Changes in cAMP Response and Testosterone to 17β-Estradiol to FSH in Isolated Rat Sertoli Cells-Age and Dose Related Differences

Cho, Sung Hoon,Lee, Byung Churl,Seong, Sang Min CATHOLIC MEDICAL CENTER 1986 Bulletin of the Clinical Research Institute Vol.14 No.1

The Seroli cell was identified as> the main primary target site for the action of follicle stimulating hormone (FSH) in the testis. FSH has been known to bind specifically to Sertoli cell and to evoke a series of biochemical responses such as increased accumulation of cyclic AMP, production of androgen binding protein, and steroid metabolism. Recently age related changes in FSH stimulation of cAMP and aromatization of testosterone of 17β-estradiol were demonstrated. The purpose of this study was to investigate the changes in cAMP and testosterone to 17β-estradiol aromatization responses of FSH with dose differences in isolated rat Sertoli cells of different age. Methods for preparation of rat Sertoli cells were adapted from those used to prepare rat Sertoli cells. Homogenous preparations of primary Sertoli cell cultures were obtained from the testis of the rat of different age: prepubertal (14 days old rat), pubertal (18 days old rat) and adult (60 days old rat). For cAMP determinations, cells were stimulated for 30 min at 37?C with different dose of FSH in media containing 0.1 mg/m]l l-methyl-3-isobutylxanthine. cAMP assays were performed in duplicate using radioimmunoassay kit. FSH stimulated aromatization of testosterone of 17j3-estradiol was determined in cells stimulated with different dose of FSH in media containing 0.5 μmol/ml testosterone. 170-estradiol assays were performed in duplicate by means of specific radioimmunoassay. The results were as follows: 1. Sertoli cells from adult rats responded to FSH stimulation with over 30-fold rise at 5 min. At 30 min, cAMP is significantly lower and down to baseline level by 60 min. 2. There were age-related differences in Sertoli cell cAMP response after 30 min of FSH stimulation. Sertoli cells from pubertal rats responded most sensitively to FSH with a dose response relationship. 3. The heighest aromatase enzyme activity in converting testosterone to 17(3-estradiol was found in pubertal rat Sertoli cells. Sertoli cells from pubertal rats demonstrated a dose-response of FSH stimulation of testosterone to estradiol conversion. In conclusion, these results demonstrate that the age and dose-related differences in rat Sertoli cell response to FSH, and also provide direct evidence that the Sertoli cells represent a primary target site for FSH activity in the testes.

응급실에서 기관내 삽관후 발생한 후두육아종 1례

조남수,김용배,조수형,송민엽,송성용 大韓應急醫學會 1996 대한응급의학회지 Vol.7 No.1

Endotracheal intubation has been used widely for the maintenance of airway in the emergency patient and CPR in the acute airway obstruction patient and to treat respiratory insufficiency. Endotracheal intubation has many advantages but it may cause several complications which rate about 4% of endotracheal intubation patient. Among these complications, laryngeal granuloma is a relatively rare complication arising about 1 case per 10,000∼20,000 cases. Authors report a case of laryngeal granuloma which developed after endotracheal intubation required during emergency treatment. Case: A 55-year old female patient visited Chosun University Hospital Emergency Room complaining of dyspnea, cyanosis and sweating on Feb. 8th 1995. According to past history, she had a little obesity, dyspnea on exercise and mild hypertension. At arrival, blood pressure was 240/110mmHg, pulse rate 150 rates/min, respiratory rate 12/min, body temperature 36.5℃ and cyanosis with sweating on whole body was observed. And the mental state was semicoma. At 5 minutes after arrival, sudden respiratory arrest developed and immediate endotracheal intubation which emergency treatment was performed it was difficult to intubate endotracheally due to short neck and obesity. At 1 hour after arrival, general condition of the patient recovered well and extubation was done. The patient was transferred to internal medicine department and discharged after 12 days of medical treatment. At 2 months after discharge, the patient felt a progressive hoarseness and foreign body sensation of larynx. She was diagnosed as laryngeal granuloma at ENT department of Chosun University. She underwent the surgical operation and discharged in good health after 7 days of admission.

