Protective Effects of Polysaccharide fraction Extracted from Turmeric Leaveson Intestinal Epithelial Barrier integrity

( Sera Kim ),( Mijin Oh ),( Ho-young Park ),( Sang-keun Ha ),( Yong-gon Park ),( Sang-hoon Lee ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.2

Intestinal epithelial barrier plays an important role to regulate para-cellular transport of water, nutrients and ions and to defend against toxic agents and pathogens as a first barrier. Therefore, maintaining barrier integrity is required to enhance entire gut health. Curcuma longa L. (Turmeric) is a well-known natural product with its outstanding antioxidant and anti-inflammatory properties. However, an aerial part of turmeric such as leaves has been wasted as a byproduct, even though it has a bioactive property. In order to prepare the turmeric leaf polysaccharide fraction (TPF), turmeric leaf water extract was dialyzed with 12~14 kD membrane (MW cut off 12,000) and precipitated with ethanol. A prebiotic activity score of TPF was measured with Lactobacillus plantarum and L.paracasi subsp. tolerans, and compared with that of control, fructooligosaccharides (FOS) and inulin. The score of TPF (1.003 ± 0.197, 0.620 ± 0.143) was higher than that of control (0.168 ± 0.026, 0.187 ± 0.120), and similar to that of FOS (1.138 ± 0.281, 0.938 ± 0.065) and inulin (0.658 ± 0.181, 0.628 ± 0.160). To investigate the effects of TPF on intestinal barrier integrity, the effect of TPF on tight junction was measured in TNF-α induced Caco-2 cells. The result shows that trans-epithelial electrical resistance of TPF treated group was maintained relatively constant until 72 hr at 70.38%, comparingwith that of control group as 28.07%. In addition, TPF significantly increased the expression of tight junction-related proteins such as ZO-1 and Occludin. Taken together, our study demonstrates the prebiotic activity and protective effect of a polysaccharidefraction of turmeric leaf on intestinal epithelial barrier functions.

Combined rTMS to the auditory cortex and prefrontal cortex for tinnitus control in patients with depression: a pilot study

Park, Sera,Park, Hae-Jung,Kyeong, Sung-Hyon,Moon, In Seok,Kim, Minbum,Kim, Hee Nam,Choi, Jae-Young Informa Healthcare 2013 Acta oto-laryngologica Vol.133 No.6

<P><I>Conclusion:</I> The study showed that combined repetitive transcranial magnetic stimulation (rTMS) on the auditory cortex and prefrontal cortex has more benefit than rTMS on the auditory cortex alone for tinnitus control in patients with depression. Further studies for the most optimal combination of stimulation on both areas are needed. <I>Objective:</I> Recent studies suggest that the neuronal network changes of chronic tinnitus are beyond the auditory pathway. There is increasing evidences for the application of rTMS on multiple brain cortices in addition to the auditory cortex for the treatment of tinnitus. Sequential rTMS was performed on the auditory cortex alone as well as the auditory cortex combined with prefrontal cortex in patients with both chronic tinnitus and depression. <I>Methods:</I> Patients who presented with chronic tinnitus of more than 1 year were enrolled in the present study (seven males, four females; mean age 54 years). To select the site for the rTMS, PET CT was performed. Patients received the first rTMS on the primary auditory cortex for 5 days and on the primary auditory cortex and prefrontal cortex in the second application after tinnitus relapse. The Tinnitus Handicap Inventory (THI), visual analog scale (VAS), and Beck Depression Inventory (BDI) were evaluated before and after rTMS. <I>Results:</I> The mean THI score of the eight patients with depression changed from 77.5 ± 15 to 61.8 ± 20.1 after the second rTMS. There was statistical significance only for the second rTMS. The VAS score changed from 8.6 ± 1.6 to 6.3 ± 1.8 after the first rTMS and from 7.6 ± 2.4 to 4.6 ± 2.7 after the second rTMS, showing statistically significant changes both times. The THI changes after the second rTMS were greater than after the first rTMS, and the changes in VAS score showed a similar pattern. The changes in BDI score, which indicates the severity of depression, showed a variable pattern after rTMS. Patients with mild depression (10≤ BDI score <16, <I>n</I> = 4) showed significant improvement of THI with the second combined rTMS (ΔTHI = 24.5) as compared with the first rTMS on the auditory area (ΔTHI = 6). In contrast, combined rTMS did not show any better improvement on THI (ΔTHI = 6.5) than the first rTMS on the auditory cortex (ΔTHI = 7) in patients without depression (BDI <10, <I>n</I> = 3) and patients with moderate to severe depression (BDI ≥16, <I>n</I> = 4).</P>

