The Clinicopathological and Prognostic Impact of 14-3-3 Protein Isoforms Expression in Human Cholangiocarcinoma by Immunohistochemistry

Wu, Qiao,Liu, Chang-Zheng,Tao, Lian-Yuan,Yu, Lan,Liu, Wei,Chen, Song-Sen,He, Xiao-Dong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

The 14-3-3 proteins are highly conserved, ubiquitous molecules involved in a variety of biologic phenomena, such as cell cycle control, and apoptosis. However, their expression in cholangiocarcinoma has not been previously characterized. In this paper, immunohistochemistry using specific anti-14-3-3 monoclonal antibodies was performed on formalin-fixed;, paraffin embedded archival tissue from 86 patients of cholangiocarcinoma. We also examined the correlation between expression and survival rate and clinicopathologic factors such as tumor location, tumor size, pathologic differentiation, lymphatic permeation, lymph node metastasis, and tumor stage. Positive 14-3-3 proteins expression was observed for 6 isoforms (${\beta}$, ${\sigma}$, ${\gamma}$, ${\theta}$, ${\delta}$, ${\eta}$) of these proteins in 86 patients of cholangiocarcinoma. ${\beta}$ and ${\sigma}$ isoform immunoreactivity was correlated with lymph node metastasis, tumor stage and patients' survival rate. In addition, ${\delta}$ isoform immunoreactivity showed trends with tumor location, tumor size, pathologic differentiation and tumor stage, while the ${\theta}$ isoform was correlated with pathologic differentiation. These results indicated that upregulated expression of some isoforms of 14-3-3 may be a common mechanism for evading apoptosis in cholangiocarcinoma, so that targeting 14-3-3 may be a novel promising strategy for the treatment of this tumor.

A Novel Effect of Lipids Extracted from Vernix Caseosa on Regulation of Filaggrin Expression in Human Epidermal Keratinocytes

( Wu Qiao ),( Tinghan Jia ),( Hongjian Gu ),( Ruihua Guo ),( Ken Kaku ),( Wenhui Wu ) 대한피부과학회 2019 Annals of Dermatology Vol.31 No.6

Background: Vernix caseosa (VC), which is known as a unique human substance, is a biofilm that covers the skin of most human newborns. VC has many biological functions including anti-infective, skin cleansing and skin barrier repair. Objective: In the study, we purpose to investigate the novel effect of lipids extracted from VC on the regulation of filaggrin (FLG) expression and anti-inflammation in normal human epidermal keratinocyte (NHEK) cells. Methods: The lipids were extracted by chloroform/methanol (Folch method) and the major properties of fatty acid methyl esters were determined with gas chromatography-mass spectrometer. The relative viability of NHEK cells was evaluated by Cell Counting Kit 8 assay. The related expression of skin barrier protein was accessed with real-time quantitative polymerase chain reaction, Western blot and Immunofluorescence in NHEK cells with or without poly (I:C). Meanwhile, the changes of thymic stromal lymphopoietin (TSLP) and tumor necrosis factor alpha (TNF-α) are analyzed by enzyme- linked immunosorbent assay. Results: VC lipids mostly contained saturated and branched chains fatty acids. The expression of mRNA and protein of FLG were significantly increased after the supplement with lipid in NHEK cells. Meanwhile, lipids reversed the inhibition of poly (I:C) on FLG. Moreover, lipids suppressed the over secretion of TSLP and TNF-α induced by poly (I:C). Conclusion: These results indicate that lipids extracted from VC has positive effects on the expression of FLG and anti-inflammation, suggesting that lipids of VC may be used for a reference for novel therapeutic method in reducing and remedying skin disease like atopic disease. (Ann Dermatol 31(6) 611∼620, 2019)

The Metabolic Syndrome and Risk Factors for Biliary Tract Cancer: A Case-control Study in China

