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Comparison of L-Theanine Biosynthesis Enzymes for L-Theanine Production Using Whole-cell Conversion
Kyungjae YU,Byung Wook LEE,Gaeul KIM,Yoon Jung JUNG,Jung Bin SHIN,Jung-Oh AHN,Yung-Hun YANG,Yang KIM,Hyung-Wook JANG,Minjeong SOHN,See-Hyoung PARK,Kyungmoon PARK 한국생물공학회 2024 한국생물공학회 학술대회 Vol.2024 No.4
Kim, Kyujung,Oh, Youngjin,Ma, Kyungjae,Sim, Eunji,Kim, Donghyun Optical Society of America 2009 Optics letters Vol.34 No.24
<P>We investigate optimum plasmon-enhanced total-internal-reflection fluorescence imaging by metallic thin films and nanostructures. The enhancement is based on the mismatch between the conditions of plasmon resonance and maximal near-field intensity. We have calculated plasmon-associated near-field and far-field characteristics using rigorous coupled-wave analysis. Near-field intensity was experimentally measured with fluorescent beads on silver thin films, nanogratings, and nanoislands. The results for nanostructure-based plasmon excitation confirm that momentum mismatching when exciting plasmons can increase the consequent emission of fluorescence substantially. The improvement can be critical depending on the specific structure.</P>
Kim, Kyungmun,Kim, Sang Hyeon,Yoon, Kyungjae Andrew,Cho, Yun Sang,Yoo, Mi-Sun,Lee, Si Hyeock 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.2 No.1
<P><B>Abstract</B></P> <P>The small hive beetle (SHB), <I>Aethina tumida</I>, is an invasive pest species in most Northern Hemisphere countries, including Korea. SHB causes serious damage to apiaries by destroying overwintering honey bee colonies. To obtain basic information for efficient management of SHB, genes encoding conventional insecticide targets, specifically the voltage-sensitive sodium channel α-subunit (VSSC) and acetylcholinesterase (AChE), and RNA interference (RNAi)-related components were annotated and characterized following analysis of transcriptomes of adults and larvae. A single VSSC gene was identified but no apparent mutations associated with pyrethroid resistance were detected. Genes encoding two AChEs (AtAChE1 and AtAChE2) were identified from the SHB transcriptome. No apparent mutations associated with resistance to organophosphorus and carbamate insecticides were identified in the AtAChE1 gene, whereas the S238G mutation, originally identified from the Colorado potato beetle, was detected in the AtAChE2 gene. Native polyacrylamide electrophoresis in conjunction with western blotting revealed that AtAChE1 was the main catalytic enzyme and therefore a toxicologically more relevant target. AtAChE1 was determined to exist in both membrane-anchored and soluble forms. The main components of RNA interference (RNAi) were identified, suggesting that RNAi is likely functional in SHB and an RNAi-based approach is a feasible alternative control measure.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Transcriptome of small hive beetle (<I>Aethina tumida</I>) was analyzed and annotated. </LI> <LI> Insecticide target (<I>Atvssc</I> and <I>Atace</I>) and RNAi-related genes were characterized. </LI> <LI> Of two <I>Atace</I> genes (<I>Atace1</I> and <I>Atace2</I>)<I>, Atace1</I> was determined to encode the major enzyme. </LI> <LI> No apparent mutations associated with insecticide resistance were detected in either <I>Atvssc</I> or <I>Atace1</I>. </LI> <LI> Two additional RNAi-related components (Hen1 and loqs) were newly identified. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Comparative functional analysis of mastoparans from social hornets Vespa crabro and Vespa analis
Kyungjae Andrew Yoon,Kyungmun Kim,Hyo-min Ahn,Ki-Gyoung Kim,Hong-Yul Seo,Young Ho Koh,Si Hyeock Lee 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10
Vespa crabro and V. analis are social hornet species commonly found in Asia, including Korea. Mastoparan is one of the major venom peptides of these two hornets but its amino acid sequence defers substantially. To examine the differences in the potential toxicity and bioactivity of mastoparans between these two social hornets, differential toxicological and pharmacological activities of synthesized mastoparan were investigated. V. analis mastoparan showed a 7-fold higher hemolytic activity, suggesting its higher cytotoxic potential compared with V. crabro mastoparan. Mastoparans from both hornet species exhibited similar levels of antimicrobial activities against Staphylococcus aureus and Botrytis cinerea, whereas the mastoparan from V. analis showed more potent antimicrobial activities against Escherichia coli and Candida albicans. Nevertheless, the antimicrobial activities of mastoparans of V. crabro and V. analis were relatively lower compared with those of other wasps. Both mastoparans also exhibited some levels of antitumor activity but the activity was significantly higher in V. analis mastoparan. In summary, the hemolytic, antimicrobial, and antitumor activities of synthesized V. analis mastoparan were higher than those of V. crabro mastoparan. These differential bioactivities are likely due to the amino acid sequence differences in the mature peptides. In particular, the additional Lys residue present in V. analis mastoparan may contribute to the higher levels of bioactivity as proposed by secondary structure prediction.
