분만 전·후 사료급여 형태가 젖소의 생산성에 미치는 영향

기광석,김현섭,이왕식,이현준,김상범,정하연,은정식,김용국 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

This experiment was carried out to protect drops of feed intake should be plural prepartum and postpartum to reduce metabolic diseases after calving to know how feeding systems, which is divided as a low quility, a high quility and total mixed rations(TMR), affects on postpartum productivity of Holistein cows. Three diets (low or high quality forage separately fed with concentrate and TMR containing high quality roughage) were fed to 21 cows from 3 weeks prepartum to 8 weeks postpartum to examine their effects on the productivity of cows. DM intakes was noticed significantly higher with TMR (17.11kg/day) than low- quality (13.48 kg/day) and high-quality forage (13.10kg/day). TDN and CP intakes were also higher with TMR compared to other experimental diets. Mean daily milk yield was non-significant among the cows fed different diets. Blood non-esterified fatty acids(NEFA) content was higher in cows fed low-quality or high-quality forage separately with concentrate compared with those fed TMR. The results concluded that TMR feeding to transitional cows is better than feeding the low or high quality forage separately for their health and productivity. 본 시험은 분만 전 3주부터 분만 후 3주까지 전환기라고 지칭되는 시기의 젖소들에 대한 대사적 변화를 최소화하기 위한 방법으로 사료 섭취량 저하를 방지하고 분만 후 대사성 질병의 발생을 줄이기 위한 목적으로 수행되어졌다. 대사성 질병에 관여하는 주요 요인으로는 건물섭취량, 혈중 glucose 함량, NEFA 함량, 반추위 환경, 면역 등인데, 분만 전·후 Holstein 젖소에 사료급여 형태를 조·농분리(저질 조사료와 양질 조사료 급여구) 및 TMR로 다르게 하였을 때 사료섭취량 변화와 혈중 NEFA 및 glucose 함량 변화를 측정하고 분만 후 젖소의 생산성에 미치는 영향을 구명하기 위하여 수행하였다. 처리별 사료건물 섭취량은 저질 조사료 조·농 분리, 양질조사료 조·농 분리, TMR 급여구에서 각각 13.48, 13.10, 17.11kg으로 TMR 급여구에서 유의성 있게 높았으며(P〈0.05), TDN 섭취량은 처리구별 각각 7.35, 7.19, 5.66kg으로 처리간에 차이가 없었다(P〉0.05). 신체충실지수(BCS)는 저질조사료구에서는 3.03이였으며, 양질 조사료구와 TMR 급여구에서는 각각 3.19, 3.16이었다. 분만후 1주부터 5주까지 측정한 평균 산유량은 저질조사료 조·농 분리, 양질 조사료 조·농 분리, TMR 급여구에서 각각 34.1, 35.1, 35.6kg 였으며, 분만 후 4∼5주에 최고 비유기에 달했다. 처리별 평균 유지율은 저질 조사료 조·농 분리, 양질 조사료 조·농 분리, TMR 급여구에서 각각 3.74, 3.68, 3.95%였다. 혈청내 NEFA 함량은 저질 조사료 조·농 분리, 양질 조사료 조·농 분리, TMR 급여구에서 각각 744.4, 381.8,266.7μEq/L로 처리간에 큰 차이를 보여 양질 조사료 급여와 TMR 급여시 혈중 NEFA 함량이 저질 조사료 급여에 이해 유의성 있게 낮았다(P〈0.05). 혈청내 glucose함량은 저질조사료 및 양질 조사료 조·농 분리, TMR 급여구에서 각각 39.3, 56.3, 62.4mg/dl로 양질 조사료 급여와 TMR 급여시 혈중 glucose 함량이 저질 조사료급여에 비해 유의성 있게 높았다(P〈0.05). 이상의 결과들을 종합해 볼 때, 분만 전 3주부터 분만 후 3주까지의 전환기 동안 사료섭취량 증가와 대사성 질병의 지표로서 혈중 NEFA 및glucose 함량 변화를 살펴 본 결과 분만 전 3주부터는 양질의 조사료나 TMR 형태로 급여하는 것이 사료섭취량 증가와 대사성 질병의 발생을 감소시킬 수 있을 것으로 사료되었다.

