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( Soo Mi Kweon ),( Zee Won Lee ),( Sun Ju Yi ),( Young Myeong Kim ),( Jeong A Han ),( Sang Gi Paik ),( Kwon Soo Ha ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.2
Tissue transglutaminase (tTGase) regulates various biological processes, including extracellular matrix organization, cellular differentiation, and apoptosis. Here we report the protective role of tTGase in the cell death that is induced by the tumor necrosis factor a(TNF-α) and ceramide, a product of the TNF-αsignaling pathway, in human neuroblastoma SH-SY5Y cells. Treatment with retinoic acid (RA) induced the differentiation of the neuroblastoma cells with the formation of extended neurites. Immunostaining and Western blot analysis tTGase expression by RA treatment. TNF-αor C, ceramide, a cell permeable ceramide analog, induced cell death in normal cells, but cell death was largely inhibited by the RA treatment. The inhibition of tTGase by the tTGase inhibitors, mono-dansylcadaverline and cystamine, eliminated the protective role of RA-treatment in the cell death that is caused by TNF-α or C_(2)-ceramide, In addition, the co-treatment of TNF-α and cycloheximide decreased the protein level of tTGase and cell viability in the RA-treated cells, supporting the role of tTGase in the protection of cell death. DNA fragmentation was also induced by the co-treatment of TNF-α and cycloheximide. These results suggest that tTGase expressed by RA treatment plays an important role in the protection of cell death caused by TNF-α and ceramide.
Kweon, Soo-Mi,Lee, Zee-Won,Yi, Sun-Ju,Kim, Young-Myeong,Han, Jeong-A,Paik, Sang-Gi,Ha, Kwon-Soo Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.2
Tissue transglutaminase (tTGase) regulates various biological processes, including extracellular matrix organization, cellular differentiation, and apoptosis. Here we report the protective role of tTGase in the cell death that is induced by the tumor necrosis factor $\alpha$ (TNF-$\alpha$) and ceramide, a product of the TNF-$\alpha$ signaling pathway, in human neuroblastoma SH-SY5Y cells. Treatment with retinoic acid (RA) induced the differentiation of the neuroblastoma cells with the formation of extended neurites. Immunostaining and Western blot analysis showed the tTGase expression by RA treatment. TNF-$\alpha$ or $C_2$ ceramide, a cell permeable ceramide analog, induced cell death in normal cells, but cell death was largely inhibited by the RA treatment. The inhibition of tTGase by the tTGase inhibitors, monodansylcadaverine and cystamine, eliminated the protective role of RA-treatment in the cell death that is caused by TNF-$\alpha$ or $C_2$-ceramide. In addition, the co-treatment of TNF-$\alpha$ and cycloheximide ecreased the protein level of tTGase and cell viability in the RA-treated cells, supporting the role of tTGase in the protection of cell death. DNA fragmentation was also induced by the co-treatment of TNF-$\alpha$ and cycloheximide. These results suggest that tTGase expressed by RA treatment plays an important role in the protection of cell death caused by TNF-$\alpha$ and ceramide.
참억새로부터 분리된 S-Adenosylmethionine Synthetase (SAMS) 유전자를 이용한 담배 형질전환체계 확립
Mi Ran Jeon,Eun Soo Seong,Ji Hye Yoo,Jae Hoo Choi,Chang Heum Kim,Byeong Ju Kang,Kweon Heo,Chang Yeon Yu 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10
Background : Temperature is major factor for growth plant. Recently, because of global warming, abnormal temperature included drought, deluge, sudden temperature change and heavy snow damaged crops in the world. In Korea, crops have been sensitive to low temperature on early growth stage, e.g. fruit tree and ginseng, were damaged owing to sudden heavy snow and cold on Spring. Therefore, recently interest in cold resistance crops were increased in demand rapidly. This study was performed to establish transgenic Nicotiana benthamiana by transforming cold resistant gene related to cold tolerance S-adenosylmethionine synthetase (SAMS) isolated from Miscanthus sinensis. Methods and Results : Total RNA was extracted from leaves of M. sinensis using Trizol assay and isolated MsSAMS. Isolated MsSAMS was insert into SacⅠ- XbaⅠ sites of pMBP1 vector. The vector was transformed to Agrobacterium tumefaciens strain LBA4404 by DH5α. A. tumefaciens with binary plasmid were selected at YEP medium supplemented with kanamycin. Cut leaves of tobacco were co-cultured with selected A. tumefaciens. Co-cultured leaves was grown on regeneration medium for a month at dark condition, and transferred to at light condition. Regeneration shoot from callus were excised and transferred to root-induction medium. Approximately, 58% of leaves explant produced callus. Nearly, 30% of callus had shoot and approximately, 94% of shoots were rooted in root-induction medium. Conclusion : We established an efficient transformation system of N. benthamiana transformed by using MsSAMS gene related to cold tolerance isolated from M. sinensis. We may use the produced transgenic plants to prevent damages carried by cold.