Wu, Qiao,He, Xiao-Dong,Yu, Lan,Liu, Wei,Tao, Lian-Yuan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objectives: Recent data show that the metabolic syndrome may play a role in several cancers, but the etiology for biliary tract cancer is incompletely defined. The present aim was to evaluate risk factors for biliary tract cancer in China. Methods: A case-control study in which cases were biliary tract cancer patients referred to Peking Union Medical College Hospital (PUMCH). Controls were randomly selected from an existing database of healthy individuals at the Health Screening Center of PUMCH. Data on the metabolic syndrome, liver diseases, family history, and history of diabetes and hypertension were collected by retrospective review of the patients' records and health examination reports or by interview. Results: A total of 281 patients (102 intrahepatic cholangiocarcinoma (ICC), 86 extrahepatic cholangiocarcinoma (ECC) and 93 gallbladder carcinoma (GC)) and 835 age- and sex-matched controls were enrolled. $HBsAg^+/anti-HBc^+$ (P=0.002), history of diabetes (P=0.000), cholelithiasis (P=0.000), TC (P=0.003), and HDL (P=0.000) were significantly related to ICC. Cholelithiasis (P=0.000), Tri (P=0.001), LDL (P=0.000), diabetes (P=0.000), Apo A (P=0.000) and Apo B (P=0.012) were significantly associated with ECC. Diabetes (P=0.017), cholelithiasis (P=0.000) and Apo A (P=0.000) were strongly inversely correlated with GC. Conclusion: Cholelithiasis, HBV infection and metabolic symptoms may be potential risk factors for the development of biliary tract cancer.

Characteristic profiles of biofilm, enterotoxins and virulence of Staphylococcus aureus isolates from dairy cows in Xinjiang Province, China

Yehui Wu,Jing Li,Mengfan Qiao,Dan Meng,Qingling Meng,Jun Qiao,Xingxing Zhang,Lixia Wang,Kuojun Cai,Jinsheng Zhang,Zaichao Zhang,Weiwei Yu,Xuepeng Cai 대한수의학회 2019 Journal of Veterinary Science Vol.20 No.6

As an important zoonotic pathogen, Staphylococcus aureus (S. aureus) infection has led to serious mastitis and endometritis in infected dairy cows. HereIn this study, a total of 164 strains of S. aureus were isolated from dairy cows in Xinjiang Province, China, and subjected to assays to determine of drug susceptibility and biofilm (BF) formation ability. The eEnterotoxin-related genes were detected, and then the transcription levels of genes related to BF formation were also determined by using reverse transcription-quantitative polymerase chain reactionPCR. Moreover, the pathogenicity of these isolates with different BF formation abilities was determined via by measuring their hemolysis activity, half lethal dose (LD50) and organ bacterial load, respectively. The results showed that 83.4 % of S. aureus isolates could form BF. Among them, 40.8 % of the strains had weak BF formation ability, and most strains with a strong BF formation ability were ica gene carriers. The S. aureus isolates displayed multidrug resistance and their drug resistance was positively correlated with their BF formation ability. Moreover, 96.3 % of the S. aureus isolates carried enterotoxin genes. Among them, the detection rates of the novel enterotoxin genes were higher than thoseat of conventional enterotoxin genes. Furthermore, the isolates with a strong BF formation ability had higher LD50 but lower hemolysis ability and organ bacterial load than thoseat of the isolates with weak or no BF ability. However, those isolates without BF ability caused produced more severe pathological changes than thoseat of the isolates with strong BF formation ability. These findings suggested that higher frequencies of BF ability and presence of novel enterotoxin genes were are important characteristics of S. aureus isolates from dairy cows in Xinjiang Province, China, and such isolateswhich may posed potential threats to food safety.

Evaluation of a Novel Array-Based Toxoplasma, Rubella, Cytomegalovirus, and Herpes Simplex Virus IgG Enzyme Linked Immunosorbent Assay and Its Comparison with Virion/Serion Enzyme Linked Immunosorbent Assays

Dongsheng Wu,Yuanjian Wu,Liuhong Wang,Weidong Xu,Qiao Zhong 대한진단검사의학회 2014 Annals of Laboratory Medicine Vol.34 No.1

Background: The dramatic increase in use of the IgG test for toxoplasma, rubella, cyto- megalovirus (CMV), and herpes simplex virus (HSV) [TORCH] has led to the requirement for a high-efficiency method that can be used in the clinical laboratory. This study aimed to compare the results of BGI-Array ELISA TORCH IgG (BGI-GBI, China) screening method to those of Virion/Serion TORCH IgG ELISA (Virion/Serion, Germany). Methods: Serum specimens (n=400) submitted for routine IgG testing by Virion/Serion ELISA were also tested using the BGI-Array ELISA method. The agreements of these two kinds of method were analyzed by κ-coefficients calculation. Results: Following repeat testing, the BGI-Array ELISA TORCH IgG assays demonstrated agreements of 99.5% (398/400 specimens), 98% (392/400 specimens), 99% (396/400 specimens), and 99.5% (398/400 specimens), respectively. The BGI-Array ELISA IgG as- says provided results comparable to Virion/Serion ELISA results, with κ-coefficients show- ing near-perfect agreement for the HSV (κ=0.87), rubella (κ=0.92) and CMV (κ=0.93) and substantial agreement for the toxoplasma (κ=0.80) IgG assays. The use of the BGI- Array ELISA TORCH IgG assays could reduce the turnaround time (1.5 hr vs. 5 hr by Vi- rion/Serion ELISA for 100 specimens) and were easy to use. Conclusions: BGI-Array ELISA TORCH IgG shows a good agreement with Virion/Serion ELISA methods and is suitable for clinical application.

