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RISS 인기검색어
- 검색키워드
- 저자 : ( Seung-joo Jekal ) (검색결과 57 건)
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최영자,제갈승주,윤영승,김재만 木浦大學校 基礎科學硏究所 2001 基礎科學硏究誌 Vol.19 No.-
Mast cells reside in connective tissue and mucous tissue that encircle the internal environment of the body, where they mediate inflammatory response encountering foreign materials. Mast cells are also found in uterus tissue which is, in fact, exposed to external world. Because the cells are provoked by invading foreign cells to cause inflammatory response, their response in the uterus should be controled for success in fertilization and implantation, in that the foreign sperms and fertilized egg are willing to invade into the body. In order to confirm such assumption, we investigated the changes in mast cell numbers and their contents in the rat uterus during the estrus cycle and related these to the changes in sex-hormone concentration. The mean number of mast cell at the proestrus and estrus phase was 4.8±2.72 and 5.98±1.55. respectively. The number increased as much as 3 times more then previous phase at the metestrus phase and subsided to half at following the diestrus phase. Those mast cells were subdivided type. Connective tissue type was major at metestrus and diestrus phase. At the following proestrus, however, the muscous type was largely increased and major population was changed to muscous and intermediate type at estrus phase. In the immature uterus of young female, as few as 1.67±0.23 cells were detected and most of them were mucous and intermediate type. The concentration of circulating estradiol-17 β was lowest at the metestrus phase both in free and bound form. The results suggest that estrogen may be involved in inhibiting swarming of mast cells in the uterus and accumulating the contents of secretory vesicles.
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Jekal, Seung-Joo,Lee, Jae-Hyoung,Park, Seung-Teack 대한임상검사과학회 2012 대한임상검사과학회지(KJCLS) Vol.44 No.4
Cyclooxygenase(COX-2) is an inducible enzyme that catalyzes the synthesis of prostaglandins (PGs) from arachidonic acid. Over-expression of COX-2 has been reported to be associated with progressive hepatic fibrosis in chronic hepatic C infection and rat liver fibrosis induced by carbon tetrachloride($CCl_4$). Recently, it is well known that mast cell products can stimulate the proliferation of hepatic stellate cells and key players in liver fibrosis. But little is known regarding their role in $CCl_4$-induced liver fibrosis in rat. Our aim was to investigate the relation between COX-2 expression and mast cells during liver fibrosis after $CCl_4$ treatment. Thirty Wistar rats were divided into five groups (non-treated 0, 2, 4, 6 and 8-week after $CCl_4$-treatment). Reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry were used to assess the expression of ${\alpha}$-smooth muscle actin (${\alpha}$-SMA), collagen-1 and COX-2 in liver tissue from $CCl_4$-treated rats. The density of collagen and mast cells were determined using a computerized image analysis system in liver sections stained with picrosirius red and toluidine blue, respectively. The expression levels of ${\alpha}$-SMA, collagen-1 and COX-2 mRNA were significantly higher at 2 wk in $CCl_4$-treated groups than non-treated group. The number of mast cells in liver tissues increased gradually from 2 wk to 6 wk depending on the fibrosis severity but decreased abruptly at 8 wk. The significant increase of collagen-1 and ${\alpha}$-SMA mRNA expression in $CCl_4$-treated rats was continued until 6 wk while the COX-2 mRNA was significantly decreased at 8 wk. These results suggest that increased mast cells are closely associated with COX-2 over-expression during hepatic fibrogenesis of $CCl_4$-treated rats.
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Seung-Joo Jekal(제갈승주),Pil Seung Kwon,Jin Kyung Kim 대한의생명과학회 2010 Biomedical Science Letters Vol.16 No.4
The purpose of this study was to clarify the effect of light emitting diode (LED) irradiation on healing of impaired wound and alteration of mast cells in experimental diabetic rats. Twenty-four male Sprague-Dawley rats were divided into four groups: excision (Ex), excision-LED irradiation (Ex-LED), diabetes + excision (DM) and diabetes + excision + LED irradiation (DM-LED). Diabetes was induced in rats by streptozotocin (STZ) injection (70 mg/kg, single dose) and 6 ㎜ punch excision wounds were created on the back after shaving hair. The LED-irradiated rats were treated to a daily dose of 5 J/㎠ LED (630 nm) light for 11 days after surgery, and were killed at day 1, 3, 7 and 11. The lesion and adjacent skin tissues were excised, fixed with 10% buffered formalin and embedded with paraffin. For evaluation of wound healing, hematoxylin-eosin (HE) and Masson trichrome staining were performed. Mast cells (MCs) were stained with toluidine blue (pH 0.5) and quantified using a computerized image analysis system. The proliferation activity of keratinocyte in skin tissues was analyzed on sections immunostained with proliferative cell nuclear antigen (PCNA). The results showed that wound healing rate, collagen density and neo-epidermis length, number of PCNA-positive cells, fibroblasts and mast cells were significantly higher in the LED-irradiated rats than in the DM and Ex rats throughout the periods of experiment. Exceptionally, the number of MCs was significantly lower at day 11 compared with day 7 after surgery in the all groups. These findings suggest that the LED irradiation may promote the tissue repair process by accelerating keratinocyte and fibroblast proliferation and collagen production in normal rats as well as in diabetic rats, and MCs may play an important role at an early stage of skin wound healing in normal and diabetic rats.
