SSR 마커를 이용한 복숭아 품종의 유전적 다양성 분석

홍지화(Jee-Hwa Hong),이승인(Seung-In Yi),권용삼9Yong-Sham Kwon),김영(Young Kim),최근진(Keun-Jin Choi) 한국육종학회 2013 한국육종학회지 Vol.45 No.3

The objective of this study was to evaluate genetic diversity in 72 major peach varieties by using SSR markers. A set of 189 SSR primer pairs was screened and 74 primer pairs showed polymorphism in 9 varieties. Twenty primer pairs out of 74 primer pairs showed clear band pattern and repetitive reproducibility. The relationship between 20 markers genotypes and 72 varieties was analyzed. A total of 71 polymorphic amplified fragments were obtained by using 20 SSR markers. Two to nine SSR alleles were detected for each locus with an average of 3.6 alleles per locus. Average polymorphism information content(PIC) was 0.523, ranging from 0.246 to 0.771. A total of 71 marker loci were used to calculate Jaccard’s distance coefficients for cluster analysis using UPGMA. Clustering group was largely divided 2 groups according to absence or presence of pubescence on the fruit surface and genetic distance of cluster ranging from 0.39 to 1.00. Analysis of genetic diversity revealed that these 20 SSR marker sets discriminated a total of 68 varieties except for 4 mutant varieties among 72 varieties. These SSR markers will be utilized as molecular evidence in variety identification of peach.

Disorders of Liver : Posttransplantation Lymphoproliferative Disorder After Liver Transplantation In Pediatric Patients

( Jung Hwa Lee ),( Nam Joon Yi ),( Kyung Suk Suh ),( Kuhn Uk Lee ),( Gyeong Hoon Kang ),( Jeong Kee Seo ),( Jea Sung Ko ),( Ju Young Chang ),( Hye Ran Yang ),( Jeong Ok Shim ),( Jae Joon Han ),( Jee Y 대한소화기학회 2007 SIDDS Vol.9 No.-

Background/Aims: Posttransplantation lymphoproliferative disorder (PTLD) is a serious disease that has been defined as uncontrolled proliferation of lymphocytes in a context of posttransplant immunosuppression. Because it may progress to malignancy, PTLD is a life-threatening complication of organ transplantation in children. The aim of this study is to evaluate the clinical features, diagnosis, treatment and outcome of PTLD after liver transplantation in Korean children. Methods: From January 1988 to December 2006, 7 of 121 pediatric patients who underwent liver transplantation were diagnosed with PTLD. We retrospectively analyzed the age at transplantation, time of presentation after transplantation, clinical manifestations, histological findings, results of EBV assessments, managements and outcomes of PTLD. Results: The incidence of PTLD in liver transplant pediatric recipients was 5.8%. The mean (SD) age of patients at the time of liver transplantation was 8.57±4.65 months (range 4 to 18 months). PTLD were diagnosed less than 8 months after transplantation in five patients, at 41 months in one, and at 61 months in one. The common clinical presentations were persistent fever, diarrhea and hematochezia. Six of seven patients were found with EBV-positive. Histologic findings showed ``early lesion`` in two, polymorphic in two, and monomorphic in three patients. Burkitt lymphoma and lymphoblastic lymphoma were found in two of three monomorphic patients. All patients were treated with reduction of immunosuppression and infusion of gancyelovir. Rituximab was added in three patients. PTLD were successfully managed in all patients except one who died of sepsis during chemotherapy. Conclusions: PTLD is a fatal disease in liver transplant pediatric recipients. Early detection through the quantitative assay of EBV or gastrointestinal endoscopy, and adequate treatment such as reduction of immunosuppressant, antiviral agent and anti-CD 20 antibody therapy are important in the favorable outcome of PTLD.

