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강용구,이태원,이종찬,최환 金烏工科大學校 産業技術開發硏究院 1999 産業技術開發硏究 Vol.15 No.-
A diamond core drill has been well carried out in the ceramics drilling. In this investigation, a vertical type machining center (MC) was used in the coring of Al₂O₃and Si₃N₄ceramics with a metal bonded diamond core drill. To clarify the mechanism of coring, the influences of coring conditions on the coring force, grinding ratio, succesive zone of coring were studied. At the wheel feed speed of 5.2 mm/min, and the wheel revolution of 3000rpm in Al₂O₃ceramics, the wheel speed of 2.7 mm/min, and the wheel revolution of 1500rpm in Si₃N₄ceramics, the best grinding ratio was obtained. But, from a viewpoint of coring speed, the wheel feed speed at 37 mm/min (Al₂O₃) and 10 mm/min (Si₃N₄) were best, when the coring depth was to 1 mm.
탄소강재의 회전굽힘응력 하에서의 피로균열의 성장식과 수명예측
강용구,최환,이태원,이종찬 國立金烏工科大學校附設生産技術硏究所 1997 産業技術開發硏究 Vol.13 No.-
In this work, fatigue crack growth equations and Life Predition are studied. New exponential crack growth equation are presented to describe the crack growth behaviors from an artificial pit of three type carbon steels (G. J1 and J2) under rotary bending (R= -1). Crack growth curves obtained from new exponential crack growth equations are in good agreement with experimental data of carbon steels. Fatigue life predition are also carried out by numerical integral method. Predition lives using new exponential crack growth equations and 4th order polynominal crack growth equations are also compared with experimental results. Predition lives obtained from new exponential crack growth equation are in good agreement with the experimental ones. The difference between predition lives and experimental ones are not exceed ±10%. The other hand, in the case of using 4th order polynominal crack growth equations, the difference between predition lives and exprimental ones are exceed ±20%.
선인장 양심실 보조장치의 설계 및 임상적용을 위한 평가
민병구,박찬영,최재순,이혁수,황창모,김삼성,윤걸중,김종원,선경,이경갑,정종태,김원곤 제주대학교 인공심장이식연구소 2001 인공심장 연구 Vol.2 No.1
현재 개발되고 있는 맥동형 이식형 인공심장은 완전이식형 인공심장과 좌심실보조장치 뿐이다. 좌심실보조장치를 장착한 환자의 10~15%가 우심실의 보조를 필요로하고 완전이식형 인공심장의 장착을 위해서는 자연심장을 제거해야하는 상황에서 이식형 양심실보조장치에 대한 필요성이 대두되고 있다. 본 연구진은 완전이식형 인공심장으로 개발된 한국형 인공심장을 개선하여 양심 실 보조장치를 개발하였다. 양심실보조장치는 이동형 작동기식 미케니즘을 이용하고 있으며 에너지 변환장치와 감속기로 구성된 작동기와 혈액주머니, 그리고 내장형 제어기로 구성되어 있다. 선인장 펌프로 명명된 KAH350은 캐뉼라의 연결을 위한 커넥터를 포함하여 길이 177mm, 폭 164mm, 높이 67 이고 무게 780g이며 최대 심박출량은 5L/min 이다. 양심실보조장치로 개발된 선인장펌프는 좌심실보조장치로도 응용이 가능하다. 이식적합성과 생체적합성을 평가하기 위하여 좌심실보조장치로 5회, 양싱실보조장치로 6회의 동물실험을 수행하였다. 각각 최장 28일간 생존하였으며 11회의 동물실험중 장치의 결함에 의한 것이 3회 있었는데 이들은 모두 전자장치의 결함과 방수처리문제였다. 현재 이식적합성과 일박출량을 향상시킨 KAH400모델이 개발중이다. The types of pulsatile implantable artificial hearts that are on the way of development and have been developed are totally implantable artificial heart and implantable left ventricular assist device (LVAD). Approximately 10% to 15% of all patients Implanted with wearable VADs have required right heart support with another device. And it is reluctant to patient who should remove his or her own heart to be implanted with total artificial heart. These situations drive the development of implantable bi-ventricular assist device (BVAD). The Korean BVAD was develophed by modifying the moving actuator type Korean artificial heart. This electro-mechanical BVAD comprises actuator including energy converter and reduction gear train, blood sacs, and internal motor and energy controllers. The KAH350 which is named as 'Cactus Pump' is 177mm in length, 164mm in width, and 67mm in thickness including connectors and nuts. The weight and maximum cardiac output of Cactus Pump is 780g and 5L/min, respectively. The Cactus Pump that was developed as BVAD could be used as LVAD by attaching compliance caps on the 2 ports of one ventricle. The animal experiments were undergone 5 times for LVAD, and 6 times for BVAD. The best records were 28 days survival in both applications. There were 3 times of device failure and they were all associated with electrical connection and hermetic sealing. The KAH400 that has improved anatomical fitting characterisitcs and stroke volume is on development.
