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K. E. Nandini,Navin K. Rastogi 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.2
Downstream processing of lipase involving reverse micellar extraction of lipase using cationic surfactant cetyltrimethylammonium bromide (CTAB) was investigated. Effect of various process parameters on both forward and backward extraction of lipase from crude extract was studied to optimize its yield and purity. Complex interaction of salt concentration (0.05~0.15M), surfactant concentration (0.10~0.30 M), and pH (6.0~9.0) for forward extraction, as well as, salt concentration (0.5~1.5 M) and pH (6.0~9.0)for backward extraction have been studied using response surface methodology. Optimum processing conditions,namely, salt concentration 0.16M, surfactant concentration 0.20 M, and pH 9.0 for forward extraction, as well as, salt concentration 0.80 M and pH 7.23 for backward extraction,fulfill the conditions to obtain activity recovery of lipase ≥78% and purification factor of lipase ≥4.0. The study demonstrated that response surface methodology can be used for optimization of the conditions for reverse micellar extraction of lipase.
( Rani Mahadevaswamy Usha ),( Navin K. Rastogi ),( K. A. Anu Appaiah ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.7
During the production of grape wine, the formation of thick leathery pellicle/bacterial cellulose (BC) at the air-liquid interface was due to the bacterium, which was isolated and identified as Gluconacetobacter hansenii UAC09. Cultural conditions for bacterial cellulose production from G. hansenii UAC09 were optimized by central composite rotatable experimental design. To economize the BC production, coffee cherry husk (CCH) extract and corn steep liquor (CSL) were used as less expensive sources of carbon and nitrogen, respectively. CCH and CSL are byproducts from the coffee processing and starch processing industry, respectively. The interactions between pH (4.5- 8.5), CSL (2-10%), alcohol (0.5-2%), acetic acid (0.5- 2%), and water dilution rate to CCH ratio (1:1 to 1:5) were studied using response surface methodology. The optimum conditions for maximum BC production were pH (6.64), CSL (10%), alcohol (0.5%), acetic acid (1.13%), and water to CCH ratio (1:1). After 2 weeks of fermentation, the amount of BC produced was 6.24 g/l. This yield was comparable to the predicted value of 6.09 g/l. This is the first report on the optimization of the fermentation medium by RSM using CCH extract as the carbon source for BC production by G. hansenii UAC09.