초파리 자연집단의 유해유전자 빈도의 변화에 관한 연구

김승곤,추종길,김태전 서울保健大學 1987 論文集 Vol.7 No.1

Penicillinase Producing N.gonorrhoeae株와 Nom-Penicillinase Producing N.gonorrhoeae株의 약제 감수성에 관한 비교연구

김승곤,김호훈,김태전 서울保健大學 1990 論文集 Vol.10 No.1

Studies were carried out to evaluate the actual status of the carriers infected with N. gonorrhoeae among the high-risk populations over the country and to determine the drug resistance patterns of β-lactamase producing N. gonorrhocae isolates, in particular attention to the recent increasing discoveries of Penicillinase producing Neisseria gonorrhoeae strains this country. The results could be summerized as follows: 1. The isolation rate of N. gonorrhoeae from the suspicious gonorrhoeal patients (men) were 37.5% on the average, out of a total of 817 examined, but from the prostitutes or bar-girls the isolation rate showed 9.4% on the average out of 3.609 examined, of the isolated 644 gonorrhoeal strains, one hundred and fifty-three strains were detected as β-lactamase producing N. gonorrhoeae. The average detection rate of β-lactamase producing N. gonorrhoeae was 23.8%, but the frequency of PPNG detection was much different depening on the areas where the specimens collected ranging from 3.8%(Chun buk do) to 41.2%(Kyung buk do), except the area Jaeju island where on PPNG was detected. 2. Most non-PPNG strains were able to be inhibited at the concentration of the drugs below 32ug/ml of Penicillin G and 8ug/ml of Ampicillin, but nearly half or more than half of the β-lactamase producing N. gonorrhoeae strains were not inhibited at 32ug/ml of the drugs, In other words, β-lactamase producing N. gonorrhoeae had acquired high resistance to Pehicillin G and Ampicillin. 3. In susceptibility of PPNG and non-PPNG to spectinomycin, Kanamycin, Tetracycline, Erythromycin, Cefuroxin, Cefoperazone, it seemed to be no significant diffevence, but some exceptions showing a peculiar drug resistance to 16ug/ml of Cefuroxin, 64ug/ml of Kanamycin, 512ug/ml of Spectinomycin or 32ug/ml of Tetracycline. And also a few multi-resistance like PPNG strains could rarely be detected showing resistance to the concentration of Tetracycline 32ug/ml, Cefuroxin 16ug/ml, Kanamycin 64ug/ml. 4. In the correlation of inhibitory Zone size dise diffusion method on the MICS by agar plate dilution method to 89 N. gonorrhoeae strains with Pencillin G, Ampicillin, Erythromycin, Tetracycline, and chlorampenicol, Ampicillin showed the highest correlation coefficient as an absolute value showing r=-0.7598, which had negative correlation. Through these studies β-lactamase producing N. gonorrhoeae strains were able to be found from the specimens collected through all over the provinces of the country except the Island of Jaejudo hopsitols.

한국산 족부 백선균의 항진균제 감수성에 관한 연구

김승곤,김태전,이태수 서울保健大學 1997 論文集 Vol.17 No.1

Minimum inhibitory concentration(MIC) of griseofulvin, imidazole derivatives such as ketoconazole, econazole, miconazole and itraconazole on 52 strains of Trichophyton ruhrum, 22 strains of T. mentagraphytes, 10 strains of Microsporum spp. and 1 strain of Epidermophyton floccosum were determined after one week of incubation at 25C on Sabouraud dextrose broth. The results were summarized as followes : 1. The MICs of griseofulvin against 4 different tinea pedis fungi were ranged from 10-100mcg/ml. This values indicated that effectiveness of antifungal activity were relatively lower than those of three other imidazole derivatives such as ketoconazole, econazole, miconazole and one itraconazole. 2. Out of three imidazole derivatives, econazole had relatively higher inhibitory concentrations with 0.1-10mcg/ml against all tinea pedis isolated. 3. Out of 5 antifungal agents, itraconazole which is trizole derivatives showed the highest inhibitory activity with 0.1-10mcg/ml against all tinea pedis tested. 4. These results indicated that inhibitory activity of griseofulvin against tinea pedis were lower than those of four other antifungal agents tested. But these four antifungal agents such as ketoconazole, econazole, miconazole and. itraconazole were effective against tinea pedis tested.

