Evaluation of Metabolic Stability of Kinsenoside, an Antidiabetic Candidate, in Rat and Human Liver Microsomes

Rehman, Shaheed Ur,Kim, n Sook,Choi, Min Sun,Luo, Zengwei,Yao, Guangming,Xue, Yongbo,Zhang, Yonghui,Yoo, Hye Hyun Korean Society for Mass Spectrometry 2015 Mass spectrometry letters Vol.6 No.2

Kinsenoside is a principle bioactive compound of Anoectochilus formosanus. It exhibits various pharmacological effects such as antihyperglycemic, antioxidant, anti-inflammatory, immunostimulating, and hepatoprotective activities and has recently been developed as an antidiabetic drug candidate. In this study, as part of an in vitro pharmacokinetic study, the stability of kinsenoside in rat and human liver microsomes was evaluated. Kinsenoside was found to have good metabolic stability in both rat and human liver microsomes. These results will provide useful information for further in vivo pharmacokinetic and metabolism studies.

Migration of epoxidized soybean oil from polyvinyl chloride/polyvinylidene chloride food packaging wraps into food simulants

Choi, Min Sun,Rehman, Shaheed Ur,Kim, Hyeon,Han, Sang Beom,Lee, Jeongmi,Hong, Jongki,Yoo, Hye Hyun Springer-Verlag 2018 Environmental science and pollution research inter Vol.25 No.5

<P>Epoxidized soybean oil (ESBO) has been used in polyvinyl chloride (PVC)/polyvinylidene chloride (PVDC) food packaging cling film as a plasticizer and stabilizer. The aim of this study was to investigate the migration of ESBO from PVC/PVDC cling film, based on gas chromatography mass spectrometry (GC-MS). The specific migration of ESBO was evaluated using various food simulants (water, 4% acetic acid, 50% ethanol and n-heptane) for PVC and PVDC wrap products. ESBO did not migrate into water and 4% acetic acid for all the tested samples. However, it was released into 50% ethanol and n-heptane in several PVC/PVDC wraps, with maximum migration levels of 38.4 +/- 0.7 and 37.4 +/- 0.8 mu g/mL, respectively. These results demonstrate that ESBO is capable of being released from PVC/PVDC wrap into amphiphilic/oily food and its migration should be regularly monitored.</P>

Evaluation of Herb–Drug Interactions of <i>Hovenia dulcis</i> Fruit Extracts

Park, Jong Suk,Rehman, Shaheed Ur,Kim, In Sook,Choi, Min Sun,Na, Chun-Soo,Yoo, Hye Hyun Medknow PublicationsMedia Pvt Ltd 2017 Pharmacognosy magazine Vol.13 No.50

<P><B>Background:</B></P><P><I>Hovenia dulcis</I> (Rhamnaceae) fruits are popularly used as herbal medicines or dietary supplements in Asian countries due to functions such as liver protection and detoxification from alcohol poisoning. Accordingly, it is very likely for dietary supplemental products, including <I>H. dulcis</I> fruit extracts, to be taken with prescription drugs.</P><P><B>Objective:</B></P><P>In this study, possible food–drug interactions involving <I>H. dulcis</I> fruit extracts were evaluated based on the inhibition of cytochrome P450 (CYP) enzyme activity.</P><P><B>Material and Methods:</B></P><P>The water extract of <I>H. dulcis</I> fruit extracts was incubated in human liver microsomes with CYP-specific substrates. The formation of the CYP-specific metabolites was measured using liquid chromatography-tandem mass spectrometry.</P><P><B>Results:</B></P><P><I>H. dulcis</I> fruit extracts showed negligible effects on seven CYP isozyme activities at all concentrations tested.</P><P><B>Conclusion:</B></P><P>This result suggests that <I>H. dulcis</I> fruit extracts may have minimal pharmacokinetic interactions with coadministered drugs through the modulation of CYP enzymes.</P><P><B>SUMMARY</B></P><P><P>Food-drug interactions involving <I>H. dulcis</I> fruit extracts were evaluated.</P><P>The inhibition of CYPs by <I>H. dulcis</I> extracts was tested.</P><P><I>H. dulcis</I> extracts showed negligible effects on CYP activities.</P><P><I>H. dulcis</I> extracts may have minimal pharmacokinetic interactions with co-administered drugs.</P></P> >[FIG OMISSION]</BR><P><B>Abbreviations Used:</B> CYP: cytochrome P450 enzymes, HPLC: High performance liquid chromatography, LC-MS/MS : liquid chromatography-tandem mass spectrometry, MRM: multiple-reaction monitoring</P>

