국내산 중하(Metapenaeus joyneri) 내 주알레르겐 규명 : 가열 및 소화액에 의한 변화

윤성호,김현아,김현미,최정희,서창희,남동호,김윤근,민경업,박해심 대한알레르기학회 2004 천식 및 알레르기 Vol.24 No.2

Paricalcitol attenuates lipopolysaccharide-induced inflammation and apoptosis in proximal tubular cells through the prostaglandin E₂ receptor EP4

( Yu Ah Hong ),( Keum Jin Yang ),( So Young Jung ),( Yoon Kyung Chang ),( Cheol Whee Park ),( Chul Woo Yang ),( Suk Young Kim ),( Hyeon Seok Hwang ) 대한신장학회 2017 Kidney Research and Clinical Practice Vol.36 No.2

Background: Vitamin D is considered to exert a protective effect on various renal diseases but its underlying molecular mechanism remains poorly understood. This study aimed to determine whether paricalcitol attenuates inflammation and apoptosis during lipopolysaccharide (LPS)-induced renal proximal tubular cell injury through the prostaglandin E₂ (PGE₂) receptor EP4. Methods: Human renal tubular epithelial (HK-2) cells were pretreated with paricalcitol (2 ng/mL) for 1 hour and exposed to LPS (1 μg/mL). The effects of paricalcitol pretreatment in relation to an EP4 blockade using AH-23848 or EP4 small interfering RNA (siRNA) were investigated. Results: The expression of cyclooxygenase-2, PGE₂, and EP4 were significantly increased in LPS-exposed HK-2 cells treated with paricalcitol compared with cells exposed to LPS only. Paricalcitol prevented cell death induced by LPS exposure, and the cotreatment of AH-23848 or EP4 siRNA offset these cell-protective effects. The phosphorylation and nuclear translocation of p65 nuclear factor-kappaB (NF-κB) were decreased and the phosphorylation of Akt was increased in LPS-exposed cells with paricalcitol treatment. AH-23848 or EP4 siRNA inhibited the suppressive effects of paricalcitol on p65 NF-κB nuclear translocation and the activation of Akt. The production of proinflammatory cytokines and the number of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling-positive cells were attenuated by paricalcitol in LPS exposed HK-2 cells. The cotreatment with an EP4 antagonist abolished these anti-inflammatory and antiapoptotic effects. Conclusion: EP4 plays a pivotal role in anti-inflammatory and antiapoptotic effects through Akt and NF-κB signaling after paricalcitol pretreatment in LPS-induced renal proximal tubule cell injury.

출산에 따른 그레이브스병의 재발 및 관련이자

임창훈,최현아,한승석,김해성,이창욱,정호연,한기옥,장학철,박원근,윤현구,한인권 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.2

Background: Pregnancy affects the course of Graves' Disease (GD), and patients who initially maintain euthyroid function into their middle trimester with minimum doses of antithyroid drugs become exacerbated after delivery. Even patients who are completely cured, requiring no treatment during pregnancy, can relapse after delivery. In this study, we examined the postpartum changes in the thyroid functions of patients with GD, and attempted to determine the factors contributing to these changes. Methods: The study subjects were recruited from pregnant women visiting our outpatient clinic for routine prenatal evaluations. 45 women previously diagnosed with GD, who had been treated and cured with hyperthyroidism, and were no longer taking any thyroid medications, were evaluated for 1 year post delivery. Results: Among 45 patients, 20 (44.4%) developed thyroid disorders following delivery. Postpartum thyroiditis (PPT) developed in 8 patients (17.8%), and GD developed in 12 (26.0%). The onset of the PPT disease 3.1 ± 1.4 months following delivery, which was significantly earlier than the 6.7 ± 2.7 months required for the post delivery onset of GD (p=0.003). The TBII values, measured during the thyrotoxic state in each womaen, were negative in women with PPT and positive in 71.4% of women with GD (p=0.030). The duration of treatment for hyperthyroidism prior or pregnancy, the number of recurrences, and the time interval without treatment, were not associated with the development of postpartum thyroid disorders. Whereas, the mean number of past pregnancies for women who developed PPT was 3.9 ± 2.1, and was significantly higher than the 2.2 ± 1.7 for women developing no thyroid dysfunctions (p=0.044). In 13 women their initial onset of GD occurred within one year postpartum, 7 (53.8%) having had a recurrence, which was significantly higher than in women whose disease onset occurred unrelated to delivery (5 of 32 women: 15.6%). Conclusion: Women with GD developed postpartum thyroid dysfunctions in 44.4% of cases. Women whose initial disease onset occurred within one year postpartum had higher recurrences of GD, and women who developed PPT had a history of higher gravidity compared to the euthyroid women postpartum. Therefore, if women with GD develop postpartum thyroid dysfunctions, the diagnosis should be made, and a treatment modality planned, following careful considerations of the patients' past obstetric history, changes in clinical manifestations and the TBII values

