성인에서 횡격막 탈장에 동반하여 발생한 급성 췌장염의 증례

오수윤 ( Suyun Oh ),백창렬 ( Chang-nyol Paik ) 대한췌담도학회 2018 대한췌담도학회지 Vol.23 No.4

현재까지 보고된 문헌에 따르면 횡격막 탈장에 동반되어 발생한 급성 췌장염의 사례는 드문 것으로 알려져 있다. 횡격막 탈장 시 급성 췌장염이 발생하는 기전은 췌장 자체의 탈장, 탈장으로 인해 췌장의 견인이나 위의 급격한 팽창으로 인해 발생하는 췌장의 허혈로 설명된다. 이 증례는 명치 통증과 호흡곤란을 주소로 내원한 78세 남자환자에서 영상의학적 검사 및 혈액학적 검사 등을 통하여 췌장의 탈장 없이, 횡격막 탈장에 동반되어 발생한 급성 췌장염을 확인한 증례이다. 환자는 고령임을 감안하여 수술적 치료 없이 보존적 치료만을 시행하였고, 보존적 치료만으로 상태가 호전이 되었으며 34개월 후에도 증상의 재발이 없음을 확인하였다. 현재까지 횡격막 탈장에 의해서 발생한 급성 췌장염에서 정립된 표준 치료는 없으나, 증례를 살펴보면 대부분 위식도 열공 탈장의 수술적 교정을 시행한 후 췌장염이 호전되었고, 수술의 고위험군 환자의 경우 보존적 치료만으로 성공적으로 치료된 경우가 소수 보고되었다. 이 증례를 통하여 저자는 위식도 열공 탈장이 있는 환자에서 급성 췌장염을 유발할 만한 일반적인 원인이 없음에도 불구하고 급성 췌장염이 발생한 경우 횡격막 탈장에 동반한 췌장염의 가능성을 고려해야 함을 설명하고자 하였다. Acute pancreatitis with diaphragmatic hernia is rare, and furthermore, very few were reported in the case without incarceration. The suggested mechanism of acute pancreatitis with diaphragmatic hernia is that herniation of pancreas itself. The other possible mechanism is pancreatic ischemia due to traction after aggravation of hernia or acute distention of stomach. We report a case of acute pancreatitis due to diaphragmatic hernia with no evidence of herniation of pancreas. A 78-year-old male was administered for epigastric pain and dyspnea. The radiologic and laboratory result demonstrated an acute pancreatitis with diaphragmatic hernia without any evidence of herniation of pancreas. The patient was managed conservatively to reduce the hernia and to treat pancreatitis. Korean J Pancreas Biliary Tract 2018;23(4):177-181

Effect of Mixing and Placing in Hot Weather on Hardened Concrete Properties

Suyun Ham,Taekeun Oh 한국콘크리트학회 2013 International Journal of Concrete Structures and M Vol.7 No.2

Portland cement concrete exposed to high temperatures during mixing, transporting, casting, finishing, and curing can develop undesirable characteristics. Applicable requirements for such the hot weather concrete differ from country to country and government agencies. The current study is an attempt at evaluating the hardened properties of the concrete exposed to hot weather in fresh state. First of all, this study reviews the current state of understanding and practice for hot weather concrete placement in US and then roadway sites with suspected hot weather concrete problems were investigated. Core samples were obtained from the field locations and were analyzed by standard resonance frequency analysis and the boil test. Based on the results, there does not appear to be systematic evidence of frequent cracking problems related to high temperature placement. Thus, the suspicious deteriorations which are referable to hot weather concreting would be due to other factors.

Characterization of diverse pepper (Capsicum spp.) germplasm based on agro-morphological traits and phytochemical contents

Suyun Moon,Ho-cheol Ko,Nayoung Ro,SuKyeung Lee,Hyeonseok Oh,Bichsaem Kim,Bichsaem Kim,Gi-An Lee 한국육종학회 2023 한국육종학회 공동학술발표집 Vol.2023 No.-

Construction of a CRISPR/Cas9-Mediated Genome Editing System in Lentinula edodes

( Suyun Moon ),( Jee Young An ),( Yeon-jae Choi ),( Youn-lee Oh ),( Hyeon-su Ro ),( Hojin Ryu ) 한국균학회 2021 Mycobiology Vol.49 No.6

CRISPR/Cas9 genome editing systems have been established in a broad range of eukaryotic species. Herein, we report the first method for genetic engineering in pyogo (shiitake) mushrooms (Lentinula edodes) using CRISPR/Cas9. For in vivo expression of guide RNAs (gRNAs) targeting the mating-type gene HD1 (LeA1), we identified an endogenous LeU6 promoter in the L. edodes genome. We constructed a plasmid containing the LeU6 and glyceraldehyde-3-phosphate dehydrogenase (LeGPD) promoters to express the Cas9 protein. Among the eight gRNAs we tested, three successfully disrupted the LeA1 locus. Although the CRISPRCas9-induced alleles did not affect mating with compatible monokaryotic strains, disruption of the transcription levels of the downstream genes of LeHD1 and LeHD2 was detected. Based on this result, we present the first report of a simple and powerful genetic manipulation tool using the CRISPR/Cas9 toolbox for the scientifically and industrially important edible mushroom, L. edodes.