Purification and Characterization of Helicobacter pylori ${\gamma}$-Glutamyltranspeptidase

Song, Jae-Young,Choi, Yeo-Jeong,Kim, Jeong-Min,Kim, Yoo-Ree,Jo, Jin-Seong,Park, Jin-Sik,Park, Hee-Jin,Song, Yun-Gyu,Lee, Kon-Ho,Kang, Hyung-Lyun,Baik, Seung-Chul,Youn, Hee-Shang,Cho, Myung-Je,Rhee, Kw The Korean Society for Microbiology 2011 Journal of Bacteriology and Virology Vol.41 No.4

Gamma-glutamyltranspeptidase (GGT) was purified to electrophoretic homogeneity from the cell extract of H. pylori. The purified enzyme consisted of heavy and light subunits with molecular weights of 38 kDa and 21 kDa, respectively. N-terminal amino acid sequence of heavy and light subunits revealed that H. pylori GGT was processed into 3 parts for a signal peptide of 27 amino acid residues, a heavy subunit of 352 residues, and a light subunit of 188 residues during translation. The reaction rate for hydrolysis of ${\gamma}$-GpNA was 84.4 ${\mu}mol/min$ per milligram of protein, and that for the ${\gamma}$-glutamyl transfer from ${\gamma}$-GpNA to gly-gly was 23.8 ${\mu}mol/min$ per milligram of protein. The apparent Km values of H. pylori GGT for ${\gamma}$-glutamyl compounds were on the order of $10^{-3}$ to $10^{-4}$ M and those for acceptor peptides and amino acids were on the order of $10^{-1}$ to $10^{-2}$ M. The GGT protein kept approximately 80% of the initial enzymatic activity on incubation at $60^{\circ}C$ for 15 min. The optimum temperature and pH for reactions of both hydrolysis and transpeptidation were $40^{\circ}C$ and 9.0, respectively. The transpeptidation and hydrolysis reactions catalyzed by H. pylori GGT were strongly inhibited by L-Gln and moderately inhibited by L-Ala, L-Ser, ${\beta}$-chloro-L-Ala, and L-Glu. These results demonstrated that the biochemical properties of H. pylori GGT are different from those of other bacterial GGTs. Further, H. pylori GGT might degrade glutathione in the gastric mucous layer of humans if the enzyme could be secreted in the bacterial niches.

