돼지 卵子의 透明帶에 대한 單一클론抗體生産과 그 特性에 關한 硏究

金鐘培,劉永春,金昌圭,權五中,鄭盛元,鄭吉生 건국대학교 동물자원연구센터 1991 動物資源硏究誌 Vol.16 No.-

本 試驗은 單一클론抗體의 강한 特異性과 抗體性質의 不變性을 이용하여 發生學的 側面에서 哺乳動物 卵子의 透明帶의 機能과 構造를 이해하고, 또한 種特異的인 精子 受容體의 存在 및 生化學的 構造를 규명하기 위한 기초연구로서, 돼지 卵子의 透明帶를 免疫原으로 하여 BABL/c 생쥐로부터 單一클론抗體를 생산하고 그 특성을 구명하였던 바 다음과 같은 결과를 얻었다. 1) 3마리의 BABL/c 생쥐(YⅠ, YⅡ, ZI)에 돼지卵子의 透明帶를 免疫化하고, 複合抗體 生成을 확인한 후 생쥐의 脾臟細胞와 Myeloma(SP2/O-Ag14)를 polyethylene glycol를 融合을 실시한 결과 각각 25.8%, 54.5% 그리고 59.7%의 融合效率을 나타내었으며, ELISQ에 의해 陽性反應을 조사한 결과 각각 17.3%, 32.6% 그리고 6.2% 陽性反應 效率을 나타내었다. 2) YI에서 강한 陽性反應을 보인 6개의 well에 대한 cloning을 실시하고 抗體檢證을 행한 결과 20.8% ∼ 48.4%의 Cloning效率과 54.6% ∼ 82%의 陽性反應 效率을 나타내었다. 3) 강한 陽性反應을 나타낸 항체에 대해 sandwich ELISA法에 의해 isotype을 決定하였던바 2E93C(YⅠ), 3E83E7(YⅠ), 4E3(YⅡ)각각 IgG₂b, IgG₂a, IgM으로 확인되었다. 4) Isotype이 決定된 2E93C9(YⅠ), 3E84E7(YⅠ), 4E3(YⅡ)의 세포를 생쥐의 腹腔에 주사하여 얻은 腹水를 indirect ELISA法에 의해 titer를 決定한 결과 모두 1:400,000 이상의 높은 titer를 나타내었다. 5) 處理區로서 單一클론抗體의 腹水와 對照區로서 normal mouse serum이 각각 2%씩 함유된 배양액속에서 난자를 배양한 후 顯徵鏡下에서 관찰했을 때 對照區에서 배양된 난자의 표면은 정상적인 형태를 나타냈으나 處理區에서 배양된 卵子는 표면에 뚜렷한 沈澱層을 형성하였다. 6) 處理區와 對照區 卵子를 Rabbit anti-mouse IgG-FITC가 1% 함유된 배양액속에서 배양하고 洗滌한 후 螢光顯徵鏡下에서 관찰한 바 處理區의 卵子는 透明帶 주위에서 螢光이 나타났으나, 對照區에서는 나타나지 않았다. This study was carried out ot produce and characterize monoclonal antibodies against porcine zona pellucida, and undertaken as a basic study to develop immunocontraceptive vaccine and to investigate the function of zana pellucida in early fertilization process. The results obtained were summarized as follows: 1. Spleen cells of three BALB/C mice(YⅠ, YⅡ and ZI) which were immunized with porcine zona pelucida were fused with myeloma cells(SP2/O-Ag14) by polyethylene glycol. At the result of fusion, fusion efficiency was 25.8 , 54.5% and 59.7% and positive efficiency 17.3%, 32.6% and 6.2%, respectively. 2. Cloning efficiency was shown to be from 20.8% to 48.4% and positive efficiency of them were 54.6% to 82%. 3. Sub-isotypes of three strong positive antibodies, 2E93C(YⅠ), 3E83E7(YⅠ) and 4E3(YⅡ) were determined by sandwich ELISA method and shown to be as IgG2b, IgH2a and IgM, respectively. 4. The titers of three ascitic fluids containing antibodies, 2E93C9(YⅠ), 3E84E7(YⅠ) and 4E3(YⅡ) were determined by indirect ELISA and all of them showed above 1:400,000. 5. The layer of precipitate was observed on the zona incubated with medium containing 2% ascitic fluid of monoclonal antibody while the eggs treated with 2% normal mouse serum did not. 6. Porcine eggs incubated with medium containing 2% ascitic fluid of monoclonal antibody and followed by subsequent incubation with Rabbit anti-mouse IgG-FITC conjugate showed strong fluorescent light on the zona surface while the zona of normal mouse serum-treated eggs did not show fluorescence.

