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Surveillance of Burkholderia spp. in Bukit Merah Orang Utan Island (BMOUI), Perak, Malaysia
Nurul Iman Mohamad,Nurul Aili Zakaria,Sabapathy Dharmalingam,Zakuan Zainy Deris,Farida Zuraina Mohd Yusof 한국미생물학회 2022 미생물학회지 Vol.58 No.3
Melioidosis is an infection caused by Burkholderia pseudomallei and it represents one of the most common infectious diseases in zoo and farm animals giving rise to high mortality, leading to biological diversity loss. We use the bacteriological and biochemical methods such as culture, biochemical test, Gram-staining and oxidase test to examine the presence of B. pseudomallei in 50 soils treated and non-treated with quicklime powder collected from Bukit Merah Orang Utan Island, (BMOUI), Perak, Malaysia. We observed mixed bacterial colony growth with various colony morphologies. Six of the isolates labelled as P2(25), P5(2), P5(3), P5(5), P6(1), and P6(6)2 showed pinkish purple colour with wrinkled characteristics similar to B. pseudomallei colony morphology after incubation for 48 h. The results showed that all putative soil-isolated organisms were Gram-negative bacteria, positive for oxidase test and negative for catalase test. In the VITEK® ID system and PCR sequencing analysis, no B. pseudomallei was identified. All six putative organisms were identified as in B. cepacia complex i.e.: B. stagnalis (four cases), B. contaminans (one) and B. multivorans (one). These preliminary findings demonstrate that a few species of B. cepacia group were successfully isolated from the soil of BMOUI using selective media for B. pseudomallei.
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( Supardy Nor Afifah ),( Darah Ibrahim ),( Shaida Fariza Sulaiman ),( Nurul Aili Zakaria ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.6
The inhibitory effect of the Klebsiella pneumoniae ATCC 13883 strain caused by the hexane extract of Halimeda discoidea (Nor Afifah et al., 2010) was further evaluated by means of the microscopy view and its growth curves. The morphological changes of the K. pneumoniae ATCC 13883 cells were observed under the scanning electron microscope (SEM) and transmission electron microscope (TEM) after they were treated at minimum inhibitory concentration (MIC; 0.50 mg/ml) (Nor Afifah et al., 2010) for 12, 24, and 36 h. The results showed the severity of the morphological deteriorations experienced by the treated cells. The killing curve assay was performed for 48 h at three different extract concentrations (1/2 MIC, MIC, and 2 MIC). An increase in the extract concentration of up to 2 MIC value did significantly reduce the number of cells by approximately 1.9 log10, as compared with the control. Identification of the potential compounds of the extract responsible for the antibacterial activity was carried out through the gas chromatography-mass spectrum (GCMS) analysis of the active subfraction, and the compound E-15-heptadecenal was identified and suggested as the most potential antibacterial compound of this extract. The subsequent cellular degenerations showed by the data might well explain the inhibitory mechanisms of the suggested antibacterial compound. All of these inhibitory effects have further proven the presence of an antibacterial compound within H. discoidea that can inhibit the growth of K. pneumoniae ATCC 13883.
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