소의 대동맥 내피세포에서 산화저밀도 지단백에 의한 아포프토시스에 백일해 독소 및 콜레라 독소가 미치는 영향

손호영(Ho Young Son),강성구(Sung Ku Kang),강무일(Moo Il Kang),차봉연(Bong Youn Cha),이광우(Kwang Woo Lee),장상아(Sang Ah Chang),최윤희(Yoon Hee Choi),문성대(Sung Dae Moon),안유배(Yoo Bae Ahn),손현식(Hyun Sik Son),윤건호(Kun Ho Yoon) 대한내과학회 1999 대한내과학회지 Vol.56 No.5

Objectives : Oxidized LDL is thought to play a key role in atherogenesis. Among their wide variety of biological properties, oxidized LDL injures the endothelium as an early event of atherogenesis. However, the mechanisms by which oxidized LDL injures endothelial cells are not definitely known. In order to evaluate the involvement of GTP-binding protein on the mechanism of apoptosis, we studied the effects of pertussis and cholera toxin on oxidized LDL-induced apoptosis in bovine aortic endothelial cells(BAECs). Methods : Oxidized LDL elicited apoptosis in cultured BAECs as shown by characteristic morphological and biochemical changes. Chromatin condensation and nucleus fragmentation were visualized by using fluorescence microscopy of intact cells staining by acridine orange/ ethidium bromide. DNA fragmentation was quantified by an ELISA with specific antibody for bromodeoxyuridine- labelled DNA fragments and confirmed with DNA ladder formation. Results : Studies using a combination of bacterial toxins which change the function of GTP-binding protein suggest that oxidized LDL-induced apoptosis was regulated by GTP-binding protein. Oxidized LDL-induced apoptosis was not changed by pretreatment of BAECs with pertussis toxin. In contrast, pretreatment with cholera toxin completely prevented the oxidized LDL- induced apoptosis. Conclusion : These results show that oxidized LDL induces apoptosis of BAECs and suggest that cholera toxin-sensitive G-proteins are involved in signal transduction of oxidized LDL-induced apoptosis of BAEC.

1993년에 유행한 무균성 뇌막염 : 서울지역 소아를 대상으로 The study of pediatric patients in Seoul, with the emphasis on the isolation of causative agent

문형남,고태성,박영서,박윤아,홍창희 울산대학교 의과대학 1994 울산의대학술지 Vol.3 No.1

From May to July 1993, we observed an epidemic of aseptic meningitis. We performed clinical and virological studies on 174 children with aseptic meningitis who had been admitted to the Department of Pediatrics, Asan Medical Center, from May to July, 1993 The results were as follows; 1) This epidemic of aseptic meningitis was most prevalent in June 1993. The most of the patients were 3 to 6 years old and male to female ratio was 1.6 : 1. 2) The most patients suffered from fever, vomiting, headache, and abdominal pain was noticed in 36% of patients, characteristically. 3) In 7 cases among 10 randomly sampled cases, enterovirus was cultured. Among them, we performed serotyping in 3 cases and Echovirus 9 was isolated.

