Paclitaxel inhibits proliferation and promotes apoptosis through regulation ROS and endoplasmic reticulum stress in osteosarcoma cell

Li Ming,Yin Liming,Wu Lili,Zhu Yunsen,Wang Xi 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.4

Background Paclitaxel is a chemotherapeutic drug for cancer, which is isolated from the Pacifc yew tree. However, and the molecular mechanism and the antitumor efects of paclitaxel on osteosarcoma cell remain to be explored. Objective The aim of our study was to explore the possible molecular mechanisms of apoptosis in osteosarcoma induced by paclitaxel. Results Paclitaxel can obviously decrease the proliferation of HOS-732 cells in a dose-dependent manner. Paclitaxel could induce the cell cycle arrest at the G2/M-phase and decreases the CDK5 and CCNE1 expression in HOS-732 cells. Paclitaxel promotes cell apoptosis in HOS-732 cells, which may be contacted to the decreasing of Bcl-2 protein expression. Further, the production of ROS in HOS-732 cells was remarkably increased with the increasing concentration of paclitaxel. Moreover, paclitaxel induces the ER-stress related gene and protein expression (GRP79, DDIT3 mRNA and GRP78, XBP-1 s, IRE1α protein expression) in osteosarcoma cells. Conclusion Paclitaxel can inhibit the proliferation of HOS-732 cells and increase ROS and ER-stress response to promote cell apoptosis, suggesting that paclitaxel may represent a new therapeutic option for the treatment and prevention osteosarcoma.

SYNTHESIS OF ULTRAFINE $Si_3N_4$ PARTICLES BY RF PLASMA CHEMICAL VAPOR DEPOSITION

Hongjie, Zhu,Zhu, Yihua,Han, Jinyi,Li, Chunzhong,Hu, Liming 한국재료학회 1995 Fabrication and Characterization of Advanced Mater Vol.1 No.2

Processing of synthesis $Si_3N_4$ UFP in the RFCVD reactor, using $SiCl_4$ and $NH_3$ as precursors, were experimentally researched. The influence of some operation parameters on the properties of products was investigaed. The nanostructured material made of such $Si_3N_4$ particles was fabricated under highpressure. The results of experiments indicated that it differed from the materials made of coarse $Si_3N_4$ powder on some electric properties.

Business-Driven Process Fragment Selections in RESTful Business Processes

Qinghua Lu,Xiwei Xu,Weishan Zhang,Liming Zhu,Shanshan Li 보안공학연구지원센터(IJUNESST) 2015 International Journal of u- and e- Service, Scienc Vol.8 No.1

Past work showed that runtime adaptability of business processes can be improved by applying Representational State Transfer (REST) to design and implementation of business processes. However, the existing solutions for RESTful business processes (RESTfulBP) were focused on manual selection of process fragments to be composed at runtime. In this paper, we propose solutions that enable semi-automatic selection of process fragment at each decision-making point of RESTfulBP. The new built-in middleware MiniZnMASC can provide user based process fragment advice to knowledge workers in ways that achieve better overall business value while satisfying all existing constraints. In addition, we redesign the architecture of RESTfulBP in order to allow the business-driven decision-making solutions. The solutions are evaluated for feasibility, functional correctness, business benefits, and performance.

Research on aging-related degradation of control rod drive system based on Dynamic Object-Oriented Bayesian Network and Hidden Markov Model

Zhu Kang,Zhao Xinwen,Zhang Liming,Yu Hang 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.11

The control rod drive system is critical to the reactor's reliable operation. The performance of its control system and mechanical system will gradually deteriorate because of operational and environmental stresses, thus increasing the reactor's operational risk. Currently there are few researches on the agingrelated degradation of the entire control rod drive system. Because it is difficult to quantify the effect of various environmental stresses and establish an accurate physical model when multiple mechanisms superimposed in the degradation process. Therefore, this paper investigates the aging-related degradation of a control rod drive system by integrating Dynamic Object-Oriented Bayesian Network and Hidden Markov Model. Uncertainties in the degradation of the control system and mechanical system are addressed by using fuzzy theory and the Hidden Markov Model respectively. A system which consists of eight control rod drive mechanisms divided into two groups is used to demonstrate the method. The aging-related degradation of the control rod drive system is analyzed by the Bayesian inference algorithm based on the accelerated life test data, and the impact of different operating schemes on the system performance is also investigated. Meanwhile, the components or units that have major impact on the system's performance are identified at different operational phases. Finally, several essential safety measures are suggested to mitigate the risk caused by the system degradation

Effect of Thermal Treatment on the Texture and Microstructure of Abalone Muscle (Haliotis discus)

Beiwei Zhu,Xiuping Dong,Liming Sun,Guihua Xiao,Xuejiao Chen,Yoshiyuki Murata,Chenxu Yu 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.6

The texture and microstructure of edible abalone meats were studied during heat treatments from 50to 100^oC for 60 min. No increase in extractable soluble collagen content was observed below 80^oC, but a 9-fold increase was observed at 100^oC. SDS-PAGE showed that extractable myosin heavy chains and paramyosin contents reduced significantly at 80^oC, and disappeared completely at 100^oC. The shear force increased slowly from 50 to 70^oC, but relaxed back to the initial level at 100^oC. Rapid reduction of hardness was observed at 50^oC, minimum hardness was obtained at 100^oC. Springness, cohesiveness,chewiness, and resilience were enhanced to maximum levels at 70, 90, 70, and 90^oC, respectively. Optical micrographs and transmission electron microscope showed a significant increase of intermyofibrillar gaps at 90^oC and broken fibers at 100^oC. Results suggested that 80^oC might be a suitable temperature to produce ready-to-eat abalone products.

