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      • P 37 : Comparison between Sputum culture and pharyngeal mucus culture in children

        ( Mitsuhiro Hori ),( Masami Inayoshi ),( Keiko Hasui ),( Masaaki Sasano ) 대한임상병리사협회 2015 임상미생물검사학회 발표자료집 Vol.2015 No.-

        Background Sputum culture is important to the decision of the causative organism of lower respiratory infectious disease. However, pharyngeal mucus culture is often substituted in the children because sputum sampling is difficult. The objective of the study was to examine the difference of detection rate of S. pneumoniae , H. influenzae and M. catarrhalis in the sputum culture and pharyngeal mucus culture. Subject Sample were obtained from 121 children (under 15 years old) who submitted sputum culture and pharyngeal mucus culture at the same time from January to July in 2014. Methods Sputum was put in sterile saline and washed for removing a saliva ingredient. The pharyngeal mucus was submitted to using a cotton swab made suspension with sterile saline. Subsequently, it was made a smear and cultured. Results Three above-mentioned strains were isolated in 89 samples. The detection rate of S. pneumoniae, H. influenzae and M. catarrhalis in sputum culture (more than 2+) was 30.3%, 56.2% and 31.5%. On the other hand, its in the pharyngeal mucus culture was 5.6%, 24.7% and 5.6%, respectively. The sensitivity of S. pneumonia, H. influenzae and M. catarrhalis in result between the Gram stain in the sputum and culture were 63.6%, 92.1% and 88.2% whereas the specificity of those were 80.6%, 70.6% and 81.9%, respectively. Conclusion Sputum culture is recommended as a specimen of lower respiratory infectious disease in Japanese pediatrics respiratory infection guidelines 2011. The results to support it were obtained in this examination and suggested that it is desirable to examine it with sputum culture than pharyngeal mucus culture when suspect bacterial pneumonia in the children.

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