Expression of epidermal growth factor receptor and cyclin D1 in pretreatment biopsies as a predictive factor of radiotherapy efficacy in early glottic cancer

Chang, Ah Ram,Wu, Hong-Gyun,Park, Charn Il,Jun, Yoon Kyung,Kim, Chul-Woo Wiley Subscription Services, Inc., A Wiley Company 2008 Head & neck Vol.30 No.7

<B>Background</B><P>To evaluate the prognostic value of the expressions of epidermal growth factor receptor (EGFR) and cyclin D1 in early glottic cancer treated with radiotherapy only.</P><B>Methods</B><P>One hundred fifty-one patients with T1-2, N0 glottic cancer who had been treated with radiotherapy at Seoul National University Hospital since 1992 through 2004. Immunohistochemical staining for EGFR and cyclin D1 were performed on the formalin-fixed paraffin-embedded tissues of 25 patients who developed local recurrence and on the tissues of 25 matched patients free from disease. Patterns and degrees of expression were compared between these 2 groups.</P><B>Results</B><P>High EGFR (p = .047) and high cyclin D1 (p = .040) expressions were both found to be significantly associated with a poor prognosis. No association was found between EGFR and cyclin D1 status (p = .158), but EGFR and cyclin D1 status in combination were found to be significantly associated with local control. The patients with both high EGFR and high cyclin D1 expression had the poorest outcome compared with the others (14 months vs 29 months of median time to progression). Patterns of EGFR and cyclin D1 expression changed after recurrence, but these changes were not found to alter the ultimate prognosis.</P><B>Conclusion</B><P>The molecular biomarkers, EGFR and cyclin D1 have a prognostic significance in early glottic cancer. These markers in combination seem to play an important role in tumor relapse and may be useful for selecting patients with a poor outcome after radiotherapy. © 2008 Wiley Periodicals, Inc. Head Neck, 2008</P>

Streptozotocin으로 유발된 당뇨쥐의 간세포 내 Gi 단백의 양과 기능 변화

옥선명,손현식,홍옥기,이정민,김성래,장상아,윤건호,강무일,차봉연,이광우,손호영,강성구 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.24 No.6

