Development of a noninvasive KIM-1-based live-imaging technique in the context of a drug-induced kidney-injury mouse model

Tae-Jun Kwon,Da-Sol Lee,Md. Enamul Haque,Rang-Woon Park,Byungheon Lee,Dongkyu Kim,Yong-Hyun Jeon,Kil-Soo Kim,Sang Kyoon Kim 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

The development of reliable methods to diagnose acute kidney injury is essential to allow the adoption of early therapeutic interventions and evaluate their effectiveness. Based on the fact that kidney injury molecule-1 (KIM-1) expression levels in kidneys are markedly upregulated early after a damage event, here we developed a noninvasive KIM- 1-based molecular imaging technique to detect kidney injury. First, we took advantage of a phage-display platform to select small peptides demonstrating a specific high binding affinity to KIM-1. The promising candidate was conjugated with fluorescent probes, and its imaging potential was validated in vitro and in vivo. This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of KIM-1 expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model. Our data overall suggest that the CNRRRA peptide is a promising probe to use in the context of in vivo imaging for the detection of KIM-1 overexpression in damaged kidneys.

장기간 항갑상선제 투여 후 임의 중단 뒤 재복용시 발생한 무과립구증 1 예

김승만;김태영;박수영;배영환;서영경 정은주;김태균;권민정;박정현;이순희 인제대학교 2011 仁濟醫學 Vol.32 No.-

Agranulocytosis, one of the major side effects of antithyroid a gents, probably occurs in less than 0.25% of cases treated with antithyroid agents. It usually occurs within the first several weeks or months after the initiation of therapy, when drug dosage is higher. Most reported cases of agranulocytosis have occurred during the first 3 months of therapy. We describe here a rare case that did not experience the side effect for 6 years of antithyroid agent treatment but presented with agranulocytosis in one month after re-exposure. It suggests that the major side effect such as agranulocytosis can occur in patients who had undergone prior uneventful antithyroid drug therapy.

Clinical Outcome of Modified Cervical Lateral Mass Screw Fixation Technique

Kim, Seong-Hwan,Seo, Won-Deog,Kim, Ki-Hong,Yeo, Hyung-Tae,Choi, Gi-Hwan,Kim, Dae-Hyun The Korean Neurosurgical Society 2012 Journal of Korean neurosurgical society Vol.52 No.2

Objective : The purpose of this study was 1) to analyze clinically-executed cervical lateral mass screw fixation by the Kim's technique as suggested in the previous morphometric and cadaveric study and 2) to examine various complications and bicortical purchase that are important for b-one fusion. Methods : A retrospective study was done on the charts, operative records, radiographs, and clinical follow up of thirty-nine patients. One hundred and seventy-eight lateral mass screws were analyzed. The spinal nerve injury, violation of the facet joint, vertebral artery injury, and the bicortical purchases were examined at each lateral mass. Results : All thirty-nine patients received instrumentations with poly axial screws and rod systems, in which one hundred and seventy-eight screws in total. No vertebral artery injury or nerve root injury were observed. Sixteen facet joint violations were observed (9.0%). Bicortical purchases were achieved on one hundred and fifty-six (87.6%). Bone fusion was achieved in all patients. Conclusion : The advantages of the Kim's technique are that it is performed by using given anatomical structures and that the complication rate is as low as those of other known techniques. The Kim's technique can be performed easily and safely without fluoroscopic assistance for the treatment of many cervical diseases.

Melt spinning 법으로 제조된 Ti-Ni-Cu 합금리본의 상변태거동 및 형상기억특성

金載勳,崔美善,李喜佑,金泰俊,김연욱,南泰鉉 대한금속재료학회 2002 대한금속·재료학회지 Vol.40 No.7

Phase transformation behavior and shape memory characteristics of Ti-Ni-Cu alloy ribbons fabricated by melt spinning have been investigated by means of differential scanning calorimetry, X-ray diffraction, optical microscopy and thermal cycling tests under constant load. With increasing linear velocity and decreasing ejection pressure, transformation start temperatures of ribbons decreased and stability of the B19 martensite was lowered, comparing to those of alloys fabricated by conventional casting since the cooling rate increased. Only after heat treating ribbons at temperatures higher than 873 K where recrystallization occurred, the B19 martensite was observed in Ti-40Ni-10Cu alloy ribbons. Transformation elongation and hysteresis associated with the B2-B19 transformation in Ti-Ni-Cu alloy ribbons decreased with increasing Cu-content, which was attributed to the decrease in lattice deformations. Transformation elongations of ribbons were larger than those of alloys made by casting, which was closely related to a texture developed in ribbons.

멀티 플랫폼상의 컴포넌트 연동 방법 연구

김정현,김태웅,김태공,전순미,최항묵 인제대학교 2002 仁濟論叢 Vol.17 No.1

소프트웨어 개발 방법론이 객체지향개발 방법론(OOD : Object Oriented Development)에서 점차 컴포넌트 기반 개발 방법론(CBD : Component Based Development)으로 변화되어 가는 추세이다. 경험이 많은 개발자에 의해 개발된 컴포넌트는 소프트웨어 재사용이 뛰어나고, 이미 많은 곳에서 사용 중이므로 안정성 및 신뢰성이 인정된다. 하지만 개발언어와 플랫폼의 특성에 따라 컴포넌트의 조합에 있어 어려움이 따른다. 이에 본 연구에서는 HTTP와 XML을 이용하여 개발언어와 플랫폼에 독립적으로 컴포넌트를 조합할 수 있는 방안에 대해 연구하고 기존의 컴포넌트에 HTTP와 XML을 이용한 컴포넌트 호출을 보인다.

