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중합효소연쇄반응법을 이용한 급성 치수 및 치근단 질환의 병원성 세균의 동정
김지훈,유소영,임선아,국중기,임상수,박슬희,황호길 大韓齒科保存學會 2003 Restorative Dentistry & Endodontics Vol.28 No.2
The purpose of this study was to investigate the frequency of 7 putative pathogens in endodontic infections. The specimens were collected from infected pulpal tissue of patients who were referred for root canal treatment to the department of conservative dentisty, Chosun University. Samples were collected aseptically using a barbed broach and a paper point. The cut barbed broaches and paper points were transferred to an eppendorf tube containing 500 ml of 1 X PBS. DNAs were extracted from the samples by direct DNA extraction method using lysis buffer (0.5% EDTA, 1% Triton X-100). Identification of 7 putative pathogens was performed by PCR based on 16S rDNA. The target species were as follows: Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Bacteroides forsythus, Actinobacillus actinomycetemcomitans, and Treponema denticola. Our data revealed that the prevalence of P.endodontalis was found in 88.6% (39 / 54), P.gingivalis 52.3% (23 / 44), P.nigrescens 18.2%(8 / 44), P.intermedia 15.9% (7 / 44), B.forsythus 18.2% (8 / 44), A.actinomycetemcomitans 2.3% (1 / 44), T.denticola 25% (11 / 44) of the samples. The high prevalence of P. endodontalis and P.gingivalis suggests that they may play an important role in the etiology of endodontic infections.
Low-Dose Systemic Methotrexate Therapy for Recalcitrant Alopecia Areata
( Seul-ki Lim ),( Cho-ah Lim ),( In Sun Kwon ),( Myung Im ),( Young-joon Seo ),( Chang-deok Kim ),( Jeung-hoon Lee ),( Young Lee ) 대한피부과학회 2017 Annals of Dermatology Vol.29 No.3
Background: Alopecia areata (AA) is an autoimmune skin disease difficult to manage and treat. The pathogenesis of AA features a T-cell-associated autoimmune process, and systemic immunosuppressive therapy is prescribed widely for AA. Objective: To evaluate the efficacy and tolerance of systemic low-dose methotrexate (LD-MTX) therapy in treatment of recalcitrant AA multiplex. Methods: In a retrospective, non-controlled study, we evaluated 29 patients with recalcitrant AA treated with LD-MTX and assessed the therapeutic response according to severity of disease, disease duration, cumulative dose of MTX, and drug safety. Results: MTX was administered twice weekly, and the mean maximum weekly dose was 14.48 mg. The response was A5 (regrowth=100.0%) in 14 (48.3%) patients and A4 (regrowth of 75%∼90%) in 12 (41.4%) patients. Three patients had poor response to LD-MTX treatment (A2: n=2 [6.9%], A1: n=1 [3.4%]). All three of the patients showing a poor response had disease durations exceeding 24 months. Relapse was observed in 31% of patients with more than 75% regrowth. Common side-effects were elevated liver enzyme levels and gastrointestinal discomfort. Conclusion: LD-MTX appears to be an effective and well-tolerated treatment for recalcitrant AA multiplex. (Ann Dermatol 29(3) 263∼267, 2017)
P070 : Genkwadaphnin inhibits the growth of squamous cell carcinoma (SCC) cells
( Seul Ki Lim ),( Zheng Jun Li ),( Kyung Cheol Sohn ),( Myung Im ),( Young Lee ),( Chang Deok Kim ),( Young Joon Seo ),( Jae Wha Kim ),( Jeung Hoon Lee ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2
Background: Genkwadaphnin was isolated from the flower buds of Daphne genkwa, showing cytotoxicity against several cancer cell lines. Objectives: To investigate the effect of Genkwadaphnin on SCC cells. Methods: SCC12 cells were treated with Genkwadaphnin and effects were evaluated. Results: Genkwadaphnin treatment resulted in apoptosis of SCC12 cells in the dose- and time-dependent manner, evidenced by cleavage of procaspase-3 and PARP. Genkwadaphnin induced phosphorylations of JNK and p38 MAPK. When JNK and p38 MAPK were knockdowned by recombinant adenovirus expressing microRNA (miR), Genkwadaphnin-induced apoptosis was significantly inhibited. Pretreatment of SCC12 cells with the ROS scavenger N-acteylcysteine (NAC) inhibited Genkwadaphnin-induced apoptosis significantly. Conclusion: These results suggest that Genkwadaphnin has an anti-cancer potential against SCC cells.
