Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps Sinensis in the Bronchoalveolar Lavage Fluids Cells

Kuo, Yuh-Chi,Wu, Chia-Lin,Tasi, Wei-Jern,Wang, Jir-Yenn,Chang, Shi-Chung,Lin, Ching-Yuang,Shiao, Ming-Shi 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang₃,Ching-Yung Lin⁴,and Ming-Shi Shiao⁴¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang²,Ching-Yung Lin³,and Ming-Shi Shiao⁴.¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan. Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps sinensis in the Bronchoalveolar Lavage Fluids Cells. Proceedings of international Symposium on East-West Medicine, Seoul. 44-64, 1999.-Cordyceps sinensis is a major parasitic fungus on larva of Lepidoptera. It is one of the well know fungi used in traditional Chinese medicine for treatment asthma, lung and kideney diseases. Many fungi belonging to the genus Cordyceps have been demonstrated to produce natural products with various biological activities. Reported biological activities of genus cordyceps include:(a)inhibition of DNA and RNA synthesis;(b)enhancement of cell differentiation;(c)restruction of cytoskeleton;(d)inhibition of protein kinase activity;(e)antitumor activity on bladder, colon, lung carcinoma as well as fibroblastoma;(f)inhibition of the infection and revers transciptase activity of human immunodeficiency virus type 1; and(g) inhibition of methylation of nucleic acid. Thus, it is suggested that the extracts of Cordyceps sp. Contain antitumor, antiviral, and many other biological activities. In our previous studies, we proved that C. sinensis contains the antitumor and immunomodulatory agents. In the present studies, effects of C. sinensis on bronchoalveolar lavage fluids(BAL)cells were demonstrated. The crude methanolic extracts of C. sinensis were fractionated into 15 fractions by silica gel column chromatography. Effects of various fraction on BAL cells proliferation and lipopolysaccharide(LPS) stimulated interleukin-1β(IL-8),platelet activating factor(PAF), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α),and interleukin-8(IL-8) production on BAL cells were determined. The result indicated that the CS-19-22 fraction suppressed BAL cells proliferation activated by LPS. The median inhibitory concentration(IC50) was 6㎍/ml. The CS-19-22 fraction also decreased IL-1β,PAF,IL-6, TNF-αand IL-8 production.The results of revers transcription-polymerase chain reaction(RT-PCR) demonstrated that the CS-19-22 fraction did not affect IL-1β,PAF,IL-6, TNF-α,and IL-8 mRNAs expression in BAL cells activated by LPS. By contrast, it inhibited the IL-10 mRNA expression but enhanced IFN-γand IL-12 mRNAs expression in activated BAL cells. Moreover, the CS-19-22 fraction blocked PAF-induced platelet aggregation. It is unlikely that cytotoxicity was involved. Because no cell deths were observable. We hypothesize that inhibitory mechanisms of CS-19-22 on BAL cells proliferation may be related to the impairments of gene expression and production of cytokines in BAL cells. The explanation of C. sinensis antiasthma function may involved the following(1)C.sinenesis contained immunomodulatory agents that stimulated IFN-γand IL-12 expression in TH1cells.(2)Enhanced secretion of IFN-γand IL-12 will inhibit the Tγcells immune responses Ex. Decreasing of IL-6 and IL-10 production.(3) The impairments of cytokines production in TH2 cells suppressed th BAL cells proliferation then more cytokines production Ex.IL-1β,PAF,IL-6,TNF-αand IL-8 in activated BAL cells were decreased. (4)The suppression of TH2cells immune responses will inhibit the growth and differentiation of B cells then IgE production will be decreased. (5)Reduced production of IgE will decreased asthma attack occurring in individuals. However, the results of the present study suggested that C. sinensis may also have acted to treat asthma in part by inhibiting BAL cells proliferation and cytokine gene expression and production. Future, experiments with treatment of animals with asthma with C. sinensis will be necessary to defined whether C. sinensis can reduce asthma injury. Plans are underway for the isolation of pure principal growth inhibitors and their mechanisms of action are subjected for further study.