Development of End-spliced Dimeric Nanodiscs for the Improved Virucidal Activity of a Nanoperforator
Mi Soo KIM,Hyunseok OH,Seokoh MOON,Soomin KIM,Jeonghui MOON,NaYeon KIM,Dae-Hyuk KWEON 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Lipid-bilayer nanodiscs (NDs) wrapped in membrane scaffold proteins (MSPs) have primarily been used to study membrane proteins of interest in a physiological environment. Recently, NDs have been employed in broader applications including drug delivery, cancer immunotherapy, bio-imaging, and therapeutic virucides. Here, we developed a method to synthesize a dimeric nanodisc, whose MSPs are circularly end-spliced, with long-term thermal stability and resistance to aggregation. The end-spliced nanodiscs (esNDs) were assembled using MSPs that were self-circularized inside the cytoplasm ofEscherichia colivia highly efficient protein trans-splicing. The esNDs demonstrated a consistent size and 4-5-fold higher stability against heat and aggregation than conventional NDs. Moreover, cysteine residues on trans-spliced circularized MSPs allowed us to modulate the formation of either monomeric nanodiscs (essNDs) or dimeric nanodiscs (esdNDs) by controlling the oxidation/reduction conditions and lipid-to-protein ratios. When the esdNDs were used to prepare an antiviral nanoperforator that induced the disruption of the viral membrane upon contact, antiviral activity was dramatically increased, suggesting that the dimerization of nanodiscs led to cooperativity between linked nanodiscs. We expect that controllable structures, long-term stability, and aggregation resistance of esNDs will aid the development of novel versatile membrane-mimetic nanomaterials with flexible designs and improved therapeutic efficacy.
Kweon, Mi-Na,Ryu, Hong-Soo,Mun, Jeung-Hye 부경대학교 기초과학연구소 1993 기초과학연구논문집 Vol.4 No.-
두부 제조공정 중의 부산물인 비지의 효율적인 이용을 위하여, 원료 대두 중량의 10%에 해당하는 건조비지를 첨가시킨 두부를 제조하여 이의 영양적 품질을 검토하였다. 일반성분은 수분의 경우 건조비지 첨가 두부가 80.6%, 일반 두부가 77.5%로써 비지 첨가 두부가 다소 높았으며 단백질의 함량은 건조비치 첨가 두부와 일반 두부 각각 38.5%, 45.8%였으며, 지질의 함량은 각각 34.3%, 26.9%로써, 단백질과 지질 함량이 낮은 건조비지 첨가로 인해 일반 두부에 비해 건조비지 첨가 두부가 낮은 함량을 보였다. Trypsin inhibitor(TI) 함량은 비지의 경우 잔존량이 6.9(mg/g시료)정도였고, 건조비지의 경우에도 거의 비슷하였다. 건조비지 첨가 두부에는 원료 대두에 있었던 TI의 12% 정도만 남아있었다. 단백질의 효소소화율(enzyme digestibility)은 비지, 건조비지 각각 87%, 86% 정도로 높았고 건조비지 첨가 두부도 일반 두부와 거의 동일한 91%정도였다. 효소소화율은 TI함량이 저하함에 따라 역상관관계를 가지면서 상승하였다. 구성 아미노산 조성은 일반 두부와 건조비지 첨가 두부는 거의 차이가 없었으며, 총아미노산에 대한 필수 아미노산의 비율은 일반 두부가 40.4%, 비지첨가 두부가 40.6%였으며, lysine 함량이 높은 건조비지로 인하여 건조비지 첨가 두부는 lysine의 보충 효과가 있었다. 단백품질을 Computed Protein Efficiency Ratio(C-PER)로 계산할 때 일반 두부는 1.95, 비지첨가두부는 1.4였으나 Discriminant Computed Protein Efficiency Ratio(DC-PER)로 계산할 때는 오히려 비지 첨가 두부경우가 높았다. 