갑상선암에서 MAGE 1-6 mRNA를 검출하는MAGE common primer의 의의

남철우,최영식,박요한,이강대 고신대학교의과대학 2006 고신대학교 의과대학 학술지 Vol.21 No.2

Background: MAGE (melanoma antigen gene) was named as cancer/testis specific antigens since their expression is only detected in the testis or cancer cells. Because of their relatively specific expression in the cancer cells, they have been considered as the early diagnosis of several cancers, or the appropriate targets for the specific immunotherapy mediated by cytotoxic T lymphocytes. Therefore, there were many reports about the expression of MAGE genes in various types of malignant tumors, but only a few reports in human thyroid neoplasms. The purpose of this study was to determine whether the MAGE common primers expressed in different histological types of thyroid tumors and to see the clinical usefulness of MAGE common primers on the thyroid tumors. Methods: Fifty-nine patients who underwent thyroidectomy at Kosin Medical Center from January, 2001 to April, 2002 were included in the study. Of the 59 patients 40 were papillary carcinoma, 4 follicular carcinoma, 3 medullary carcinoma, 1 anaplastic carcinoma, 4 nodular hyperplasia, 3 adenomatous goiter, and 4 follicular adenoma. Total cellular mRNA was extracted from those 48 cancer tissues, 11 benign tissues and additional eleven normal control thyroid tissues. Nested reverse transcriptase-polymerase chain reactions (nested RT-PCR) with the MAGE common primers designed to detect MAGE 1-6 genes were employed in this study. With probes confirmed by DNA sequencing. Results: Of those 48 cases of thyroid carcinomas 10 cases (20.8%) were expressed MAGE common primers. In the 40 papillary carcinoma, MAGE common primers were expressed in 17.5%. In the four follicular carcinoma, they were expressed in two cases, and in one case of one anaplatic carcinoma. However, in the three cases of medullary carcinoma, MAGE gene was not expressed. None of 11 benign tumors and 11 normal tissues expressed any of these MAGE common primers. The sensitivity of MAGE common primers in thyroid tumors was 20.8% and the specificity was 100%. Conclusion: These results suggested that MAGE common primers were expressed low in the malignant thyroid tumors but it was not expressed in benign tumors and normal tissues. It is suggested that MAGE common primers can be used for preoperative differential diagnosis of follicular carcinoma from benign follicular adenoma which cannot be made by fine needle aspiration cytology of thyroid tissue. However, further study is needed for clinical application of MAGE common primers expression as tumor markers in the thyroid tumors.

Toxic Effects of Thimerosal on Rat Midbrain Cells

Yo Woo Choi,Seung Jun Kwack 한국실험동물학회 2009 Laboratory Animal Research Vol.25 No.2

Thimerosal has been used as an additive to biologics and vaccines because it is very effective in killing bacteria used in several vaccines and in preventing bacterial contamination. However, the toxic mechanism of thimerosal has not been cleared. The present studies were performed to investigate its cytotoxicity and toxic mechanisms. Thimerosal induced cytotoxicity and inhibited the proliferation and differentiation of the midbrain cells. The reactive oxygen species (ROS) generation was increased by thimerosal treatments. However, antioxicants, such as vitamin E and propyl gallate, protected the oxidative damage on midbrain cells and was recovered midbrain cell proliferation and differentiation. These results suggest that antioxidants provide protection of midbrain cells against cytotoxicity induced by thimerosal by facilitating cellular functions that reduce oxidative stress.

바이너리 실행파일을 위한 정적 프로그램 슬라이서의 설계 및 구현

최준우(Jun Woo Choi),김요셉(Yo Seph Kim),최철순(Chul Soon Choi),이정민(Jeong Min Lee),안우현(Woo Hyun Ahn) 한국정보과학회 2011 한국정보과학회 학술발표논문집 Vol.38 No.2B

프로그램 슬라이싱(program slicing)이란 프로그램 내의 관심 있는 한 지점으로부터 특정 변수에 직 간접적으로 영향을 주는 모든 문장들을 추출하는 기법이다. Weiser가 처음 프로그램 슬라이싱의 개념을 제안한 이후 현재까지 다양한 기법을 이용한 프로그램 슬라이싱 기법이 연구되었다. 이에 따라 다양한 프로그램 슬라이서(program slicer)가 구현되어 있다. 하지만 이들 대부분은 원본 소스코드에 대한 슬라이싱만을 수행할 뿐 컴파일된 바이너리는 처리할 수가 없다. 컴파일된 바이너리 코드의 경우 함수 호출에 따른 콜 스택의 관리 레지스터의 사용 간접 참조에 의한 분기 등 문제로 인해 슬라이서의 구현에 어려움이 있다. 따라서 본 논문에서는 스택변경 모듈과 Control Flow Graph를 이용하여 바이너리 실행 파일의 코드에 대한 정적 바이너리 프로그램 슬라이서를 구현 하였다.