Decentering Korean Identity with Diasporic Art: A Conversation with Y. David Chung

Y. David Chung 고려대학교 민족문화연구원 2018 Cross-Currents Vol.0 No.29

In conversation with Hijoo Son, Phillips Academy, and Jooyeon Rhee, The Hebrew University of Jerusalem Y. David Chung is an artist and filmmaker known for his film and video works, installations, performances, drawings, prints, and public artworks. This interview combines two conversations on the concept of Korean identity and diasporic art: one that took place in 2008, after Chung finished filming his documentary Koryo Saram: The Unreliable People, co-directed with Matt Dibble, and the other in 2018. Hijoo Son and Jooyeon Rhee jointly designed the questions, interviewed Professor Chung, and redacted the transcript into its present form.

Comment on 'Enhancement in hardness and transmittance of ZnS via SiO₂/Y₂O₃ multilayer'

Chung, Y.D.,Cho, D.H.,Kim, S.B.,Han, W.S. Elsevier Sequoia 2016 Journal of Alloys and Compounds Vol.664 No.-

In a recent paper [1], Huayu Zhang et al. reported data on the enhancement in hardness and transmittance of ZnS via SiO<SUB>2</SUB>/Y<SUB>2</SUB>O<SUB>3</SUB> multilayer. In particular, the authors discussed the chemical state of sputtered Y<SUB>2</SUB>O<SUB>3</SUB> on SiO<SUB>2</SUB>/ZnS measured by X-ray photoelectron spectroscopy. We suggested that the split spectrum of Y 3d doublet should be decomposed into the sum of the doublet and the exact notation of O 1s level should be applied. The O 1s of 528.8 eV could also be assigned to Y<SUB>2</SUB>O<SUB>3</SUB> and the O 1s of 531.5 eV could be assigned to Si related compound.

F-free Y & Cu 전구용액 적용에 의한 YBCO coated conductors의 MOD 공정 개선

김영국,유재무,정국채,고재웅,조윤숙,허은옥,Kim, Y.K.,Yoo, J.M.,Chung, K.C.,Ko, J.W.,Cho, Y.S.,Heo, E.O. 한국초전도학회 2006 Progress in superconductivity Vol.8 No.1

Total Fluorine content in the precursor solution for MOD processing of YBCO coated conductors can be significantly reduced by synthesizing precursor solution with F-free Y & Cu precursor and Barium trifluoroacetate(TFA). It was shown that crack-free and uniform precursor films were formed after calcinations in humidified oxygen atmosphere. Less than 2 hours are required to finish the calcination process, and XRD measurement shows that $BaF_2,\;CuO,\;and\;Y_2O_3$ are major constituent of calcined precursor films. Film thickness after calcination was measured to be ${\sim}2.8$ um by applying slot-die coating method. In particular, addition of Samarium shows critical current of Ic=226 A/cm-w($Jc=3.4\;MA/cm^2$). Also discussed are recent developments in the reel-to-reel processing using F-free Y & Cu precursor solutions. It is shown that uniform and fast processing route to YBCO coated conductor with high Ic can be provided by employing F-free Y & Cu precursor solutions in MOD process.