Parametric study of a drivetrain in horizontal wind turbine using torsional model

Wei Shi,Hyunchul Park(박현철),Changwan Kim(김창완),Chinwha Chung(정진화),Youngchan Kim(김영찬) 대한기계학회 2012 대한기계학회 춘추학술대회 Vol.2012 No.5-2

ON A CONJECTURE OF GRAHAM

Wei, Shi Ming,Jun, Young-Bae The Youngnam Mathematical Society Korea 1994 East Asian mathematical journal Vol.10 No.2

Study on the Marine Growth Effect on the Dynamic Response of Offshore wind Turbines

Wei Shi,박현철,백재하,김창완,김영찬,신현경 한국정밀공학회 2012 International Journal of Precision Engineering and Vol.13 No.7

Jacket foundation is regarded as a suitable solution for wind turbines in the intermediate water depths ranging from 30 to 80 m. Numerous types of marine fouling organisms may be found on the submerged members of jacket. Marine growth is found to influence loading of offshore structures by increasing tube diameters, drag coefficient, mass and hydrodynamic added mass and structural weight. In this paper, the types and distribution of marine growth are mentioned. We aimed to investigate the effects of marine growth with different thicknesses, densities and hydrodynamic coefficients values on characteristics of an offshore wind turbine with jacket foundation. The eigen analysis shows that the marine growth has a little effect on the first order natural frequencies while it has higher effect on second and third order natural frequencies of the support structure. Thickness and density have a strong effect on hydrodynamic loads. To obtain the properties of marine growth and hydrodynamic coefficients in the design of offshore wind turbines, full-scale measurements are needed.

DECOMPOSITIONS OF IDEALS IN BCI-ALGEBRAS

Wei, Shi-Ming,Jun, Young-Bae Korean Mathematical Society 1994 대한수학회논문집 Vol.9 No.2

In 1966, Iseki [4] introduced the notion of BCI-algebras which is a generalization of BCK-algebras. The ideal theory plays an important role in studying BCK/BCI-algebras. In this paper we study decompositions of ideals in BCI-algebras, and give a characterization of closed ideals. Also we define ignorable ideals in BCI-algebras, and investigates its properties.(omitted)

WEAKLY POSITIVE IMPLICATIVE BCI-ALGEBRAS

Wei, Shi-Ming,Jun, Young-Bae Korean Mathematical Society 1995 대한수학회논문집 Vol.10 No.4

We introduce the concept of weakly positive implicative ideals in BCI-algebras and give some characterization of weakly positive implicative BCI-algebras and weakly positive implicative ideals.

Dynamic analysis of the wind turbine drivetrain considering shaft bending effect

Wei Shi,박용희,박현철,Dezhi Ning 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.7

In the previous research, shaft torsional flexibility was only considered in the wind turbine drivetrain. However, if shaft is longer and thinner than other parts, two components which are connected by shaft affect each other by rotation about bending axis. It means that there are deflections of shaft about not only torsional direction but also bending direction. In this research, we introduced spherical joint which have 3 spring stiffness about all rotational axis to define shaft. And we analyzed that how shaft bending affect drivetrain rotation, translation motion and gear mesh contact force. To do these processes, we simulated the 3-dimensional wind turbine drive train model which has bearing stiffness, gear mesh stiffness, and shaft flexibility. The gear mesh stiffness was defined by Fourier series. And the equation of motion was acquired by Lagrange equation and kinematical constraints to represent shaft flexibility. About numerical analysis, the Newmark method was used to get results. Lastly, fast Fourier transform which converts results from time domain to frequency was used.

Dynamic Modeling and Analysis of a Wind Turbine Drivetrain Using the Torsional Dynamic Model

Wei Shi,박현철,김창완,정진화 한국정밀공학회 2013 International Journal of Precision Engineering and Vol.14 No.1

A mathematical model for a horizontal axial wind turbine drivetrain was developed using the torsional multibody dynamic model. The drivetrain in this study consisted of a low-speed planetary gear stage (three identical planets with spur teeth, sun and fixed ring gears) and two high-speed spur gear stages. This typical arrangement has been commonly used in the wind turbine industry. The governing equation of the drivetrain was derived by using the Lagrange’s equation, which takes into account the kinetic and potential energies and the work from the input torque. The governing equation was solved numerically by direct numerical integration. Dynamic analysis was carried out to investigate the transient response of the drivetrain system.

ON $Lambda$-ASSOCIATIVE BCI-ALGEBRAS

Wei, Shi-Ming,Jun, Young-Bae Korean Mathematical Society 1995 대한수학회논문집 Vol.10 No.2

In this paper, we introduce the notion of $\lambda$-associative BCI-algebras, investigate important properties of such algebras, and discuss the structure of it.

NON-STATIONARY DYNAMIC ANALYSIS OF A WIND TURBINE DRIVETRAIN

Wei SHI,Ji Kun YOU,De Zhi NING,Zhi Yu JIANG 한국신재생에너지학회 2017 AFORE Vol.2017 No.11