슬롯후드 입구 균일류 형성 조건

김환태,김태형,하현철,김은아 경성대학교 환경문제연구소 2000 環境硏報 Vol.10 No.1

Slot hoods are most commonly used to provide uniform exhaust air flow and an adequate capture velocity over finite length of contaminant generation, e.g. an open surface tank or over the face of a large hood such as a side-draft design. The function of the slot is solely to provide uniform air distribution. Industrial Ventilation Manual edited by ACGIH (American Conference of Governmental Industrial Hygienists) recommended the conditions to obtain uniform air distribution . slot velocity higher than 10m/s and take-off angle larger than 45 degree. But, in reality, it might not be an easy task to satisfy the conditions for uniform air distribution. By using CFD (Computational Fluid Dynamics), the 81 cases (3 aspect ratios x 3 mean slot velocities x 3 take-off angles x 3 duct velocities) were thus simulated and the coefficients of variance were computed to compare the uniformity of slot face velocities. Generally speaking, either aspect ratio or duct velocity do not affect the uniformity a lot while both slot velocity and take-off angle play a crucial role in forming uniform air distribution.

유제놀에 의한 즉시형 알레르기 반응의 억제

김상현,신태용,김형룡,이영미,이은희,신보경,김윤철,안년형,김형민 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

The current study evaluates the capacity of eugenol to regulate immediate allergic reaction by control of histamine release. Administrations of eugenol (1 M/kg. i.p.) at 60 min before and 5.10 min after the compound 48/80 treatment (8 mg/kg.i.p.) were shown the mortality rates as 0.44.4. and 77.8%. respectively. A 60 min before administered group revealed a singificant inhibition of serum his-tamine release compared with those of 5 and 10 min after the compound 48/80 injection. Eugenol (6-48mM) was also showed a dose-dependent activity on the compound 48/80-induced histamine release from the highly purified population of Alcian Blue-positive peritoneal mast cells. These results indicate that in vitro treatment with exogenous eugenol inhibited the active response of mast cell populations and modulated its characteristics.

경희대학교 평화의 전당 노래비 디자인

김동찬,김호겸,민창기,김태현,김용욱 경희대학교 디자인연구원 2003 예술· 디자인학연구 Vol.6 No.1

The purpose of this study is to design a basic draft of a monumental inscription with a poem of a peace for stirring up a peaceful mind. The inscription is expected to be a place for arousing a peaceful mind to visitors. Moreover, this place can be a place for culture and rest, which uplifts the image and phase of Kyunghee University to both.

비혈연간 동종골수이식에 의해 치료된 중증 재생불량성빈혈 1예

김현중,이홍기,이방훈,이창근,이종태,정현식,김원석,윤성수,강원기,박근칠,김대식,고영혜,박찬형 대한조혈모세포이식학회 1997 대한조혈모세포이식학회지 Vol.2 No.1

비혈연간 동종골수이식은 혈연간 동종골수이식이 불가능한 환자에서 기존의 면역역제요법으로 효과가 없었던 경우나 재발한 경우에 적응증이 될수 있다. 이식 거부와 이식편대숙주병의 발생이 골수 이식의 성공률을 감소시키므로, 보다 강력한 conditioning regimen의 사용과 T cell이 제거된 골수를 사용하는 것이 필요하다. 기존의 high dose corticosteroid나 cyclosporine에 대하여 불응성인 이식편대숙주병의 치료를 위해서 ATG와 Mycophenolate mofetil (Cellcept®) 의 사용이나 Tacrolimus, Methotrexate와 같은 다른 면역억제제치료를 고려해 볼 수 있다. It is known that allogenic bone marrow transplantation is the most effective treatment for aplastic anemia. However, this treatment is only applicable to a minority of patients because the proportion of patients who have HLA-matched siblings about 25% to 30% of all the aplastic anemia patients. In the case of the absence of HLA-matched donor, unrelated allogenic bone marrow transplantation is considered. However, (because of severe acute and chronic GVHD and graft failure) the success rate of this approach is only 20~30%. Recently, it was reported that increased immunosuppressive condition combined with T cell depletion of the marrow graft would result in greater success. There was no case of successfully treated aplastic anemia patient by unrelated allogenic bone marrow transplantation in Korea. Here, we report a case of severe aplastic anemia successfully treated with unrelated allogenic bone marrow transplantation.

Breeding of Tetraploid in Platycodon grandiflorum (Jacq.)A. DC. by Colchicine treatment

Kim,Ik-Hwan,Kim,Hag-Hyun,Hong,Eui-Yon,Yun,Jong-Sun,Yun,Tae,Hwang,Ju-Kwang,Lee,Cheol-Hee 한국자원식물학회 2003 Plant Resources Vol.6 No.3

Present studies were carried out to produce tetraploid plants by colchicine treatment using seeds, seedlings and shoot tips of Platycodon grandiflorum in Campanulaceae. The most successful colchicine treatment for tetraploid production in P. grandiflorum was soaking treatment using 0.01 and 0.5% colchicine solution for 1 hour and 12 hours, respectively. Morphological characteristics of both diploid and tetraploid were similar, but tetraploid plants had more leaves. Compared to diploid, tetraploid had the larger stomata, but less number of stomata. Fresh weight of tetraploids was 20∼40% heavier than that of diploid.