표면탄성파 소자 응용을 위한 $LiTaO_3$ 단결정 성장

정대식,노용래,박병학,김유성,Chung, D.S.,Roh, Y.R.,Park, B.H.,Kim, Y.S. 한국결정성장학회 1994 한국결정성장학회지 Vol.4 No.2

표면탄성파 소자응요을 위하여 완전용융조성, 48.65 mole % $Li_2O에서 LiTaO_3(LT)$ 단결정(Y, X-axis)을 육성하였다. 육성된 $LiTaO_3(LT)$ 단결정의 SAW 소자성능을 확인하기 위하여 photolithography를 거쳐 기본적인 SAW filter를 제작하였다. Yamaju LT Y-cut 단결정 기판과 본 연구에서 제작된 RIST LT Y-cut 단결정 기판에서의 SAW특성결과를 비교하여 성능을 결정하였다. Yamaju LT 기판에서보다 본 연구에서 육성된 LT 단결정 기판에서 우수한 SAW 성능을 보였다. $LiTaO_3 (LT)$ single crystals (Y, X-axis) were grown from the congruent composition, 48.65 mole %, $Li_2O$ for SAW (Surface Acoustic Wave) applications. Basic SAW filters were fabricated on the RIST prepared LT wafers (Y-cut) using phtolithography. SAW filter performance was evaluated. The results were compared of the SAW characteristics between RIST prepared LT wafer (Y-cut) and commercial Yamaju wafer (Y-cut). The SAW filter prepared on the RIST grown LT wafer was shown better SAW performances than that of Yamaju wafer.

200 GeV/핵자 유황이온과 핵건판핵의 충돌에 의해 생성된 헬륨 파쇄핵의 극한파쇄 연구

김동철,송진섭,윤천실,정성헌,박인곤,김종오,김철수,김태연,이승희,조재희,천병구,김재률,김준원,김태익,박명렬,장한일,임인택 慶尙大學校 기초과학연구소 1992 基礎科學硏究所報 Vol.8 No.-

고에너지 중이온 원자핵과 핵건판의 충돌에서, 200GeV/핵자 유황이온에 의해 생성된 파쇄 헬륨핵(Z=2)의 실험실계의 방출각 분포는 표적핵에 무관한 회귀공식. dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)]로 잘 표현된다. 여기에서 의사신속도 η=-ln[tan(θ/2)]이고, y_b는 실험실계의 입사입자(^32S)의 신속도이다. 이 공식에 의한 적합에서 k=-0.057±0.008로 얻어진다. 즉, 핵건판과 고에너지 중이온의 충돌에서 파쇄 헬륨핵의 exp(η-y_b)의 분포는 "극한파쇄" 현상을 잘 설명하고 있다. The angular distribution of emission angle θ of helium (Z=2) produced in the collisions of incident particles of 200 GeV/nucleon ^32S in nuclear emulsion is well expressed by dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)] where the pseudorapidity is η=-ln[tan(θ/2)], the laboratory system primary rapidity is y_b, and k=-0.057+0.008. The shape of this frequency of occurrence distributions in terms of exp(η-y_b) attests to the validity of the concept of "limiting fragmentation" for helium projectile fragments produced in the projectile fragmentation regions of heavy ion collisions in nuclear emulsion.

Pretreatment HBeAg level and an early decrease in HBeAg level predict virologic response to entecavir treatment for HBeAg‐positive chronic hepatitis B

Kwon, J. H.,Jang, J. W.,Lee, S.,Lee, J.,Chung, K. W.,Lee, Y. S.,Choi, J. Y. Blackwell Publishing Ltd 2012 Journal of viral hepatitis Vol.19 No.2

<P><B>Summary. </B> There are few reports on hepatitis B e antigen (HBeAg) titres during nucleos(t)ide analogues treatment. We investigated the changes in HBeAg levels in patients treated with entecavir and the usefulness of HBeAg quantification for predicting antiviral response. Ninety‐five consecutive HBeAg‐positive patients treated with entecavir for more than 48 weeks were enrolled. Serum levels of hepatitis B surface antigen (HBsAg), HBeAg and HBV DNA were assessed at 4‐week intervals to week 24 and thereafter at 12‐week intervals. Virologic response (Y1VR) was defined as an undetectable HBV DNA level at week 48 of therapy. During 48 weeks, HBeAg and HBV DNA level decreased significantly in a biphasic manner and HBsAg level tended to decease. Fifty‐three patients (55.8%) attained Y1VR. Pretreatment HBeAg levels were significantly lower in the Y1VR group than in no Y1VR group. At week 4 and 12 of therapy, 25% and 41.4% of patients showed a decrease of HBeAg levels with >0.5 log<SUB>10</SUB> and >1.0 log<SUB>10</SUB> from baseline, respectively. These patients achieved more Y1VR than those with less decrease of HBeAg levels (97.7%<I>vs</I> 22.2% and 86.2%<I>vs</I> 29.3%, respectively). HBeAg level at week 12 had higher predictive values for Y1VR than HBV DNA level. Multivariate analysis revealed that a pretreatment HBeAg level of <360 PEIU/mL and the reduction in HBeAg level >1.0 log<SUB>10</SUB> at week 12 were associated with Y1VR. These results suggest that pretreatment HBeAg level and an early decrease in HBeAg level are useful measurements for predicting one‐year virologic response during entecavir treatment.</P>

