Priming of Autoreactive CD8<SUP>+</SUP> T Cells Is Inhibited by Immunogenic Peptides Which Are Competitive for Major Histocompatibility Complex Class I Binding

Sooseong You,Yoon Seok Choi,Seokchan Hong,Eui-Cheol Shin 대한면역학회 2013 Immune Network Vol.13 No.3

Priming of Autoreactive $CD8^+T$ Cells Is Inhibited by Immunogenic Peptides Which Are Competitive for Major Histocompatibility Complex Class I Binding

You, Sooseong,Choi, Yoon Seok,Hong, Seokchan,Shin, Eui-Cheol The Korean Association of Immunobiologists 2013 Immune Network Vol.13 No.3

In the present study, we investigated if priming of autoreactive $CD8^+T$ cells would be inhibited by competitive peptides for major histocompatibility complex (MHC) class I binding. We used a mouse model of vitiligo which is induced by immunization of $K^b$-binding tyrosinase-related protein 2 (TRP2)-180 peptide. Competitive peptides for $K^b$ binding inhibited IFN-${\gamma}$production and proliferation of TRP2-180-specific $CD8^+T$ cells upon ex vivo peptide restimulation, while other MHC class I-binding peptides did not. In mice, the capability of inhibition was influenced by T-cell immunogenicity of the competitive peptides. The competitive peptide with a high T-cell immunogenicity efficiently inhibited priming of TRP2-180-specific $CD8^+T$ cells in vivo, whereas the competitive peptide with a low T-cell immunogenicity did not. Taken together, the inhibition of priming of autoreactive $CD8^+T$ cells depends on not only competition of peptides for MHC class I binding but also competitive peptide-specific $CD8^+T$ cells, suggesting that clonal expansion of autoreactive T cells would be affected by expansion of competitive peptide-specific T cells. This result provides new insights into the development of competitive peptides-based therapy for the treatment of autoimmune diseases.

Korean Studies on Blood Stasis: An Overview

Park, Bongki,You, Sooseong,Jung, Jeeyoun,Lee, Ju Ah,Yun, Kyung-Jin,Lee, Myeong Soo Hindawi Publishing Corporation 2015 Evidence-based Complementary and Alternative Medic Vol.2015 No.-

<P>Blood stasis is one of the important pathological concepts in Korean medicine. We analyzed the Korean studies concerning blood stasis. We searched for articles in eight electronic databases from their inception to September, 2014. We included reviews, clinical studies, and preclinical studies that had studied blood stasis and excluded articles in which blood stasis was not mentioned or in which the original authors had not explained blood stasis. Of 211 total included studies, 19 were reviews, 52 were clinical studies, and 140 were preclinical articles. “Stagnant blood within the body” was the most frequently mentioned phrase of the traditional concept of blood stasis. Traumatic injury was the most frequently studied disease/condition in the clinical studies. In the preclinical studies, coagulopathy was studied most frequently, followed by hyperviscosity, hyperlipidemia, inflammation, neoplasm, ischemic brain injury, and atherosclerosis. Hyeolbuchukeo-tang and Angelicae Gigantis Radix were the most frequent formula and single herb, respectively, used in the blood stasis researches. The results showed that blood stasis was mainly recognized as disorder of circulation and many studies showed the effectiveness of activating blood circulating herbs for diseases and pathologies such as traumatic injury or coagulopathy. Further studies are needed in the pathologic mechanisms and various diseases of blood stasis.</P>

Restoration of miR-223-3p expression in aged mouse uteri with Samul-tang administration

Kim Jihyun,You Sooseong 한국한의학연구원 2022 Integrative Medicine Research Vol.11 No.2

Background: During the secretory phase of the estrous cycle, endometrium senescence is accompanied by biological mechanisms such as metabolic dysfunction and epigenetic changes, leading to decreased embryo receptivity and implantation failure. Samul-tang has been reported to improve implantation potential in aged mice. Methods: To uncover the age-related changes in the transcriptomes, we performed QuantSeq 3mRNA sequencing to compare the mRNA expression patterns in the uteri between young and old mice. Young and old female BALB/c mice were administered distilled water or Samul-tang for 4 weeks, and the corresponding effects on the uteri of aged mice were investigated. Results: We found 586 differentially expressed genes between the young and old mouse groups. Functional annotation analysis revealed 10 important pathways, including arachidonic acid metabolism and glutathione metabolism, involved in uterine cellular proliferation and decidualization. Using in silico analysis, we identified the three most abundantly interacting microRNAs–miR-223–3p, 155–5p, and 129–5p– with differentially expressed genes associated with important biological pathways. Samul-tang administration restored the expression of miR-223–3p, which could interact with important genes such as histamine ammonia-lyase (Hal) and acid phosphatase 5 (Acp5) for embryo implantation. Conclusion: Our study demonstrated that uterine aging changes the expression of genes involved in metabolic pathways that are crucial to uterine health, leading to cellular senescence. We postulate that the regulation of miR-223–3p via Samul-tang administration can be of therapeutic importance in cellular senescence.

