Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

Elevated TRAF2/6 expression in Parkinson's disease is caused by the loss of Parkin E3 ligase activity.

Chung, Ji-Yun,Park, Hee Ra,Lee, Su-Jin,Lee, Sun-Hye,Kim, Jin Sik,Jung, Youn-Sang,Hwang, Sang Hyun,Ha, Nam-Chul,Seol, Won-Gi,Lee, Jaewon,Park, Bum-Joon United States and Canadian Academy of Pathology [e 2013 Laboratory investigation Vol.93 No.6

<P>Parkinson's disease (PD) is the second leading neurodegenerative disease, and is known to be induced by environmental factors or genetic mutations. Among the verified genetic mutations of PD, Parkin, isolated from the PARK2 locus, shows an autosomal recessive inheritance pattern and is known to be an E3 ligase. However, the physiological target of Parkin and the molecular mechanism of Parkin-deficiency-induced PD have not been clearly demonstrated until now. It has recently been proposed that inflammation, suggesting as a causal factor for PD, is enhanced by Parkin deficiency. Thus, we examined the relationship between inflammation-related factors and Parkin. Here, we provide the evidence that Parkin suppresses inflammation and cytokine-induced cell death by promoting the proteasomal degradation of TRAF2/6 (TNF-α receptor-associated factor 2/6). Overexpression of Parkin can reduce the half-lives of TRAF2 and TRAF6, whereas si-Parkin can extend them. However, mutant Parkins did not alter the expression of TRAF2/6. Thus, loss of Parkin enhances sensitivity to TNF-α- or IL-1β-induced JNK activation and NF-κB activation. Indeed, si-Parkin-induced apoptosis is suppressed by the knockdown of TRAF6 or TRAF2. We also observed elevated expression levels of TRAF6 and a reduction of IκB in an 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced PD mouse model. Moreover, elevated expression levels or aggregation of TRAF6 were detected in approximately half of the human PD tissues (7/15 cases) and 2 cases, respectively. In addition, TRAF6 and Parkin expression levels show a reverse relationship in human PD tissues. Our results strongly suggest that the reduction of Parkin or overexpression of TRAF2/6 by chronic inflammation would be the reason for occurrence of PD.</P>

김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

정신분열증에서 신경증적 증상의 임상적 의의에 관한 연구

박병관,신석철,강민희,신윤오 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.1

To investigate the clinical significance of the neurotic symptoms in schizophrenia, the author applied PSE(Present State Examination) on Screening Schedule to 153 schizophrenic patients who were fit for the aim of this study. The patients were divided into two groups to grasp the phenomenological characteristics as the group with neurotic symptoms (100 cases) and the group without neurotic symptoms(53 cases). Among 91 items except neurotic symptoms in PSE, 14 items were selected as significant under X²-test, and tried factor analysis with 14 items using SPSS/PC+. The results of this study were summarized as follows. 1. In the group with neurotic symptoms, 4 factors were extracted as slowness of speech and behavior and blunted affect factor, inappropriate answer and affect factor, delusion factor, and restricted and poverty of speech factor. In the group without neurotic symptoms, 5 factors were extracted as slowness speech and behavior and incoherence factor, inappropriate and poverty speech and self-neglect factor, delusion factor, inappropriate affect factor, and loss of affect factor. In the group of total patients, 4 factor were extracted as restricted and slowness of speech and behavior and blunted affect factor, delusion factor, inappropriate and poverty of speech and inappropriate affect factor, and incoherence factor. 2. In the group with neurotic symptoms, slowness of speech and behavior, inappropriate answer, blunted and inappropriate affect were the major symptoms and inappropriate answer and affect, delusion, restricted and poverty of speech were the minor symptoms. Among these symptoms, slowness restriction, poverty of speech and behavior, inappropriate answer and characteristic blunted affect were characterized by negative symptoms(i.e. defect state) which were frequent in chronic schizophrenia. 3. In the group without neurotic symptoms, slowness of speech and behavior, incoherence, and self-neglect were the major symptoms and inappropriate and poverty of speech, delusion, inappropriate and loss of affect were the minor symptoms. Among these symptoms, slowness and inappropriateness of speech and behavior, and characteristic incoherence and self-neglect were characterized by positive symptoms which were interpreted as less distorted reality than the group with neurotic symptoms in the process of schzophrenia. 4. In the group of total patients, restricted and slowness of speech and behavior, blunted affect and delusion were the major symptoms and inappropriateness, poverty and incoherence of speech, and inappropriate affect were the minor symptoms, which were composed of the major and the minor symptoms of the group with neurotic symptoms and without neurotic symptoms. These symptoms can be seen frequently in the diagnostic criterias and clinical practice and thought to be the result of the major symptoms common in schizophrenia regardless of he presence of neurotic symptoms.