Anti-SARS-CoV-2 Neutralizing Antibody Responses after Two Doses of ChAdOx1 nCoV-19 vaccine (AZD1222) in Healthcare Workers

Lim Sera,Lee Yuil,Kim Dong Wan,Park Won Sang,Yoon Jung Hwan,Lee Jung Young 대한감염학회 2022 Infection and Chemotherapy Vol.54 No.1

Background The kinetics of neutralizing antibodies against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) play an important role in evaluating vaccine efficacy and durability, herd immunity, additional vaccination, and prediction models of immune protection against coronavirus disease 2019. Materials and Methods Serum collection times were 4 and 8 weeks after 1st inoculation of AZD1222 (AstraZeneca, Cambridge, UK), and 2 and 16 weeks after 2nd inoculation with 12-week dosing intervals. Neutralizing antibody (Nab) titers were measured indirectly using commercially available R-FIND SARS-CoV-2 Neutralizing Antibody ELISA Kit (SG Medical Inc., Seoul, Korea). Possible influences of gender, age, and adverse events on neutralizing antibody titer were also investigated. Results Nab titers (median inhibition %) started to decrease shortly after reaching peaks. This decrease was more pronounced in the elderly group (≥56 years) than in the young group (≤39 years) at 8 weeks (49.5% vs. 55.4%, P = 0.021) and 16 weeks (40.6% vs. 53.9%, P = 0.006) after the 1st and 2nd inoculation. And Nab titers were inversely correlated with age in the 8-week (r = -0.2091, P = 0.0284) and the 28-week group (r = -0.2811, P = 0.0029). Seropositive conversion of Nab reached 89.1% and 100% following 1st and 2nd inoculation. This 100% seropositivity was dropped sharply to 74.5% after 16 weeks. Compared to subjects without adverse events (51.8%), median inhibition was higher in subjects with one or more systemic adverse events (74.2%, P = 0.0203) or those with one or more local and systemic adverse events (77.1%, P = 0.0003). Conclusion Nab induced by AZD1222 (AstraZeneca, UK) vaccination started to degrade shortly after the production period. Nab titers were lower in the elderly than in younger group during the degradation period. This seems to be because the degradation process of Nab is more pronounced in the elderly. This may explain why the frequency of breakthrough infections, disease severity, and mortality were higher in the elderly and may require revaccination to ensure robust immunity. Background The kinetics of neutralizing antibodies against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) play an important role in evaluating vaccine efficacy and durability, herd immunity, additional vaccination, and prediction models of immune protection against coronavirus disease 2019. Materials and Methods Serum collection times were 4 and 8 weeks after 1st inoculation of AZD1222 (AstraZeneca, Cambridge, UK), and 2 and 16 weeks after 2nd inoculation with 12-week dosing intervals. Neutralizing antibody (Nab) titers were measured indirectly using commercially available R-FIND SARS-CoV-2 Neutralizing Antibody ELISA Kit (SG Medical Inc., Seoul, Korea). Possible influences of gender, age, and adverse events on neutralizing antibody titer were also investigated. Results Nab titers (median inhibition %) started to decrease shortly after reaching peaks. This decrease was more pronounced in the elderly group (≥56 years) than in the young group (≤39 years) at 8 weeks (49.5% vs. 55.4%, P = 0.021) and 16 weeks (40.6% vs. 53.9%, P = 0.006) after the 1st and 2nd inoculation. And Nab titers were inversely correlated with age in the 8-week (r = -0.2091, P = 0.0284) and the 28-week group (r = -0.2811, P = 0.0029). Seropositive conversion of Nab reached 89.1% and 100% following 1st and 2nd inoculation. This 100% seropositivity was dropped sharply to 74.5% after 16 weeks. Compared to subjects without adverse events (51.8%), median inhibition was higher in subjects with one or more systemic adverse events (74.2%, P = 0.0203) or those with one or more local and systemic adverse events (77.1%, P = 0.0003). Conclusion Nab induced by AZD1222 (AstraZeneca, UK) vaccination started to degrade shortly after the production period. Nab titers were lower in the elderly than in younger group during the degradation period. This seems to be because the degradation process of Nab is more pronounced in the elderly. This may explain why the frequency of breakthrough infections, disease severity, and mortality were higher in the elderly and may require revaccination to ensure robust immunity.