A systematic review of herbal medicines for the treatment of cancer cachexia in animal models

Park, Bongki,You, Sooseong,Cho, William C. S.,Choi, Jun-Yong,Lee, Myeong Soo Zhejiang University Press 2019 Journal of Zhejiang University. Science B. Biomedi Vol.20 No.1

Programmed death-1 (PD-1)-dependent functional impairment of CD4(+) T cells in recurrent genital papilloma.

Chang, Dong-Yeop,Song, Sang Hoon,You, Sooseong,Lee, Jino,Kim, Jihye,Racanelli, Vito,Son, Hwancheol,Shin, Eui-Cheol Springer-Verlag Italia 2014 Clinical and experimental medicine Vol.14 No.3

<P>Genital papilloma is caused by human papilloma virus (HPV) infection and recurs frequently. Although T cells are known to play a critical role in the control of HPV infection and papilloma development, the function and phenotype of these cells in the lesion remain to be elucidated. In the present study, we examined the function and phenotype of CD4(+) T cells isolated from the lesions of primary (n = 9) and recurrent (n = 11) genital papillomas. In recurrent papillomas, the frequency of proliferating (Ki-67(+)) CD4(+) T cells was significantly reduced compared with primary papillomas. Cytokine production was evaluated by intracellular cytokine staining in anti-CD3/anti-CD28-stimulated CD4(+) T cells. CD4(+) T cells from recurrent lesions showed impaired production of IL-2, IFN-γ, and TNF-α. Of interest, the frequency of cytokine-producing CD4(+) T cells significantly correlated with the frequency of Ki-67(+)CD4(+) T cells. We also studied expression of programmed death-1 (PD-1), a T-cell exhaustion marker. The frequency of PD-1(+)CD4(+) T cells was significantly increased in recurrent lesions and inversely correlated with the frequency of cytokine-producing CD4(+) T cells. The functional significance of PD-1 expression was determined in blocking assays with anti-PD-L1, which restored cytokine production of CD4(+) T cells from recurrent lesions. Taken together, in recurrent genital papilloma lesions, proliferation, and cytokine production by CD4(+) T cells are impaired and the PD-1/PD-L1 interaction is responsible for the functional impairment of CD4(+) T cells.</P>

제마성향정기산과 열다한소탕의 항염증 효능 비교 연구

심은형 ( Eun Hyoung Shim ),유수성 ( Sooseong You ),이호영 ( Hoyoung Lee ) 대한본초학회 2017 大韓本草學會誌 Vol.32 No.4

Objectives : Jema-sunghyangjungkisan (JSGS, Jima Xingxiang Zhengqi san) and yeoldahanso-tang (YDHST, Reduo hanshao decoction) are traditional herbal formulas which commonly used to prevent and treat stroke in traditional korean medicine. However, JSGS and YDHST extracts have not been previously reported to have anti-inflammatory effects. Therefore, We measured the anti-inflammatory effects of JSGS and YDHST extracts on lipopolysaccharide (LPS)-stimulated murine macrophage cell line, RAW 264.7 cells. Methods : To investigate the anti-inflammatory activities of JSGS and YDHST extracts, tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, nitric oxide (NO) and prostaglandin E2 (PGE2) were examined in RAW 264.7 cells with LPS of 1 ㎍/㎖. Results : JSGS and YDHST extracts did not have any cytotoxicity in RAW 264.7 cells. TNF-α concentration decreased 49.67% at 500 ㎍/㎖ by JSGS but, YDHST has no statistically significant effect at all concentration. IL-6 accumulation on JSGS and YDHST extracts in LPS-stimulated RAW 264.7 cells reduced 22.03% and 41.44% at 500 ㎍/㎖ respectively. In addition, JSGS has no inhibitory effects on NO accumulation and YDHST reduced 10.08% at 500 ㎍/㎖. Moreover, JSGS and YDHST treatment does-dependently reduced the PGE2 production. In particular, YDHST (500 ㎍/㎖) extract was more effective compared with 10 ng/㎖ of indomethacin which is the PGE2 positive control. Conclusions : Our results suggest that treatment of JSGS and YDHST extracts decreased the LPS-stimulated inflammation. Therefore, in the present study, we demonstrated that JSGS and YDHS may be used as a potential anti-inflammatory therapeutic agent.