Sirolimus- vs. paclitaxel-eluting stents for the treatment of unprotected left main coronary artery stenosis: Complete 2-year follow-up of a two-center registry

Park, Kyung Woo,Kang, Si-Hyuck,Park, Keun-Ho,Choi, Dong-Hyun,Lee, Hae-Young,Kang, Hyun-Jae,Cho, Young-Seok,Yeon, Tae-Jin,Chung, Woo-Young,Koo, Bon-Kwon,Chae, In-Ho,Choi, Dong-Ju,Oh, Byung-Hee,Park, Yo Elsevier 2011 INTERNATIONAL JOURNAL OF CARDIOLOGY Vol.151 No.1

<P><B>Abstract</B></P><P><B>Background</B></P><P>The feasibility of percutaneous coronary intervention (PCI) using drug-eluting stents and its comparability with bypass surgery in treatment of unprotected left main coronary artery (LMCA) stenosis has been shown previously. We compared the mid-to long-term outcome between sirolimus-(SES) vs. paclitaxel-eluting stents (PES) in an all-comer analysis that included all patients with unprotected LMCA stenosis who underwent PCI with SES or PES.</P><P><B>Methods</B></P><P>From March 2003 and June 2007, 196 patients underwent PCI with SES or PES for unprotected LMCA stenosis at Seoul National University Main or Bundang Hospital; SES was implanted in 141 patients and PES in 55 patients. The baseline clinical and procedural characteristics were mostly similar between the SES and PES group.</P><P><B>Results</B></P><P>After 2years of follow-up, there were no differences in the rate of cardiac death (9.1% vs. 8.5%) and nonfatal MI (5.5% vs. 2.8%) between the two groups. However, the risk of repeat revascularization tended to be lower in the SES group compared with the PES group [TLR, 9.9% vs. 20.0% (<I>P</I>=0.06); TVR, 17.7% vs. 30.9% (<I>P</I>=0.05)], which did not reach statistical significance. The rate of stent thrombosis (ST) was also similar between the two groups (3.6% vs. 2.1% for definite ST, 3.6% vs. 2.8% for definite+probable ST).</P><P><B>Conclusions</B></P><P>In all-comers undergoing first generation DES implantation for unprotected LMCA stenosis, PES and SES showed comparable 2-year clinical results regarding hard endpoints and major adverse cardiac events.</P>

Vegetation Classification Using Seasonal Variation MODIS Data

Choi, Hyun-Ah,Lee, Woo-Kyun,Son, Yo-Whan,Kojima, Toshiharu,Muraoka, Hiroyuki The Korean Society of Remote Sensing 2010 大韓遠隔探査學會誌 Vol.26 No.6

The role of remote sensing in phenological studies is increasingly regarded as a key in understanding large area seasonal phenomena. This paper describes the application of Moderate Resolution Imaging Spectroradiometer (MODIS) time series data for vegetation classification using seasonal variation patterns. The vegetation seasonal variation phase of Seoul and provinces in Korea was inferred using 8 day composite MODIS NDVI (Normalized Difference Vegetation Index) dataset of 2006. The seasonal vegetation classification approach is performed with reclassification of 4 categories as urban, crop land, broad-leaf and needle-leaf forest area. The BISE (Best Index Slope Extraction) filtering algorithm was applied for a smoothing processing of MODIS NDVI time series data and fuzzy classification method was used for vegetation classification. The overall accuracy of classification was 77.5% and the kappa coefficient was 0.61%, thus suggesting overall high classification accuracy.

Quantum dots based light emitting diodes

Yo-Han SUH,Jung Kyu KIM,Sukang BAE,Jin-Woo CHOI,Jong Hyeok PARK,Jongnam PARK,Chang-Lyoul LEE 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8

人蔘 優良系統의 生長 및 成分 比較

Woo Saeng Kwon(權宇生),Chan Moon Chung(鄭燦文),Yo Tae Kim(金鐃,泰),Kwang Tae Choi(崔光泰) 한국육종학회 1991 한국육종학회지 Vol.23 No.3

This study was carried out to clarify the characteristics of 5 superior lines. selected for development of varieties of ginseng(Panax ginseng C. A. Meyer) The growth of 2-year-old superior lines was better than that of Jakyungjong and KG 102 line among them showed the most vigorous growth. And 4-year-old superior lines also showed vigorous growth in aerial parts. Especially, KG 102 line showed short and multiple stems. Crude saponin and ginsenoside contents were found in a high yield in both taproot and lateral root of KG 103 line and ginsenoside contents were also found in a high yield in lateral root of KG 104 line. Ginsenoside -Rb₁ and -Rg₁ were found in a high yield in the taproot of KG 101,102,103 and 104 lines but in a low yield in Jakyungjong and Hwangsookjong. Ginsenosides -Ra and -Ro were found in a high yield in the taproot of KG 101 line but were not found in KG 104 line. Hwangsookjong showed the low yield of crude saponin and ginsenosides but ginsenoside pattern was uniform. Anthocyanin contents were found in a high yield in aerial parts of KG 103 line.