The antimicrobial peptide human cationic antimicrobial protein-18/cathelicidin LL-37 as a putative growth factor for malignant melanoma

Kim, J.E.;Kim, H.J.;choi, J.M.;Lee, K.H.;Kim, T.Y.;Cho, B.K.;Jung, j.Y.;Chung, K.Y.;Cho, D.;Park, H.J. Sookmyung Women's University Research Institute of 2011 여성과 건강 Vol.6 No.2

Background Recent evidence suggests cathelicidin LL-37 to be a growth factor for various human cancers such as lung cancer, ovarian cancer and breast cancer. However, the effect of LL-37 against malignant skin cancer has not been reported. Objectives To investigate whether the human cathelicidin LL-37 is involved in the carcinogenesis of various skin tumours. Methods Human cationic antimicrobial protein-18 (hCAP-18)/LL-37 production in several cell lines including HaCaT, a chronic myelogenous leukaemia (CML) cell line and various melanoma cell lines was examined using reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay. Immunohistochemical analysis of melanoma, nonmelanoma skin cancer and precancerous and benign skin lesions was performed. After adding LL-37 to a melanoma cell line, tumour cell proliferation, migration and invasion were investigated. Results Human malignant melanoma cell lines overexpressed hCAP-18/LL-37 mRNA and peptide compared with HaCaT and CML cell lines. Immuno- histochemistry showed that the peptide was strongly expressed in malignant melanoma and moderately expressed in squamous cell carcinoma, whereas basal cell carcinoma, precancerous lesions and seborrhoeic keratosis showed no or weak expression. LL-37 also stimulated melanoma cell proliferation, migration and invasion in vitro. Conclusions Cathelicidin LL-37 was primarily expressed in human malignant skin cancer. LL-37 promoted melanoma cell proliferation, migration and invasion in vitro. We report that an increase in the level of LL-37 is associated with malignant skin tumours such as malignant melanoma. These results highlight the importance of LL-37 in the malignant tendency of skin tumours.

각종 갑상선질환에서 혈청 myoglobin치에 관한 연구

정순일,조보연,이문호,장연복,고창순,조경삼,김삼용 대한핵의학회 1980 핵의학 분자영상 Vol.14 No.2

각종 갑상선질환에서 혈청 myoglobin치의 변화를 관찰하고 방사면역측정법으로 혈청 myoglobin치를 측정한 결과 다음과 같은 결과를 얻었다. 1) 치료전 갑상선기능항진증 환자의 혈청 myoglobin 평균치는 39.1±20.3 ng/ml로 정상대조군의 50.9±17.0 ng/ml보다 낮았으나 통계적으로 유의한 차이는 없었다. 2) 갑상선기능저하증 환자의 혈청 myoglobin치는 28∼156 ng/ml로 5예중 3예에서 증가되어 있었다. 3) 혈청 myoglobin치와 혈청 T₃, T₄치와는 유의한 상관관계가 없었다. 4) 갑상선중독성 근병증의 진단과 그 정도 및 치료후 경과관찰의 지표로서 유용할 가치가 있다고 사료된다. To evaluate the change of serum myoglobin in the thyroid disease, we measured serum myoglobin by radioimmunoassay in 24 normal subjects, 34 patients with hyperthyroidism (20 untreated, 14 treated) and 5 patients with hypothyroidism. The mean values of serum myoglobin in 24 normal control, 20 untreated hyperthyroidism and 14 treated hyperthyroidism were 50.9±17.0 ng/ml, 39.1±203 ng/ml and 51.0±18.3 ng/ml respectively. The serum myoglobin level of untreated hyperthyroidism seemed to be lower than that of normal control without statistical significance. The serum myoglobin levels in 5 patients with hypothyroidism ranged 25-156 ng/ml, and 3 patients showed increased serum myoglobin level. We observed one case with severe degree of thyrotoxic myopathy whose myoglobin level was markedly elevated initially and was decreased to normal range after treatment. This results suggests that the determination of serum myoglobin might be useful in the evaluation of thyrotoxic myopathy.