Acupuncture for in vitro fertilization in women with poor ovarian response: a systematic review

장수빈,Kyeong Han Kim,Ji Hee Jun,Sooseong You 한국한의학연구원 2020 Integrative Medicine Research Vol.9 No.2

Background: Poor ovarian response (POR) is one reason for infertility. In vitro fertilization (IVF) is frequently used to help achieve pregnancy, and performing acupuncture before IVF may promote ovulation and reduce egg retrieval pain. The purpose of this systematic review was to evaluate the effectiveness of acupuncture on clinical pregnancy rates (CPR) after IVF in women with POR. Methods: Eight electronic databases were searched in January 2020, and reference lists of retrieved articles and previous review articles were hand-searched. Randomized controlled trials (RCTs) using any type of acupuncture for women with POR undergoing IVF were considered. Risk of bias was assessed using the Cochrane risk of bias standards. Results: Three RCTs were included in this review. CPR and the number of retrieved oocytes were measured in two studies, while the values of anti-Mullerian hormone (AMH) and antral follicle count (AFC) were only reported in one study. In two studies, CPR was higher in the intervention group than the control group [37.8 % vs 24.3 %]. We did not conduct a meta-analysis, as there was a high level of heterogeneity in interventions among the included trials. Conclusions: This study suggests that acupuncture may improve CPR, AMH, AFC and the number of retrieved oocytes in women with POR undergoing IVF. However it is difficult to conclude that acupuncture is more effective than conventional treatment. Additionally, more clinical trials are needed to evaluate the effectiveness of acupuncture on CPR and other outcomes of POR. Study registration: PROSPERO CRD42018087813; https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42018087813

Concept of blood stasis in Chinese medical textbooks: A systematic review

Choi, Tae-Young,Jun, Ji Hee,Park, Bongki,Lee, Ju Ah,You, Sooseong,Jung, Jeeyoun,Lee, Myeong Soo Elsevier 2016 EUROPEAN JOURNAL OF INTEGRATIVE MEDICINE Vol.8 No.3

<P><B>Abstract</B></P> <P><B>Introduction</B></P> <P>Blood stasis is a clinical syndrome caused by an impedance or cessation of blood flow and is regarded as the cause or the result of many chronic diseases in traditional Chinese medicine (TCM). The aim of this review was to summarize definitions of blood stasis in TCM and recurring concepts regarding the physiopathology that accompany these definitions.</P> <P><B>Methods</B></P> <P>A systematic review of textbooks was performed in 5 internet bookstores (Huawen and Cathay bookshops, Taobao, Amazon, Dangdang, Baidu), 3 search engines (Google, Baidu, and Yahoo) and 2 electronic libraries (the Korea Institute of Oriental Medicine and the Beijing University of Chinese Medicine), to find unique definitions of blood stasis. The authors assessed all books, extracted unique definitions, and selected concepts of physiopathology by using the constant comparison method.</P> <P><B>Results</B></P> <P>The authors found a total of 55 textbooks. Of which, 17 was included for further analysis and identified 17 definitions of blood stasis with recurring concepts surrounding the physiopathology. These concepts were composed of 4 categories that included vascular obstruction, abnormal flow of blood, blood congestion in a viscus, and contaminated blood.</P> <P><B>Conclusion</B></P> <P>Blood stasis is still a developing concept. This information may be a valuable reference for future research seeking to standardise the concept of blood stasis.</P>

Antibody-Secreting Cells with a Phenotype of Ki-67^low, CD138^high, CD31^high, and CD38^high Secrete Nonspecific IgM during Primary Hepatitis A Virus Infection

Hong, Seokchan,Lee, Hyun Woong,Chang, Dong-Yeop,You, Sooseong,Kim, Jihye,Park, Jun Yong,Ahn, Sang Hoon,Yong, Dongeun,Han, Kwang-Hyub,Yoo, Ook Joon,Shin, Eui-Cheol The American Association of Immunologists, Inc. 2013 JOURNAL OF IMMUNOLOGY Vol.191 No.1

<P>Although studies investigating the nature of Ab-secreting cells (ASCs) during acute infection with influenza or dengue virus found that the ASC response was dominated by virus-specific IgG secretion, the Ag specificity and phenotype of ASCs during primary acute viral infection were not identified. To this end, we investigated the nature of ASCs in direct ex vivo assays from patients with acute hepatitis A caused by primary infection with hepatitis A virus (HAV). We found that the frequency of CD27<SUP>high</SUP>CD38<SUP>high</SUP> ASCs was markedly increased in the peripheral blood during the acute phase of HAV infection. Moreover, substantial numbers of ASCs were non-HAV–specific and dominantly secreted IgM. We detected HAV-specific ASCs by staining with fluorochrome-tagged HAV-VP1 protein. As compared with HAV-specific ASCs, non-HAV–specific ASCs were Ki-67<SUP>low</SUP>CD138<SUP>high</SUP>CD31<SUP>high</SUP>CD38<SUP>high</SUP>, demonstrating that non-HAV–specific ASCs had a bone marrow plasma cell–like phenotype whereas HAV-specific ASCs had a phenotype typical of circulating plasmablasts. These data suggest that non-HAV–specific ASCs might be mobilized plasma cells from the bone marrow or the spleen, whereas HAV-specific ASCs were newly generated plasmablasts. In this study, we provide evidence that pre-existing plasma cells are released into the circulation and contribute to Ag-nonspecific secretion of IgM during primary HAV infection.</P>