Kyungjae Andrew Yoon,Kyungmun Kim,A-Young Kim,Young Han Park,Woo Young Bang,Chang Hwan Bae,Joo-Hong Yeo,Young Ho Koh,Si Hyeock Lee 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.10
Bombolitin is a venom peptide originally isolated from bumblebees and possesses various biological activities, including hemolytic activity. Bombolitins exhibit amphipathic α-helical structure in lipid-membrane-mimicking environments. To investigate their pharmacological and toxicological properties, anti-tumor, anti-microbial and cytotoxic activities of bombolitins from Bombus ardens and Bombus ussurensis were evaluated. Bombolitins of the two species exhibited extremely high anti-tumor activity against ovarian tumor cells SK-OV-3 and NIH; OVCAR-3 at 25-50 μM, which is 2-fold more potent than other wasp venom peptides studied to date (Yoon et al., 2015; Yoon et al., 2016). The two bombolitins showed significantly high antimicrobial activity. However, bombolotin of B. ussurensis showed no antimicrobial activity against Escherichia coli. In addition to their high levels of anti-tumor activity, bombolitins showed considerable levels of hemolytic activity. Thus, to utilize bombolitins as a potential candidate for anti-tumor peptide drugs, further studies for reducing cytotoxic properties of bombolitns is essential.
Specific anti-tumor activities in venom peptides of lesser paper wasp Parapolybia varia
Kyungjae Andrew Yoon,Kyungmun Kim,A-Young Kim,Young Han Park,Woo Young Bang,Chang Mu Kim,Young Ho Koh,Si Hyeock Lee 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04
The lesser paper wasp, Parapolybia varia, belongs to large subfamily Polistinae and is distributed in Middle East, the Indo-Papuan region and East Asia. P. varia is known to become aggressive when disturbed for defending their colonies, resulting in fatal envenomation. Vespid chemotactic peptide (VCP) and vespakinin have recently been determined to be the top two genes most abundantly transcribed in venom glands of P. varia. To investigate the pharmacological and toxicological properties of VCP and vespakinin, their antitumor, antimicrobial, and cytotoxic activities were evaluated. VCP exhibited a significantly high antitumor activity against ovarian tumor cell SK-OV-3 at 100 M. VCP also showed higher hemolytic activity than vespakinin. Antimicrobial activity was only observed with VCP against yeast Candida albicans at 1 mM. Since VCP showed a relatively low hemolytic activity but a considerable level of antitumor activity, it has further merits to be exploited as a potential antitumor agent with reduced side effects on normal cells.
Comparison of Endogenous Cellulase Genes from Four Termite Species with Different Habitats
Kyungjae Andrew Yoon,Young Ho Kim,Si Hyeock Lee 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04
To search for a variety of cellulase genes from termites with different habitats consuming different foods, we collected three species (Neotermes spp., Odontotermes spp., Macrotermes spp.) from the wood and one species (Nasutitermes spp.) from the cow dung. Total RNA was isolated both from alimentary track tissues containing paunch and from other tissues, and used for the suppression subtractive hybridization (SSH). The resulting EST libraries were sequenced and searched by BLAST to identify cellulase genes. A total of 16 cellulase genes were found from the wood-dwelling termites whereas 4 cellulase genes from the cow dung-dwelling termites. Endo-beta-1,4-glucanase and beta-glucosidase were identified as the most abundant cellulase from the wood-dwelling termites and cow dung-dwelling termites, respectively. This finding suggests that cellulase profiles are significantly different depending on the termite’s habitat and food. In addition, we analyzed phylogenetic relationships among the cellulase genes along with other cellulase genes reported to date. All cellulase genes appeared to be originated from endosymbioants without any hint of horizontal gene transfer. Functional expression of endo-beta-1,4-glucanase using a baculovirus expression system is in progress to characterize its enzymatic properties.