Development of a mixed-mode chromatography with tandem mass spectrometry method for the quantitative analysis of 23 underivatized amino acids in human serum

Choi, Min Sun,Rehman, Shaheed Ur,Kim, In Sook,Park, Hi-Joon,Song, Mi-Yeon,Yoo, Hye Hyun Elsevier 2017 Journal of pharmaceutical and biomedical analysis Vol.145 No.-

<P><B>Abstract</B></P> <P>In this study, a robust, selective and simplified method was developed and validated for the simultaneous quantitative analysis of 23 underivatized amino acids in human serum using mixed-mode chromatography with tandem mass spectrometry (LC–MS/MS). Serum samples were deproteinized with acetonitrile and subjected to LC–MS/MS analysis. The chromatographic separation of amino acids was achieved using a mixed-mode column (150×3mm, 3μm) with a gradient elution system; the mobile phase consisted of 50mM ammonium formate and 0.1% formic acid in acetonitrile. The total run time was 22min. Eluted compounds were detected in the electrospray ionization-positive mode with multiple reaction monitoring. The validation study evaluated linearity, repeatability, intra and inter-day accuracy and precision, and matrix effect. The validation results were satisfactory in all the tested parameters. This method was successfully applied to the analysis of amino acids in the clinical sample of human serum.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A simultaneous quantitative method for underivatized amino acids in human serum was developed. </LI> <LI> In this method, mixed-mode chromatography with tandem mass spectrometry was used. </LI> <LI> The analytical method validation was properly performed. </LI> <LI> This method was applied to the analysis of amino acids in the clinical samples of human serum. </LI> </UL> </P>

Interactions between herbs and antidiabetics: an overview of the mechanisms, evidence, importance, and management

유혜현,Kevin Kyungsik Choe,Shaheed Ur Rehman,최민선 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.7

Complementary and alternative therapies are quickly gaining importance because they are perceived to be free of side effects due to their natural origin. However, herbal remedies are complex mixtures of bioactive entities, which may interact with prescription drugs through pharmacokinetic or pharmacodynamic mechanisms and sometimes result in life-threatening consequences. In particular, diabetes patients are often treated with multiple medications due to different comorbidities, and such patients use antidiabetic medications for their entire lives; thus, it is important to make the public aware of herb interactions with antidiabetic drugs. In this paper, we summarize the reports available on the interaction of herbal remedies with oral hypoglycemic agents and describe mechanisms, preclinical or clinical evidence, importance, and management strategies.

Quantification of Three Prohibited Anabolic-Androgenic Steroids in Equine Urine using Gas Chromatography-Tandem Mass Spectrometry

Young Beom Kwak,Shaheed Ur Rehman,Hye Hyun Yoo 사단법인 한국질량분석학회 2023 Mass spectrometry letters Vol.14 No.3

Anabolic-androgenic steroids (AAS) are used illegally to enhance muscle development and increase strength and power. In this study, a reliable, and sensitive quantitative method was developed and validated using heptafluorobutyric acid anhydride (HFPA) derivatives for the simultaneous detection of prohibited AAS (testosterone [TS], boldenone [BD], 5α-estrane- 3β,17α-diol [EAD]) using gas chromatography-tandem mass spectrometry (GC-MS/MS). For processing the samples, solid phase extraction, methanolic hydrolysis, and liquid-liquid extraction were used. For detection using mass spectrometry, the mul- tiple reaction monitoring (MRM) mode was used with the electron ionization (EI) positive mode. The method was evaluated for selectivity, linearity, lower limit of quantification, intra- and inter-day precision, accuracy, and stability. The results showed that the method was accurate and reproducible for the quantitation of the three steroids. The developed method was finally applied to the analysis of a suspect gelding urine sample received from the Asian Quality Assurance Program (AQAP).

