녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

의과대학생 및 전공의들의 영양교육의 필요성에 대한 인식

장현숙,권정숙 대한영양의학회 1999 영양의학 Vol.1 No.3

유방암의 위와 대장전이

유현아,김은영,서민지,정은,조민정,오현진,장지혜,박지찬,이정의,박석영 이화여자대학교 의과학연구소 2014 EMJ (Ewha medical journal) Vol.37 No.2

Gastric metastasis from breast cancer is rare and only six cases have been reported in Korea. Colon metastasis is more rare than gastric metastasis. We report a 63-year-old woman with gastric and colon metastases of invasive lobular carcinoma of breast. She was diagnosed as right breast cancer, received right modified radical mastectomy 10 years ago and has been treated with chemotherapy and hormone therapy. Investigating for melena and a small caliber of stool, we found gastric and colon metastases. The diagnosis of metastatic breast cancer was made through gross pathologic and immunohistochemistry staining. We report a case with gastric and colon metastases from breast cancer and a review of the associated six case reports in Korea.

흰쥐 생체시료 중 5-플루오로우라실 및 테가푸르의 안정성

장지현,박종국,강진형,정석재,심창구,구효정 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.3

5-Fluorouracil (5-FU) is an antimetabolite anticancer agent active against many types of solid tumors. Tegafur (TF), a prodrug of 5-FU, is frequently used in combination with uracil as dihydropyrimidine dehydrogenase (DPD) inhibitory fluoropyrimidine. We studied the stability of 5-FU and TF in biological fluids of rats and determined their bioavailability (BA) and excretion into bile, and urine. The drug concentrations were analyzed by an HPLC method. At room temperature, there was a 14-30% decrease in the concentration of 5-FU and TF in bile, urine, and plasma specimen at 10 and 100 μg/ml over 240 min. No significant difference was noted among the sample types or between two different concentrations of 10 and 100 μg/ml. The decrease in drug concentration was significantly less in samples kept on ice (6-12%) for both drugs. These data indicate that biological fluid samples containing 5-FU or TF in plasma, urine, or bile should be placed on ice during the sample collection. Following these storage guidelines, samples were collected after administration 50 ㎎/㎏ of each drug via i.v. or oral route. BA was 1.5 folds greater for TF (60%) than that of 5-FU (42%). Approximately 0.52 and 3.3% of the i.v. doses of 5-FU and TF was excreted into bile, respectively. Renal clearance of 5-FU was about 16% of its total body clearance. These results suggest that instability of 5-FU and TF in biological fluids should be considered in pharmacokinetic or pharmacogenomic studies.

Plyometric Training과 비타민 C 투여가 남고생의 체력, 혈중 젖산, 암모니아 및 젖산탈수소효소에 미치는 영향

장준원,김세종,이현미,서혜림,조현숙,고정림,염종우,예정복,백영호 釜山大學校 附設 體育科學硏究所 2003 體育科學硏究所 論文集 Vol.19 No.-

The purpose of this was to examine the effect of plyometric training and vitamin C ingestion on physical fitness and fatigue substance....

운동선수들의 상태불안과 특성불안 수준에 관한 연구

장창현,김석일,김성욱 한국스포츠리서치 2000 한국 스포츠 리서치 Vol.11 No.3

The primary purpose of this investigation was to determine the difference of state anxiety level on exercise abilities. exercise careers and trait anxiety level of university male athletes. Individual(n=14) and team (n=124) university male athletes constituted sample of the study, their age range being 17 to 23 years with a mean age of 20.15 yr. 138 university male athletes were divided into elite athlete group and non-elite athlete group according to exercise abilities. On the other hand athletes were divided into high group. middle group and low group according to exercise careers and trait anxiety level. State-trait anxiety completed the State-Trait Anxiety Inventory(STAI) on two occasions at common practice(7 day prior to actual game) and before game(1 course prior to actual game). The STAI was chosen as the major indicator of anxiety. It can be admin istered repeatedly to the same group of subjects. contained separate scales for STAI-S and trait anxiety (STAI-T). and has been used in other studies of exercise and anxiety. Analysis of the result showed that the elite athlete group. high exercise career group and low trait anxiety group and lower state anxiety scores than the non-elite athlete group, low exercise career group and high trait anxiety group on two occasions measures.