여러 연구자들의 대두가공품에 대한 생체실험 결과와 비교할 때 일반 두부를 제외하고는 두유, 비지 및 비지첨가 두부의 영양평가는 DC-PER 방법이 유리한 것으로 생각되었다. The effect of dried soymilk residue(DSR) on protein quality of tofu was studied. The amount of added DSR into soybean water extract was corresponding 10% (dry basis) of soybean used in tofu manufacturing. Proximate composition and in vitro protein qualities of soybeans at different stages of the conversion into tofu have also been investigated. Partially substituted tofu with DSR (TDSR) had higher moisture content (80.6%) than that of tofu prepared in traditional manner (TT). TDSR contained lower content of protein (38.9%) and total lipid (26.9%) compared to 45.8% of protein and 34.3% of total lipid for TT. A large amount of trypsin inhibitor (TI) in raw soybeans was diminished and extracted through tofu processing, and only 10-1 3%) of T I in raw soybean remained in both tofu products (TDSR and 11). There was not a considerable difference in amino acid profiles between TT and TDSR, but TDSR had a higher content of lysine than that in TT. In witro studies showed that TDSR and TT were comparable in terms of both in vitro digestibilities (90% over for four-enzyme digestibility and predicted digestibility) and discriminant computed protein efficiency ratio (2.07-2.1 4, DC-PER). Unlike those in vitro indices for protein quality, computed protein efficiency rdtio (C-PER) of TDSR was much lower(l.4) than that of TT(1.95). It was revealed that C-PERs of tofu products were not in agreement with rat-PERs (1.7-1.9) in previous reports except for TT. However, DC-PER assay was more recommendable for protein quality of tofu products than C-PER assay.
Isolation, characterization, and evaluation of Bacillus thuringiensis isolated from cow milk
Kweon, Chang-Hee,Choi, Sang-Yoon,Kwon, Hyog-Young,Kim, Eun-Hye,Kang, Hyun-Mi,Moon, Jin-San,Jang, Geum-Chag,Lee, Hee-Soo,Kang, Seung-Won,Kim, Jong-Man,Pyo, Suhkneung,Rhee, Dong-Kwon The Korean Society of Veterinary Science 2012 大韓獸醫學會誌 Vol.52 No.3
Probiotics colonize the intestines and exert an antibacterial effect on pathogens. Therefore, probiotics could be used as a preventive agent against lethal infections. To isolate probiotic microorganisms, 116 bacterial strains were isolated from healthy cow's milk and were subjected to Gram-stain, morphology and biochemical analyses, Vitek analysis, and 16S rRNA analysis. One of the strains identified as Bacillus (B.) thuringiensis 87 was found to grow very well at pH 4.0~7.0 and to be resistant to high concentrations of bile salts (0.3~0.9% w/v). B. thuringiensis was susceptible to the antibiotics used in the treatment of bovine mastitis, yet it exhibited an antimicrobial effect against Staphylococcus (S.) aureus 305. Moreover, it protected mice from experimental lethal infections of E. coli O55, Salmonella typhimurium 01D, and S. aureus 305 through a significant induction of interferon-${\gamma}$, even at four-week post-administration of B. thuringiensis. Although oral administration of B. thuringiensis 87 did not provide significant protection against these lethal challenges, these results suggest that B. thuringiensis 87 could be a feasible candidate as a probiotic strain.