연구배경:당뇨병과 인슐린 작용에 있어 Gi 단백의 역할은 정설이 없는 상태이며, 당뇨병의 유병 기간에 따른 Gi 단백의 변화는 잘 알려져 있지 않다. 본 연구에서는 streptozotocind으로 유발된 인슐린의존성 당뇨쥐의 간세포를 대상으로 당뇨병의 유병 기간에 따른 Gi 단백의 기능적 변화와 Gi 단백의 양적인 변화를 α소단위의 종류에 따라 비교함으로서 인슐린 작용 및 당뇨병의 병인에서 Gi 단백의 역할을 평가하고자 하였다. 방법:Sprague­Dawley계 흰쥐 수컷에 streptozotocin을 정맥 주사하여 당뇨병을 유발시킨 후 1, 2, 3 및 5주에 간조직을 differential ultracentrifugation와 gradient centrifrgation방법으로 전세포분쇄물과 중간분쇄물 및 간세포막으로 분획한 다음 Giα의 양적 변화를 평가하기 위해서 Giα1&2, Giα₃에 대한 항체로 western blot을 실시하였고, 기능적 변화를 평가하기 위해서 pertussis toxin­catalyzed ADP­ribosylation과[35S]­GTPγS binding assay를 실시하였다. 결과:당뇨군과 대조군의 간세포에는 Giα², Giα³이 존재하는데 주로 간세포막에 존재하며, 대조군에 비해 당뇨군의 간세포막의 Giα²와 Giα³의 양이 유의하게 높게 측정되었으나 (p<0.01)당뇨병의 유병 기간 증가에 따른 변화는 없었다. Pertussis toxin­catalyzed ADP­ribosylation와[35S]­GPTγS 결합률을 실시한 결과 대조군에 비해서 당뇨군의 간세포막에서 저하되었으나(p<0.01), 당뇨병의 유병 기간 증가에 따른 변화는 없었다. 결론:인슐린의존성 당뇨쥐의 간세포에서 Gi 단백의 양적 및 기능적 변화가 있으나, 당뇨병의 유병 기간과 관계가 없는 것으로 보아, 인슐린 결핍에 의한 인슐린저항성에 대한 보상 반응으로 생각되며, 이는 인슐린 작용 및 당뇨병에서 Gi 단백이 관여함을 알 수 있었다. Background : The functional and expressional changes of Gi proteins in diabetes have been investigated extensively, no agreement has been reached in the results. Moreover, studies using rats with different diabetic duration, and using α subunits (G_ia) of Gi proteins are lacking in literatures. Thus, we assessed the changes according to the duration of diabetes and examined the expressional changes of G_ia and functional changes of G_i proteins in hepatocytes from streptozotocin-induced diabetic rats. Methods : Male Sprague-Dawley rats were injected with streptozotocin to induce diabetes ; 1, 2, 3 and 5 weeks after teh onset of diabetes, livers from the control and diabetic rats were fractionated into homogenate, interface, and plasma membrane. The levels of G_ia 1&2, G_ia 3 were quantified with western blots in each fraction. The functional changes of Gi proteins were evaluated by performing pertussis to xin-catalyzed ADP-ribosylation and measuring GTPγS binding activity. Results : 1) G_ia 2 and G_ia 3 were present mainly in the plasma membrane of hepatocytes in the diabetic and control rats, but the levels of these subunits were significantly higher in the diabetic rats, but the levels of these subunits were significantly higher in the diabetic rates than in the control rats (p<0.01). The levels of these subunits were not affected by the duration of diabetes. 2) In streptozotocin-induced diabetic rats, the levels of ADP-ribosylation of Gi proteins in liver plasma membranes decreased when pertussis toxin-catalyzed ADP-ribosylation was performed with liver tissues. However, the levels of these proteins were not affected by the duration of diabetes. 3) For the GTPγS binding activity of G_i proteins in liver plasma membranes, the diabetic rats showed significantly less activity than the control rats (p<0.01). However, the activity was not affected by the duration of diabetes. The activity was somewhat restored by the insulin treatment of liver plasma membranes in diabetic rats. Conclusion : These results suggest that the insulin-deficient diabetic state induces the quantitative and functional changes in G_i proteins may be the important compensatory reactions for the insulin resistance occurring in the insulin deficient state (J Kor Diabetes Asso 24:666~677, 2000).

Changes of Seedling Quality of Grafted Watermelon Transplants Grown in Cylindrical Paper Pot by Different Fertilizer Concentrations and Seedling Growing Days

( Se-woong An ),( Tae-cheol Seo ),( Yoon-ah Chang ),( Hee Chun ),( Tae-kyung Kang ),( Sang-hee Lee ) 한국농업기계학회 2019 한국농업기계학회 학술발표논문집 Vol.24 No.1

The objective of this study was to investigate the changes of seedling quality for grafted watermelon transplants grown in cylindrical paper pot by different fertilizer concentrations and seedling growing days. Three kinds of commercial watermelon scions, ‘Goodchance’, ‘Speedkkul’ and ‘Seolgang102’, were grafted onto ‘Sintojwa’ and ‘Dongjanggun’. After completing grafting union formation, the grafted watermelon transplants were cultivated by three different fertilizer levels (0.5, 1.0 and 2.0 standard nutrient solution), and top to root ratio and compactness were measured at 10, 17, 24 and 31 days. Plant height, leaf number, fresh weight and dry weight of grafted watermelon transplants were diverse over the growing days. According to seedling quality, compactness were continuously decreased by 31 growing days, but top to root ratio was continuously increased as growing days increased. While compactness of the grafted seedlings affected by nutrient solutions showed no significant difference at 10 growing days after grafting union formation, there were significant differences at 17, 24 and 31 growing days. In addition, seedling compactness was significantly influenced by scion and rootstock cultivars. For the scion cultivars, compactness was significantly different at 17, 24 and 24 growing days, respectably. For the rootstock cultivars, seedling compactness grafted onto ‘Sintojwa’ was higher than ‘Dongjanggun’rootstock. Results indicated that seedling quality of grafted watermelon grown in cylindrical paper pot was highly affected by the growing days, fertilizer, and scion and rootstock cultivars