Macrophages Promote Coal Tar Pitch Extract-induced Tumorigenesis of BEAS-2B Cells and Tumor Metastasis in Nude Mice Mediated by AP-1

Zhang, Peng,Jin, Yue-Fei,Zhang, Qiao,Wu, Yi-Ming,Wu, Wei-Dong,Yao, Wu,Wu, Yong-Jun,Li, Zhi-Tao,Zhao, Yong,Liu, Yu,Feng, Fei-Fei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Background: We sought to evaluate the role of tumor associated macrophages (TAMs) on the promotion of coal tar pitch extract (CTPE)-induced tumorigenesis of human bronchial epithelial cells (BEAS-2B) and tumor metastasis in nude mice, and related mechanisms. Materials and Methods: BEAS-2B cells were first treated with 2.4 mg/mL CTPE for 72 hours. After removal of CTPE, the cells were continuously cultured and passaged using trypsin-EDTA. THP-1 cells were used as macrophage-like cells. BEAS-2B cells under different conditions (n=6/group) were injected into the back necks of nude mice, and alterations of tumor xenograft growth, indicative of tumorigenicity, and tumor metastasis were determined. Pathological changes (tumor nests and microvascular lesions) of HE-stained tumor tissues were also evaluated. The expression of AP-1(c-Jun) in xenografts and metastatic tumors was determined using immunohistochemistry. Results: Tumor size and weight in nude mice transplanted with the mixture of CTPE-induced passage 30 BEAS-2B and THP-1 cells (2:1) were increased compared to those from the CTPE-treated BEAS-2B cells at passage 30 alone at different observation time points. Tumor metastasis to lymph nodes and liver was only detected after transplantation of a mixture the two kinds of cells. The numbers of tumor nests and microvascular lesions, and the expression levels of AP-1 (c-Jun) in tumors from the mixture of two kinds of cells were increased apparently in contrast to those in tumor from the CTPE-treated BEAS-2B cells of passage 30 alone. In addition, there was positive correlation between AP-1 (c-Jun) expression level and the number of microvascular lesions, or between AP-1 (c-Jun) expression level and tumor metastasis in these two groups. Conclusions: TAMs not only facilitate tumorigenesis transformation of CTPE-induced BEAS-2B cells, but also promote tumor growth, angiogenesis and metastasis in nude mice in vivo, which may be mediated by AP-1.

Left Ventricular Remodeling in Patients with Primary Aldosteronism: A Prospective Cardiac Magnetic Resonance Imaging Study

Wu Tao,Ren Yan,Wang Wei,Cheng Wei,Zhou Fangli,He Shuai,Liu Xiumin,Li Lei,Tang Lu,Deng Qiao,Zhou Xiaoyue,Chen Yucheng,Sun Jiayu 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.10