일 장기 수용시설 노인들의 인지기능장애와 우울증상의 유병율

조맹제,함봉진,이부영,김성윤,신영민,이창인 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.5

목 적 : 본 연구는 수용시설의 노인들에서 인지기능장애와 우울증상의 유병율을 조사하기 위한 것이다. 방 법 : 한국의 한 수용시설인 꽃동네에 거주하는 60세 이상 노인 169명을 대상으로 한국판 Mini-Mental State Examination(MMSE-K). Short Portable Mental State Questionnaire(SPMSQ), 축약판 Geriatric Depression Scale(SGDS). 그리고 the Center for Epidemiologic Studies Depression (CES-D)을 적용하였다. 결 과 : 전체 169명중 153명(남자=72명, 여자=79명, 그리고 누락=2명)이 검사를 완성하였다. 연령의 범위는 60에서 102세였고, 평균연령은 74.17±8.27(남자71.76±8.14. 여자76.14±7.78)세였다. 교육기간의 범위는 무학에서 16년이었고, 평균교육기간은 3.04±3.93(남자 4.13±4.23, 여자 2.01±3.38)년이었다. 인지기능장애의 유병율은 MMSE-K≤23의 기준으로 56.2%, SPMSQ≤6의 기준으로 26.1% 로 평가되었다. 비교적 분명한 인지기능장애의 기준으로 MMSE-K≤19를 적용하였을 경우 유병율은 25.5%로 이는 SPMSQ≤6을 기준으로 하여 얻은 결과와 유사한 수준이었다. 우울증상의 유병율은 SGDS≥10의 기준으로 19%, CES-D≥25의 기준으로 23.5%로 평가되었다. 비교적 가벼운 우울증상까지 선별하기 위하여 SGDS≥8을 기준으로 할 경우 유병율은 39.2%로 이는 CES-D≥20을 기준으로 하여 얻은 결과와 일치하는 수준이었다. MMSE-K와 SPMSQ, SGDS와 CES-D, MMES-K와CES-D, MMES-K와 SGDS, 그리 고·SPMSQ와 SGDS 사이에 의미있는 상관관계가 관찰되었다. 결 론 : 수용시설 노인들에서 인지기능장애와 우울증상의 유병율은 매우 높은 수준이며, 이러한 결과는 수용시설 노인들에 대한 보다 집중적의 의료서비스가 필요함을 시사한다. Objectives : This study was to investigate the prevalence of cognitive impairment and depressive symptomatology among the long-term institutionalized elderly people. Methods : Mini-Mental State Examination-Korean version(MMSE-K), Short Portable Mental State Questionnaire(SPMSQ), Short version of Geriatric Depression Scale(SGDS), and the Center for Epidemiologic Studies Depression(CES-D) were administerd to 169 elderly residents in an institution, 'Got Dong Rae'. Results : One hundred fifty-three(males=72, females=79, and missing=2) completed the entire examination. Age ranged from 60 to 102 years and the mean of age was 74.17±8.27(males 71.76±8.14, females 76.14±7.78) years. Duration of education ranged from 0 to 16 years and the mean of duration of education was 3.04±3.93(males 4.13±4.23, females 2.01±3.38) years. The prevalence of cognitive impairment was estimated as 56.2% by MMSE-K≤23 and 26.1% by SPMSQ≤6. The prevalence of relatively definite cognitive impairment defined by MMSE-K≤19 was 25.5%, which was similar to the result achieved by using SPMSQ≤6. The prevalence of depressive symptomatology was estimated as 19% by SGDS≥10 and 23.5% by CES-D≥25. In the case of using SGDS≥8 as cutoff-point to screen mild as well as severe depressive symptomatology, the prevalence was 39.2%. It was identical to the result achieved by using CES-D≥20. Significant correlations were observed between MMSE-K and SPMSQ, between SGDS and CES-D, between MMSE-K and CES-D, between MMSE-K and SGDS, and between SPMSQ and SGDS. Conclusion : The prevalencies of cognitive impairment and depressive symptomatology were high in institutionalized elderly people. This results suggests that more intensive mental health care is needed for long-term institutionalized elderly people.

솔잎(Pinus densifloras Z.)의 조성분이 전통 약주 발효에 미치는 영향

조남지,조민자,조규성 안성산업대학교 1995 論文集 Vol.27 No.-

藥酒 製造시 솔잎 添加 效果를 調査하기 위하여 솔잎 抽出物 첨가가 S. cerevisiae의 生育에 미치는 影響과, 솔잎분말을 添加한 제국 과정 중의 酵素 活性 및 약주 발효 과정 중 pH, 總酸, 알코올의 含量 變化를 調査하였다. 솔잎의 일반 성분으로는 수분 54.80%, 단백질 8.18%, 조지방 2.07%, 섬유 23.13%, 조회분 1.10%였다. 솔잎 첨가구가 대조구에 비하여 S.cerevisiae의 증식을 억제하는 효과가 있었다. 제국 과정 중의 amylase 활성은 모든 시험구에서 제국 24시간에서 최고의 활성을 나타내었고, 솔잎 2.0%구는 대조구에 비하여 높은 활성을 보였다. 산성 protease 활성은 솔잎 첨가구가 대조구에 비하여 효소활성이 높았으며, 솔잎 분말 2.0% 첨가구는 발효 24시간에서 활성이 가장 높았다. pH는 솔잎의 첨가량이 증가할수록 높았으나 발효기간이 경과할수록 감소하였다. 총산의 변화는 발효기간이 길어질수록 높았고, 0.1, 0.5, 1.0% 솔잎첨가구는 대조구에 비하여 총산의 함량이 높았으며 첨가량이 많아질수록 낮아졌다. 솔잎 분말의 첨가량이 증가할수록 알코올의 함량은 대조구보다 낮았으나, 첨가량에 따른 큰 영향은 나타나지 않았다. The study is to investigate the effects of addition of pine needles's extract on the growth of Saccharomyces cerevisiae and to investigate the changes of α-amylase activity, acid-protease activity, pH, total acidity, alcohol content during alcohol fermentation with variation of the amount of pine needles powder. The composition of pine needles sample were as follows : moisture 54.8%, crude protein 8.18%, crude lipid 2.07%, crude fiber 23.13%, crude ash 1.10%. Treatment groups with pine needles extract have an inhibitory effect on the growth of S. cerevisiae companre to control group. The highest activity of α-amylase was shown at 24 hours fermentation in all treatments and the only treated group with 2.0% addition of pine needles powder had higher amylase activity than that of control group. The activity of acid-protease in the treatments was higher than that of controls and the highest protease activity of 2.0% treated group was shown at 24 hours fermentation. The pH was increased in the adding more pine needles powder but it would be decreased with the lapse of fermentation time. The change of total acidity was increased with the longer fermentation. Although the content of total acidity was higher in 0.1, 0.5 and 1.0% treated groups than that of control group, the small in addition of pine needles powder the less total acid content. Although the alcohol content of treated groups were a little lower than that of control group, there were no different effect on the alcohol content as the additive amount of pine needles powder.

