http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Seul Ah Lee,Mi Suk Choi,Bo-Ram Park,Jin-Soo Kim,Chun Sung Kim 대한구강생물학회 2021 International Journal of Oral Biology Vol.46 No.4
Nypa fruticans Wurmb (NFW) contains a large amount of phenolic acid and flavonoids, and is popular as a superfood in Myanmar. NFW has various biological activities, such as anti-inflammatory, anti-oxidant, and neuroprotective properties; however, the anti-cancer effect of NFW have not been reported. In this study, we investigated the anticancer activity of water extracts of NFW (WeNFW) and the underlying mechanism in human FaDu hypopharyngeal squamous carcinoma cells. The WeNFW inhibited FaDu cell growth in a dose-dependent manner without affecting normal cells (L929), as determined by an MTT assay and Live and Dead assay. In addition, the concentrations of WeNFW without cytotoxicity (0.025, 0.05, and 0.1 mg/mL) inhibited wound healing and colony formation. Furthermore, WeNFW significantly induced apoptosis through the proteolytic cleavage of caspase-3 and -9, poly (ADP-ribose) polymerase, and downregulation of Bcl-2 and upregulation of Bax in FaDu cells, as determined by DAPI staining, FACS analysis, and western blot analysis. Taken together, these results suggest that WeNFW exhibits potent anti-cancer effects by suppressing the growth of oral cancer cells, wound healing and colony formation activity. Via mitrochondrial-dependent apoptotic pathways in human FaDu hypopharyngeal squamous carcinoma cells. Therefore, WeNFW can provide a natural chemotherapeutic drug for oral cancer in humans.
Seul Ah Lee,Bo-Ram Park,Sung Min Moon,Do Kyung Kim,Chun Sung Kim 대한구강생물학회 2018 International Journal of Oral Biology Vol.43 No.2
Codium fragile (Suringar) Hariot is an edible green seaweed that belong to the Codiaceae family and has been used in Oriental medicine for the treatment of enterobiasis, dropsy, and dysuria. Methanol extract of codium fragile has anti-oxidant, anti-inflammatory and anti-cancer properties, although the anti-cancer effect on oral cancer has not yet been reported. In this study, we investigated the anti-cancer activity and the mechanism of cell death by methanol extracts of Codium fragile (MeCF) on human FaDu hypopharyngeal squamous carcinoma cells. Our data showed that MeCF inhibits cell viability in a dose-dependent manner, and markedly induced apoptosis, as determined by the MTT assay, Live/Dead assay, and DAPI stain. In addition, MeCF induced the proteolytic cleavage of procaspase -3, -7, -9 and poly(ADP-ribose) polymerase(PARP), and upregulated or downregulated the expression of mitochondrial-apoptosis factor, Bax(pro-apoptotic factor), and Bcl-2(anti-apoptotic factor), . Futhermore, MeCF induced a cell cycle arrest at the G1/S phase through suppressing the expression of the cell cycle cascade proteins, p21, CDK4, CyclinD1, and phospho-Rb. Taken together, these results indicated that MeCF inhibits cell growth, and this inhibition is mediated by caspase- and mitochondrial-dependent apoptotic pathways through cell cycle arrest at the G1/S phase in human FaDu hypopharyngeal squamous carcinoma cells. Therefore, methanol extracts of Codium fragile can be provided as a novel chemotherapeutic drug due to its growth inhibition effects and induction of apoptosis in human oral cancer cells.
Kim, Seul-Ah,Jang, Ye-Ji,Heo, Ji Eun,Li, Ling,Moon, Jin Seok,Han, Nam Soo Elsevier 2018 Journal of biotechnology Vol.287 No.-
<P><B>Abstract</B></P> <P> <I>Leuconostoc citreum</I> is an important lactic acid bacterium used as a starter culture for producing kimchi, the traditional Korean fermented vegetables. An efficient host strain for plasmid transformation, <I>L. citreum</I> EFEL2700, was isolated from kimchi, and it has been frequently used for genetic engineering of <I>L. citreum</I>. In this study, we report the whole genome sequence of the strain and its genetic characteristics. Genome assembly yielded 5 contigs (1 chromosome and 4 plasmids), and the complete genome contained 1,923,830 base pairs (bp) with a G + C content of 39.0%. Average nucleotide identity analysis showed high homology (≥ 99%) to the reference strain <I>L. citreum</I> KM 20. The smallest plasmid (4.3 kbp) was used as an <I>Escherichia coli</I> shuttle vector (pCB) for heterologous gene expression, and <I>L. citreum</I> EFEL2700 showed the highest transformation efficiency, 6.7 × 10⁴ CFU μg<SUP>−1</SUP> DNA. Genetic analysis of the genome enabled the construction of primary metabolic pathway showing a typical hetero-type lactic acid fermentation. Notably, no core genes for primary metabolism were observed in plasmid 4 and it could be eliminated to create an efficient host for gene transformation. This report will facilitate the understanding and application of <I>L. citreum</I> EFEL2700 as a food-grade microbial cell factory.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>L. citreum</I> EFEL2700 is an efficient host for pCB vector transformation. </LI> <LI> It has 1 chromosome and 4 plasmids with 1.92 Mb size genome and 39% G + C content. </LI> <LI> Four plasmids have no genes involved in primary metabolism, thereby sequential elimination of plasmids may result in a higher transformation efficiency. </LI> <LI> This report will facilitate the understanding and application of <I>L. citreum</I> as a food-grade microbial cell factory. </LI> </UL> </P>
MicroRNA-203 Induces Apoptosis by Targeting<i>Bmi-1</i>in YD-38 Oral Cancer Cells
KIM, JAE-SUNG,CHOI, DAE WOO,KIM, CHUN SUNG,YU, SUN-KYOUNG,KIM, HEUNG-JOONG,GO, DAE-SAN,LEE, SEUL AH,MOON, SUNG MIN,KIM, SU GWAN,CHUN, HONG SUNG,KIM, JEONGSUN,KIM, JONG-KEUN,KIM, DO KYUNG Potamitis Press 2018 Anticancer research Vol.38 No.6