한국 농업에서의 유전자원 활용 현황과 금후 전망

김창영 ( Chang Yung Kim ),이정란 ( Jeong Ran Lee ),윤문섭 ( Mun Sup Yoon ),조규택 ( Gyu Taek Cho ),백형진 ( Hyung Jin Baek ),고호철 ( Ho Cheol Ko ),조양희 ( Yang Hee Cho ),전영아 ( Young Ah Jeon ),김정곤 ( Chung Kon Kim ) 한국국제농업개발학회 2010 韓國國際農業開發學會誌 Vol.22 No.3

The application and use of agricultural genetic resources have been reviewed since Korea was freed from the rule of Japanese imperialism in 1945. The whole changes that took place in the Korean agriculture, such as, characteristics of agriculture, application trends of genetic resources, roles of agricultural genebank, and future utilization perspectives of plant genetic resources were reviewed and summarized. The whole changes that took place in the Korean agriculture since the liberation from the Japanese rule were as follows. Before 1960s, Korean agriculture was a subsistence farming system with the landraces, introduction of foreign cultivars, and the beginning stage of domestic breedings. In 1960-1970s, the importance of genetic resources came up because the Korean government had to solve the national shortage of foods by developing and disseminating high-yielding cultivars such as `tongil rice`. In 1980-1990s, four-season-long cultivation of horticultural products were reared and predominantly based on the public needs. Since 2000s, diverse varieties of genetic resources became more important because of the need to cultivate crops with healthy function and high nutritional value. Hence, the interest in the diversity of agricultural genetic resources increased. The Rural Development Administration (RDA) has designated 91 local sub-banks for managing the overall national agricultural genetic resources. RDA installed the seed bank that contain more than 500,000 accessions with high technologies to preserve plant germplasms. Currently, the RDA deposits approximately 160,000 accessions of seeds, and developed and disseminated 2,477 cultivars using these genetic resources. In summary, the major role of national agricultural genebank are as follows: 1) to manage national agricultural genetic resources. 2) to secure diverse genetic resources of current and future values, to conduct multiplication and characteristic assessment of genetic resources, and to utilize genetic resources through safe preservation and distribution service. 3) to conduct a variety of researches such as genetic diversity analyses, develop seed dormancy and preservation tools, assess seed characteristics and multiplication of seeds, develop technology to discover valuable resources using functional genes. 4) to raise the national status for preservation of agricultural genetic resources through international and domestic cooperation. Agricultural genetic resources will be used for the following purposes: to continuously produce competitive cultivars with high quality, to be utilized as raw materials to meet a constant food supply and demands of the general public`s well-being, to be applied to make agri-ecosystem healthy and prosperous, and to be used as a driving force for new growth of industries related to energy, new materials and stuffs.

공유도시 지표설정을 통한 공유도시 수준의 지역적 분포특성 분석

장윤배(Chang, Yoon-Bae),이주아(Lee, Joo-Ah),강성익(Kang, Sung-Ik) 대한국토·도시계획학회 2017 國土計劃 Vol.52 No.3

Recently, sharing economy companies such as Uber and Airbnb has been developed with the rational consumption culture spread and the development of mobile devices such as smart phones. In addition, cities such as Seoul and Amsterdam in the Netherlands are declaring sharing cities and encouraging shared economic activities. However, there have been a lot of practical activities for sharing cities, although there are few theoretical theories that require academic research. In particular, the sharing city is expected to become a basic platform of future city policy in that it emerges due to the development of information and communication technology, the change of society such as low growth, and the necessity of transformation of city policy which was the main focus of development. This study is a basic study to find a way to realize a sharing city in urban policy by deriving a shared city index and analyzing spatial characteristics in the Seoul, Gyeonggi, and Incheon metropolitan area.