삼차신경입쪽핵에서 구강유래 감각정보의 처리양식

박성희,박매자,최갑식 대한해부학회 2003 Anatomy & Cell Biology Vol.36 No.6

韓國人 學生의 腦機能 優勢(Cerebral Dominance)와 손잡이(Handedness)의 傾向

박창근,김은희,박매자,배용철,홍해숙,조희중,주강 慶北大學校 醫科大學 1990 慶北醫大誌 Vol.31 No.4

This study was investigated the effects of specialization of brain functions, the distribution of handedness and the correlation between cerebral dominance and handedness. This was surveyed with the samples of 1,302 Korean boys and girls attending in junior high school and senior high school, residing in Taegu city. In this study, Alfred W. Munzert's questionnaire and Tames Bliss & Joseph Morella's questionnaire were used as the instrument for assessing the thend of cerebral dominance and handedness. The results obtained were as follows: Out of the total number of 1,302 examined, 73.7% of them were regarded as right hemisphere dominant type and 12.6% of them were regarded as left hemisphere dominant type. In right hemisphere dominant type, students of junior high school were higher incidence than senior high School. In bilateral and left hemisphere dominant type, students of senior high school were higher incidence than junior high school. In right handers most of them were right hemisphere dominant type. In left handers, most of them were right hemisphere dominant type and the incidence of boys were higher than girls.

흰쥐 척수 및 등뿌리신경절에서 Tyrosine Hydroxylase 함유구조물의 분포

박매자,배용철,조희중,김지윤,김용주,김지윤 경북대학교 병원 1998 경북대학교병원의학연구소논문집 Vol.2 No.1

정상 흰쥐의 각 분절별 척수 및 등뿌리신경절에서 catecholamine 함유구조물의 분포를 알아보고자 tyrosine hydroxylase(TH)에 대한 항체를 이용하여 면역조직화학적 염색을 실시하였다. 그 결과 TH 함유신경세포는 목척수분절과 엉치척수분절에서 관찰되었다. 목척수분절의 TH 함유신경세포는 첫째와 둘째 목척수분절에서만 관찰되었으며 신경돌기가 잘 발달된 다각형의 세포인 것으로 보아 이들은 뇌줄기의 TH 함유신경세포군이 목척수분절로 연장된 것이라 생각된다. 그러나 엉치척수분절에서 관찰되는 대부분의 TH 함유신경세포는 세포돌기가 보이지 않고 세포체부분만 면역염색되어 관찰되는 것으로 보아 퇴화단계의 세포인 것으로 생각된다. TH 함유신경섬유는 척수의 모든 분절에서 관찰되었으며 이들 TH 함유신경섬유가 각 척수분절의 등뿔보다 배쪽뿔과 중간뿔에서 훨씬 높은 밀도로 관찰되는 것으로 보아 catecholamine은 척수에서 체성감각뿐만 아니라 자율신경계 및 운동신경계의 조절에 관여하리라 생각된다. 등뿌리신경절에서 관찰되는 TH 함유신경세포는 척수의 엉치분절에서 관찰되는 것과 마찬가지로 세포돌기가 거의 보이지 않고 세포체부분만 염색되어 보였다. 또한 등뿌리 신경절의 TH 함유신경섬유는 혈관의 벽을 나선형으로 감고 돌아가는 형태로 관찰되었으며 어느 것도 등뿌리신경절의 신경세포와 연관되어 관찰되지는 않았다. 이로 보아 정상 쥐의 등뿌리신경절에서 catecholamine은 신경세포 사이의 신경전달이나 졸절보다는 신경절 내의 혈류조절과 관계있을 것으로 생각된다. With the aim of gaining more insight into the catecholaminergic system in the nervous system of the rat, we have studied the precise distribution pattern of the tyrosine hydroxylase immunoreactive (TH-IR) fibers and soma in the spinal cord and dorsal root ganglion. In the dorsal root ganglion (DRG) TH-IR fibers were observed to run along the vessel wall, spirally and not found in the neural tissue itself. A few TH-IR fibers were found in the spinal nerve, not in the ventral root. Many TH-IR neurons were distributed in the L3, 4, 5, and 6 DRG but none of them were found in the other DRG segments. In the spinal cord, TH-IR fibers have shown sparse distribution all over spinal cord but relatively dense distribution in the ventral hom, intermediolateral column, lamina I of the dorsal horn of the cervical, lumbar, sacral, and coccygeal segment. TH-IR neurons were found in the interrmediolateral column, dorsal gray commissure, dorsal horn of the C1 and C2 segments and S1-4 segments. TH-IR neurons in the cervical segments were polygonal or spindle shaped with well developed processes. In contrast to this, TH-IR neurons in the sacral segments were oval or spindle shaped withe no processes. In conclusion, neurons in the DRG were not influenced by catecholaminergic nervous input. Intrinsic catecholaminergic nervous systems were found in both of spinal cord and DRG.