수도권 지역에서의 고해상도 지형과 지면피복자료에 따른 수치모의 민감도 실험

박성화(Sung-Hwa Park),지준범(Joon-Bum Jee),이채연(Chaeyeon Yi) 한국기상학회 2015 대기 Vol.25 No.2

The objective of this study is to evaluate the impact of the high resolution topographies and landuses data on simulated meteorological variables (wind speed at 10 m, temperature at 2 m and relative humidity at 2 m) in WRF. We compare the results with WRF simulation using each resolution of the topographies and landuses, and with 37 AWS observation data on the Seoul metropolitan regions. According to results of using high-resolution topography, WRF model gives better topographical expression over domain. And we can separate more detail (Low intensity residential, high intensity residential, industrial or commercial) using high resolution landuses data. The result shows that simulated temperature and wind speed are generally higher than AWS observation data. However, simulation trend with temperature, wind speed, and relative humidity are similar to observation data. The reason for that is that the high precipitation event occurred in CASE 1 and 2. Temperature have correlation of 0.43~0.47 and standard deviation of 2.12~2.28 ℃ in CASE 1, while correlation of more than 0.8 and standard deviation of 3.05~3.18 m s<SUP>?1</SUP> in CASE 2. In case of wind speed, correlation have lower than 0.5 and Standard Deviation of 1.88~2.34 m s<SUP>?1</SUP> in CASE 1 and 2. In statistical analysis shows that using highest resolution (U01) results are more close to the AWS observation data. It can be concluded that the topographies and landuses are important factor that affect model simulation. However, the tendency to always use high resolution topographies and landuses data appears to be unjustified, and optimal solution depends on the combination of scale effect and mechanisms of dynamic models.

16. 폴리에틸렌옥사이드를 이용한 세파트리진 - 프로필렌글리콜레이트의 서방성 정제의 제조 및 평가

이언형(Eon Hyoung Yi),이계원(Gye Won Lee),이종화(Jong Hwa Lee),김동출(Dong Chool Kim),지웅길(Ung Kil Jee) 한국약제학회 1997 한국약제학회 총회 및 학술대회 요지집 Vol.- No.27

N/A

고농도 황산염 이온이 함께 존재하는 경우의 염소이온 침투특성에 관한 실험 연구

오병환(Byung-Hwan Oh),정상화(Sang-Hwa Jung),강의영(Yi Rong Jiang),김지상(Jee-Sang Kim) 한국콘크리트학회 2003 콘크리트학회논문집 Vol.15 No.2

Plasma leptin concentrations are greater in type II diabetic patients and stimulate monocyte chemotactic peptide-1 synthesis via the mitogen-activated protein kinase/extracellular signal-regulated kinase pathway

( Jin Joo Cha ),( Young Youl Hyun ),( Yi Hwa Jee ),( Mi Jin Lee ),( Kum Hyun Han ),( Young Sun Kang ),( Sang Youb Han ),( Dae Ryong Cha ) 대한신장학회 2012 Kidney Research and Clinical Practice Vol.31 No.3

Background: Leptin is an adipokine that is recently reported to be a biomarker of systemic inflammation. Although atherosclerosis causes cardiovascular diseases, it is not clear whether leptin contributes to the acceleration of this process. In this study, we investigated whether alterations of plasma leptin levels were related to diabetic nephropathy and systemic inflammation. In addition, we examined the physiologic action of leptin in cultured vascular smooth muscle cells (VSMCs). Methods: A total of 126 type 2 diabetic participants and 37 healthy controls were studied. The diabetic participants were divided into three groups according to stage of nephropathy. We investigated whether leptin induced monocyte chemo- tactic peptide-1 (MCP-1) synthesis through the mitogen-activated protein kinase (MAPK) pathway using cultured VSMCs. Results: Plasma leptin concentrations were significantly higher in the diabetic group than in the controls. Plasma leptin levels were positively correlated with body mass index, fasting and postprandial blood glucose, hemoglobin A1c, total cholesterol, urinary albumin excretion, high-sensitivity C-reactive protein (hsCRP), and MCP-1 plasma levels, and negatively correlated with creatinine clearance values. In cultured VSMCs, leptin increased MCP-1 production in a dose-dependent manner, and this stimulating effect of leptin on MCP-1 expression was reversed by the MAPK (MEK) inhibitor PD98059. In addition, leptin stimulated the phosphorylation of MEK, extracellular signal-regulated kinase, and E26-like transcription factor, which are components of the MAPK pathway. Conclusions: Overall, these findings suggest that activation of leptin synthesis may promote MCP-1 activation in a diabetic environment via the MAPK pathway in VSMCs and that it possibly contributes to the acceleration of atherosclerosis.

