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Kim, Eun Hye,Kim, Youn Shic,Park, Su-Hyun,Koo, Yeon Jong,Choi, Yang Do,Chung, Yong-Yoon,Lee, In-Jung,Kim, Ju-Kon American Society of Plant Physiologists 2009 Plant Physiology Vol.149 No.4
<P>Jasmonic acid (JA) is involved in plant development and the defense response. Transgenic overexpression of the Arabidopsis (Arabidopsis thaliana) jasmonic acid carboxyl methyltransferase gene (AtJMT) linked to the Ubi1 promoter increased levels of methyl jasmonate (MeJA) by 6-fold in young panicles. Grain yield was greatly reduced in Ubi1:AtJMT plants due to a lower numbers of spikelets and lower filling rates than were observed for nontransgenic (NT) controls. Ubi1:AtJMT plants had altered numbers of spikelet organs, including the lemma/palea, lodicule, anther, and pistil. The loss of grain yield and alteration in spikelet organ numbers were reproduced by treating NT plants with exogenous MeJA, indicating that increased levels of MeJA in Ubi1:AtJMT panicles inhibited spikelet development. Interestingly, MeJA levels were increased by 19-fold in young NT panicles upon exposure to drought conditions, resulting in a loss of grain yield that was similar to that observed in Ubi1:AtJMT plants. Levels of abscisic acid (ABA) were increased by 1.9- and 1.4-fold in Ubi1:AtJMT and drought-treated NT panicles, respectively. The ABA increase in Ubi1:AtJMT panicles grown in nondrought conditions suggests that MeJA, rather than drought stress, induces ABA biosynthesis under drought conditions. Using microarray and quantitative polymerase chain reaction analyses, we identified seven genes that were regulated in both Ubi1:AtJMT and drought-treated NT panicles. Two genes, OsJMT1 and OsSDR (for short-chain alcohol dehydrogenase), are involved in MeJA and ABA biosynthesis, respectively, in rice (Oryza sativa). Overall, our results suggest that plants produce MeJA during drought stress, which in turn stimulates the production of ABA, together leading to a loss of grain yield.</P>
Oh, Se-Jun,Song, Sang Ik,Kim, Youn Shic,Jang, Hyun-Jun,Kim, Soo Young,Kim, Minjeong,Kim, Yeon-Ki,Nahm, Baek Hie,Kim, Ju-Kon American Society of Plant Physiologists 2005 Plant Physiology Vol.138 No.1
<P>Rice (Oryza sativa), a monocotyledonous plant that does not cold acclimate, has evolved differently from Arabidopsis (Arabidopsis thaliana), which cold acclimates. To understand the stress response of rice in comparison with that of Arabidopsis, we developed transgenic rice plants that constitutively expressed CBF3/DREB1A (CBF3) and ABF3, Arabidopsis genes that function in abscisic acid-independent and abscisic acid-dependent stress-response pathways, respectively. CBF3 in transgenic rice elevated tolerance to drought and high salinity, and produced relatively low levels of tolerance to low-temperature exposure. These data were in direct contrast to CBF3 in Arabidopsis, which is known to function primarily to enhance freezing tolerance. ABF3 in transgenic rice increased tolerance to drought stress alone. By using the 60 K Rice Whole Genome Microarray and RNA gel-blot analyses, we identified 12 and 7 target genes that were activated in transgenic rice plants by CBF3 and ABF3, respectively, which appear to render the corresponding plants acclimated for stress conditions. The target genes together with 13 and 27 additional genes are induced further upon exposure to drought stress, consequently making the transgenic plants more tolerant to stress conditions. Interestingly, our transgenic plants exhibited neither growth inhibition nor visible phenotypic alterations despite constitutive expression of the CBF3 or ABF3, unlike the results previously obtained from Arabidopsis where transgenic plants were stunted.</P>
Subcellular localization of rice histone deacetylases in organelles
Chung, Pil Joong,Kim, Youn Shic,Park, Su-Hyun,Nahm, Baek Hie,Kim, Ju-Kon Elsevier 2009 FEBS letters Vol.583 No.13
<P><B>Abstract</B></P><P>Histone deacetylases (HDACs) are known to function in the nucleus. Here, we report on the organellar localization of three rice HDACs, OsSIR2b, OsHDAC6, and OsHDAC10. The <I>35S:OsSIR2b-GFP</I> and <I>35S:OsHDAC10-GFP</I> constructs were introduced into tobacco BY2 cells. Co-localization analysis of the green fluorescent protein and MitoTracker fluorescent signals in the transformed BY2 cells indicated that OsSIR2b and OsHDAC10 are localized in the mitochondria. Transgenic Arabidopsis lines harboring <I>35S:OsHDAC6-GFP</I> and <I>35S:OsHDAC10-GFP</I> constructs were similarly analyzed, revealing that OsHDAC6-GFP is localized exclusively in chloroplasts, whereas OsHDAC10-GFP is localized in both mitochondria and chloroplasts. The presence of OsHDAC6-GFP and OsHDAC10-GFP in chloroplasts was verified by immunodetection.</P>
Analysis of five novel putative constitutive gene promoters in transgenic rice plants
Park, Su-Hyun,Yi, Nari,Kim, Youn Shic,Jeong, Min-Ho,Bang, Seung-Woon,Choi, Yang Do,Kim, Ju-Kon Oxford University Press 2010 Journal of experimental botany Vol.61 No.9
<P>Novel constitutive gene promoters are essential components of crop biotechnology. Our analysis of five such promoters, <I>APX</I>, <I>SCP1</I>, <I>PGD1</I>, <I>R1G1B</I>, and <I>EIF5</I>, in transgenic rice plants is reported here. The five promoter regions were linked to the <I>gfp</I> reporter gene and transformed into rice. Using fluorescent microscopy and q-RT-PCR, promoter activities were analysed in comparison with <I>OsCc1</I>, <I>Act1</I>, and <I>ZmUbi1</I>, previously characterized as strong constitutive promoters. The <I>APX</I> and <I>PGD1</I> promoters direct high levels of gene expression in all tissues and stages, producing GFP at levels of up to 1.3% of the total soluble protein. <I>PGD1</I> is particularly active in flowers and mature roots. The <I>R1G1B</I> is active in the whole grain including the embryo, endosperm, and aleurone layer, and thus represents a constitutive promoter with activity in whole seeds that has not been described previously. The <I>ZmUbi1</I> and <I>R1G1B</I> promoters are markedly less active in young roots and mature leaves whilst the <I>APX</I>, <I>PGD1</I>, <I>OsCc1</I>, and <I>Act1</I> promoters are highly active in both vegetative and reproductive tissues. Overall, our results demonstrate that <I>APX</I>, <I>PGD1</I>, and <I>R1G1B</I> are novel gene promoters that are highly active at all stages of plant growth with distinct levels of activity.</P>