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( Seung-joo Jekal ),( Jae-hyoung Lee ),( Seung-teack Park ) 대한임상검사과학회 2012 대한임상검사과학회지(KJCLS) Vol.44 No.4
Cyclooxygenase(COX-2) is an inducible enzyme that catalyzes the synthesis of prostaglandins (PGs) from arachidonic acid. Over-expression of COX-2 has been reported to be associated with progressive hepatic fibrosis in chronic hepatic C infection and rat liver fibrosis induced by carbon tetrachloride(CCl4). Recently, it is well known that mast cell products can stimulate the proliferation of hepatic stellate cells and key players in liver fibrosis. But little is known regarding their role in CCl4-induced liver fibrosis in rat. Our aim was to investigate the relation between COX-2 expression and mast cells during liver fibrosis after CCl4 treatment. Thirty Wistar rats were divided into five groups (non-treated 0, 2, 4, 6 and 8-week after CCl4-treatment). Reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry were used to assess the expression of α-smooth muscle actin (α-SMA), collagen-1 and COX-2 in liver tissue from CCl4-treated rats. The density of collagen and mast cells were determined using a computerized image analysis system in liver sections stained with picrosirius red and toluidine blue, respectively. The expression levels of α-SMA, collagen-1 and COX-2 mRNA were significantly higher at 2 wk in CCl4-treated groups than non-treated group. The number of mast cells in liver tissues increased gradually from 2 wk to 6 wk depending on the fibrosis severity but decreased abruptly at 8 wk. The significant increase of collagen-1 and α-SMA mRNA expression in CCl4-treated rats was continued until 6 wk while the COX-2 mRNA was significantly decreased at 8 wk. These results suggest that increased mast cells are closely associated with COX-2 over-expression during hepatic fibrogenesis of CCl4-treated rats.
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자궁경부의 편평상피내병소와 편평상피세포암종에서의 비만세포에 관한 연구
제갈승주 ( Seung Joo Jekal ),차현희 ( Hyun Hee Cha ),최영자 ( Young Ja Choi ),노종섭 ( Jong Sup Roh ),곽효일 ( Hyo Il Kwak ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2
Mast cells have recently been found to be well correlated with the inhibition of invasiveness and metastasis in various cancer. This work aimed to investigate the number of mast cells and ratio of mast cell granulation in squamous intraepithelial lesions and squamous cell carcinoma of the cervix. Cervical biopsy specimens were divided into low grade squamous intraepithelial lesion(n=20), high grade squamous intraepithelial lesion(n=75) and squamous cell carcinoma(n=40) by The Bethesda System. The sections were cut from routinely formalin- f1xed and paraffin-embedded tissues and stained with 0.5% toluidine blue(pHO.5)-nuc1ear fast red. The mast cells were quantif1cated the numbers per square millimeter using a computerized image analysis system. Mean number of mast cells was signif1cantly higher in high grade squamous intraepithelial lesion than in low grade squamous intraepithelial lesion and signif1cantly lower in squamous cell carcinoma than in high grade squamous intraepithelial lesion(p<0.01). The percent of mast cell ganulation also was significantly higher in high grade squamous intraepithelial lesion than in low grade squamous intraepithelial lesion and significantly lower in squamous cell carcinoma than in high grade intraepithelial lesion (p<0.01). These results suggested that mast cell number and percent of mast cell granulation were to be correlated to the development from precancerous lesion to cervical cancerous lesion and may be useful as markers of cervical squamous cell carcinoma.