Incarcerated umbilical hernia with small bowel obstruction in a continuous ambulatory peritoneal dialysis patient

Yo-han Jeong,Jun-young Do,Mun-ju Hwang,Min-Jung Kim,Min Geun Gu,Byung-sam Park,Jung-eun Choi,Tae-woo Kim 영남대학교 의과대학 2014 Yeungnam University Journal of Medicine Vol.31 No.1

Patients treated with peritoneal dialysis have increased intra-abdominal pressure and a high prevalence of abdominal wall complications. Hernias can lead to significant morbidity in patients on peritoneal dialysis. Hernias are clinically important because of the risk of incarceration, strangulation and subsequent bowel obstruction, rupture, and peritonitis. In this paper, a case of incarcerated umbilical hernia with small bowel obstruction in a continuous ambulatory peritoneal dialysis (CAPD) patient is reported. The small bowel obstruction improved after herniorrhaphy, and the peritoneal dialysis was resumed 2 weeks after the herniorrhaphy. The patient had been undergoing CAPD without technical failure until the 2 months follow-up after the herniorrhaphy. This case shows that early detection of incarcerated umbilical hernia and herniorrhaphy can prevent resection of a strangulated small bowel so that it can remain on CAPD without post-operative technical failure. Umbilical hernias should be carefully observed and intestinal obstruction should be considered when a CAPD patient with an umbilical hernia has abdominal pain.

高麗人蔘의 紫莖種과 黃熟種 紅蔘品質 特性比較

Woo Saeng Kwon(權宇生),Jang Ho Lee(李璋浩),Je Yong Kang(姜齊瑢),Yo Tae Kim(金鐃,泰),Kwang-Tae Choi(崔光泰) 한국육종학회 1994 한국육종학회지 Vol.26 No.4

In order to clarify the characteristics of red ginseng quality of Jakyung-jong(violetstem variant) and Hwangsook-jong(yellow-berry variant) in Panax ginseng, the red ginseng grades were classified. The ratio of Chun-sam(Heaven grade) was higher in Hangsook-jong than in jakyung-jong, and Chun-sam was also degraded to poor quality, jee-sam and Yang-sam, by second classification. Poor quality of red ginseng was caused by inside white, inside cavity, cracking, poor rhizome, and so on. Especially, the ratio of inside white showed the highest among them, and was higher in Jakyung-jong than in Hwangsook-jong. And it was found that the high quality red ginseng was larger in Hwangsook-jong than in Jakyung-jong.

토끼 음경 해면체 평활근에서 SS-cream의 효과

최영득,신종성,정우식,최형기,하종식,박영요 이화여자대학교 생명과학연구소 1995 생명과학연구논문집 Vol.6 No.-

SS-cream is a complex mixture containing 9 oriental herbs for treating the premature ejaculation, which is based on the traditional chinese royal herb remedy. Clinically SS-cream has been effective in the treatment of premature ejaculation and in some patients, potentiating effect of their erectile capacity was noted probably due to its combined activity of several vasoactive priciples. Therefore, we investigated the pharmacological action of SS-cream in the isolated rabbit corporal smooth muscle. Strips of rabbit corpus cavernosum were isolated and mounted in 10ml organ chambers to measure isometric tension. Muscle strips treated with increasing concentrations of SS-cream(0.05mg/ml to 0.3mg/ml) showed initial rapid contraction followed by slow gradual relaxation. Muscle strips submaximally precontracted with phenylephrine(PHE:5×10^-6M) and treated with increasing concentrations of SS-cream(from 0.05mg/ml to 0.2mg/ml) showed also initial rapid contraction above the precontracted level and thereafter, relaxed to the basal level at the dose larger than 2mg/ml of SS-cream. Relaxations of muscle strips to SS-cream were not inhibited even partially by endothelial disruption or by pretreatment with pyrogallol or methylene blue. Pretreatment of muscle strips with SS-cream caused concentration dependent inhibition of PHE(5×10^-6M) induced contraction. With these results we can conclude that SS-cream has dual action, initial rapid contractile effect which is mediated by adrenergic alpha receptor simulation, and delayed nonspecific relaxing effect which is not mediated by EDRF or nitric oxide.