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- 저자 : ( Ritsuko Hatozaki ) (검색결과 1 건)
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( Kenji Furukawa ),( Pham Khac Lieu ),( Hiroyuki Tokitoh ),( Ritsuko Hatozaki ),( Takao Fujii ) 한국물환경학회 ( 구 한국수질보전학회 ) 2005 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2005 No.-
Cultivation of extremely slow growing anammox bacteria was succeeded using up-flow column reactor packed with novel polyester non-woven as biomass carrier. Characterization of an anammox community on a polyester nonwoven attachment medium revealed an organism with a 16S rDNA sequence similarity of 92.2% to the archetypal Candidatus B. anammoxidans. The newly discovered strain (KSU-1, AB057453) was dominant (72.8%) among detectable members of a biofilm community. By fluorescence imaging, KSU-1 was shown to form spherical granular clusters wrapped in a thin layer of Zoogloea sp. This type of anammox reactor was successfully scale-up to the working volume to 50 L. Maximum total volumetric nitrogen removal rate of 1.68kg-N/㎥/d was achieved for the 4 months continuous operation. For the application of anammox reaction to the nitrogen removal process, half of influent NH4-N must be converted to NO2-N as a preceding step for anammox reaction. Operational conditions for this partial nitritation treatments using newly designed attach-immobilized reactor with the volumetric loading rate of 0.48 kg-N/㎥/d. were determined to be pH 7.5 to 7.7 at 35℃ experimentally. Anammox bacteria grew inside of the attach-immobilized sludge during the long term treatment of partial nitritation and influent NH4-N was successfully removed to dinitrogen gas only in one reactor. This newly discovered one stage NH4-N removal process was named as Single-Stage Nitrogen Removal Using Anammox and Partial Nitritation (SNAP) process. This SNAP reactor was operated under operational conditions of pH 7.5-7.7, 35℃ and DO 2-3㎎/ℓ, and 60 to 80% of influent NH4-N were removed under NH4-N loading rate of 0.4kgN/㎥/d. Through the DNA analysis of the sludge attached on net type acryl fiber biomass carrier, it was made clear that ammonium oxidizers and anammox bacteria coexisted in the same attach-immobilized sludge on biomass carriers. Favorable cultural condition for the growth of anammox bacteria was created inside attach-immobilized nitrifying sludge. Two kinds of anammox bacteria and two kinds of ammonium oxidizers were detected in the SNAP sludge. Exiting ratios of anammox and ammonium oxidizing bacteria were estimated to be 15% and 8.7%, respectively, based on the obtained clone numbers in the PCR analysis for SNAP sludge. This coexisting condition was confirmed by the confocal scanning electron microscope on the FISH image of SNAP sludge.
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