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      • KCI등재

        En Bloc Spondylectomy for Spinal Metastases: Detailed Oncological Outcomes at a Minimum of 2 Years after Surgery

        Masayuki Ohashi,Toru Hirano,Kei Watanabe,Kazuhiro Hasegawa,Takui Ito,Keiichi Katsumi,Hirokazu Shoji,Tatsuki Mizouchi,Ikuko Takahashi,Takao Homma,Naoto Endo 대한척추외과학회 2019 Asian Spine Journal Vol.13 No.2

        Study Design: Retrospective case series. Purpose: To investigate the oncological outcomes, including distant relapse, after en bloc spondylectomy (EBS) for spinal metastases in patients with a minimum of 2-year follow-up. Overview of Literature: Although EBS has been reported to be locally curative and extend survival in select patients with spinal metastases, detailed reports regarding the control of distant relapse after EBS are lacking. Methods: We conducted a retrospective review of 18 consecutive patients (median age at EBS, 62 years; range, 40–77 years) who underwent EBS for spinal metastases between 1991 and 2015. The primary cancer sites included the kidney (n=7), thyroid (n=4), liver (n=3), and other locations (n=4). Survival rates were estimated using the Kaplan–Meier method, and groups were compared using the log-rank method. Results: The median operative time and intraoperative blood loss were 767.5 minutes and 2,375 g, respectively. Twelve patients (66.7%) experienced perioperative complications. Five patients (27.8%) experienced local recurrence of the tumor at a median of 12.5 months after EBS, four of which had a positive resection margin status. Thirteen patients (72.2%) experienced distant relapse at a median of 21 months after EBS. The estimated median survival period after distant relapse was 20 months (95% confidence interval, 0.71–39.29 months). No association was found between resection margin status and distant relapse. Overall, the 2-year, 5-year, and 10-year survival rates after EBS were 72.2%, 48.8%, and 27.1%, respectively. Importantly, the era in which EBS was performed did not impact the oncological outcomes. Conclusions: Our results suggest that EBS by itself, even if margin-free, cannot prevent further dissemination, which occurred in >70% of patients at a median of 21 months after EBS. These results should be considered and conveyed to patients for clinical decision-making.

      • Vocabulary Analyzer Based on CEFR-J Wordlist for Self-Reflection (VACSR) Version 2

        Yukiko Ohashi,Noriaki Katagiri,Takao Oshikiri Institute for Corpus Research 2023 Asia pacific journal of corpus research Vol.4 No.2

        This paper presents a revised version of the vocabulary analyzer for self-reflection (VACSR), called VACSR v.2.0. The initial version of the VACSR automatically analyzes the occurrences and the level of vocabulary items in the transcribed texts, indicating the frequency, the unused vocabulary items, and those not belonging to either scale. However, it overlooked words with multiple parts of speech due to their identical headword representations. It also needed to provide more explanatory result tables from different corpora. VACSR v.2.0 overcomes the limitations of its predecessor. First, unlike VACSR v.1, VACSR v.2.0 distinguishes words that are different parts of speech by syntactic parsing using Stanza, an open-source Python library. It enables the categorization of the same lexical items with multiple parts of speech. Second, VACSR v.2.0 overcomes the limited clarity of VACSR v.1 by providing precise result output tables. The updated software compares the occurrence of vocabulary items included in classroom corpora for each level of the Common European Framework of Reference-Japan (CEFR-J) wordlist. A pilot study utilizing VACSR v.2.0 showed that, after converting two English classes taught by a preservice English teacher into corpora, the headwords used mostly corresponded to CEFR-J level A1. In practice, VACSR v.2.0 will promote users' reflection on their vocabulary usage and can be applied to teacher training.

      • KCI등재후보

        STM TIP-CURRENT-INDUCED POLYMERIZATION OF C60, Ce2@C80 AND Lu2@C76

        HISANORI SHINOHARA,KAZUNORI OHASHI,NOBUYUKI FUKUI,MASAHIRO AKACHI,TAKAO AKACHI,HISASHI UMEMOTO,YASUHIRO ITO,TOSHIKI SUGAI,RYO KITAURA 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2009 NANO Vol.4 No.5

        Polymerization of C60, Ce2@C80, and Lu2@C76 metallofullerenes induced by electron injection from a scanning tunneling microscope (STM) tip has been studied. C60 polymerization has been observed only with C60 multilayer on Au(111) and not with C60 monolayer on Au(111). The Ce2@C80 metallofullerene on Si(111)-7 × 7 also polymerizes by electron injection but has less polymerization reactivity than C60. Interestingly, the Lu2@C76 metallofullerene on Si(111)-7 × 7 does not show any polymerization. Based on these STM observations on the tip-induced polymerization, the direction of the bond formation and the dependence of reactivity on the kind of fullerenes are discussed.

      • SCIESCOPUSKCI등재

        Biosynthetic Pathway of Indole-3-Acetic Acid in Basidiomycetous Yeast Rhodosporidiobolus fluvialis

        ( Sakaoduoen Bunsangiam ),( Varunya Sakpuntoon ),( Nantana Srisuk ),( Takao Ohashi ),( Kazuhito Fujiyama ),( Savitree Limtong ) 한국균학회 2019 Mycobiology Vol.47 No.3

        IAA biosynthetic pathways in a basidiomycetous yeast, Rhodosporidiobolus fluvialis DMKUCP293, were investigated. The yeast strain showed tryptophan (Trp)-dependent IAA biosynthesis when grown in tryptophan supplemented mineral salt medium. Gas chromatography-mass spectrometry was used to further identify the pathway intermediates of Trpdependent IAA biosynthesis. The results indicated that the main intermediates produced by R. fluvialis DMKU-CP293 were tryptamine (TAM), indole-3-acetic acid (IAA), and tryptophol (TOL), whereas indole-3-pyruvic acid (IPA) was not found. However, supplementation of IPA to the culture medium resulted in IAA peak detection by high-performance liquid chromatography analysis of the culture supernatant. Key enzymes of three IAA biosynthetic routes, i.e., IPA, IAM and TAM were investigated to clarify the IAA biosynthetic pathways of R. fluvialis DMKU-CP293. Results indicated that the activities of tryptophan aminotransferase, tryptophan 2-monooxygenase, and tryptophan decarboxylase were observed in cell crude extract. Overall results suggested that IAA biosynthetic in this yeast strain mainly occurred via the IPA route. Nevertheless, IAM and TAM pathway might be involved in R. fluvialis DMKU-CP293.

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