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RISS 인기검색어
- 검색키워드
- 저자 : ( Ti Ling Chang ) (검색결과 2 건)
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Molecular Characterization of tgd057, a Novel Gene from Toxoplasma gondii
Wan, Kiew-Lian,Chang, Ti-Ling,Ajioka, James W. Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.4
The expressed sequence tag (EST) effort in Toxoplasma gondii has generated a substantial amount of gene information. To exploit this valuable resource, we chose to study tgd057, a novel gene identified by a large number of ESTs that otherwise show no significant match to known sequences in the database. Northern analysis showed that tgd057 is transcribed in this tachyzoite. The complete cDNA sequence of tgd057 is 1169 bp in length. Sequence analysis revealed that tgd057 possibly adopts two polyadenylation sites, utilizes the fourth in-frame ATG for translation initiation, and codes for a secretory protein. The longest open reading frame for the tgd057 gene was cloned and expressed as a recombinant protein (rd57) in Escherichia coli. Western analysis revealed that serum against rd57 recognized a molecule of ~21 kDa in the tachyzoite protein extract. This suggests that the tgd057 gene is expressed in vivo in the parasite.
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Molecular Characterization of tgd057, a Novel Gene from Toxoplasma gondii
( Kiew Lian Wan ),( Ti Ling Chang ),( James W. Ajioka ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.4
The expressed sequence tag (EST) effort in Toxoplasma gondii has generated a substantial amount of gene information. To exploit this valuable resource, we chose to study tgdO57, a novel gene identified by a large number of ESTs that otherwise show no significant match to known sequences in the database. Northern analysis showed that tgdO57 is transcribed in this tachyzoite. The complete cDNA sequence of tgdO57 is 1169 bp in length. Sequence analysis revealed that tgdO57 possibly adopts two polyadenylation sites, utilizes the fourth in-frame ATG for translation initiation, and codes for a secretory protein. The longest open reading frame for the tgdO57 gene was cloned and expressed as a recombinant protein (rd57) in Escherichia coli. Western analysis revealed that serum against rd57 recognized a molecule of -21 kDa in the tachyzoite protein extract. This suggests that the tgdO57 gene is expressed in vivo in the parasite.
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