시험불안과 우울, 특성불안 및 상태불안과의 상호관계에 관한 연구

조수철,정영,홍강의,신민섭,성영훈 대한소아청소년정신의학회 2001 소아청소년정신의학 Vol.12 No.2

시험불안은 전세계적으로 학령기 아동 또는 청소년들이 공통적으로 경험하는 불안이라고 할 수 있다. 경우에 따라서는 어느 정도의 시험불안은 학습을 촉진시키기도 하지만 대부분의 경우에 학습을 저해시키는 요인으로 작용한다. 지난 수십년동안 시험불안의 요인이나 특성에 대하여 많은 연구들이 있어왔으나 단편적이며, 시험불안의 정확한 특성이나 원인에 대하여 아직 밝혀져 있지 않은 상태이다. 이에 본 연구에서는 시험불안의 특성을 규명하기 위하여 시험불안과 상태-특성불안, 우울증과의 상호관련성에 관하여 알아보았다. 서울시내 한 고등학교 학생들을 대상으로 하여 시험불안, 우울증, 상태-특성불안 간의 상호관계에 대하여 신뢰도와 타당도가 입증된 평가도구를 시용하여 상관성을 검증하였다. 시험불안과 우울증과의 상관성은 상관계수 0.56(p<0.05), 특성불안과의 상관성은 상관계수 0.53(p<0.05), 상태불안과의 상관성은 상관계수 0.75(p<0.05)로서 모두 의미있는 상관관계를 보여주었다. 이상의 결과로 미루어 시험불안은 단순한 시험불안이 아니라 우울증, 상태불안, 또는 특성불안과 밀접한 관계가 있다고 할 수 있다. 따라서 시험불안에 대한 치료적인 대책을 수립할 때에는 이러한 요인들이 고려되어야 한다. Introduction : Test anxiety is a pervasive problem among high school students in Korea. While anxiety in test situations may actually facilitate the performance of some students, more often it is disruptive and leads to performance decrements. Over the past years, many child psychiatrists have become concerned with understanding the nature of test anxiety, but it is not clearly understood yet. In order to understand the nature of test anxiety, the relationship between test anxiety and depression, state anxiety, trait anxiety was examined. In addition, the relationships between the subscores of test anxiety (worry and emotionality) and the subscores of CDI, state anxiety or trait anxiety were examined. Methods : The Test Anxiety Inventory, Chidlren's Depression Inventory, State-Trait Anxiety Inventory were administered to 425 high school students in Seoul. The relationships between test anxiety and other measures were tested using Pearson correlation coefficients and to test the causal ralationship among the variables, regression analysis was performed. Results : The correlation coefficients between test anxiety and depression, state anxiety, trait anxiety were 0.56(p<0.05), 0.75(p<0.05), 0.53(p<0.05) respectively. The correlation coefficients between subscales of test anxiety and depression were all significant. The correlation between subscales of test anxiety and state and trait anxiety were also statistically significant. Conclusions : This study indicates that test anxiety is closely related with depression, state and trait anxiety. In addition, the subscales of test anxiety are significantly related with those of the depression. The correlation coefficients between test anxiety and state-trait anxiety are also statistically significant. Thus, in order to develop the preventive and effective methods for treatment, these psychopathological characteristics should be kept in mind.