Objective: This study used cardiac magnetic resonance imaging (MRI) to compare the characteristics of left ventricular remodeling in patients with primary aldosteronism (PA) with those of patients with essential hypertension (EH) and healthy controls (HCs). Materials and Methods: This prospective study enrolled 35 patients with PA, in addition to 35 age- and sex-matched patients with EH, and 35 age- and sex-matched HCs, all of whom underwent comprehensive clinical and cardiac MRI examinations. The analysis of variance was used to detect the differences in the characteristics of left ventricular remodeling among the three groups. Univariable and multivariable linear regression analyses were used to determine the relationships between left ventricular remodeling and the physiological variables. Results: The left ventricular end-diastolic volume index (EDVi) (mean ± standard deviation [SD]: 85.1 ± 13.0 mL/m2 for PA, 75.9 ± 14.3 mL/m2 for EH, and 77.3 ± 12.8 mL/m2 for HC; p = 0.010), left ventricular end-systolic volume index (ESVi) (mean ± SD: 35.2 ± 9.8 mL/m2 for PA, 30.7 ± 8.1 mL/m2 for EH, and 29.5 ± 7.0 mL/m2 for HC; p = 0.013), left ventricular mass index (mean ± SD: 65.8 ± 16.5 g/m2 for PA, 56.9 ± 12.1 g/m2 for EH, and 44.1 ± 8.9 g/m2 for HC; p < 0.001), and native T1 (mean ± SD: 1224 ± 39 ms for PA, 1201 ± 47 ms for EH, and 1200 ± 44 ms for HC; p = 0.041) values were higher in the PA group compared to the EH and HC groups. Multivariable linear regression demonstrated that log (plasma aldosteroneto- renin ratio) was independently correlated with EDVi and ESVi. Plasma aldosterone was independently correlated with native T1. Conclusion: Patients with PA showed a greater degree of ventricular hypertrophy and enlargement, as well as myocardial fibrosis, compared to those with EH. Cardiac MRI T1 mapping can detect left ventricular myocardial fibrosis in patients with PA.

Investigation and Simulation Study on the Cascading Trip-off Fault of a Large Number of Wind Turbines in China on May 14, 2012

Qiao, Ying,Lu, Zong-Xiang,Lu, Ji,Ruan, Jia-Yang,Wu, Lin-lin The Korean Institute of Electrical Engineers 2015 Journal of Electrical Engineering & Technology Vol.10 No.6

The integration of the large-scale wind power brings great challenge to the stability of the power grid. This paper investigates and studies the fault on May 14, 2012 of the large-scale cascading trip-off of wind turbines in North China. According to the characteristics of the voltage variation, the fault process is divided into three stages: the pre-event stage, the critical stage before cascading, and the cascading stage. The scenes in the fault are reproduced, using the full-size actual power system model. Simulation models of double-fed induction generators (DFIGs) and SVCs including protection settings and controller strategies are carefully chosen to find out the reason of voltage instability in each stage. Some voltage dynamic that have never been observed before in the faults of the same kind are analyzed in detail, and an equivalent voltage sensitive dynamic model of DFIG is proposed for the fast computation. The conclusions about the voltage dynamics are validated by the actual PMU observation evidence.

Weighted Local Naive Bayes Link Prediction

Wu, JieHua,Zhang, GuoJi,Ren, YaZhou,Zhang, XiaYan,Yang, Qiao Korea Information Processing Society 2017 Journal of information processing systems Vol.13 No.4

Weighted network link prediction is a challenge issue in complex network analysis. Unsupervised methods based on local structure are widely used to handle the predictive task. However, the results are still far from satisfied as major literatures neglect two important points: common neighbors produce different influence on potential links; weighted values associated with links in local structure are also different. In this paper, we adapt an effective link prediction model-local naive Bayes model into a weighted scenario to address this issue. Correspondingly, we propose a weighted local naive Bayes (WLNB) probabilistic link prediction framework. The main contribution here is that a weighted cluster coefficient has been incorporated, allowing our model to inference the weighted contribution in the predicting stage. In addition, WLNB can extensively be applied to several classic similarity metrics. We evaluate WLNB on different kinds of real-world weighted datasets. Experimental results show that our proposed approach performs better (by AUC and Prec) than several alternative methods for link prediction in weighted complex networks.

Erosion-corrosion behaviors of Z2CN19·10N austenitic stainless steel in liquid–solid two-phase flow

Wu Xiangfeng,Li Chengtao,Luo Kunjie,Fang Kewei,Qiao Yanxin 한국물리학회 2021 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.79 No.4

The erosion-corrosion behaviors of Z2CN19·10N austenitic stainless steel in a 3.5-wt% NaCl solution with various sediment contents (7 wt%, 10 wt%, 20 wt%) and flow rates (1 m/s, 3 m/s, 5 m/s) were studied using a rotary disk erosion-corrosion test device, scanning election microscopy (SEM), potentiodynamic polarization test and potentiostatic polarization test. The results showed that sediment concentration had a smaller influence on the electrochemical corrosion behavior of Z2CN19·10N stainless steel than the flow rate and that the flow rate mainly affected the pitting potential and passivation range, but it had little influence on the potential-constant polarization. When the erosion velocity was lower than 5 m/s, mechanical erosion was the main influencing factor, and no critical flow velocities appeared.