필리핀 여행 후 발생한 쯔쯔가무시병 1예

김계형,김낙현,김문석,김충종,전재현,박완범,장원종,박상원,김익상,오명돈,최강원 대한감염학회 2008 감염과 화학요법 Vol.40 No.6

Infectious diseases imported from other countries have increased as more and more Koreans are going abroad for various purposes. Tsutsugamushi disease from other endemic area such as Southeast Asia is important, because it can occur in any season and eschar may be absent. We report a case of imported tsutsugamushi disease acquired in the Philippines. A patient presented with fever, headache, and maculopapular skin rash. However, eschar was absent. Polymerase chain reaction (PCR) for 56-kDa gene of Orientia tsutsugamushi using buffy coat was positive. Serum indirect immunofluorescent antibody assay was initially negative but became positive with a titer of 1:320 at follow-up, Sequencing analysis revealed the strain to be 100% identical to the TW73R strain identified in Taiwan. After the patient received doxycycline, body temperature normalized in 12 hours. Tsutsugamushi disease is one of the differential diagnoses that should be included for patients with fever who have recently returned from Southeast Asian countries. PCR for O. tsutsugamushi using patient's buffy coat was useful for early diagnosis.

Mycobacterium mageritense에 의한 당뇨병성 족부 감염증 1예

김충종,김낙현,김문석,김계형,전재현,박문석,박경운,박완범,박상원,김홍빈,김남중,오명돈,최강원 대한감염학회 2008 감염과 화학요법 Vol.40 No.6

Diabetic foot infection is one of the important complications in patients with advanced diabetes mellitus. Limb threatening infections such as osteomyelitis, abscess, and necrotizing fasciitis are frequently accompanied by the disease. Non-tuberculous mycobacterium (NTM) is a rare causative organism of diabetic foot infection. Thus, if one is not suspicious or meticulous, infection due to NTM will be easily overlooked and this will result in delayed diagnose and treat. Therefore, it is necessary to consider NTM as the causative organism if the wound does not respond to the conventional antibiotic treatment and the culture from the adequately obtained specimen reveals atypical acid-fast bacilli. We present a case of diabetic foot infection with osteomyelitis and abscess due to Mycobacterium mageritense, one of the rapid growing mycobacteria, that was successfully treated with surgical debridement and appropriate antibiotic treatment.

복합영화관에서 화재시 과밀공간 파악을 통한 관객의 피난 행태연구

박계원,이경훈 대한건축학회 2005 대한건축학회논문집 Vol.21 No.1

The purpose of this study is to investigate specific crowding areas resulted from diverse physical factors of multi-plex theaters In a fire evacuation, Identify psychological factors of human behaviors affecting those crowding, and more accurately predict the evacuation route of people To achieve these purposes, the architectural characteristics of three multi-plex theaters have been chosen, and the evacuation experiments through the computer simulation called Simulex and surveys for occupants were also earned out for each on the these theaters Finally, interviewing with fire fighters, the results between the Simulex and the surveys were compared and analyzed The conclusions from this study are as follows (l)Among several human behaviors in the Familiarity, Space using pattern, Turning back, Accessbility, and landmark perception are different in each theater (2)Crowding usually happens cross areas between theater inside and corridors, and Crowding rate depends on the number of cross areas (3)Specialists pointed out the possibility of wayfindind difficulties due to visual accessbilities

일렬 자가조혈모세포이식을 받은 다발성 골수종 환자에서 발생한 뇌 톡소포자충증 1례

김계형,송경호,전재현,박완범,박상원,김홍빈,김남중,김인호,오명돈 대한감염학회 2010 감염과 화학요법 Vol.42 No.3

Toxoplasmosis is a rare but fatal complication in hematopoietic stem cell transplant recipients, usually associated with allogeneic hematopoietic stem cell transplantation (HSCT). We report a case of cerebral toxoplasmosis in a patient with multiple myeloma, following tandem autologous stem cell transplantation. A 55-year-old Korean male presented with weakness in both legs that had progressed to both arms. A magnetic resonance imaging scan of the brain revealed multiple, variablesized ring-enhancing lesions with surrounding edema in the cerebral hemispheres and brain stem. Stereotactic biopsy revealed bradyzoites of Toxoplasma gondii in the brain tissue. The patient received trimethoprim-sulfamethoxazole, followed by pyrimethamine and sulfadiazine, accompanying treatment for progressive multiple myeloma. Cerebral toxoplasmosis should be considered as one of the differential diagnoses in patients with neurologic signs following autologous HSCT.