호흡 부전을 동반한 급성 자율-감각신경병증 1예

박경필,안규환,김은주,김대성,정대수,박규현,김매자 대한신경과학회 1999 대한신경과학회지 Vol.17 No.5

Xenopus 초기 발생 중 시간에 따른 FGF 신호차단이 내배엽형성에 미치는 영향

차상욱,박매자 대한해부학회 2003 Anatomy & Cell Biology Vol.36 No.6

週齡에 따른 생쥐 Leydig세포의 전자현미경적 구조

서의수,박매자,김덕훈,주강 慶北大學校 醫科大學 1990 慶北醫大誌 Vol.31 No.3

주령(週齡)의 증가에 따른 생쥐 Leydig세포의 광학현미경형태와 초미세구조에 관한 변화는 다음과 같다. 1. 생후 1주군에서 미분화된 Leydig세포는 정세관 주위에서 관찰할 수 있었고, 핵은 방추형이며, 핵질과 핵막에는 이염색질이 많이 분포하고, 세포질에는 소포체와 사립체가 조금 분포하나 그 이외의 세포소기관은 거의 없었다. 2. 3주군에서의 Leydig세포는 아직 완전히 분화되지는 않았으나 정세관 주위에서 관찰되었다. 핵은 난형이며, 핵막의 일부 함입과 이염색질의 부착이 있고 세포질에는 잘 발달된 무과립성 내형질망과 membrane whorl이 있으나 그 이외 세포소기관의 발달은 미약하였다. 3. 5주군에서는 완전히 분화한 Leydig세포들의 군집이 정세관 주위에서 관찰되었다. 핵은 거의 원형이며, 핵에는 진염색질이 분포하며, 세포질에는 사립체의 발달이 현저하고, 그 이외의 세포소기관은 약간 증가하였다. 4. 7주군에서는 잘 발달된 정세관의 구조와 잘 분화된 Leydig세포들의 군집을 tubule의 각 공간에서 관찰할 수 있었다. 세포는 핵이 원형으로, 핵막은 뚜렷하고 세포질에는 무과립성 내형질망, 지방소적, 용해소체, 사립체, 당원입자 등이 아주 잘 발달되어 있었다. This study was performed to investigate the ultrastructure of the Leydig cell on aging of the mouse. The results obtained were as follows. One week after birth: The undifferentiated Leydig cells are found around seminiferous tubules. The nucleus showed the fusiform shape. The heterochromatin was found to be adherent to the nuclear membrane and to be dispersed in the nucleoplasm. The smooth endoplasmic reticulum and mitochondria were poorly developed, and the other cell organelles did not appear at this stage. The cytoplasmic vacuolations began to appear. After 3 weeks: Not fully differentiated Leydig cells are present around tubules. The nucleus had oval shape, and some nuclear membrane was caved and adhered to the heterochromatin. The smooth endoplasmic reticulum and membrane was caved and adhered to the heterochromatin. The smooth endoplasmic reticulum and membrane whorl were well developed. But the other cell organelles were poorly developed. After 5 weeks: Clusters of fully differentiated Leydig cells are found around the tubules. The nucleus had round shape, and the nucleoplasm included an euchromatin. Numerous mitochondria were ovserbed at this stage. After 7 weeks: Clusters of fully differentiated Leydig cells are located in the interspace of each tubules. The shape of nucleus was round, and nuclear membrane was prominent. The smooth endoplasmic reticulum, lipid droplets, lysosomal dense body, mitochondria, ribosome and glycogen were increased markedly in number.

흰쥐 하부뇌간에서의 Cholecystokinin octapeptide의 분포

오석희,박매자,조희중,주강 慶北大學校 醫科大學 1990 慶北醫大誌 Vol.31 No.3

흰쥐의 하부뇌간에서 cholecystokinin-octapeptide(CCK-8)의 분포를 관찰하기 위하여 Peroxidase-Antiperoxidase법으로 염색하여 관찰해 본 결과 다음의 많은 부위에서 CCK-8양성세포 및 양성섬유를 관찰할 수 있었다. 중뇌: 흑질, 복측피개야, n. linea rostralis, 중심회백질, n. parabrachialis colliculi posterioris, 하구 및 외측모대핵에서 CCK-8양성세포가 관찰되었고 양성섬유의 경우 각간핵, n. parabrachialis co lliculi posterioris, parabigeminal n., 하구, 외측모대핵 및 배측 봉선핵에서 관찰되었다. 후뇌: CCK-8양성세포는 n. parabrachialis lateralis, n. of group O, 고속핵, 최후야 및 삼차신경 척수로핵에서 분포하였고, 양성섬유는 교봉선핵, 교핵, n. parabrachialis lateralis, 청반핵, n. parolivaris superior, 와우신경핵, 고속핵, 미주신경배측핵, inferior olivary complex, 박속핵, 설상속핵, 삼차신경척수로핵 및 망양체의 일부등에서 존재하였다. In order to investigate the distribution of immunoreactive cholecystokinin octapeptide(CCK-8)-containing neurons in the lower brain stem of the rat, brains of the rats perfused with fixative were extirpated, fronzen sectioned and processed for Peroxidase method. The results obtained were as follows. Mid brain; CCK-8-containing cells were found in the ventral tegmental area, substantia nigra, nucleus(=n.) linea rostralis, substatia grisea centralis, n. parabrachialis lateralis, inferior colliculus and lateral lemniscus and fibers were distributed in the interpeduncular n., n. linea rostralis, ventral tegmental area, substantia nigra, superior colliculus, substantia gricea centralis, n. parabrachialis, lateralis parabigeminal n., inferior colliculus, lateral lemniscus and n. raphe dorsalis. Pons and Medulla oblongata; CCK-8-containing cells were detected in the n. parabrachialis lateralis, n. of group O, n. tractus solitarii, area postrema and n. tractus spinalis nervi trigemini, and fibers were found in n. centralis superior, pontine n., n. parabrachialis lateralis, locus coeruleus, n. parolivaris superioris, cochlea n., n. tractus solitarii, dorsal n. of vagus nerve, inferior olivary compex, n. gracilis, n. cuneatus, n. tractus spinalis nervi trigemini and reticular formation.