Effects of Dietary from Safflower Bud on the Osteoporosis in Ovariectomized Rats
Seul Ki Lim,Dong Il Kim,Min Jung Park,Joo Hee Choi,Young Kuk Kim,An Chul Lee,Mi Young Choi,Soo Hyun Park 대한의생명과학회 2014 Biomedical Science Letters Vol.20 No.3
It has been reported that safflower seeds have preventive effects against osteoporosis. Recently, safflower buds (SB) were found to have more useful functional ingredients than safflower seeds. In the current study, we evaluated the anti-osteoporosis effects of SB diet in ovariectomized (OVX) rats. The rats were divided into five groups; sham operated group, OVX alone group, OVX plus 17β-estradiol (E2 10 μg/kg, i.p.) and OVX plus SB diet feeding group (0.3% or 1%). Feeding of SB diet (0.3% or 1%) to OVX rats markedly increased bone mineral density (BMD) of femurs, compared to the OVX group. The OVX rats exhibited a marked increase in trabecular separation (Tb.Sp) and this change was inhibited by the feeding of SB diet, similar to that seen with OVX+E2 group. Moreover, feeding of SB diet to OVX rats decreased the markers of bone turnover, including osteocalcin and alkaline phosphatase (ALP). These results suggest that SB extract has a bone sparing effect in OVX-induced trabecular bone loss and prevents deterioration of bone microarchitecture by suppressing the rate of bone turnover. Therefore, SB may be useful for preserving bone mass and structure in estrogen deficient women with a potential role in reducing postmenopausal osteoporosis.
P076 : Putting the melanosomes into squamous cell carcinoma (SCC) cells
( Seul Ki Lim ),( Hye In Choi ),( Kyung Cheol Sohn ),( Myung Im ),( Young Lee ),( Chang Deok Kim ),( Young Joon Seo ),( Young Ho Lee ),( Jeung Hoon Lee ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2
Background: The incidence of squamous cell carcinoma (SCC) is different in ethnic groups or skin colors. Individuals with dark skin tone (African and/or Asian) have lower incidence of SCC compare to light skin tone (Caucasian). Objectives: To investigate the potential role of melanosomes in SCC. Methods: Melanosomes were isolated from MNT-1 cells, and introduced into SCC cells (SCC12). Results: Proliferation of SCC12 cells were markedly reduced when melanosomes were introduced into the cells. In addition, cell migration was also reduced in a dose-dependent manner. Introduction of melanosomes affected cell cycle of SCC cells, leading to G0/G1 cell cycle arrest. Furthermore, melanosomes led to decrease of Ras and phosphorylated Erk level in SCC12 cells. Conclusion: These results suggest that melanosomes have the potential to reduce SCC cell growth through the modulation of Ras-Erk signaling pathway.
High glucose stimulates the expression of erythropoietin in rat glomerular epithelial cells
Seul Ki Lim,Soo Hyun Park 한국실험동물학회 2011 Laboratory Animal Research Vol.27 No.3
It has been reported that the levels of erythropoietin are associated with diabetes mellitus. Glomerular epithelial cells, located in the renal cortex, play an important role in the regulation of kidney function and hyperglycemia-induced cell loss of glomerular epithelial cells is implicated in the onset of diabetic nephropathy. This study investigated the effect of high glucose on erythropoietin and erythropoietin receptor expression in rat glomerular epithelial cells. We found that 25 mM D-glucose, but not mannitol or L-glucose, stimulated erythropoietin mRNA and protein expression in a time dependent manner (>4 h) in rat glomerular epithelial cells. In addition, 25 mM glucose, but not mannitol or L-glucose, also increased the phosphorylation of erythropoietin receptor, suggesting a role for erythropoietin receptor phosphorylation in erythropoietin synthesis. We conclude that high glucose stimulates erythropoietin production and erythropoietin receptor phosphorylation in rat glomerular epithelial cells.