토양에서 분리된 방선균들에 의한 Rimazol Brilliant Blue R(RBBR) 염료의 탈색에 질소원과 pH가 미치는 영향

김태전,김승곤,윤경하 서울保健大學 1997 論文集 Vol.17 No.1

Many workers had reported that the degradation of lignocellulose and lignin by white rot fungi were significantily correlative with the degradation of phenol or PAHs (Polycyclic aromatic hydorcarbons) compounds and the decolorization of a various dyes, specially RBBR (Remazol brilliant blue R) and Poly R-478 dyes. but it were not known distinctly that the degradation of these and the decolorization of these by Actinomycetes were related to the degradation of phenol or PAHs compounds, and author had examined in relationship between the degradation of lignocellulose or lignin and the decolorization of RBBR by Actinomycetes isolated in 67 soil samples collected at 8 areas of Korea from February, 1994 to March, 1995. In the experiment, There were selected two strains identified as Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 which had good correlation between the degradation of lignocellulose or lignin, and it were investigated that the cultural parameters to maximize the decolorization ratios of RBBR by two strains, as the results, Streptomyces halstedii scabies SA1-36 revealed each the highest decolorization ratios with 73.4% in 0.30% glycerin and Streptomyces lavendulas SA2-14, with 74.1% in 0.25% cellobiose at 30℃ in 20 days of culture. There were used their results as basal data in this study that examined the effects of nitrogen sources and pH to maximize the decolorization ratios of RBBR. It were summarized as following that the results examined the effects of nitrogen sources and pH to maximize the decolorization ratios of RBBR by two strains. 1) It were the highest each as 75.7% in casein and 75.7% in yeast extract that the dicolorization ratios of RBBR by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains according to nitrogen sources(0.l%). 2) It were the highest each as 76.8% in 0.1% casein and 78.3% in yeast extract that the decolorization ratios of RBBR by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains according to the concentrations of casein and yeast extract. 3) It were the highest each as 76.8% in pH 7.6 and 79.4% in pH 6.6 that the decolorization ratios of RBBR according to pH by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains. In the above results, we could knowe that it were each different as well as carbon sources that nitrogen sources and pH required between Streptomyces halstedii scabies SAl-36 and Streptomyces lavendulas SA2- 14 strains. and organic nitrogens influence more than inorganic nitrogens to decolorize RBBR dye and nitrogen sources and pH influence lower than carbon sources to decolorize RBBR dye. What`s more, we could knowe that it were very important to use newly formuliged optimal basal media maximize decolorization ratios of RBBR dye by Streptomyces halstedii scabies SAl-36 and Streptomyces lavendulas SA2-14 strains.

척수압박으로 발현된 급성 골수성 백혈병에 동반된 과립구성 육종 1 례

윤상준,김영곤,김희종,박유환,정춘해,김양수,김태균,박영진,전호종 조선대학교 부설 의학연구소 2000 The Medical Journal of Chosun University Vol.25 No.1

저자들은 척수압박으로 하지마비를 보인 환자에서 수술 후에 급성 백혈병에 동반된 과립구성 육종으로 진단된 환자 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Granulocytic sarcoma(GS) is an uncommon tumor composed of immature cells of the granulocytic series. Most case of GS occur in the course of acute leukemia and the blast crisis of chronic leukemia, Rarely, however, it may present before leukemia becomes clinically apparent. It may also occur in patients with myeloproliferative disoders. GS has been reported that it occurs in 3% to 9% of patients with acute myelogenous leukemia(AML) and the incidence of GS is reported to be higher in patients with t(8;21). GS occurs relatively commonly in africa and has been reported to affect 10-25% of black children presenting with AML. This is very rare case of granulocytic sarcoma with AML(FAB M5) presented with spinal cord compression which was supported by decompression laminectomy.