Construction of a CRISPR/Cas9-Mediated Genome Editing System in Lentinula edodes

Moon, Suyun,An, Jee Young,Choi, Yeon-Jae,Oh, Youn-Lee,Ro, Hyeon-Su,Ryu, Hojin The Korean Society of Mycology 2021 韓國菌學會誌 Vol.49 No.6

CRISPR/Cas9 genome editing systems have been established in a broad range of eukaryotic species. Herein, we report the first method for genetic engineering in pyogo (shiitake) mushrooms (Lentinula edodes) using CRISPR/Cas9. For in vivo expression of guide RNAs (gRNAs) targeting the mating-type gene HD1 (LeA1), we identified an endogenous LeU6 promoter in the L. edodes genome. We constructed a plasmid containing the LeU6 and glyceraldehyde-3-phosphate dehydrogenase (LeGPD) promoters to express the Cas9 protein. Among the eight gRNAs we tested, three successfully disrupted the LeA1 locus. Although the CRISPR-Cas9-induced alleles did not affect mating with compatible monokaryotic strains, disruption of the transcription levels of the downstream genes of LeHD1 and LeHD2 was detected. Based on this result, we present the first report of a simple and powerful genetic manipulation tool using the CRISPR/Cas9 toolbox for the scientifically and industrially important edible mushroom, L. edodes.

결식 우려 아동을 위한 위치 기반 아동급식카드 가맹점 안내 서비스

홍건우(Gunwoo Hong),김수윤(Suyun Kim),김수홍(Suhong Kim),박수현(Suhyeon Park),최동수(Dongsoo Choi),오병우(Byoungwoo Oh) 한국정보기술학회 2023 Proceedings of KIIT Conference Vol.2023 No.6

S-515 Posterior reversible encephalopathy syndrome in hypertensive patient undergoing chronic hemodialysis

( Bong Gyu Kwak ),( Kyun Young Kim ),( Suyun Oh ),( Yu Ah Hong ),( Hyeon Seok Hwang ),( Yoon Kyung Chang ),( Suk Young Kim ) 대한내과학회 2016 대한내과학회 추계학술발표논문집 Vol.2016 No.1

Posterior reversible encephalopathy syndrome (PRES) is clinical and radiologic syndrome that accompanied by a headache, altered mental state, seizures and other neurological signs with radiologically by reversible changes on brain image. Although the pathophysiology of PRES is incompletely understood, renal failure was known as one of the risk factors. Regarding end-stage renal disease (ESRD) and PRES, only a few cases of adults on hemodialysis have been described in the literature. We report an interesting case of PRES with severe hypertension receiving chronic hemodialysis. A 55-year-old male with ESRD was admitted to our hospital due to traumatic hemothorax and performed video-assisted thoracoscopic surgery with chest tube insertion. During 3 weeks after operation, his systolic blood pressure (BP) was very huge fluctuation between 80 mmHg to 200 mmHg. Despite using anti-hypertensive medication and hemodialysis, it is very difficult to control BP and pulmonary congestion. Suddenly he was observed decreased mentality and had a seizure-like movement with pulseless electrical activity. He was returned to spontaneous rhythm after successful cardiopulmonary resuscitation for 4 minutes with comatose mentality. We found symmetrical white matter edema in posterior cerebrum in T2-wighted Brain MRI and administrated anticonvulsant and performed continuous renal replacement therapy (CRRT) for 23 days to maintain systolic BP between 100 mmHg to 120 mmHg. His neurologic symptoms had disappeared completely after we controlled strict blood pressure. Hemodialysis patients presenting with severe hypertension and subsequent seizures and unconsciousness should undergo brain MRI to rule out the possibility of PRES and consider to maintain appropriate BP and volume control using CRRT.

S-490 sarcoidosis presenting as pancreatitis, duodenal ulcer and severe acute kidney injury

( Kyun Young Kim ),( Bong Gyu Kwak ),( Suyun Oh ),( Won Seok Park ),( Yoon Kyung Chang ),( Suk Young Kim ),( Hyeon Seok Hwang ),( Yu Ah Hong ) 대한내과학회 2016 대한내과학회 추계학술발표논문집 Vol.2016 No.1

Sarcoidosis is a multisystemic inflammatory disease characterized by the presence of noncaseating granulomas, mainly the lung and lymph nodes. We report an interesting case of sarcoidosis simultaneously involving the gastrointestinal tract, pancreas, and kidney and presenting as severe acute kidney injury (AKI). A 41-year-old man was admitted to our hospital with anorexia and weight loss over several months. Four months ago, he was diagnosed with idiopathic acute pancreatitis and recovered after steroid treatment. At that time, abdominal computed tomography (CT) showed diffuse pancreas swelling without solid mass. We started hemodialysis due to severe AKI with hypercalcemia and renal biopsy was performed. The histopathology indicated granulomatous interstitial nephritis with diffuse interstitial inflammation and noncaseating granulomas. Esophagogastroduodenoscopy revealed ulcerative and edematous mucosa on the stomach and the second part of the duodenum. Chest CT showed a slight interstitial pattern but definite hilar lymphadenopathy was not observed. Noncaseating granulomas were observed on microscopic examination in stomach, duodenum and bronchus. There was no involvement of sarcoidosis in any other organs. He received pulse methylprednisolone followed by maintenance prednisone therapy. After 2 months of treatment, renal function and hypercalcemia were improved. We suggest that renal sarcoidosis can manifest without typical pulmonary involvement and unusual extrathoracic organ involvement occurs with renal sarcoidosis presenting as severe AKI. Active enforcement of biopsy for suspicious lesions including kidney would provide the diagnostic clue in sarcoidosis with unusual manifestation and it supports the histological evidence of granulomas consistent with sarcoidosis. Keywords: sarcoidosis; kidney; pancreas; duodenum