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Piao, D.C.,Lee, Y.S.,Bok, J.D.,Cho, C.S.,Hong, Z.S.,Kang, S.K.,Choi, Y.J. Academic Press 2016 Protein expression and purification Vol.126 No.-
The emergence of highly pathogenic variant porcine epidemic diarrhea virus (PEDV) strains, from 2013 to 2014, in North American and Asian countries have greatly threatened global swine industry. Therefore, development of effective vaccines against PEDV variant strains is urgently needed. Recently, it has been reported that the N-terminal domain (NTD) of S1 domain of PEDV spike protein is responsible for binding to the 5-N-acetylneuraminic acid (Neu5Ac), a possible sugar co-receptor. Therefore, the NTD of S1 domain could be an attractive target for the development of subunit vaccines. In this study, the NTD spanning amino acid residues 25-229 (S25-229) of S1 domain of PEDV variant strain was expressed in Escherichia coli BL21 (DE3) in the form of inclusion bodies (IBs). S25-229 IBs were solubilized in 20 mM sodium acetate (pH 4.5) buffer containing 8 M urea and 1 mM dithiothreitol with 95% yield. Solubilized S25-229 IBs were refolded by 10-fold flash dilution and purified by one-step cation exchange chromatography with >95% purity and 20% yield. The CD spectrum of S25-229 showed the characteristic pattern of alpha helical structure. In an indirect ELISA, purified S25-229 showed strong reactivity with mouse anti-PEDV sera. In addition, immunization of mice with 20 μg of purified S25-229 elicited highly potent serum IgG titers. Finally, mouse antisera against S25-229 showed immune reactivity with native PEDV S protein in an immunofluorescence assay. These results suggest that purified S25-229 may have potential to be used as a subunit vaccine against PEDV variant strains.
( Piao¸ S. Y. ),( Z. R. Li ),( Y. C. Sun ),( J. I. Lee ),( Y. E. Amanor ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2020 강원 농업생명환경연구 Vol.32 No.S
Rice is an important staple cereal for many households in Ghana. This study examines the factors that influence consumers’ preference for rice. Using questionnaires, 300 rice consumers, who were sampled across the municipality, were interviewed. Descriptive statistics, factor analysis, and multinomial logistic regression model were used to analyze the obtained data. The rice-related attributes that showed marked influence on consumers’ preference for rice were packaging quality, swelling capacity, stickiness of grains after cooking, percentage of broken grains, market availability, price, and degree of whiteness. Therefore, the stakeholders should implement strategies to improve these rice-related attributes and enhance the rice post-harvest chain.
Lee, S.,Park, S.K.,Choi, E.,Piao, Y. Elsevier Sequoia 2016 Journal of electroanalytical chemistry Vol.766 No.-
<P>A novel electrochemically deposited graphene (EG)/bismuth (Bi) nanocomposite film-modifiedglassy carbon electrode (GCE) was prepared through successive electrodeposition of exfoliated graphene oxide (GO) and in situ plating bismuth film. Experimental variables, including deposition potential and bismuth concentration, were carefully optimized to determine trace zinc, cadmium, and lead ions by using the EG/Bi electrode as an electrochemical-sensing platform. The linear calibration curves ranged from 1 to 100 mu g L-1 for Zn2+, Cd2+, and Pb2+. The detection limits were 1.80 mu g L-1 for Zn2+, 0.18 mu g L-1 for Cd2+, and 0.11 mu g L-1 for Pb2+ (S/N = 3). The EG/Bi nanocomposite film electrode was successfully applied to the analysis of trace metals in real environments. (C) 2016 Elsevier B.V. All rights reserved.</P>
Yoo, S.,Lee, J.,Kim, J.M.,Seong, C.Y.,Seong, K.d.,Piao, Y. Elsevier Sequoia 2016 Journal of Electroanalytical Chemistry Vol.780 No.-
<P>For large scale batteries, sulfur is an attractive cathode active material for lithium batteries because of high theoretical capacity of 1675 mA hg(-1). However, severe capacity fading and low conductivity of sulfur are significant challenges for its practical application. Here, we report a facile approach to prepare reduced graphene oxide nanoscroll embedded with well-dispersed sulfur (S/GNSC) through a one-pot oxidation of sulfide and reduction of graphene oxide followed by freeze-casting process to generate graphene nanoscroll. The S/GNSC takes the novel shape of reduced graphene oxide sheet spirally wrapped into one-dimensional tubular structure, which