Identification of pathways and genes associated with cerebral palsy

Qingwen Zhu,Yufei Ni,Jing Wang,Honggang Yin,Qin Zhang,Lingli Zhang,Wenjun Bian,Bo Liang,Lingyin Kong,Liming Xuan,Naru Lu 한국유전학회 2018 Genes & Genomics Vol.40 No.12

Cerebral palsy (CP) is a non-progressive neurological disease, of which susceptibility is linked to genetic and environmental risk factors. More and more studies have shown that CP might be caused by multiple genetic factors, similar to other neurodevelopmental disorders. Due to the high genetic heterogeneity of CP, we focused on investigating related molecular pathways. Ten children with CP were collected for whole-exome sequencing by next-generation sequencing (NGS) technology. Customized processes were used to identify potential pathogenic pathways and variants. Three pathways (axon guidance, transmission across chemical synapses, protein–protein interactions at synapses) with twenty-three genes were identified to be highly correlated with CP. This study showed that the three pathways associated with CP might be the molecular mechanism of pathogenesis. These findings could provide useful clues for developing pathway-based pharmacotherapies. Further studies are required to confirm potential roles for these pathways in the pathogenesis of CP.

Effects of surfactants on the remediation of petroleum contaminated soil and surface hydrophobicity of petroleum hydrocarbon degrading flora

Jianbo Liu,Liming Xu,Feifei Zhu,Shouhao Jia 대한환경공학회 2021 Environmental Engineering Research Vol.26 No.5

It has been proven that surfactants used in the remediation of petroleum hydrocarbon contaminated soil have great application potential. In this study, the effects of five surfactants (SDBS, Tween80, Tween60, rhamnolipid and TRS-1) on leaching of petroleum hydrocarbons from soil were investigated through orthogonal experiments, and petroleum hydrocarbon components were analyzed by GC/MS. The effects of surfactants on the degradation of petroleum hydrocarbon were analyzed by the changes of microbial growth curve and surface hydrophobicity. The results showed that surfactant type, temperature and surfactant concentration had significant effects on the removal rate of petroleum hydrocarbon. Tween80, rhamnolipid and TRS-1 have good bio-friendliness and a high removal rate of petroleum hydrocarbons (up to 65%), suitable for the restoration of the soil used in the experiment And Surfactants exhibited a higher removal rate for small molecules and petroleum hydrocarbons with odd carbon atoms. Surfactants have a certain modification effect on the surface of relatively hydrophilic bacteria under the initial conditions, making their surface properties develop in the direction of enhanced hydrophobicity, and the hydrophobicity has increased from less than 20% to about 40%.

Changes of Collagen in Sea Cucumber (Stichopus japonicas) During Cooking

Xiuping Dong,Beiwei Zhu,Liming Sun,Jie Zheng,Dan Jiang,Dayong Zhou,Haitao Wu,Yoshiyuki Murata 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.4

Changes of the collagen in sea cucumber (Stichopus japonicas) during cooking were investigated. Crude collagen fibers (CCF) is more sensitive to heat than pepsin-solubilized collagen (PSC), absorbance at 226-232nm increased from 60 to 100^oC. PSC nearly completely degraded after cooking for 8-10 h, 4-6 h, 1-1.5 h, 40-50 min, and 10-20 min at 60, 70, 80, 90, and 100^oC,respectively. Collagen fiber shrinkage, disappearance of periodic cross striation, complete denaturation, and dispersion of denatured fibers at 40, 60, 80 and 100^oC,respectively, were demonstrated by transmission electron microscope (TEM). Above results might be instructional for sea cucumber processing and collagen usage.

NUCLEATION AND GRAIN-GROWTH MODEL FOR REDUCTION OF ACICULATE ULTRAFINE ${alpha}$-$Fe_2O_3$ PARTICLES

Li, Chunzhong,Hong, Zhifu,Zhu, Yihua,Hu, Liming 한국재료학회 1995 Fabrication and Characterization of Advanced Mater Vol.2 No.4

By use of temperature programmed reduction technology, the kinetics of aciculate ultrafine ${alpha}$-F${e_2}{O_3}$</T powder are studied and the optimum conditions of the reduction process are investigated. From this study the kinetic equation can be written as $rac{d{alpha}}{d1}$=9.003${ imes}{10^3}$ exp($rac{-72.66{ imes}{10^3}}{RT}$)(1-${alpha}$)${alpha}^{0.54}$ this equation fits well with experiment results at 570-720K, and the reduction rate of oxide at the surface of the particles increases and the reduction rate of oxide inner the particles decreases with increasing the degree of ${alpha}$-F${e_2}{O_3}$</T eduction. The optimum temperature for magnetic powder preparation is 630-670K.

A Robust Video Fingerprinting Algorithm Based on Centroid of Spatio-temporal Gradient Orientations

( Ziqiang Sun ),( Yuesheng Zhu ),( Xiyao Liu ),( Liming Zhang ) 한국인터넷정보학회 2013 KSII Transactions on Internet and Information Syst Vol.7 No.11

Video fingerprints generated from global features are usually vulnerable against general geometric transformations. In this paper, a novel video fingerprinting algorithm is proposed, in which a new spatio-temporal gradient is designed to represent the spatial and temporal information for each frame, and a new partition scheme, based on concentric circle and rings, is developed to resist the attacks efficiently. The centroids of spatio-temporal gradient orientations (CSTGO) within the circle and rings are then calculated to generate a robust fingerprint. Our experiments with different attacks have demonstrated that the proposed approach outperforms the state-of-the-art methods in terms of robustness and discrimination.