血液 및 尿中 水銀濃度의 統計學的인 比較 檢討

김승곤,김태전 서울保健大學 1986 論文集 Vol.6 No.1

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

병원내 생리기능 검사실의 적정 업무환경에 관한 연구

배형준,윤기은,김승곤,나동진,문희주,김태전,박정오 서울보건대학 1999 서울보건대학 부설 병원경영연구소 논문집 Vol.4 No.2

The purpose of this study was to suggest guideline for optimal work environment of physiological laboratory in hospital. Therefore this study was carried out to analyze the actual condition of laboratory system, major equipments and persons. The period of survey was from September to November 1997 and the objects were 123 laboratory. The major results of this study were as follows. 1. The optimal areas of general ECG room, exercise ECG room and echocardiology room were all 9-18㎡, that of Holter ECG room was 3-12㎡. The optimal areas of neurosystem laboratory were all 9-12㎡, those of respiratory system laboratory were all 9-18㎡. 2. The optimal number of persons laboratory of hospital 500 beds and less were 2∼3, those of 501∼700 beds were 3∼4.701∼1.000 beds were 4∼5 and over 1.001 beds were 7∼8 persons in circulatory system laboratory. The optimal number of persons laboratory of hospital 500 beds less than were 1∼2, those of 501∼700 beds were 3∼4, 701∼1.000 beds were 6∼7 and over 1.001 beds were over 8 persons in neurosystem laboratory. The optimal number of persons laboratory of hospital 500 beds and less 1. those 501∼1.000 beds were 1∼2 and over 1.001 beds were 2∼3 persons in repiratory system laboratory. 3. The optimal number of equipments of general ECG were 2∼3 in the 500∼1.000 beds hospital. there were 3∼4 units in the 1,001 beds. The optimal number of equipment EEG were both 1∼2 in the hospital 500 beds and less and 501∼700 beds. those were 4∼5 units in hospital over 701 beds. The optimal number of equipments of spirograph were both 1∼2 in the hospital 500 and less and 501∼1.000 beds. those were 2∼3 units in the over 1.001 beds. 4. In view of speciality and legal problem of physiofunctional test. we sugget that non-licensee were so quickly change place licensee in the interests of optimal work environment. 5. We suggest that to upgrade qualitative level of domestic equipment and to the more amplify utilization with respect to domestic equipment for the purpose of reducing loss of foreign money.

尿中 Aminolevulinic Acid와 Porphobilinogen의 비교 조사 연구

나동진,윤기은,김승곤,문희주,김태전,박정오 서울保健大學 1990 論文集 Vol.10 No.1

This studies were examined to measure total ALA and PBG concentration in Urine, and find the relationship of correlation among them in 57 men unexposed and 43 men exposed by mercury. The results were summarized as the following; 1. In 100 populations total urine ALA concentration was 0.85±0.17㎎% in male, in female were 0.40 ± 0.04㎎% (normal value : 0.54㎎%). 2. Urine ALA concentration in male were 1.04∼1.11mg% for the ages between 30 ∼ 49 was the highest and then in female were 0.58㎎% for the ages between 40 - 59 was the highest. 3. In 100 populations total urine PBG concentration was 0.03㎎% in male, in female were 0.02㎎% (normal value : 0.03㎎%). 4. Urine PBG concentration in male were 0.05㎎% for the ages between 30-49 was the highest. 5. In unexposed men group total urine ALA concentration was 0.13±0.03㎎% in male, in female were 0.11±0.0l㎎%. 6. In exposed men group total urine ALA concentration was 1.81±0.55㎎% in male, ill female were 0.78±0.09㎎%, there was differences in attention showing male was higher than the female (P<0.05). 7. In unexposed men group total urine PBG concentration was 0.02㎎% in male, in female were 0.0l㎎%. 8. In exposed men group total urine PBG concentration was 0.06㎎% in male, in female were 0.04㎎%,there was differences in attention showing male was higher than the female (P<0.05).

供血者에서의 HBsAg 發現率과 生化學的 檢査値에 관한 硏究

문희주,윤기은,김승곤,라동진,김태전,박정오,최범열 서울保健大學 1988 論文集 Vol.8 No.1