소아청소년에서의 항생제 사용량 변화에 따른 그람음성 균주의 항생제 내성률 변화 양상

박세라 ( Sera Park ),이은택 ( Euntaek Lee ),소혜진 ( Hye Jin So ),유리나 ( Ree Nar Yoo ),이진아 ( Jina Lee ) 대한소아감염학회 2021 Pediatric Infection and Vaccine Vol.28 No.2

목적: 본 연구에서는 소아환자의 혈액에서 분리된 주요 그람음성 균주의 항생제 내성 양상을 파악하고 항생제 사용량과의 관계를 알아보고자 하였다. 방법: 2014년 1월부터 2018년 6월까지 서울아산병원 어린이병원에 입원한 18세 이하의 소아청소년에서 발생한 그람음성 균주 균혈증 중 Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, 총 4가지 균주를 대상으로 항생제 내성 양상을 분석하였다. 또 원내에서 소아에게 주로 사용하는 정주용 항생제의 사용량과 항생제 내성률의 변화와의 관련성을 분석하였다. 결과: 본 연구 기간 동안 전체 혈액배양에서, K. pneumoniae (6.4%; 105/1,628), E. coli (5.6%; 91/1,628), P. aeruginosa (3.3%; 54/1,628) 및 A. baumannii (2.5%; 41/1,628)의 순으로 분리되었다. 2014-2018년에 분리된 E. coli와 K. pneumoniae의 광범위 cephalosporin 계열에 대한 내성률은 각각 49.3%와 54.4%였다. 2014년도에는 carbapenem에 내성을 보이는 E. coli 균종이 처음 출현하였고, 5년간의 내성률은 5.3%였다. 연구기간 동안 항생제 사용량과 항생제 내성률 사이의 상관관계를 분석하여, 3세대 cephalosporin 사용량과 A. baumannii의 내성률(r²=0.96, P=0.004) 그리고 carbapenem의 사용량과 K. pneumoniae의 내성률 (r²=0.79, P=0.045) 간에 양의 상관관계를 확인하였다. 결론: 국내 소아에서 발생한 그람음성균 균혈증 분석 시 3세대 및 4세대 cephalosporin에 대한 높은 내성률이 확인되며, carbapenem에 대한 내성도 확인되고 있다. 일부 항생제 사용량과 해당 약제에 대한 내성률에 선형 상관관계가 있었으며, 이는 항생제 사용량이 내성률에 영향을 미칠 수 있음을 시사한다. 이에 지속적인 원내 항생제 사용량 및 내성률에 대한 감시가 필요하며, 적절한 항생제의 선택 및 관리는 내성균주 출현 예방 및 치료 성적 향상에 필수적이다. Background: The purpose of this study was to investigate the association between antibiotic use and the antimicrobial resistance of gram-negative bacteria isolated from blood cultures in a pediatric population. Methods: From January 2014 to June 2018, the antibiotic resistance pattern of Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa obtained from bacteremic patients aged ≤18 years hospitalized at Asan Medical Center Children's Hospital was analyzed and the parenteral antibiotic consumption data were retrieved. Results: During the study period, the blood culture was positive for K. pneumoniae (6.4%; 105/1,628), E. coli (5.6%; 91/1,628), P. aeruginosa (3.3%; 54/1,628), and A. baumannii (2.5%; 41/1,628), and the extended-spectrum antibiotic resistance rate of gram-negative bacteria was consistently high. The overall resistance rate of E. coli and K. pneumoniae to extended- spectrum cephalosporin was 49.3% and 54.4%, respectively. Carbapenem-resistant E. coli was first detected in 2014; its overall resistance rate to carbapenem was 5.3%. There was a linear correlation between the usage of 3rd generation cephalosporin and the resistance of A. baumannii (r²=0.96, P=0.004) and carbapenem usage and the resistance of K. pneumoniae (r²=0.79, P=0.045). Conclusions: A positive linear correlation was observed between antibiotic resistance and the corresponding antibiotic usage in 3rd generation cephalosporin resistant A. baumannii and carbapenem resistant K. pneumoniae. The judicious use of antibiotics in healthcare settings is important to minimize selection for extended-spectrum β-lactamase (ESBL) and carbapenem resistance in gram-negative bacteria.

