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- 저자 : ( Seonkwan Kim ) (검색결과 4 건)
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KIM, SEONKWAN,PARK, SUNHYANG,KIM, YOUNGHO UNKNOWN 2014 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.34 No.3
<P>The lysyl oxidase (LOX) family is an emerging family of amine oxidases that is responsible for lysine-mediated crosslinks found in collagen and elastin. Several novel functions, such as tumor suppression, tumor progression, cellular senescence, chemotaxis and the modification of histones have recently been attributed to the LOX family of proteins. In the search for more human LOX paralogs, in the present study, we identified several expressed sequence tag (EST) clones that showed an alternative exon-intron splice pattern from LOX. These ESTs corresponded to the LOX transcript variant?2 (LOX-v2) that was recently reported in GenBank (accession?no.?NM_001178102). LOX-v2 mRNA lacks exon?1 of LOX, but contains an additional 222?bp sequence from the 5'-flanking intronic region of exon?2. The deduced LOX-v2 polypeptide contains the characteristic C-terminal domains of the LOX family, but does not contain the N-terminal propeptide region that has been reported to have tumor suppressor activity. In peroxidase-coupled fluorometric assays, LOX-v2 showed β-aminopropionitrile-inhibitable amine oxidase activity toward collagen and elastin. RT-PCR analysis of human tissues revealed a distinct tissue specificity of LOX-v2 expression compared to that of LOX. Promoter assays indicated that an alternative promoter element present in the exon?1 region of LOX was sufficient for the differential expression of LOX-v2. These findings indicate that the human LOX gene encodes 2?variants, LOX and LOX-v2, both of which function as amine oxidases with distinct tissue specificities.</P>
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Variations in <i>LOXL1</i> associated with exfoliation glaucoma do not affect amine oxidase activity
Kim, Seonkwan,Kim, Youngho Molecular Vision 2012 Molecular vision Vol.18 No.-
<P><B>Purpose</B></P><P>Lysyl oxidase-like 1 (LOXL1) is a copper-dependant amine oxidase that plays an essential role in elastogenesis. Two non-synonymous single-nucleotide polymorphisms of <I>LOXL1</I>, R141L (rs1048661) and G153D (rs3825942), have been reported to significantly increase susceptibility to exfoliation glaucoma (XFG). To evaluate the impact of the R141L and G153D variations on the amine oxidase activity of LOXL1, we generated four different haplotypes of LOXL1 with R141L and G153D and assessed the amine oxidase activity of the LOXL1 variant proteins.</P><P><B>Methods</B></P><P>The four different haplotype variants of LOXL1 were created by oligonucleotide-directed mutagenesis in an LOXL1 expression vector. Recombinant LOXL1 variant proteins were purified by nickel-affinity chromatography. The amine oxidase activities of the LOXL1 variant proteins were assessed using peroxidase-coupled fluorometric assays.</P><P><B>Results</B></P><P>All of the haplotype variants of LOXL1 (141R-153G, 141R-153D, 141L-153G, and 141L-153D) showed β-aminopropionitrile-inhibitable amine oxidase activity toward elastin, type I collagen, and cadaverine, indicating that each LOXL1 variant functions as an amine oxidase. However, there were no significant differences in amine oxidase activity between the LOXL1 haplotype variants toward the tested substrates.</P><P><B>Conclusions</B></P><P>The R141L and G153D variations in the NH<SUB>2</SUB>-terminal region of LOXL1 do not affect the amine oxidase activity of LOXL1. This is consistent with recent genetic findings on the reversal of risk alleles of R141L and G153D in different ethnic backgrounds. Our results suggest that other unknown genetic factors or molecular mechanisms may be more relevant to the development of XFG.</P>
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The Effectiveness of Visualization System for Virtual Reality Learning
Soohwan Kim,Kil Hong Joo,Seonkwan Han,JinTak Choi 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.12
In this study, we developed a visualization tool that shows learners’ interactive activities graphically during virtual reality (VR) education. Current VR system only shows learning content. However, we need to recognize learners’ interaction and information exchanges for increasing effectiveness of learning; also we need to show it effectively. Therefore, we developed visualization tool for VR learning based on web 2.0, and this tool helps teachers and students to monitor and percept the interaction activities among students, and thus it facilitates their interaction and learning in VR education environment. For experiments, 68 students participated in VR learning. The result shows that the interaction and the perception of effectiveness of learning are significant differences between VR system and web 2.0 VR system. Furthermore, for verify availability of usage, TAM (Technology acceptance model) analysis about the system is supported. This result will be basic material for constructing of future VR learning environment.
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Confirmation of genotypic effects for the bovine APM1 gene on marbling in Hanwoo cattle
( Anam Kwon ),( Krishnamoorthy Srikanth ),( Eunjin Lee ),( Seonkwan Kim ),( Hoyoung Chung ) 한국동물자원과학회(구 한국축산학회) 2016 한국축산학회지 Vol.58 No.4
Background: Our previous study had identified the SNP (g.81966377T > C) and indel (g.81966364D > I) located in the promoter of APM1 to have a significant effect on marbling in Hanwoo. APM1 encodes an adipocytokine called adiponectin, which plays a significant role in lipogenesis. The aim of this study was to verify and validate the effect of the SNP and indel on marbling and other carcass traits in a large, representative, countrywide population of Hanwoo cattle. The carcass traits measured were marbling (MAR), backfat thickness (BFT), loin eye area (LEA), and carcass weight (CAW). Results: Primers were designed to amplify 346 bp of the genomic segment that contained the targeted SNP (g.81966377) and the indel (g.81966364). After data curation, the genotypes of 8,378 individuals identified using direct sequencing analysis estimated frequencies for C (0.686) and T (0.314) respectively showing genotype frequencies for CC (0.470), CT (0.430) and TT (0.098). The genotypes were significantly associated with MAR, BFT and LEA. The indel had significant effect on marbling (P < .0001) with strong additive genetic effects. The allele frequencies was estimated at (DEL, 0.864) and insertion (INS, 0.136) presenting genotypes of D/D (75.63 %), D/I (21.44 %), and I/I (2.92 %). Significant departure from Hardy-Weinberg equilibrium was not detected for both the SNP and the indel. Conclusion: The SNP genotypes showed significant association with MAR, BFT and LEA with strong additive genetic effects, while the indel was significantly associated with MAR. The results confirmed that the variants can be used as a genetic marker for improving marbling in Hanwoo.
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