忠南地域 自生春蘭의 分布와 氣象要素

鄭吉雄,李武鉉,金榮七,朴奉圭 公州大學校 資源科學硏究所 1994 資源科學硏究論文集 Vol.2 No.-

忠南地域에 自生하고 있는 春蘭의 各 地域別 分布를 調査하고 氣象環境 중 自生春蘭에 影響을 주는 要因을 分析, 檢討하여 얻은 結果를 要約하면 다음과 같다. 1. 自生春蘭의 分布가 많은 地域은 安眠島를 中心으로 하여 保寧, 泰安, 瑞山, 洪城地域 順이었고, 內陸地域인 靑陽, 禮山地域도 比較的 分布가 많았으며 점차 그 分布가 擴大되고 있는 傾向이었다. 2. 春蘭 自生에 가장 크게 影響을 주는 氣象要素인 1월 중순 最低平均氣溫은 海岸地域인 瑞山과 大川이 各各 -6.8℃, -6.6℃로 內陸地域인 錦山, 儒城의 -8.8℃, -9.6℃보다 높게 나타났고, -8℃以下의 異積日數는 瑞山, 大川이 1∼2일인데 비하여 錦山, 儒城은 30日 以上이 었으며, 春蘭自生의 限界溫度는 -8℃ 程度로 推定된다. 3. 平均 風速은 瑞山이 2.28m/s로 錦山 1.34m/s보다 빠르게 나타났다. 4. 氣象要素중 最低溫度은 平均 및 最高氣溫, 相對濕度, 蒸氣壓, 구름量, 降水量, 蒸發量 등에 正의 相關關係가 있었고, 日較差와는 負의 相關關係를 나타냈다. 5. 自生春蘭의 分布程度가 많은 地域은 保寧, 泰安, 瑞山, 洪城, 舒川 順이었고, 內陸地域인 錦山, 燕崎, 論山地域은 거의 分布하지 않았다. 6. 各 地域別로 自生春蘭의 分布程度는 忠南地域 緯度와의 相關關係는 認定할 수 없었으나, 經度와는 -0.329**(n=172, p<0.01)로 負의 相關關係를 나타내었다 These studies were carried out to investigate the distribution of wild Cymbidium virescens Lindl in Chung-nam, Korea, and to analyse the meteorological elements among locations where have local meteorological observatory. Well-adapted areas of C. virescens were Anmyun island, Poryeong, Taean, Seosan, Hongseong in the west sea area. In the location with mountainous area, adapted areas of C. virescens were Cheongyang, Yesan and its distributions much more increased. Minimum air temperature of mid-January, an important meteorological element of limitation factor on habitat of C. virescens, were higher Seosan and Taechun observed -6.8℃, -6.6℃ respectively than Keumsan and Yuseong observed -8.8℃, -9.6℃repectively. Sum of days below -8℃ were calculated 1 to 2 days at Secosan and Tachun, and were calculated more 30 days at Keumsan and Yuseong. Limited minimum air temperature of mid-January on habitat of C. virescens were predicted -8℃. Average wind speed was more fast Seosan observed 2.28m/s than Keumsan observed 1.34m/s. Minimum air temperature was signification of positive correlation coefficient among average and maximum air temperature, relative humidity, vapour pressure, cloud cover, precipitation, evaporation, but negative correlation coefficient about diurnal change. The locations of densely habitat degree were observed Poryeong, Taean, Seosan Hongseong and Seochun located in the west sea areas. Keumsan, Yeongi and Nonsan located in the mounatainous areas were not or nearly habitat of C. virescens. The latitudinal habitat degree of C. virescens was very signification of correlation coefficient calculated -0.329**(n=172, p < 0.01) but the longitudinal habitat degree was not signification of correlation coefficient.