<i>In Vitro</i> Evaluation of the Effects of <i>Eurycoma longifolia</i> Extract on CYP-Mediated Drug Metabolism

Han, Young Min,Kim, In Sook,Rehman, Shaheed Ur,Choe, Kevin,Yoo, Hye Hyun Hindawi Publishing Corporation 2015 Evidence-based Complementary and Alternative Medic Vol.2015 No.-

<P><I>Eurycoma longifolia</I> (Simaroubaceae) is a popular folk medicine that has traditionally been used in Southeast Asia as an antimalarial, aphrodisiac, antidiabetic, and antimicrobial and in antipyretic remedies. This study evaluates the effects of <I>Eurycoma longifolia</I> extract on cytochrome P450 (CYP) enzyme-mediated drug metabolism to predict the potential for herb-drug interactions. Methanolic extract of <I>E. longifolia </I> root was tested at concentrations of 1, 3, 10, 30, 100, 300, and 1000 <I>µ</I>g/mL in human liver microsomes or individual recombinant CYP isozymes. The CYP inhibitory activity was measured using the cocktail probe assay based on liquid chromatography-tandem mass spectrometry. <I>E. longifolia</I> showed weak, concentration-dependent inhibition of CYP1A2, CYP2A6, and CYP2C19. The inhibitory effects on these CYP isozymes were further tested using individual recombinant CYP isozymes, showing IC<SUB>50</SUB> values of 324.9, 797.1, and 562.9 <I>μ</I>g/mL, respectively. In conclusion, <I>E. longifolia</I> slightly inhibited the metabolic activities of CYP1A2, CYP2A6, and CYP2C19 but this issue requires careful attention in taking herbal medicines or dietary supplements containing <I>E. longifolia</I> extracts.</P>

Magnetocaloric effect and magnetic properties of the isovalent Sr²⁺ substituted Ba₂FeMoO₆ double perovskite

Hussain, Imad,Anwar, M.S.,Khan, S.N.,Shahee, Aga,Ur Rehman, Zeeshan,Heun Koo, Bon Elsevier 2017 CERAMICS INTERNATIONAL Vol.43 No.13

<P><B>Abstract</B></P> <P>Fine-tuning the charge distribution in Ba<SUB>2</SUB>FeMoO<SUB>6</SUB> obtained via “isovalent” substitution at the A-site (i.e., Ba) is expected to bring about changes in the physical properties of the system that can be manipulated in magnetic refrigerants. With this motivation, the phase formation, crystal structure, microstructure, magnetic and magnetocaloric properties of the Ba<SUB>2−x</SUB>Sr<SUB>x</SUB>FeMoO<SUB>6</SUB> (0≤x≤0.4) samples fabricated by solid state reaction method have been investigated. The X-ray diffraction analysis confirmed the formation of cubic structure with <I>Fm</I>3<I>m</I> space group in all the fabricated samples. The magnetization measurements and Arrott analysis revealed a second order of ferromagnetic phase transition in all the samples. An increase in magnetization and Curie temperature (T<SUB>C</SUB>) was observed with the increase in Sr-content that was attributed to the increased orbital hybridization and exchange interaction between Fe and Mo ions. The magnitude of the maximum magnetic entropy change at the Curie temperature and the relative cooling power were observed to slightly decrease with the increased Sr doping. The excellent magnetocaloric features and convenient adjustment of Curie temperature make these materials useful for magnetic refrigeration in a wide range of temperature.</P>

Evaluation of Metabolic Stability of Kinsenoside, an Antidiabetic Candidate, in Rat and Human Liver Microsomes

Hye Hyun Yoo,Yonghui Zhang,Yong Bo Xue,Guangming Yao,Zengwei Luo,Min Sun Choi,In Sook Kim,Shaheed Ur Rehman 사단법인 한국질량분석학회 2015 Mass spectrometry letters Vol.6 No.2

Kinsenoside is a principle bioactive compound of Anoectochilus formosanus. It exhibits various pharmacological effects such as antihyperglycemic, antioxidant, anti-inflammatory, immunostimulating, and hepatoprotective activities and has recently been developed as an antidiabetic drug candidate. In this study, as part of an in vitro pharmacokinetic study, the stability of kinsenoside in rat and human liver microsomes was evaluated. Kinsenoside was found to have good metabolic stability in both rat and human liver microsomes. These results will provide useful information for further in vivo pharmacokinetic and metabolism studies.