전정안반사의 임상검사

정원호,박계훈 대한평형의학회 2002 Research in Vestibular Science Vol.1 No.2

진균 세포벽 형성 저해물질 생성 Streptomyces속 세균의 분리 및 수리동정

서원나,박정희,이지영,김인섭,이계준,배무 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

본 연구의 목적은 진균의 세포벽 합성을 저해하는 항진균 물질생성 방선균을 개발하는 데 있다. 다양한 환경으로부터 채취한 토양 시료로부터 방선균 선택 배지를 이용하여 860여 방선균을 선택적으로 분리하였다. 분리 균주 중 Penicillium cyclopium, Cryptococcus laurentii, Aspergillus flavus에 특이적 항균 활성 물질을 생산하는 균주 EMS4, EMP22, L234를 각각 선별하였다. 화학적 동정과 형태학적 동정을 통해 분리 균주들이 Streptomyces속 세균임을 알 수 있었다. 총 50개의 주군집, 34개의 부군집 형질을 분석하고, 이 실험 결과를 TAXON program에 적용하여 종의 수리동정을 실시하였다. 그 결과 분리균 EMS4는 Streptomyces의 제 61 주군집에 속하는 Streptomyces lavendulae의 한 균주로 동정되었고, EMP22는 제 1B 주군집에 속하는 S. willmorei의 한 균주로 동정되었으며, L234는 제 3 부군집의 S. aburaviensis의 한 균주로 동정되었다. The aim of the present research program wa to identify and develop stains of actinomycetes producing antifungal antibiotics which inhibit cell wall biosynthesis. 860 strains of Actionomycetes were isolated from various soil samples. Three isolates, EMS4, EMP22, and L234 were selected as the strains producing antifungal antibiotics inducing abnormal morphology against Penicillium cyclopium. Cryptococcus laurentii, and Aspergillus flavus, respectively. Taxonomic unit characters of the strains were tested and the data were analyzed numerically using TAXON program. EMS4, EMP22, and L234 were identified to be a member of Streptomyces lavendulae, Streptomyces willmorei, and Streptomyces aburaviensis, respectively.

진균 세포벽 형성 저해물질 생성 Streptomyces속 세균의 분리 및 수리동정

서원나,박정희,이지영,김인섭,이계준,배무 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-

본 연구의 목적은 진균의 세포벽 합성을 저해하는 항진균 물질생성 방선균을 개발하는 데 있다. 다양한 환경으로부터 재취한 토양 시료로부터 방선균 선택 배지를 이용하여 860여 방선균을 선택적으로 분리하였다. 분리 균주 중 Penicillum cyclopium, Cryptococcus laurentii, Aspergillus flavus에 특이적 항균 활성 물질을 생산하는 균주 EMS4, EMP22, L234를 각각 선별하였다. 화학적 동정과 형태학적 동정을 통해 분리 균주들이 Streptomyces속 세균임을 알 수 있었다. 총 50개의 주군집, 34개의 부군집 형질을 분석하고, 이 실험 결과를 TAXON program에 적용하여 종의 수리동정을 실시하였다. 그 결과 분리균 EMS4는 Streptomyces의 제 61 주군집에 속하는 Streptomyces lavendulae의 한 균주로 동정되었고, EMP22는 제 1B 주군집에 속하는 S. willmorei의 한 균주로 동정되었으며, L234는 제 3 부군집의 S. aburaviensis의 한 균주로 동정되었다. The aim of the present research program was to identify and develop strains of actinomycetes producing antifungal antibiotics which inhibit cell wall biosynthesis. 860 strains of Actinomycetes were isolated from various soil samples. Three isolates, EMS4, EMP22, and L234 were selected as the strains producing antifungal antibiotics inducing abnormal morphology against Penicillium cyclopium, Cryptococcus laurentii, and Aspergillus flavus, respectively. Taxonomic unit characters of the strains were tested and the data were analyzed numerically using TAXON program. EMS 4,EMP22, and L234 were identified to be a member of Streptomyces lavendulae, Streptomyces willmorei, and Streptomyces aburaviensis, respectively.

축산물 수요모형의 비교와 선정

김태균,박계원,성명환 韓國農業政策學會 1999 농업경영정책연구 Vol.26 No.2

제한효소 SacⅡ의 Isoschizomer인 SexⅢ를 생성하는 토양 Streptomyces 균주의 수리 동정

배무,서원나,박정옥,김형태,이계준 이화여자대학교 생명과학연구소 1993 생명과학연구논문집 Vol.4 No.-

제한효소인 SexⅢ을 생성하는 Streptomyces속의 한 균주(Streptomyces sp.58)를 토양으로부터 분리하여 형태적 관찰 및 수리동정을 실시하였다. 50여개의 분류 단위 형질을 분석하였고 이 실험 결과를 TAXON program에 적용하여 종의 수리 동정을 실시하였다. 그 결과 분리균은 Streptomyces의 제5주군집에 속하는 것으로 나타나 Streptomyces exfoliatus의 한 균주로 동정하였다. Numerical identification was carried out for an isolate of Streptomyces sp. 58 producing a new restriction endonuclease SexⅢ. Fifty taxonomic unit characters were tested and the data were analyzed numerically using the TAXON program. The isolate was identified to the major 5 or Streptomyces. Therefore, it was concluded that the isolate was identified to be a member of Streptomyces exfoliatus.

인공 산성비 용액 함침 및 경사평면법에 의한 옥외용 실리콘 고무의 열화 특성 평가

김정호,정규원,유영식,양계준,박용관 成均館大學校 科學技術硏究所 1999 論文集 Vol.50 No.1

We investigated the effect of acid rain on the surface aging, and resistance to tracking and erosion of silicone rubber materials : First, we measured the contact angle, SEM, EDX, and weight reduction to investigate the surface aging caused by immersion in the artificial acid rain solution. From the results of a series of experiments, we concluded that the acid rain attacked the base polymer of the surface, and lead to the depolymerization. Second, we adopted Inclined-Plane Method to measure the resistance to tracking and erosion of silicone rubber, and rather not observably affected the immersed specimen in the tracking resistance than in the erosion resistance.