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도은염색법중(鍍銀染色法中) Slide 부착법(附着法)과 절편부유법(切片浮遊法)의 비교고찰(比較考察)
제갈승주 ( Jekal Seung Joo ),오근영 ( Keun Young Oh ) 대한임상검사과학회 1979 대한임상검사과학회지(KJCLS) Vol.11 No.1
Two kinds of silver impregnation methods are most widely used in routine histopathological laboratory. that is frozen section which requires careful handling, and processing and produces inconstant result and uncomfortable procedure, in addition the section is easily or frequently detached from the slide due to high alkalinity of silver particles. This may be overcome by carrying out steps of the procedure . by floating the paraffin section on the solutions. The procedure shows simple and no detachment from the slide, also it can get a clear background by deparaffinization.
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스트렙토조토신 유도 당뇨 흰쥐에서 전기자극이 상처치유와 피부 비만세포에 미치는 영향
제갈승주 ( Seung Joo Jekal ),이경선 ( Kyung Sun Lee ),정옥봉 ( Ok Bong Chung ),이재형 ( Jae Hyoung Lee ) 대한임상검사과학회 2008 대한임상검사과학회지(KJCLS) Vol.40 No.2
The aim of this study was to investigate the effect of electrical stimulation on healing of impaired wound and alteration of mast cells in experimental diabetic rats. Thirty male Sprague-Dawley rats were divided into three groups : incision (control), diabetes+incision (diabetes) and diabetes + incision + electrical stimulation (D/ES). Diabetes was induced in rats by streptozotocin (STZ) injection (60 mg/kg, one time) and 20 mm length incision wounds were created on the back after shaving hair. The electrical stimulation rats were treated with a current intensity of 30~50 V at 120 pps and 140 μs for 10 days from 3 days after STZ injection. The lesion and adjacent skin tissues were fixed with 10% buffered formalin, embedded with paraffin. For wound healing analysis, hematoxylin-eosin (HE) and picrosirius red staining were performed. Mast cells (MC) were stained with toluidine blue (pH 0.5) and quantified at ×200 using a light microscope. The density of keratinocyte proliferation and microvessels in skin tissues were analyzed using a computerized image analysis system on sections immunostained with proliferative cell nuclear antigen (PCNA) and α-smooth muscle actin (α-SMA), respectively. The results showed that the wound healing rate, collagen density and neoepidermis thickness, density of PCNA-positive cells and density of α-SMA-positive vessels were significantly higher in D/ES rats than in diabetic rats. The density of MCs and degranulated MCs in D/ES rats were also significantly higher than those in diabetic rats. These findings suggest that the electrical stimulation may promote the tissue repair process by accelerating collagen production, keratinocyte proliferation and angiogenesis in the diabetic rats, and MCs are required for wound healing of skin in rats.
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파라핀포매조직에서의 톨루이단블루염색에 의한 초기와후기 아포토세포의 감별
제갈승주 ( Seung Joo Jekal ),차현희 ( Hyun Hee Cha ) 대한임상검사과학회 1998 대한임상검사과학회지(KJCLS) Vol.30 No.3
Apoptosis is a morphologically distinct form of programmed cell death that plays a key role in the growth regulation of a variety of tissues. Several techniques based on the detection of DNA fragmentation as an important characteristic of this process have been established Since their methods, however, are expensive, time-consuming, and poor tissue morphology, it can be difficulty to apply in routine histological sections. The aim of this study was performed to determine the usefulness of toluidine blue staining in Camoy-fixed and paraffin-embedded tissues. The samples from spleen, small intestine, liver, kidney and testis was evaluated. We compared the toluidine blue(TB) staining with the hematoxylin and eosin (HE) and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling(11JNEL) staining used to detection of apoptotic cells. The distribution of apoptotic cells in five tissues was similar to the site of previous studies. The apoptotic cells are classified as early and late phase based on their morphology. In HE sections, apoptosis was usually recognizable by the presence of apoptotic bodiestlate phase). Although the early phase of apoptosis can be detected as a cells possessing an nucleus with irregular contour and condensed chromatin magination at nuclear periphery in HE sections, it was difficult to detect and was easily overlooked. In TUNEL sections, apoptosis appeared intense staining mainly in late phase and weak staining in early phase. However, the colorized signals of TUNEL are much easier to detect than apoptotic bodies in HE sections. In TB staining, apoptotic cells revealed with metachromatic staining in early Phase and late phase with hyperchromatic staining, though the intensity of metachromatic staining is less weakened than positive signals in TUNEL it can be discriminated between early and late apoptotic cells. Thus, if the metachromatic intensity of TB, in future, could be more improved, it may be a cheap, convenient, useful technique for detection of apoptosis as well as for discrimination of early and late apoptotic cells in Carnoy-fixed and paraffin-embedded tissues.
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