A Stong Ubiquitous Activity of Bombyx mori Heat Shock Protein 70 Promoter
Tae-Won Goo,Sung-Wan Kim,Yong-Bo Kim,Seong-Ryul Kim,Seung-Won Park,Seok-Woo Kang,Kwang Gill-Lee,O-Yu Kwon,Eun-Young Yun 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05
For stable germline transformation, the promoter of B. mori cytoplasmic actin gene (BmA3) was used to ubiquitous expression of transgenes. Except for BmA3 promoter, promoters used to regulate gene expressionin all tissues and developmental stages of B. mori were not nearly developed. To identify more powerful promoter than previously reported BmA3 promoter (Mange et al., 1997), we introduced a new dot blot hybridization method, and isolated nine clones that show stronger dot signal compared to the control, BmA3by this method. Among these 9 clones, we focused on one clone which has high amino acid homology (94%) with heat shock protein 70 gene of Trichoplusia ni. This resulting positive clone, named bHsp70 (B. mori heat shock protein 70) was ubiquitiously expressed in tissues and developmental stage of fifth instar B. mori larvae,and stimulated bythermal and ER stress. As result of promoter assay using dual luciferase assay system, we found the highest transcription activity region (-1003/+147) in the 5'-flanking region of bHsp70 gene that has 264-fold more intensive promoter activity than BmA3 promoter. Moreover, transcription activity of bHsp70 promoter under heat shock condition (42 ℃, 4 hr) was increased over 2-fold than normal condition. Therefore, we suggest that bHsp70 promoter may be used more effective candidate for transgene expression in B. mori.
Utilization of the Bombyx mori Hypothetical Protein 32 Promoter for Efficient Transgene Expression
Goo, Tae-Won,Kim, Sung-Wan,Kim, Seong-Ryul,Park, Seung-Won,Kang, Seok-Woo,Lee, Kwang-Gill,Kwon, O-Yu,Yun, Eun-Young Korean Society of Sericultural Science 2010 International Journal of Industrial Entomology Vol.20 No.2
For stable germline transformation, the promoter of Bombyx mori cytoplasmic actin gene (BmA3) has been used for ubiquitous expression of transgenes. So far, no strong promoter is available for ubiquitous expression in B. mori, excluding BmA3 promoter. To identify more powerful promoter than previously reported BmA3 promoter, we isolated 9 clones that show stronger signal compared to BmA3 by a dot blot hybridization. Among these 9 clones, we focused on one clone which has high amino acid homology (85%) with hypothetical protein 32 gene of Lonomia obliqua. This clone, named bHp32 (B. mori hypothetical protein 32) was ubiquitously expressed in all tissues and developmental stage of fifth instar B. mori larvae. As result of promoter assay using dual luciferase assay system, we found the highest transcription activity region (-1,200/+220) in the 5'-flanking region of bHp32 gene, which has 42-fold more intensive promoter activity than BmA3 promoter. Moreover, the bHp32 promoter was normally regulated in Bm5, Sf9, and S2 cells. Therefore, we suggest that bHp32 promoter may be used more powerful and effectively for transgene expression in various insects containing B. mori as a universal promoter.
Goo, Tae-Won,Yun, Eun-Young,Kim, Sung-Wan,Park, Kwang-Ho,Hwang, Jae-Sam,Kwon, O-Yu,Kang, Seok-Woo Korean Society of Sericultural Science 2003 International Journal of Industrial Entomology Vol.7 No.2
Protein disulfide isomerase (PDI) found in the endoplasmic reticulum (ER) catalyzes disulfide bond exchange and assists in protein folding of newly synthesized proteins. PDI also functions as a molecular chaperone and has been found to be associated with proteins in the ER. In addition, PDI functions as a subunit of two more complex enzyme systems: the prolyl-4-hydroxylase and the triacylglycerol transfer proteins. A cDNA that encodes protein disulfide isomerase was previously isolated from Bombyx mori (bPDI), in which open reading frame of 494 amino acids contained two PDI-typical thioredoxin active site of WCGHCK and an ER retention signal of the KDEL motif at its C-terminal, and we report its functional characterization here. This putative bPDI cDNA is expressed in insect Sf9 cells as a recombinant proteins using baculovirus expression vector system. The bPDI recombinant proteins are successfully recognized by antirat PDI antibody, and shown to be biologically active in vitro by mediating the oxidative refolding of reduced and scrambled RNase. This suggests that bPDI may play an important role in protein folding mechanism of insects.
A Bombyx mori Transcription Factor, ATFC Binds Directly to the UPRE of Molecular Chaperones
Goo, Tae-Won,Yun, Eun-Young,Kim, Sung-Wan,Park, Kwang-Ho,Hwang, Jae-Sam,Kwon, O-Yu,Kang, Seok-Woo Korean Society of Sericultural Science 2003 International Journal of Industrial Entomology Vol.7 No.2
Cells respond to an accumulation of unfolded proteins in the endoplasmic reticulum (ER) by increasing transcription of genes encoding molecular chaperones and folding enzymes. The information is transmitted from the ER lumen to the nucleus by intracellular signaling pathway, called the unfolded protein response (UPR). In Saccharomyces cerevisiae, such induction is mediated by the cis-acting unfolded response element (UPRE) which has been thought to be recognized by Hac1p transcription factor. We cloned the ATFC gene showing similarity with Hac1p, and then examined to determine whether ATFC gene product specifically binds to UPRE by electrophoretic mobility shift assays. ATFC gene product displayed appreciable binding ${to ^{32}}P-labelled$ UPRE. Therefore, we concluded that ATFC represents a major component of the putative transcription factor responsible for the UPR leading to the induction of ER-localized stress proteins.