효모 세포벽 분해효소 생산균의 탐색 및 효소생산 최적조건의 조사

차성관,최혜숙,김왕준,윤석후,김영배 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

담자효모 세포벽을 분해하는 효소 생산균의 탐색을 위하여 50여 토양시료와 방선균 1000여개의 미생물을 가지고 3차에 걸친 탐색작업을 실시한 결과 1차적으로 autolysed washed yeast 배지 위에서 투명한 생성크기로 493개 균주를 선발하였으며, 이 균주들을 2차적으로 생효모 한천배지 위에서의 투명한 생성 크기로 117개 균주를 선발 하였다. 선발된 균주들을 가지고 용해활성도의 측정방법을 이용하여 최종적으로 St-1702 균주가 선발되었고, St-1702 균주는 동정 실험결과 잠정적으로 Streptomyces eurythermus 또는 그 유연균으로 동정되어졌다. St-1702 균주를 이용하여 효소생산 최적조건을 조사한 결과 최적배지 및 배양조건은 다음과 같았다 : freeze dried yeast 2% glucose 1%, K_2HPO_4 1%, MgSO_4·7H_2O, 1% peptone 0.5% (NH_4)_2CO_3 0.2% 초기 pH 값 7.0의 배지 및 30℃에서 3일간 배양. 최적배지에서 선발균주를 배양한 배양액을 이용하여 담자효모들의 원형질체 형성 실험을 한 결과 시중에서 구입된 효모 세포벽 분해 효소들보다 더 높은 원형질체 형성률을 보여 주었다. Thousand actinomycetes and 50 soil samples were used for the isolation of microorganisms producing yeast cell wall lytic enzymes. Among 493 strains producing large clear zones on autolysed washed yeast (AWY), 117 strains were selected on living yeast cell agar plates. With the method of lytic activity, one strain (St-1702) was selected, which was temporarily identified as Streptomyces eurythermus. The optimal condition for enzyme production of this strain was partially determined as follows: incubation of the strain for 3 days at 30℃ in the medium containing 2% freeze dried yeast cell, 1% glucose, 1% K_2HPO_4, 0.01% MgSO_4·^7H_2O, 0.5% peptone, and 0.2% (NH_4)_2CO_3 with pH 7.0. The protoplast formation of yeast by using the enzyme produced by this strain was compared with commercial enzymes.

Selection of Human-Originated Lactobacillus acidophilus For Production of Probiotics

KIM, WANG-JUNE,HONG, SEOK-SAN,CHA, SEONG-KWAN 한국미생물 · 생명공학회 1994 Journal of microbiology and biotechnology Vol.4 No.2

Lactobacillus acidophilus KFRI 233, a strain isolated from human, was selected as a candidate for probiotics due to its excellent growth in MRS broth where no special anaerobic condition is required. Both simultaneous and deferred agar diffusion assays exhibited Lb. acidophilus KFRI 233 to possess an antagonistic effect against Clostridium perfringens. Its antagonistic effect was pH dependent Associative culture of KFRI 233 and Cl. perfringens in broth resulted in maximum 94.04% inhibition of Cl perfringens. β-Galactosidase activity of KFRI 233 was higher than other tested strains that are sold as commercial probiotics. Survival of KFRI 233 in pasteurized skim milk (4℃) and Sherbet mix (-15℃) after 7 days of storage were 71.9 and 105.5%, respectively.

Screening of Bacteriocinogenic Lactic Acid Bacteria and Antagonistic Effects in Sausage Fermentation

( Wang June Kim ) 한국미생물생명공학회 1996 Journal of microbiology and biotechnology Vol.6 No.6

A Brief Analysis on the Regional Appearance in the Subway Entrances and Ventilation Pavilion Design in the new period -The Thinking about Urban Rail Traffic Entrances and Ventilation Pavilion Design in Zhengzhou-

Wang Lipei,Kim June Bong 국제온돌학회 2013 International Journal of Ondol Vol.6 No.1

In the design of urban rail traffic entrances and ventilation pavilion in the city of Zhengzhou, Henan province, this study tries to explore a kind of regional appearance about the subway entrances and ventilation pavilion design in the new period through the combination of sustainable building ideology [ 1] and local culture. The creation inspiration of the design is from the aspects of humanism, and the design tries to make the building have distinct regional characteristic in the premise of the contemporary feeling with the rational method. The building is located in the central plains , where the cultural resources are very rich. The design tries to choose clements that are from the famous cultural heritage and appeared appropriately in the plan, which to read and promote culture with the perspective of modem style architecture, and also make the subway entrance building have local differences and the identify ability. In the deepening of the design, the green and sustainable thought is the main exploration direction. The design tries to use High-tech building materials, and shows the building texture with the Combination of ETFE (ethylene-tetra-fluoric-ethylene) and steel skeleton, which appear the structure and the form of the building in the new period with the eco-friendly style. At the same time, the design discusses the way of Subway Entrances and the ventilation pavilion in central plains under the constraints of geography, climate and function.