결과의 지식(K.R) 제시유형이 농구기능 학습에 미치는 효과

장인석,신현주,진성룡,김종환,김천년 慶熙大學校 體育科學大學 1990 體育科學論叢 Vol.- No.3

The purpose of this study is to investigate the effect of K.R on motor learning of basketball. The subject of this experiment were 90 women students, the second class highschool. They were no apparent physical defects and experiment of basket ball. The subject were divided into three groups ; verbal feedback, visual feedback and verbal+visual feedback groups. In each groups, 30 students were assigned. For the study, the skill test were pass, dribble, shot and combination of basketball. Each group was taught for 12 hours of basketball skill selected for the study. However, teaching methods were different a way of furnished K.R. The classes were three of basketball progam of physical education curriculum at high school. For the data analysis, one-way analysis varianace, turky's-test was employeed. As a result of this study, conclusions were as follow : 1. In part of both pass skill and shot skill, the verval+visual feedback groups were most effcetive among the other groups. 2. For the dribble skill, the verval+visual feedback group was more effective than the verval feedback group. 3. In the case of combination-skill, there have been no significant difference among the three groups.

석면함유 슬레이트 지붕 물받이 퇴적물 중 석면 섬유 함유율

임지현,한솔민,김현석,신유민,박시은,허정윤,김민영,장봉기 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

This study attempted to determine the degree of asbestos release from the aging slate roof by comparing the asbestos content in the slate roof rain gutter with a colored steel plate (tin plate) over the slate roof. Four slate roof houses located in Haengmok-ri, Asan-si, Chungcheongnam-do, and one house constructed with a colored steel plate on the slate roof were selected to collect the sediment of the roof rain gutter. The asbestos fiber content was calculated by a point counting method using a polarization microscope after pretreatment with conversion treatment and hydrochloric acid treatment. The average asbestos content of the four slate roof rain gutter were 1.89%. However, asbestos was not detected in the Slate covering roof rain gutter, which were constructed on the slate roof. Asbestos fiber content was the highest at 2.89% in the slate roof rain gutter installed in 1976, followed by 2.44% in 1953. From the above results, it is necessary to minimize secondary damage as asbestos fibers released from slate roof houses to the surrounding atmosphere or leaked from slate roofs as rainwater may cause soil pollution and seriously affect residents' health. Although covering with colored steel plates (tin plates) has been shown to prevent the leakage of asbestos fibers to some extent, it is believed that a policy alternative to remove the slate roof as soon as possible is needed to solve the fundamental problem.

N-치환 발린 유도체의 합성

姜錫久,洪狀厚,趙顯星 성균관대학교 기초과학연구소 1986 論文集 Vol.37 No.2

As synthetic studies directed towards fluvalinate insecticide, N-substituted valine derivatives were synthesized; S-(+)-valine ethylester was reacted with 1-chloro-2-nitro-4-triflurormethylbenzene to give N-(2-nitro-4-trifluoromethylphenyl) valine ethylester in a nucleophilic aromatic substitution reaction with conservation in optical activity. Also S-(+)-valine ethylester was reacted with 1-chloro-2,4-dinitro benzene to afford N-(2,4-dinitrophenyl) valine ethylester.

인라인스케이트 선수들의 진로의사결정 유형과 관련변인에 관한 연구

김장환,박진기,최현숙 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.3

The purpose of this study is to examine various types and factors influencing decision-makings for athletic careers through analysis by academic backgrounds, athletic achievements, influence of other people, and self-conception. The results of this study are as follows. First, while there were no significant differences in types and degrees of maturity, high school students pursuing college entrance indicated higher scores than college students whose majors were already decided in the degrees of maturity. Individuals and groups with higher athletic achievements had significant differences in adaptation to athletic life and team satisfaction rather than those with lower achievements. Second, there were significant differences in adaptation to athletic life, and as its sub-factors, team satisfaction and inter-relationship with colleagues, coaches, and managers by influence of other people. As positive evaluations were made by important people and self-conceptions were higher, reasonable decision-making were done in adaptation to athletic life. On the contrary, as negative evaluations were made by important people and self-conceptions were lower, intuitive and dependant types indicated significant differences in team satisfaction and inter-relationship with colleagues, coaches, and managers. The more positive influences they had, the more reasonable types they chose. Third, moral, physical, academic and social self-conceptions of athletes indicated significant differences in adaptation to athletic life, team satisfaction, and inter-relationship with colleagues, coaches, and managers.