원형빌렛으로부터 인볼루트 헬리컬 핀을 가진 제품의 비틀림 압출가공공정에 관한 연구

박대윤,진철호,진인태 釜慶大學校 1999 釜慶大學校 論文集 Vol.4 No.1

The twisting and extrusion process of the product with involute helical fin from the round billet is developed by the upper bound analysis. The twisting of extruded product is caused by the twisted die surface connecting the die entrance section and the die exit section linearly. In the analysis, the internal shear surface is defined as the curved twisted plane from the taisting of die surface and the shear work is calculated by the consumption of shear energy The increase rate of angular velocity is determined by the minimization of plastic work. The results of the analysis. It is shown that the angular velocity of the extruded product increases with the die twisting angle, the reduction of area, and decreases with the die length, the friction condition.

원자력발전소 PSI/ISI 데이터 관리를 위한 지능형 데이터 베이스 프로그램 개발

박은수,박익근,엄병국,박윤원,강석철 韓國非破壞檢査學會 1998 한국비파괴검사학회지 Vol.18 No.5

Windows 95 환경하에서 PSI/ISI 데이터의 효율적 관리를 위한 종합지원시스템의 구축과 다원적평가와 분석이 가능하고, 비파괴검사에 의한 원전기기의 건전성 평가시 규제측면에서 기술적인 지원을 할 수 있는 고리 원전 PSI/ISI 데이터 베이스 구축과 프로그램을 개발하였다. 이 프로그램을 활용하면 현재까지 수행된 고리 원전 PSI/ISI의 수행현황과 지금까지 발견된 결함에 대한 조사분석이 가능하며, 향후 국내 PSI/ISI결과의 자료분석이 가능한 전문가 시스템(expert system)개발에 한단계 접근하게 되었다. 그리고, PSI/ISI에서 발견된 결함에 대한 조사분석 결과를 근거로 결함의 종류, 형태, 처리방법 등에 대한 자세한 분석을 통하여 국내 원전 PSI/ISI 결과의 확률론적 신뢰도 평가, 또는 확률론적 안전성평가를 위한 결함검출확률곡선의 작성과 위험도에 근거한 가동중검사제도의 도입에 필요한 기초 자료를 제공하였다. 더 나아가 지금까지의 자료분석을 바탕으로 보다 경제적이며 신뢰성이 높은 PSI/ISI의 수행으로 원전 압력경계기기의 건전성과 신뢰성 확보 및 수명예측을 위한 기초자료를 제공하였다. For an effective and efficient management of large amounts of preservice/inservice inspection(PSI/ISI) data in nuclear power plants, an intellegent Windows 95-based data management program was developed. This program enables the prompt extraction of previously conducted PSI/ISI conditions and results so that the time-consuming data management, painstaking data processing and analysis in the past are avoided. The program extracts, and the associated remedies. Furthermore, additional inspection data and comments can be easily added or deleted for subsequent PSI/ISI operation. Although the initial version of the program was applied to Kori nuclear power plant, this program can be equally applied to other nuclear power plant. And also this program can be used to offer the fundamental data for application of evaluation data related to fracture mechanics analysis(FMA), probabilistic reliability assessment(PRA) of PSI/ISI results, performance demonstration initiative (PDI) and risk-informed ISI based on probability of detection(POD) information of ultrasonic examination. Besides, the program can be further developed as a unique PSI/ISI data management expert system that can be apart of PSI/ISI data management expert system that can be a part of PSI/ISI Total Support System(TSS) for Korean nuclear power plants.