Role of aldosterone in diabetic nephropathy

CHA, DAE RYONG,KANG, YOUNG SUN,HAN, SANG YOUB,JEE, YI HWA,HAN, KUM HYUN,KIM, HYOUNG KYU,HAN, JEE YOUNG,KIM, YOUNG SIK Blackwell Science Pty 2005 Nephrology Vol.10 No.suppl2

<P>SUMMARY: </P><P>In the last 10 years, many studies have focused on the non-classical action of aldosterone. One of the most important new aspects of aldosterone is its pathogenic role as proinflammatory and profibrotic molecules. It has been reported that aldosterone induces myocardial fibrosis and vascular inflammation through up-regulation of various proinflammatory and profibrotic cytokines. We investigated the effect of aldosterone and spironolactone, which is a non-selective mineralocorticoid receptor antagonist, on monocyte chemoattractant peptide (MCP-1) and collagen synthesis in cultured mesangial and tubular epithelial cells. In addition, to evaluate the effect of spironolactone on diabetic nephropathy, we used Otsuka Long-Evans Tokushima Fatty (OLETF) rats which are known type 2 diabetic animal models. Spironolactone treatment did not induce any significant change in blood glucose levels and blood pressure. However, spironolactone therapy significantly inhibited urinary albumin and MCP-1 excretion. Spironolactone treatment also suppressed renal mRNA expression for MCP-1, macrophage migration inhibitory factor (MIF) as well as intrarenal protein synthesis for ED-1 and MIF. Morphologically, spironolactone treatment significantly prevented glomerulosclerosis, collagen deposition and connective tissue growth factor (CTGF) expression in diabetic rats. In cultured cell experiments, aldosterone directly increased the MCP-1, collagen secretion and spironolactone treatment abolished aldosterone-induced MCP-1 and collagen synthesis. Surprisingly, aldosterone treatment did not induce any significant change in TGF&bgr;1 gene transcription. Finally, we found that NF-kB activity was increased after stimulation with aldosterone and spironolactone therapy inhibited their activation. In addition, prior treatment with pyrrolidine dithiocarbamate (PDTC), which is a NF-KB inhibitor, inhibited aldosterone-induced MCP-1 protein secretion. These results suggest that aldosterone blockade could play a role in preventing the progression of diabetic nephropathy via anti-inflammatory and antifibrotic mechanisms.</P>

Angiotensin II stimulates the synthesis of vascular endothelial growth factor through the p38 mitogen activated protein kinase pathway in cultured mouse podocytes.

Kang, Young Sun,Park, Yun Gyu,Kim, Bo Kyung,Han, Sang Youb,Jee, Yi Hwa,Han, Kum Hyun,Lee, Mi Hwa,Song, Hye Kyoung,Cha, Dae Ryong,Kang, Shin Wook,Han, Dae Suk Journal of Endocrinology (Ltd. by Guarantee) 2006 Journal of molecular endocrinology Vol.36 No.2