생체분해성 고분자를 이용한 사시수술용 제형개발 및 효용성에 대한 연구

민병무,김용백,김승영,김창식,박근성,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

A new device, muscle clamping system was developed to facilitate exact quatifying technique A strabismus surgery, and reduce the risk of complications. The device is composed of a lower fixing body with three jaws and an upper supporting body. They are used to clip an extraocular muscle and fix it to the sclera with a single bite. Superior rectus recession on 16 rabbit eyes were performed with this new device. Conjunctival injection, muscle adhesion strength, and light microscopic findings were examined at 1, 2, 4 and 8 weeks postoperatively. The Conjunctival injection were minimal, adhesion power ranged from 420 to 600 gram gravity, which is sufficient in withstand the normal pull of human extraocular muscle. In microscopic exam, some Inflammatory cells and fibrosis were found. The new device was technically easy, fast, and accurate, so it may be useful in stabismus surgery.

디클로로메탄 폭로 광학렌즈 제조업 근로자들의 혈액성상 및 자각증상

조영채,이태용,이동배,박성민 忠南大學校 環境問題硏究所 1997 環境硏究 Vol.15 No.-

This study was performed to find out the changes of hematological values, enzyme activities, and complain rates of subjective symptoms according to the Dichloromethane(DCM) exposure in optical lens manufacturing workers. The DCM exposed workers were divided into 3 groups that were consisted of the washing, setting and seperating group, and analyzed the DCM levels, hematological values(WBC, RBC, Hb, Hct), enzyme activities (s-GOT, s-GPT, TG and TC), and surveyed the subjective symptoms from 42 DCM exposed workers who worked in Taejon city and Chungnam provincial areas from Feb.1 to 18, 1996. The results were as follows: The mean values of DCM were significantly different from working process, that was 19.62±6.29ppm in washing, 25.08±8.27ppm in setting, 43.46±18.65ppm in seperating process. And the DCM exposed levels showed a tendency to increase when job tenure was longer and ventilation was worse. The values of WBC, RBC, s-GOT and TG were significantly increased according to the increase of DCM exposed levels. The complain rates of subjective symptoms of DCM exposed workers were high rates such as "heaviness", "sleepiness","nausea and vomiting". "decrease eye-sight", "hand tremor" and "dyspnea", respectively. The correlation between DCM exposed levels, and WBC, RBC and s-GOT were significantly different, respectively.

생체분해성 안과제형의 안구내흡수정도와 안독성

민병무,김용백,박근성,송규상,양준묵,이성복,조항진,길숙종,김승영,김학용 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

An improved retinal tack can be used to fix the retina in complicated retinal detachment and proliferative vitreoretinopathy. We used the retinal tack made by biodegradable polyglycolic acid, polylactic acid and copolymers. The tacks with polyglycolic acid were placed in the retina the right eyes of 10 rabbits as group 1, with polylactic-polyglycolic acid copolymers as group 2 in each left eyes. We examined the absorption rates and degree of intraocular inflammation at 1 day, 1, 2, 4, and 8 weeks and compared both eyes. The tacks were absorbed with time in both eyes, but more rapid in copolymers. And there was no inflammation in both eyes. Now, this study will be a base on the drug delivery system in intraocular pharmacological and surgical management.

수학 교수-학습에서의 구성주의적 관점

조성민,노선숙 이화여자대학교 교육과학연구소 1999 교육과학연구 Vol.30 No.-

If constructivism is to be accepted as a fundamental framework for today's information oriented open education, it must offer something more concrete than just "student construct his/her knowledge". In order to clarify the role of constructivism in teaching and learning of mathematics, detailed review of research trend on constructivism widely spread in mathematics education was carried out. The study was conducted focusing on describing the role of the teacher. The study showed that constructivism in mathematics education can be described as a policy of non-interference by the teacher. The teacher needs to have the proper insight into mathematics education to know and recognize the process of student's construction to help the process of learning.