Golgi 염색 기법에 대한 연구

안병권,박매자,김은희,배용철,홍해숙,조희중,주강 慶北大學校 醫科大學 1990 慶北醫大誌 Vol.31 No.4

여러 Golgi methods 중에서 본 실험실 환경에 적합한 방법을 확립하기 위해서, Golgi Method, Golgi-Kopsch modification, silver-chromate Golgi method 그리고 Rapid Golgi method의 네가지 방법을 실제로 실시했는데 그 중 Adam의 silver-chromate Golgi method에서 가장 좋은 결과를 얻었는데 그 결과는 다음과 같다. 1) 착색에 소요되는 시간이 약 7일 정도였다. 2) 대뇌피질, 시상하부, 뇌간의 모든부위 등 실험을 실시한 거의 모든 부위에서 착색된 신경세포를 관찰할 수 있었다. 3) 착색된 신경세포는 세포체 뿐만 아니라 축삭돌기, 수상돌기 그리고 수상돌기소극 등의 미세구조도 잘 관찰되었으며, 규칙적인 분포를 하였다. 4) 착색된 신경교세포와 혈관은 저밀도로 관찰되었다. Four modifications of the Golgi method, i. e. Golgi method, method, Golgi-Kopsch modification, silver-chromate Golgi method and rapid Golgi method were tested to find out most reliable method. Compared to another 3 modifications, Adams' silver chromate Golgi method was found to have several advantages as follows. 1) It took 7 days for impregnation procedure. 2) Neurons were well impregnated and their axons, dendrites and spines could be identified very clearly. 3) Impregnated neurons formed few clusters. 4) Glias and vessels showed very minimal impregnations.

백서에 있어서 incision 중지가 하악두 연골의 성장 및 미세구조에 미치는 영향

성재현,배용철,박매자,주강,경희문 대한치과교정학회 1994 대한치과교정학회지 Vol.24 No.3

The purpose of this study is to investigate the effect of loss of incisal function on the thickness, growth activities, ultrastructure of the condylar cartilage and on the muscle fibers of masseter superficialis, anterior belly of digastric muscle in the growing rats. 37 day-old-rats of which incisors had been trimmed every day received soft diet from weaning and were studied by the autoradiography, electron microscopy and muscle histochemistry. The results obtained were as follows ; The thickness of the fibrous, proliferative layer in superior, posterosuperior portion of the condylar cartilage was significantly(p<0.01) reduced in experimental groups and the decrease rate of fibrous layer thickness was greater in posterosuperior portion than in superior portion of cartilge and was greater than in proliferative layer. In normal group, more cells of posterosuperior portion moved more rapidly towards the medullary cavity. In experimental group, the labelling index of posterosuperior portion was decreased in proliferative layer at 2 hours, in transitional layer at 1, 2 days, in hypertrophic layer at 4 days after injection relative to posterosuperior portion of control group. But labelling index of superior portion was not different form that of control group at all time course after injection. From the muscle histochemistry, the diameter of type IIB fibers in masseter superficialis muscle, type ⅡA, type IIB fibers in anterior belly of digastric muscle decreased significantly(p<0.01) relative to controls in experimental group. From electron microscopic study, in the fibrous layer of the posterosuperior portion of condylar cartilage in normal group, many fibroblast like cells near the joint cavity showed extensive remodelling activities in ultrastructure. There was no morphological changes between experimental and control group in all cartilage cell layers of superior portion but cells near the joint cavity in fibrous layer of posterosuperior portion of experimental group showed morphologically inactive state relative to control group.

위삼차신경핵에서 일차구심성 신경섬유 종말부의 미세구조 및 연접양상

배용철,박국필,정경원,박매자,조희중 대한해부학회 1995 Anatomy & Cell Biology Vol.28 No.5