표면 플라스몬 공명을 이용한 TP53의 점돌연변이 검출

박세라(Sera Park),장대호(Daeho Jang),신세현(Sehyun Shin) 대한기계학회 2015 대한기계학회 춘추학술대회 Vol.2015 No.11

TP53, a type of tumor suppressor gene, regulates other mutations involving numerous cancers which mean that its mutation causes fatal disease to human. Thus, sensitive detection technique of this gene is necessary for early diagnosis and targeted therapy. In this study, we developed the detection of TP53 of one-point mutation using surface plasmon resonance (SPR). We immobilized the complementary DNA of wild type TP53 as a receptor on bare gold chip surface which is attached thiol functional group (Probe; HS-C6-26mer). Wild type DNA and one-point mutated DNA are injected into two different micro channels formed on a single gold sensor chip simultaneously. Because the affinity between receptor DNA and wild type DNA is greater than it of receptor and mutation DNA, the SPR signal from wild type DNA channel was three-times higher than one-point mutated DNA. As a result, our sensor is able to distinguish remarkably point mutation with wild type of the gene. Accordingly, this technique will be a powerful biosensor for detection of the mutation and helpful to preventing metastasis of cancer.

PSS/E HVDC Generic 모델을 위한 OLRPC 개발

박세라(Sera Park),유현재(Hyunjae Yoo),이경빈(Kyungbin Lee),성판영(Panyoung Sung) 대한전기학회 2019 대한전기학회 학술대회 논문집 Vol.2019 No.7

국내 뱀류 9종의 비늘 크기와 형태 비교

구교성 ( Koo Kyo Soung ),박소현 ( So Hyun Park ),김종선 ( Jong Sun Kim ),권세라 ( Sera Kwon ),최우진 ( Woo Jin Choi ),박일국 ( Il Kook Park ),조한나 ( Han Na Cho ),박재진 ( Jae Jin Park ),오홍식 ( Hong Shik Oh ),박대식 ( Daesik Pa 한국하천호수학회(구 한국육수학회) 2017 생태와 환경 Vol.50 No.2

Body scales directly exposed to external environments can be an important factor to understand various characteristics of a species such as habitat features, life history and basic ecology. In this study, we compared size and morphology of dorsal, outermost dorsal, keeled dorsal and ventral scales of total nine snake species in Korea; Oocatochus rufodorsatus, Elaphe dione, Rhabdophis tigrinus, Amphiesma vibakari, Dinodon rufozonatum, Hierophis spinalis in the Colubridae and Gloydius ussuriensis, G. brevicaudus, G. saxatilis in the Viperidae. The morphological characteristics of the scales seem to well reflect foraging modes and moving activity of both families. Uniquely D. rufozonatum had a diamond shape dorsal scale and had the greatest and smallest value of the ratio of width/length of dorsal and ventral scales, respectively. O. rufodorsatus, D. rufozonatum and H. spinalis did not have keeled dorsal scales and E. dione had keel on the few of dorsal scales. In addition, morphological characteristics of scales of three viper species were closely consistent with previously known phylogenetic relationships.

Optimization of Extraction Conditions for Antioxidant Components Containing Curcuma longa (Turmeric) Leaves using Response Surface Methodology

Sera Kim,Seok-Chun Ko,Yoon-Sook Kim,Sang-Keun Ha,Ho-Young Park,Sang-Hoon Lee 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

Improvement of device performance and instability of tungsten-doped InZnO thin-film transistor with respect to doping concentration

Park, Hyun-Woo,Song, AeRan,Kwon, Sera,Du Ahn, Byung,Chung, Kwun-Bum JAPAN SOCIETY OF APPLIED PHYSICS 2016 Applied physics express Vol.9 No.11

<P>W-doped InZnO (WIZO) thin-film transistors (TFTs) were fabricated by co-sputtering with different W doping concentrations. We varied the W doping concentration to change the device performance and stability of the WIZO TFTs. WIZO TFTs with a W doping concentration of similar to 1.1% showed the lowest threshold voltage shift and hysteresis. We correlated the device characteristics with the evolution of the electronic structure, such as band alignment, chemical bonding states, and band edge states. As the W doping concentration increased, the oxygen-deficient bonding states and W suboxidation states decreased, while the conduction-band offset and the incorporation of the WOx electronic structure into the conduction band increased. (C) 2016 The Japan Society of Applied Physics</P>

Extreme precipitation changes over South Korea Projected by the multi-RCM under RCP Scenarios

Sera Jo,Joong-Bae Ahn,Myoung-Seok Suh,Dong-Hyun Cha,Dong-Kyou Lee,Song-You Hong,Seung-Ki Min,Seong-Chan Park,Hyun-Suk Kang,Kyo-Moon Shim 한국기상학회 2016 한국기상학회 학술대회 논문집 Vol.2016 No.10