enhances electrochemical performance by acting as physical barrier to prevent polysulfide from dissolving and an effective network to promote electron and Li+ transport during reaction. Therefore, the S/GNSC composite with high sulfur loading of 76 wt% exhibits high initial capacity of 1295 mA h g(-1) and reversible capacity of 744 mA h g(-1) after the 100th cycle at 0.2 degrees C when it is used as a cathode material for lithium-sulfur battery, while the capacity of sulfur loaded on reduced graphene oxide sheet (S/rGO) is continuously fading to 510 mA h g(-1) after the 100th cycle due to the irreversible loss of polysulfide. (C) 2016 Elsevier B.V. All rights reserved.</P>
Lee, S.,Oh, J.,Kim, D.,Piao, Y. Pergamon Press ; Elsevier Science Ltd 2016 Talanta Vol.160 No.-
We report an analytical assessment of an iron oxide (Fe<SUB>2</SUB>O<SUB>3</SUB>)/graphene (G) nanocomposite electrode used in combination with in situ plated bismuth (Bi) working as an electrochemical sensor for the determination of trace Zn<SUP>2+</SUP>, Cd<SUP>2+</SUP>, and Pb<SUP>2+</SUP>. The as-synthesized nanocomposites were characterized by transmission electron microscopy, scanning electron microscopy, thermo-gravimetric analyzer, and X-ray diffraction. The electrochemical properties of the Fe<SUB>2</SUB>O<SUB>3</SUB>/G/Bi composite modified electrode were investigated. Differential pulse anodic stripping voltammetry was applied for the detection of metal ions. Due to the synergetic effect between graphene and the Fe<SUB>2</SUB>O<SUB>3</SUB> nanoparticles, the modified electrode showed improved electrochemical catalytic activity high sensitivity toward trace heavy metal ions. Several parameters such as the preconcentration potential, bismuth concentration, preconcentration time, and pH were carefully optimized to determine the target metal ions. Under optimized conditions, the linear range of the electrode was 1-100μgL<SUP>-1</SUP> for Zn<SUP>2+</SUP>, Cd<SUP>2+</SUP>, and Pb<SUP>2+</SUP>, and the detection limits were 0.11μgL<SUP>-1</SUP>, 0.08μgL<SUP>-1</SUP>, and 0.07μgL<SUP>-1</SUP>, respectively (S/N =3). Repeatability (% RSD) was found to be 1.68% for Zn<SUP>2+</SUP>, 0.92% for Cd<SUP>2+</SUP>, and 1.69% for Pb<SUP>2+</SUP> for single sensor with 10 measurements and 0.89% for Zn<SUP>2+</SUP>, 1.15% for Cd<SUP>2+</SUP>, and 0.91% for Pb<SUP>2+</SUP> for 5 different electrodes. The Fe<SUB>2</SUB>O<SUB>3</SUB>/G/Bi composite electrode was successfully applied to the analysis of trace metal ions in real samples. The solventless thermal decomposition method applied to the simple and easy synthesis of nanocomposite electrode materials can be extended to the synthesis of nanocomposites and promising electrode materials for the determination of heavy metal ions.
Liu, Z.H.,Yang, F.Y.,Kong, L.J.,Lai, C.H.,Piao, X.S.,Gu, Y.H.,Ou, X.Q. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.10
A single factorial experiment was conducted to test the effects of three dietary levels of energy on mRNA expression of fatty acid synthase (FAS-mRNA) and hormone-sensitive lipase (HSL-mRNA) and their association with intramuscular fat in finishing pigs. 72 crossbred (Large $White{\times}Rongchang$) barrows with an average initial body weight of 20.71 (s.e. 0.1) kg, were randomly allotted to three dietary treatments (11.75, 13.05 and 14.36 MJ DE/kg) and fed until slaughtered at 100 or 101 kg. The diets were iso-nitrogenous and iso-essential amino acids. The growth performances including the duration of finishing were changed linearly (p<0.05) or quadratically (p<0.05) with increased dietary energy levels. The effects of dietary energy content on the percentage of external fat, intramuscular backfat and the fat thickness were linear (p<0.05). The content of dietary energy increased FAS-mRNA linearly or quadratically, while HSL-mRNA decreased linearly or quadratically in backfat and Longissmus dorsi muscle. Meanwhile, significant positive correlations (p<0.05) were found between energy level and intramuscular fat, FAS-mRNA or the ratio of FAS-mRNA to HSL-mRNA, between the ratio of FAS-mRNA to HSL-mRNA and intramuscular fat. However, the correlations between HSL mRNA and dietary energy or intramuscular fat were negative (p<0.05). The results indicated that dietary energy level regulates lipid accumulation, especially intramuscular fat, possibly by modulating the mRNA of FAS and HSL together rather than individually.