<P>Angiotensin II (Ang-II) and vascular endothelial growth factor (VEGF) have an important role in the pathogenesis of diabetic nephropathy, but the signaling cascade of VEGF regulation in response to Ang-II in podocytes is largely unknown. In these experiments, we looked at the effect of Ang-II on the production of VEGF, and investigated whether VEGF production depends on the p38 mitogen activated protein kinase (MAPK) pathway in cultured mouse podocytes. Incubation of podocytes with Ang-II induced a rapid increase in VEGF mRNA expression and protein synthesis as well as its transcriptional activity in an Ang-II dose-dependent manner. To further define the role of angiotensin type 1 (AT1) and type 2 (AT2) receptors involved in Ang-II-mediated VEGF synthesis, the effects of selective AT1 and AT2 receptor antagonists were evaluated. Prior treatment with losartan significantly inhibited VEGF mRNA and protein synthesis induced by Ang-II, which suggests that the AT1 receptor is involved in Ang-II-mediated VEGF synthesis. Furthermore, stimulation of the cells with Ang-II increased both phosphorylation of p38 MAPK and MAP kinase kinase 3/6 (MKK3/6). Additionally, Ang-II enhanced the DNA binding activity to cAMP response element binding protein (CREB) and phosphorylation of CREB. In addition, to investigate the role of p38 MAPK in Ang-II-induced VEGF synthesis, podocytes were pretreated with or without the p38 MAPK inhibitor, SB203580 for 24 h to observe whether Ang-II-mediated VEGF synthesis was inhibited by blocking p38 MAPK. The addition of SB203580 led to a marked inhibition of the increased VEGF mRNA and protein production induced by Ang-II in a dose-dependent manner. Taken together, these results suggest that Ang-II stimulates the synthesis of VEGF in podocytes and the production of VEGF induced by Ang-II is mediated, in part, through the activation of the p38 MAPK pathway.</P>

MODIS NDVI와 기상자료를 이용한 우리나라 벼 수량 추정

홍석영 ( Suk Young Hong ),허지나 ( Ji Na Hui ),안중배 ( Joong Bae Ahn ),이지민 ( Jee Min Lee ),민병걸 ( Byoung Keol Min ),이충근 ( Chung Kuen Lee ),김이현 ( Yi Hyun Kim ),이경도 ( Kyung Do Lee ),김선화 ( Sun Hwa Kim ),김건엽 ( Gun 대한원격탐사학회 2012 大韓遠隔探査學會誌 Vol.28 No.5

본 연구에서는 농경지의 면적 산정과 다양한 작물의 생산량 추정을 위해 많이 활용되고 있는 MODIS 영상과 기상자료를 이용하여 우리나라의 벼 수량을 추정해보고자 하였다. Terra와 Aqua 위성으로 부터 얻어진 시계열 MODIS NDVI 자료를 구축하고 수량과의 상관성이 높은 시기를 선택하여 기상자료와 함께 다중회귀 모형 기반으로 우리나라 벼 수량을 추정하였다. 2002년부터 2010년까지 MODIS 위성 식생지수 NDVI와 기상자료(일조시간 및 일사량)를 이용하여 우리나라 벼 수량 추정을 위한 다중 회귀모형을 작성하였다. MODIS NDVI와 관측소 기상자료 1을 이용한 모형으로 2011년 쌀 수량 추정한 결과 494.6 kg 10a-1로 통계청이 발표한 최종 수량과는 1.1 kg 10a-1 차이가 나는 것으로 나타났다. MODIS NDVI와 관측소 기상자료 2를 이용한 모형으로 2011년 쌀 수량 추정한 결과 509.7 kg 10a-1로 통계청이 발표한 최종 수량과 14.1 kg 10a-1 차이가 나는 것으로 나타났다. 2002년부터 2011년까지의 우리나라 쌀 수량 분포도를 작성하였다. The objective of this study was to estimate rice yield in Korea using satellite and meteorological data such as sunshine hours or solar radiation, and rainfall. Terra and Aqua MODIS (The MOderate Resolution Imaging Spectroradiometer) products; MOD13 and MYD13 for NDVI and EVI, MOD15 and MYD15 for LAI, respectively from a NASA web site were used. Relations of NDVI, EVI, and LAI obtained in July and August from 2000 to 2011 with rice yield were investigated to find informative days for rice yield estimation. Weather data of rainfall and sunshine hours (climate data 1) or solar radiation (climate data 2) were selected to correlate rice yield. Aqua NDVI at DOY 233 was chosen to represent maximum vegetative growth of rice canopy. Sunshine hours and solar radiation during rice ripening stage were selected to represent climate condition, Multiple regression based on MODIS NDVI and sunshine hours or solar radiation were conducted to estimate rice yields in Korea. The results showed rice yield of 494.6 kg 10a-1 and 509.7 kg 10a-1 in 2011, respectively and the difference from statistics were 1.1 kg 10a-1 and 14.1 kg 10a-1, respectively. Rice yield distributions from 2002 to 2011 were presented to show spatial variability in the country.