulation at maturing stages in both sexes. The GnRH antagonist ([Ac-3, 4-dehydro-$Pro^1$, D-p-F-$Phe^2$, D-$Trp^{3,6}$] GnRH) suppressed LH release by sGnRH stimulation in a dose-dependent manner, although the effect was weak in maturing fish. The role of DA as a GTH-release inhibitory factor differs during the reproductive cycle: the inhibition of sGnRH-stimulated LH release by DA was stronger in immature fish than in maturing, ovulating, or spermiated fish. DA did not completely inhibit sGnRH-stimulated LH release, and DA alone did not alter basal LH release. Relatively high doses ($10^{-6}$ or $10^{-5}M$) of domperidone (DOM, a DA D2 antagonist) increased LH release, which did not change with reproductive stage in either sex. The potency of DOM to enhance sGnRH-stimulated LH release was higher in maturing and ovulated fish than in immature fish. These data suggest that LH release from the pituitary gland is controlled by dual neuroendocrine mechanisms by GnRH and DA in rainbow trout, as has been reported in other teleosts. The mechanism of control of FSH release, however, remains unknown.
OKUMURA, TAKUJI,HAN, CHANG-HEE,SUZUKI, YUZURU,AIDA, KATSUMI,HANYU, ISAO 동의대학교 기초과학연구소 1993 基礎科學硏究論文集 Vol.3 No.1
Hemolymph vitellogenin and ecdysteroid levels were traced during the reproductive and non-reproductive molt cycles in adult females of the freshwater prawn Macrobrachium nipponense, in which the reproductive development coincides with the molt cycle. During the reproductive molt cycle, vitellogenin levels began to increase at stage B. High levels were maintained during stages C₁-D₃ in concomitance with ovarian growth, and these levels decreased following the prespawning ecdysis. Vitellogenin was at undetectable levels during the non-reproductive molt cycle, and correspondingly, ovarian maturation did not advance. The predominant ecdysteroids in the hemolymph of M. nipponense were determined by HPLC-RIA analysis as 20-hydroxyecdysone (20E) and immunoreactive high polarity products(HPP). 20E and HPP levels peaked at stage D₃ during both molt cycles. 20E levels declined rapidly to low levels after ecdysis in the reproductive molt cycle. On the other hand, 20E levels decreased slowly during stages A-C₁in the non-reproductive molt cycle. HPP levels immediately declined prior to spawning at stage A_0 and showed a small peak after spawning at stage A₁in the reproductive molt cycle, whereas in the non-reproductive molt cycle levels were still high at stage A and declined slowly during stages B-C₁. These differences in ecdysteroid levels suggest a possible relationship between ecdysteriod levels and vitellogenesis.
Han, Chang-Hee,Okumura, Takuji,Suzuki, Yuzuru,Aida, Katsumi,Hanyu, Isao 동의대학교 기초과학연구소 1995 基礎科學硏究論文集 Vol.5 No.1
The site of vitellogenin synthesis in the freshwater prawn Macrobrachium nipponense was examined immunocytochemically using antibodies raised against lipovitellin purified from mature M. nipponense ovaries. The molecular weight of purified lipovitellin was estimated to be 350kDa by gel filtration. The antiserum cross-reacted with vitellogenin in the hemolymph of the mature female prawn, and could therefore be used to demonstrate the possible site of vitellogenin synthesis immunocytochemically employing the peroxidase-anti-peroxidase(PAP) reaction. Hepatopancreas, sub-epidermal tissue, muscle, and ovary were fixed with periodate-lysine paraformaldehyde solution after which parapffin sections were made. In mature females, oocytes at the yolk globule stage, the hepatopancreas and the sub-epidermal tissue of the lateral somites showed positive PAP reaction. However, the sub-epidermal tissue of the ventral somites, follicular cells of the ovary and muscles did not. No positive reaction was observed in males and immature females. These results suggest that vitellogenin is synthesized in the hepatopancreas and at the sub-epidermal tissue in females at the exogenous vitellogenesis stage.