일부 운수사업장 근로자의 건강진단 유소견자의 사후관리 실행과 관련요인

윤혜은,박종,이영선,강성득,김철호 조선대학교 2003 The Medical Journal of Chosun University Vol.28 No.1

Objective : The purpose of this study was to find the relationship between the follow-up management status for medical problem to be treated and various characteristics of some workers at transportation business. Methods : A questionnaire survey was made toward 183 workers with medical problem(C and D2) among 945 examine in 1997. Results: Sixty four point five percent of surveyee received the examination result report and 40.5% were explained about the result 80.3% of surveyee answered that they knew the result of health examination. Most workers felt the necessity of the health examination, but 54.1% were dissatisfied with present health examination system and 66.7% pointed it out as nominal The rate of follow-up management for the medical problem was 62.8% By the multiple logistic regression the follow-up management rate for those who knew the health examination result were 2.98 times higher than those who didn't know the result (OR=2.98, 95% CI 1.41~6.29) The rate for those who perceived the medical problem already were 2.64 times higher than those who perceived the problem for the first time (OR=2.64, 95% CI 1.13~6.15). The rate for those who had subjective symptoms were 2.83 times higher than those who didn't have subjective symptoms. Conclusion : sixty two point eight percent of workers with medical problem performed the follow-up management and perception of health examination result, perception of the problem in the past and experience of subjective symptoms were related with the follow-up management.

마이크로스트립 지연선 여파기에 관한 연구

윤준식,박규호,윤철상,박준석,안달 호서대학교 반도체제조장비국산화연구센터 2000 학술대회 자료집 Vol.2000 No.1

일반적으로 LPA에서는 위상을 조정하기 위해 매우 긴 지연선을 필요로 한다. 지연성능은 고전력이나 저전력 모두에서 임의의 길이의 동축선로를 여러번의 과정을 거쳐 적용해봄으로써 구현된다. 그러나 동축선로형 지연선은 부피나 높은 삽입손실등의 제약이 많이 따른다. 본 논문에서는 마이크로스트립 형태의 지연선 대역통과 여파기를 설계하였고, 실험결과는 지연선여파기가 동축선로를 대신하여 LPA 시스템에 적용이 가능함을 보였다.

이차성 기질화 폐렴이 동반된 피부근염 1예

박철연,권정석,정진욱,이충기,현대성,최정윤 영남대학교 의과대학 2008 Yeungnam University Journal of Medicine Vol.25 No.2

Dermatomyositis is characterized by progressive, symmetric, Proximal muscle weakness and a nonsuppurative inflammatory myopathy of unknown etiology involving predominantly skeletal muscles. It is also characterized by typical skin lesions. Interstitial lung disease has a poor prognosis when it is associated with dermatomyositis. Organizing pneumonia is a disease in which granulation tissue fills the lumina of terminal and respiratory bronchioles and extends into the distal airspaces. The cryptogenic nature of the process is appreciated in that organizing pneumonia patterns of injury can be seen in secondary forms of the disease (secondary organizing pneumonia). Organizin pneumonia has been reported to occur in 5~10% in dermatomyositis-polymyositis patients Anti-histidyl tRNA synthetase antibody (anti-Jo-1) is a Predictive disease marker that is reported to occur in up to 70% of patients. We describe a 49-year-old male dermatomyosits patient who presented with organizing pneumonia and was found to have negative anti-Jo-1 antibody.

열 변화에 의한 구리전선의 미세조직 및 상 변화에 관한 연구(Ⅲ)

박성우,김윤회,김상현,박남규,김수철,김영만 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-