Inhibitory Effects of Acetic Acid and Temperature on Growth of Campylobacter jejuni ATCC 33291

(Wang June Kim),(Soon Young Shin),(Han Joon Hwang) 한국미생물생명공학회 2001 Journal of microbiology and biotechnology Vol.11 No.6

N/A N/A

Bacteriocins of Lactic Acid Bacteria: Their Potentials as Food Biopreservative

Kim,Wang June 이화여자대학교 가정과학대학 식품영양학과 아시아 식품영양연구소 1996 食品營養情報 Vol.- No.7

Numberous strains of lactic acid bacteria used in the fermentation of foods are known to produce bacteriocins. In general, bacteriocins are a group of proteinaceous antimicrobial substances that inhibit the growth of closely related bacteria. However, some bacteriocins of lactic acid bacteria exhibit relatively broad range of antimicrobial spectrum among which several food spoilage and health threatening microorganisms are included. Many researches have been exerted on the use of bacteriocins as food preservative to extend shelf-life of various foods. The following review will describe researches that have been conducted on bacteriocinogenic lactic acid bacteria(LAB), principally isolated from a wide variety of foods and partially from animal origin, and characteristics of bacteriocins. Special emphasis were put on their potentials as food preservative, physicochemical nature, antibacterial spectrum, application to foods, and genetics.

Plasmid Linkage of Bacteriocin Production and Sucrose Fermentation Phenotypes in Pediococcus acidilactici M

KIM, WANG-JUNE,HA, DUK-MO,RAY, BIBEK 한국미생물 · 생명공학회 1991 Journal of microbiology and biotechnology Vol.1 No.3

Pediococcus acidilactici strain M produced a bacteriocin which was proteinaceous, heat stable, and exhibited antimicrobial activity against lactic acid bacteria, variety of food spoilage and pathogenic bacteria. The antimicrobial activity was not caused by H_2O_2 and organic acid, and was remained between pHs of 4.0 to 9. Molecular weight of crude bacteriocin was approximately 2,500. Phenotypic assignment after plasmid cruing experiment demonstrated that a 53.7 kilobase (kb) plasmid, designated as pSUC53, was responsible for the sucrose fermentation phenotype (Suc^+) and a 11. 1 kb plasmid, designated as pBAC11, was associated with bacteriocin production phenotype (Bac^+). Neither of the two plasmids were linked to antibiotic resistance.

Use of Bacteriocinogenic Pediococcus acidilactici in Sausage Fermentation

Kim, Wang June,Hong, Seok San,Cha, Seong Kwan,Koo, Young Jo 한국미생물 · 생명공학회 1993 Journal of microbiology and biotechnology Vol.3 No.3

The bacteriocin produced by Pediococcus acidilactici KFRI 168 exhibited a wide antimicrobial spectrum including many strains of lactic acid bacteria, Listeria monocytogenes, Staphylococcus aureus, and Enterococcus faecium by both disk and deferred assay methods. Inhibition of Lis. monocytogenes and Stph. aureus were observed only from deferred assay. Gram-negative bacteria were not inhibited. Bacteriocin production was observed at 10 h, and was maximized at 16 h in MRS broth incubated at 37℃. In a beaker sausage fermented with P. acidilactici KFRI 168, viable counts of Stph. aureus, Salmonella, Escherichia coli, Clostridium perfringens, and Lis. monocytogenes were reduced by 2.8, 2.3, 2.4, 0.7, and 0.5 log CFU/g, respectively. Inoculated P. acidilactici KFRI 168 maintained its viable count of more than 10^8 CFU/g during the whole fermentation period, and it took less than 8 h to reduce sausage pH below 5.

Characteristics of Bacteriocin and Mucin Production Phenotypes in Lactobacillus plantarum 27

Kim, Wang June,Ha, Duk Mo,Bibek Ray 한국미생물 · 생명공학회 1991 Journal of microbiology and biotechnology Vol.1 No.2

Phenotypic changes after plasmid curing experiment suggested that the bacteriocin production phenotype (Bac^+) might be linked to a chromosomal DNA and the mucin production phenotype (Muc^+) might be linked to a 62.5 kilobase (kb) plasmid (pMUC62) in Lactobacillus plantarum 27 isolated from meat starter culture. The non-mucoid (Muc^-) variants were missing pMUC62 but they produced bacterlocin as the wild strain (Bac^+). There was no difference in antibiotic resistance and sugar fermentation patterns between the wild strain (Bac^+ Muc^+) and the nonmucoid (Bac^+ Muc^-) variants. Antimicrobial spectrum of bacteriocin produced by both wild strain and Muc^- variant of Lb. plantarum 27 included strains of Pediococcus acidilactici (A, M, H), Pediococcus sp. isolated from meat, Lactobacillus sp. isolated from meat, Lb. plantarum NCDO 955 and Staphylococcus aureus 485. Neither of the tested Gram negative bacteria were inhibited by bacteriocin. Antimicrobial activity of crude bacteriocin was retained after autoclaving, DNase or catalase treatment and exposure from pHs 4 to 9 but was lost after treating with several proteolytic enzymes and exposure at pH 10.